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Plasmid pBR 322 의 Covalently Linked Dimer 형성에 관한 연구
노현모,김용석,장승기 한국유전학회 1983 Genes & Genomics Vol.5 No.1
We observed the shift of the monomer form of pBR 322 in the host E. coli HB 101 to polymer, mainly dimer, in the continuous culture. In order to find the structure of dimer if catenated or covalently linked, the dimer form of plasmid was cleaved partially or completely with either S1 nuclease or Pst I restriction endonuclease, and analyzed by one and two dimensional agarose gel electrophoresis either in the neutral buffer containing ethidium bromide or in alkaline condition. The results suggested that the dimer was covalently linked. We also investigated the pattern of plasmid pEC-3, which is a pBR 322 derivative and grown in E. coli WA 802 (Rec A^+) for many generations. Similar results were obtained. As a possible mechasism for the polymerization, a model was proposed.
노현모 명지대학교 교지편집위원회 1982 明大 Vol.13 No.-
요즈음 '유전공학' 도는 '생명공학' 이라는 말이 신문지상이나 방송을 통해서, 자주 이야기되고 있다. 우리는 유전공학을 미래의 인간을 행복하게 할 위대한 학문이라고 생각하고 있다. 그 이유는 유전공학의 발전으로 식량문제, 질병치료문제, 환경개선문제, 에너지생산문제,채광에 이르기까지의 인간의 의식문제를 근본적으로 해결할수 있는 잠재력을 갖고 있기 때문이다. 이런 문제들을 좀더 쉽게 이해하기 위해서는 '생명'과 '생명현상'에 대해서 알아야 한다.
Blunt - end 를 가진 DNA 의 재조합을 위한 유전자 운반체의 개발
노현모,김용석,송옥규 한국유전학회 1984 Genes & Genomics Vol.6 No.2
A blunt-end DNA cloning vector, pRSK 116, was constructed. This vector was derived from pBR 322 by opening with Sal I restriction endonuclease, filled with only TTP by Klenow fragment, trimmed with S1 unclease, and ligated with T4 DNA ligase. It appeared that the removal of three bases in the middle region of Tc^r gene did not change the genetic property of the marker gene of the pBR 322 and the unique Hinc II site in the Ap^r gene of the modified plasmid pRSK 116 en. dowed improved property for the selection of recombinants. The Hinc II site has been tested for the cloning of blunt-ended human DNA. The recombinant colonies selected were Ap sensitive and Tc resistant and inserted DNAs were easily recovered by Hinc II treatment.
Responsible Region for the Polymerization of Plasmide pEC3
노현모,장승기,이하규,권용태 한국유전학회 1983 Genes & Genomics Vol.5 No.3
RecA protein is known to be one of important factors for the genetic recombination of E.coli and for the polymerization of plasmids in E.coli. We have always observed in prolonged culture the polymerization of plasmid pEC3 in RecA^+ strain of E.coli (WA802, and RRI strain). In order to find if there is the responsible region for the polymerization of plasmids, we have attempted to delete or destroy parts of plasmid by treatment of S1 nuclease and by ligation with T4 ligase. The results showed that the Tc region of the plasmid was mainly responsible for the polymerization. The picture taken by electron microscope showed that the polymerized plasmids were covalently linked.