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Ly-6A.2 ligand의 발현과 면역반응에서의 기능
최인선,민들레,김영상,이천배 충남대학교 기초과학연구소 1999 忠南科學硏究誌 Vol.26 No.1
To identify and characterize Ly-6A.2 ligand, a chimeric protein of Ly-6A.2 and human IgG C_(r)1 was prepared by transfection with the Ly-GA.2/human IgG chimeric cDNA expression vector into mouse SP_(2)/0 myeloma cells. The purified chimeric protein from a positive clone was used to investigate the expression of Ly-6A.2 ligand on cells from lymphoid tissues. The Ly-6A.2 ligand was expressed on subpopulations of bone marrow, thymus, lymph node, and spleen. For the pursuit of the function of Ly-6A.2 ligand, the spleen cells from Balb/c mouse were splitted into the Ly-6A.2 ligand positive and negative cells. In response to allogeneic antigen in vitro, the Ly-6A.2 ligand negative cells showed much stronger proliferative response than the Ly-6A.2 ligand positive cells. These results suggest that the interaction of Ly-6A.2 and its ligand generates a negative signal for cell proliferation. We propose that the Ly-6A.2 ligand positive cells receive an inhibitory signal from Ly-6A.2 antigen by cell-cell interaction.
결핵항원에 의한 결핵환자 말초혈액 단핵구의 IL-2,IL-10 및 TNF-α mRNA 발현비교
박정규,임영재,김화중,조은경,민들레,임재현,최덕례,박성규 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1
The various clinical features of tuberculosis are mediated by diverse cytokines produced by various immune cells which are initially triggered by M. tuberculosis antigens. CD4+ T cells can be classified into two subsets according to the patterns of cytokines they produce; Thl cells give rise to cell-mediated immunity and are characterized by the production of IL-2 and IFN-y, whereas Th2 cells are more efficient in mediating antibody production and secrete II-4, IL-5, IL-6 and II-10, Thl cells can control Th2 cell and vice versa. Peripheral blood mononuclear cells of healthy, cured and chronic refractory tuberculosis patients were stimulated with PPD, TSP and PHA antigen, and lymphoproliferative response and expression of II-2, IL- 10, TNF-α mRNAs were measured. Lymphoproliferative responses to PPD, TSP and PHA antigen were depressed in chronic refractory case compared with others expression of IL-2 mRNA was depressed in chronic refractory case stimulated with all antigens. Expression of IL-10 and TNF-α were depressed in cured and chronic refractory cases stimulated with PPD and TSP antigens.