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간흡충 감염가토의 담관에 대한 병리조직학적 변화 및 Lectin 반응에 관한 연구
양웅석(Ung Suk Yang),조몽(Mong Cho),백태현(Tae Hyun Paik),김상현(Sang Hyun Kim),주형준(Hyung Jun Joo),이동필(Dong Phil Lee),송근암(Geun Ahm Song),문한규(Han Gyu Moon) 대한소화기학회 1993 대한소화기학회지 Vol.25 No.6
N/A Histopahological and histochemical studies were done in the bile ducts of clonorchis sinensis (CS) infested rabbits. The duration of clonorchis sinensis infestation in the rabbits was between 12 and 17 months and the number of clonorchis sinensis metacercariae which were infested orally was 50-150 per rabbit. Hematoxylin-eosin and avidin-biotin peroxidase complex lectin staining methods were used for this study. Pathological changes of the bile ducts of CS-infested rabbits were papillarv and adenomatous hyperplasia, goblet cells and mucus cells rnetaplasia, ductal wall fibrosis and infitra tion of eosinophils in the ductal wall. These pathological changes were noted both in peripheral and central protion of bile duct. The pathological changes of hyperplasia and metaplasia of ductal epithelium were more marked in the deep gland than surface one, and dysplasia of the epithelium was more marked in the suface gland than deep one. In clonorchis sinensis infested rabbits, the lectins of Soybean agglutinin (SBA), Dolichos biflours agglutinin (DBA) and Ulex europaeus agglutinin (UEA I) were labelled predominant]y in the luminal suface, supranuclear protion of epithelium, goblet cells, hyperplastic cells and metaplastic cells of deep gland . SBA and DBA were labelled weakly to intermediate and UEA I was labelled weakl y to strong, but Peanut agglutnin (PNA) was ]abelled very weakly to those sites in CS-infested rabbits. In non CS infested rabbits, lectins of SBA, DBA, and UEA I were only weakly labelled in the luminal surface of epithelium. In this study, IJEA i which binds specifically glycoprotein of fucose was more pronounced in rabbits infested with CS and so it is postulated that increased activity of fucosyl transferase should be associated with CS infestation.
고양이 위 유문 평활근 세포의 Cholecystokinin Octapeptide에 의한 수축반응에 대한 연구
최성호(Sung Ho choi),신영민(Young Min Shin),백태현(Tae Hyun Paik),김상현(Sang Hyun Kim),송근암(Geun Am Song),조몽(Mong Cho),양웅석(Ung Suk Yang),문한규(Han Gyu Moon),임병용(Byung Yong Rhim) 대한소화기학회 1994 대한소화기학회지 Vol.26 No.2
N/A Single dispersed smooth muscle cells from circular muscle layer of the cat pylorus, iso- lated by enzymatic digestion with collagenase, were used in this experiment to examine the characteristics of contraction by cholecystokinin octapeptide (CCK-OP) and to indentify pharmacologically the existence of cholecystokinin receptors in pyloric sphincter of the cats. Freshly dispersed cells had typical spindle shape and varied widely in length. Some were fully relaxed and others were in various states of contraction. The mean cell length was 80.6+1.9pm with the range of 45-170pm. The dispersed smooth muscle cells from cat pylorus were dose-dependently contracted in response to CCK-OP with the maximum response at a dose 10 %1 CCK OP. Acetylcholine contracted the cells in a similar dose-de- pendent manner with maximum contraction at 10 -10 ?dl acetylcholine. Mucarinic recep- tor antagonist, atropine completely blocked the response to acetylcholine and partially blocked the response to CCKC3P. Specific CCK receptor antagonist, carboxybenzyl-CCK(27 32)-NH; abolished the CCK-OP-induced response but not the acetylcholine-induced con- traction. Neither the response to acetylcholine or to CCK OP was not affected by incuba- tion in Ca''-free Hepes buffer containing 2 mM EGTA. When 4 mM Sr was substituted for Ca in Hepes buffer, the cell shortenning to acetylcholine and CCK-OP were inhibit- ed from 25.4% to 7.1% and from 27.7% to 4.3%, respectively. Based on these results, it is concluded that smooth muscle cells of cat pylorus possess receptors for CCK OP and ace- tylcholine and the receptor-mediated contraction is not dependent on the presence of ex- tracellular calcium and may depend on the release of intracellular Ca from intracellular Ca storage site.(Korean J Gastroenterol 1994; 26: 230 238)
최성호(Seong Ho Choi),신영민(Young Min Shin),김상현(Sang Hyun Kim),박승근(Seung Keun Park),이헌직(Hun Jig Lee),강대환(Dae Han Kang),조몽(Mong Cho),양웅석(Ung Suk Yang),문한규(Han Gyu Moon) 대한내과학회 1993 대한내과학회지 Vol.45 No.1
N/A Background: AFP is known as one of the most sensitive tumor markers for hepatocellular carinoma. But in many cases of hepatocellular carcinoma, low or negative levels of AFP have been observed. And because AFP levels may be increased in cases of other liver diseases such as liver cirrhosis, its specificity and sensitivity are problems. PIVKA-II has been studied as a new tumor marker for hepatocellualr carcinoma with AFP. It is a precursor protein of prothrombin and is converted to active form of prothrombin by the action of Vitamin K dependent carboxylase in hepatocyte microsomes. As this process can be suppressed by Vitamin K deficiency or Vitamin K antogonist such as warfarin, PIVKA-II levels might be increased due to functional derangement of Vitamin K-dependent carboxylation in hepatocellular carcinoma. We measured the levels of PIVKA-II in patients with various liver diseases including hepatocellular carcinoma and evaluated the meaning of increased PIVKA-II levels. Methods: We measured the levels of PIVKA-II in the plasma of 30 patients with hepatocellular carcinoma and 41 patients with other liver diseases by EIA method using monoclonal antibody specific to PIVKA-II. AFP was checked by RIA method. Results: The levels of PIVKA-II were increased above 0.1 AU/mL in the plasma of 25 (83%) patients with hepatocellular carcinoma and 1 patient with liver cirrhosis and 1 chronic hepatitis, 1 receiving warfarin, 2 toxic hepatitis, 2 cholangiocarcinoma and 1 liver metastatis from stomach cancer. In this study, with diagnostic cut-off value of 0.1 AU/ mL, the sensitivity and specificity of PIVKA-II to detect hepatocellular carcinoma were 83.3% and 78% each. With cut-off value of 8.0 AU/ml, the sensitivity and specificity were 66.7% and 100% each. By the combination assay with the cut-off values of AFP above 100ng/mL & PIVKA-II above 0.1 AU/mL the sensitivity for hepatocellular carcinoma could be elevated to 86.7%. Conclusion: We can conclude that PIVKA-II is more sensitive and specific than AFP and is a useful tumor marker which can elevate the sensitivity and specificity to detect hepatocellular carcinoma by combination assay with AFP. And PIVKA-II can be a useful screening method to detect hepatocellular carcinoma arising from liver cirrhosis.