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신통축어탕(身痛逐瘀湯)이 Hydrogen Peroxide에 의해 손상(損傷)된 배양(培養) 척수감각신경세포(脊髓感覺神經細胞)에 미치는 영향(影響)
이계승,나영훈,차용석,허윤,김도환,한상혁,박병민,이인,문병순,Lee, Kye-Seung,Na, Young-Hoon,Cha, Yong-Suk,Heo, Yun,Kim, Do-Hwan,Han, Sang-Hyok,Park, Byong-Min,Lee, In,Moon, Byung-Soon 대한한방내과학회 2001 大韓韓方內科學會誌 Vol.22 No.4
Objectives : This study was carried out to examine toxic effect of Sintongchukeo-tang on cultured mouse spinal sensory neurons inhibited by neurotoxicity induced by hydrogen peroxide. Methods : MTT assay, NR assay, LDH and neurofilament assay were performed after spinal sensory neurons were preincubater with various concentrations of Sintongchukeo-tang water extract before treatment of cells with hydrogen peroxide. Results : Hydrogen peroxide induced ceil degeneration such as the decrease of cell viability was measured by MTT and NR assay in the cultured mouse spinal sensory neurons. Sintongchukeo-tang water extract was effective in the decrease of LDH activities of neurons produced by hydrogen peroxide. Sintongchukeo-tang water extract was effective in the increase of amount of neurofilaments damaged by hydrogen peroxide. Conclusions : From the above results, it is suggested that hydrogen peroxide induces the inhibition of cell viability in cultured mouse spinal sensory neurons and Sintongchukeo-tang water extract was effective in cultured neurons damaged by hydrogen peroxide.
혼합물설계법에 의한 Li<sub>2</sub>O-TeO<sub>2</sub>-ZnO 유리의 물성에 대한 조성의 가성성인자 분석
정영준,이규호,김태호,김영석,나영훈,류봉기,Jung, Young-Joon,Lee, Kyu-Ho,Kim, Tae-Ho,Kim, Young-Seok,Na, Young-Hoon,Ryu, Bong-Ki 한국재료학회 2008 한국재료학회지 Vol.18 No.11
In this study, the additivity factors of compositions to density and glass transition point ($T_g$) in a $xLi_2O-(1-x)[(1-y)TeO_2-yZnO]$ (0<x<20, 0<y<20) glass system were analyzed by using mixture design, and the change of ionic conductivity with density and $T_g$ was discussed. As a method for predicting the relation between glass structure and ionic conductivity, density was measured by the Archimedes method. The glass transition point was analyzed to predict the relation between ionic conductivity and the bonding energy between alkali ions and non-bridge oxygen (NBO). The relation equations showing the additivity factor of each composition to the two properties are as follows: Density(g/$cm^3$) = $2.441x_1\;+\;5.559x_2\;+\;4.863x_3\;T_g(^{\circ}C)$ = $319x_1\;+\;247x_2\;+\;609x_3\;-\;1950x_1x_3$ ($x_1$ : fraction of $Li_2O$, $x_2$ : fraction of $TeO_2$, $x_3$ : fraction of ZnO) The density decreased as $Li_2O$ content increased. This was attributed to change of the $TeO_2$ structure. From this structural result, the electric conductivity of the glass samples was predicted following the ionic conduction mechanism. Finally, it is expected that electric conductivity will increase as the activation energy for ion movement decreases.
자감초탕(炙甘草湯)이 LPS와 PMA에 의해 손상된 C6 glial 세포에 미치는 영향
조남수,유준기,이인,신선호,문병순,나영훈,Cho, Nam-Su,Rhyu, Jun-Ki,Lee, In,Shin, Sun-Ho,Moon, Byung-Soon,Na, Young-Hoon 대한한방내과학회 2000 大韓韓方內科學會誌 Vol.21 No.3
The water extracts of Jagamcho-tang has been used for treatment of arrhythmia and palpitation in oriental traditional medicine. Brain is provided with blood flow by heart. Jagamcho-tang has been studied on ischemia and infarction in heart. However, little is known about the mechanism by which the water extracts of Jagamcho-tang rescues brain cells from ischemic damages. To elucidate the protective mechanism on ischemic induced cytotoxicity, the effects of Jagamcho-tang on ischemia induced cytotoxicity and generation of nitric oxide(NO) are investigated in C6 glioma cells. Jagamcho-tang induce NO in a dose dependent manner up to 2.5mg/ml in C6 glioma cells. The pretreatment of Jagamcho-tang protect sodium nitroprusside(SNP) (2mM) induced cytotoxicity. This effect of Jagamcho-tang is mimicked by treatment by pretreatment of SNP($100{\mu}M$), an exogenous NO donor. NG-monomethyl-L-arginine($N^{G}MMA$), a specific inhibitor of nitric oxide synthase (NOS), significantly blocks the protective effects of Jagamcho-tang on cell toxicity by ischemia. In addition, lipopolysaccharide(LPS) and phorhol 12 myristate 13-acetate(PMA) treatment for 72h in C6 glial cells markedly induce NO, but treatment of the cells with the water extracts of Jagamcho-tang decrease nitrite formation in a dose dependent manner. In addition, LPS and PMA treatment for 72h induce severe cell death and LDH release into medium in C6 glial cells. However treatment of the cells with the water extracts of Jagamcho-tang dose not induce significant changes compare to control cells. Furthermore, the protective effects of the water extracts of Jagamcho-tang is mimicked by treatment of $N^{G}MMA$. Taken together, I suggest that the protective effects of the water extracts of Jagamcho-tang against ischemic brain damages may be mediated by regulation of iNOS during ischemic condition.
