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딸기 조직배양 시 BA (benzyladenine) 처리에 따른 변이 발생 및 변이 연속성 검정
김혜진,최미자,이종남,서종택,김기덕,김율호,홍수영,김수정,손황배,남정환,Kim, Hye Jin,Choi, Mi Ja,Lee, Jong Nam,Suh, Jong Taek,Kim, Ki Deog,Kim, Yul Ho,Hong, Su Young,Kim, Su Jeong,Sohn, Hwang Bae,Nam, Jeong Hwan 한국식물생명공학회 2020 식물생명공학회지 Vol.47 No.1
본 실험은 딸기 조직배양 시 BA 처리에 따른 변이 발생 및 변이 연속성을 확인하고자 실시하였다. 본 실험에 사용된 공시 품종은 '고하'이며, 본 실험에 사용한 BA 농도는 0.0, 0.5, 1.0, 2.0 mg·L<sup>-1</sup>로 처리하였다. 변이는 형태적, 유전적 검정을 실시하였으며, 변이 연속성 검정은 3년간 실시하였다. BA 처리 시 형태적 변이는 10.5 ~ 20.0%로 매우 높게 나타났으나, 유전적 변이는 재배 1년차에 7.0 ~ 15.0%, 재배 2년차에는 1.8 ~ 10.0%, 재배 3년차에는 5.0%로 재배연수가 길어짐에 따라 유전적 변이 발생율이 낮아졌다. 뿐만 아니라 재배1년차와 2년차에는 BA 1.0 mg·L<sup>-1</sup>과 BA 2.0 mg·L<sup>-1</sup>에서 유전적 변이가 발생한 반면, 재배 3년차에는 BA 2.0 mg·L<sup>-1</sup>에서만 유전적 변이가 발생하였다. 따라서 딸기 조직배양묘의 증식을 위해서 BA는 1.0 mg·L<sup>-1</sup>미만으로 처리하고, 반드시 변이 검정 후 보급하는 것이 바람직하다고 판단되었다. This experiment study aimed to identify the continuous genetic variation caused by benzyladenine (BA) treatment in strawberry tissue culture. The 'Goha' cultivar was used and treated with different concentrations of BA (0.0, 0.5, 1.0, 2.0 mg·L<sup>-1</sup>). Morphological and genetic variation tests were performed, and genetic continuity tests were performed for three years. The morphological variation induced by BA was distinctively high (10.5 ~ 20.0%) and the genetic variation was 7.0 ~ 15.0%, 1.8 ~ 10.0%, and 5.0% in the first, second, and third year of cultivation, respectively. The rate of genetic variation decreased with increasing cultivation years. In addition, genetic variation caused by BA 1.0 mg·L<sup>-1</sup> and BA 2.0 mg·L<sup>-1</sup> occurred in the first and second years of cultivation, whereas only BA 2.0 mg·L<sup>-1</sup> caused genetic variation in the third year of cultivation. Therefore, a concentration of less than 1.0 mg·L<sup>-1</sup> BA was used for the propagation of strawberry tissue culture plants, and it was necessary to identify their variation.
Hye Jin Lee(이혜진),Seong Jong Yoon(윤성종),Young Se Hyun(현영세),Hye Jin Kim(김혜진),Sung-Il Hwang(황성일),Joo-Seung Bae(배주승),Ki Wha Chung(정기화) 한국생명과학회 2013 생명과학회지 Vol.23 No.9
꽃게(Portunus trituberculatus)는 세계적으로 넓게 분포하는 갑각류로 모래나 돌멩이가 있는 해저에 서식한다. 본 연구는 서해의 4개 지점(영광, 태안, 소래, 연평도)에서 채집된 P. trituberculatus 281 개체에 대해 10 종류 microsatellite 좌위의 유전적 다형성을 조사하였다. 좌위당 대립유전자 수는 50-129개로 평균 69.5개였으며, 관측 및 예상 이형접합도는 각각 0.111-1.000 및 0.609-0.979 범위에 있었다. 좌위별 근친계수((Fis)는 -0.0207에서 0.8175 범위였다. 유전적 분화도(Fst)는 0.05보다 낮게 나타났는데, 이것은 4 꽃게 간의 유전적 분화(genetic differentiation)가 매우 낮은 이루어진 것으로 추정하게 한다. UPGMA을 이용한 계통도 작성에서도 4 그룹 간의 유전적 거리는 매우 가깝다는 결과를 얻을 수 있었다. 매우 높은 다형성과 집단간의 낮은 유전적 분화는 서해안의 꽃게 집단은 활발한 유전적 흐름(gene flow)이 일어나며, 그룹간 지리적 경계가 없음을 제시한다. The swimming crab, Portunus trituberculatus, inhabits seafloor habitats containing sand or pebbles and is widely distributed throughout the world. The present study investigated genetic polymorphisms of 10 microsatellites in 281 samples of P. trituberculatus collected from four locations along the coastal water of the Korean side of the Yellow Sea (Yeonggwang, Taean, Sorea, and Yeonpyeong-do Island). The number of alleles per locus ranged from 50 to 129, with a mean of 69.5. The observed and expected hetrozygosity varied from 0.111 to 1.000 and from 0.609 to 0.979, respectively. The inbreeding coefficients (Fis) varied among the loci from -0.0207 to 0.8175. The genetic differentiation (Fst) was less than 0.05 (range 0.0020-0.0124). Therefore, the four groups of P. trituberculatus appeared to exhibit little genetic differentiation. The lack of differentiation was confirmed in a phylogenetic tree constructed by the unweighted pair group method with the arithmetic average (UPGMA). The hypervariation between the populations and the lack of genetic differentiation may reflect active gene flow among the Yellow Sea populations and the absence of geographical boundaries. The highly polymorphic microsatellite loci will be useful for molecular and phylogenetic studies, as well as stock management, of swimming crab, which is an important fishery resource.
