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키토산 올리고당의 제조용 소재로서 Bacillus sp. P2l 기원의 키토산분해효소
박노동,조유영,이현철,조종수,조도현 한국미생물 · 생명공학회 1998 한국미생물·생명공학회지 Vol.26 No.4
다양한 중합도의 키토산올리고당의 생산에 적합한 효소원을 개발하기 위해서 23종의 시판효소와 토양에서 분리한 8종의 Bacillus sp.와 1종의 Aspergillus sp.가 생산하는 조효소에서 키토산 분해효소의 활성을 검색하였다. 각 효소의 키토산에 대한 가수분해활성은 탁도의 변화, 반응 후에 생성되는 침전물의 양, 총환원당 생성능력, 점도의 감소 속도 등을 기준하여 평가하였다. 시험한 효소원 가운데서 키토산에 대한 강한 분해활성을 가지는 효소는 P2l이 생산하는 것이었다. 이 균주가 생성하는 chitosanase는, HPLC와 TLC에 의한 분해산물의 분석과, 점도 변화의 측정과 활성염색 등에 근거하여, 주로 내부 가수분해활성을 갖는 것으로 판단되었다. 이 효소는 키토산 올리고당의 생산에 적합한 효소 소재로 가능성이 큰 것으로 평가되었다. In an effort to develop a potent system for the production of various dp (degree of polymerization) chitooligosaccharides, 32 enzymes or microbial systems were screened for chitosanolytic acitivity using chitosan as a substrate. The efficiency of each enzyme system was evaluated by the changes of turbidity and viscosity of chitosan solution, the amount of precipitate and the reducing sugar-producing activity in the enzymatic reaction mixture. Based on these assay methods for the chitosanase activity, Bacillus sp. P2l out of 32 screened systems showed highly potent endochitosanase, which was comparable with a commercially available enzyme (E7). Chitooligosaccharides of dp 3-7 were separated by TLC as major enzymatic reaction products, suggesting that the chitosanase from Bacillus sp. P2l be endo-splitting type.
박노동,조유영,나용균,김종삼 ( Ro Dong Park,Yoo Young Cho,Yong Gyun La,Chong Sam Kim ) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.5
Chitin and chitosan from red crab and squid pen were applied as adsorbents for trapping the dyes in wastewater from dyeworks. We found that the chitin and chitosan were effective adsorbents for the dyes discarded from dyeworks. Chitosan was more effective in dye adsorption than chitin. In a continuous elution column experiment, 1 ㎏ of chitosan could be used for treatment of upto 120 L of wastewater containing 0.05% dye wasted from dyeworks at 75% efficacy, that means 45 g dye was adsorbed per ㎏ chitosan.
강낭콩 잎에서 스트레스에 따른 키틴분해효소의 특이적 유도
박노동,이춘명,전덕영 ( Ro Dong Park,Choon Myong Lee,Deok Young Jhon ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.2
We studied the induction of chitinase (EC 3.2.1.14), a pathogenesis-related protein, in bean plants under stress conditons. The chitinase was barely detected in healthy plant leaves but was significantly induced by ethylene, pathogen Fusarium oxysporum, or wounding, which was tested by enzyme activity assay, western blotting, and activity staining. Six isozymes of bean chitinase were separated in 12.5% SDS-PAGE. They were differentially induced when bean plants were treated with ethylene, F. oxysporum, or wounding.
Demineralization of Crab Shells by Chemical and Biological Treatments
박노동,정우진,김길용,Gyung-Hyun Jo,Ju-Hee Kuk 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.1
To achieve demineralization of crab shell waste by chemical and biological treatments, lactic acid and lactic acid bacterium were applied. In 5.0 and 10% lactic acid, pH rapidly decreased from 6.8 to 4.2 and from 4.5 to 2.4 at day 3, respectively, and thereafter the pH remained at an almost constant level. In a 10% lactic acid bacterium inoculum, pH lowered to 4.6 at day 5. Relative residual ash content rapidly decreased to 49.1 and 16.4% in 5 and 10% lactic acid treatments, respectively, for the initial 12 h. In 2.5, 5 and 10% lactic acid bacterium inoculums, relative residual ash content rapidly decreased to 55.2, 40.9 and 44.7%, respectively, on the first day. Residual dry masses were 76.4, 67.8 and 46.6% in 2.5, 5 and 10% lactic acid treatments, respectively, for the initial 12 h. After a one-time exchange of the lactic acid solution, in the 5.0% lactic acid treatment, residual dry mass rapidly decreased from 66.0 to 41.4%. In 2.5, 5 and 10% lactic acid bacterium inoculums, residual dry masses decreased to 67.6, 57.4 and 59.6% respectively, on the first day. Protein contents after demineralization ranged from 51.354.7% in the chemical treatments and decreased to 32.3% in the lactic acid fermentation process. A negative relationship was shown between pH and demineralization rate in lactic acid and lactic acid bacterium treatments. These results suggest that lactic acid fermentation can be an alternative for demineralization of crab shells, even though the rate and efficiency of the demineralization is lower than the chemical treatment.