이규호 ( Kyu Ho Lee ),김태호 ( Tae Ho Kim ),정영준 ( Young Seok Kim ),김영석 ( Young Joon Jung ),나영훈 ( Young Hoon Na ),류봉기 ( Bong Ki Ryu ) 대한금속재료학회 ( 구 대한금속학회 ) 2008 ELECTRONIC MATERIALS LETTERS Vol.4 No.1
This paper presents results and observations obtained from a study of crystallization behavior in Li2O-Al2O3- SiO2 (LAS) glass powders. Variable kinetic parameters have been obtained in order to investigate the crystallization behavior by using non-isothermal differential thermal analysis. LAS glass powders including B2O3 which induce a low firing process have been melted and crushed to obtain coarse and fine glass powder. In case of coarse particles with 88 μm, the crystallization have been generated at 649~699°C, the crystallization of fine particles with 44 μm have been observed at 640~684°C according to heating rate. In order to obtain the activation energy of crystallization (EC) and Avramic constant (n), Kissinger and Ozawa equations have been used. The average activation energies of crystallization were [44 μm=48.09±1 kcal/ mol, 88 ?m = 53.02±1 kcal/mol] Avramic constant (n) were [44 μm=1.3, 88 ?m=1.6] in Li2O-Al2O3-SiO2 (LAS) glass. Finally, we have observed the surface and bulk crystallization of β-spodumene were occurred simultaneously in this glass powder by using SEM (Scanning Electron Microscopy) and XRD (X-ray diffraction).
生脈散이 대동맥 평활근 세포에서 NO 생성에 미치는 영향
허윤,나영훈,한상혁,장호연,임준모,이인,전희준,류지용,문병순 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.5
Nitric oxide(NO) play an important role in normal and pathophysiologcal cells including as a messenger molecule, neurotransmitter, microbiocidal agent, or dilator of blood vessels and arteriosclerosis, respectively. To understand the mechanism of NO production and effect of Saengmaeg-san extract on NO production in cultured vascular smooth muscle cells(VSMC), firstly, VSMC was isolated from aorta and cultured. Cultured primary cells was identified as VSMC with anti- α smooth muscle actin antibody. High amounts of NO was produced in cultured VSMC treated with IFN-γ and TNF-α as time- and dose-dependent manner. TNF-α was a more efficient stimulator than IFN-γ on the respect to NO production in cultured VSMC. iNOS protein was detected within 3 hr and the detected iNOS was increased up to 12 hr as time-dependent manner. However, accumulated NO in activated VSMC was not detected within 3 hr. NO production in activated VSMC showed the dose- and time-dependent manner, and was increased up to 72 hr. The activated VSMC produced high amounts of NO (about 70 uM) and showed cytotoxicity. The cytotoxicity was apparently responsible to high amounts of NO produced in activated VSMC. Saengmaeg-san extract decreased the accumulation of NO in IFN-γ plus TNF-α or TNF-α treated aortic VSMC. Saengmaeg-san extract suppressed the effect of TNF-α on NO production. TNF-α functions signal molecules for apoptosis in some cells. As NO deeply involved in the development of arteriosclerosis and dilation of blood vessels and NO induced apoptosis in cultured VSMC, one have thought that drugs or chemicals modulating the NO production in VSMC could be used for preventing and treating the arteriosderosis. Therefor, The results strongly suggested that Saengmaeg-san have a potential capacity for preventing and treating the disease of cardiovascular system including arteriosclerosis.