딸기 조직배양묘의 생물반응기 배양 시 Flurprimidol 처리농도에 따른 기내 생장억제 및 순화율 향상
김혜진(Hye Jin Kim),이종남(Jong Nam Lee),김기덕(Ki Deog Kim),권기범(Gi Bum Kwon),유동림(Dong Lim Yoo),임학태(Hak Tae Lim),용영록(Young Rok Yeoung) 한국원예학회 2015 원예과학기술지 Vol.33 No.4
This study was conducted to improve the acclimatization rate of in vitro strawberry plantlets through bioreactor culture using the growth retardant flurprimidol. Different concentrations [0 (Control), 0.1, 0.5, 1.0, and 2.0 ㎎·L<SUP>-1</SUP>] of flurprimidol were added during bioreactor culture. After six weeks of treatments, various growth characteristics were investigated and in vitro plantlets were acclimated in the greenhouse. The growth rate of treated plantlets was much lower than that of control, and as the treatment concentration increased, the growth rate was much decreased. Shoots of plantlets treated with flurprimidol were shorter (2.2-3.7 ㎝) than those of control (7.9 ㎝). The number of roots per treated plant was around 11.6-34.2, compared with 51.8 in the control. Root length was also lower (0.88-3.08 ㎝) than control (4.36 ㎝). However, the number of new shoots and leaves increased in all treatments except for 2.0 ㎎·L<SUP>-1</SUP> concentration. The root was partially decayed in 1.0 ㎎·L<SUP>-1</SUP> concentration and was completely decayed in 2.0 ㎎·L<SUP>-1</SUP>. The survival rate in 0.1 ㎎·L-1 and 0.5 ㎎·L<SUP>-1</SUP> concentrations was 100% and 23.3% respectively. After four weeks of acclimatization, the plantlets restarted growth, and growth characteristics of shoots and roots recovered to the levels of control, except for fresh weight. Based on our results, a concentration of 0.1 ㎎·L<SUP>-1</SUP> flurprimidol is appropriate for improvement of acclimatization rate of in vitro strawberry plantlets in bioreactor culture.
사계성 딸기 ‘고하’ 조직배양묘의 대량증식 시 생물반응기 내 공기주입량에 따른 생육 특성
김혜진(Hye Jin Kim),이종남(Jong Nam Lee),김기덕(Ki Deog Kim),임주성(Ju Sung Im),임학태(Hak Tae Lim),용영록(Young Rok Yeoung) 한국원예학회 2012 원예과학기술지 Vol.30 No.4
This study was conducted to determine the optimal aeration rate for mass propagation of ever-bearing strawberry by bioreactor culture. The aeration rate was treated in four levels: 0.1 vvm (air volume/medium volume/min), 0.2 vvm, 0.3 vvm, and 0.4 vvm. In 0.2 vvm conditions, shoot length was the longest at 9.03 ㎝ in bioreactor culture, leave numbers were 40.4 ea and fresh weight was 6,106 ㎎. Plant growth rate at 0.2 vvm condition was faster than other treatments. In the aeration condition, 0.2 vvm was most effective to increase aerial part growth and to decrease medium consumption. As the culture periods increased, the fresh weight also increased rapidly. After six weeks of cultivation, shoots were emerged with 10.4 ea per plantlet, resulting in developing a complete plant. As a result, the bioreactor culture system for mass propagation of strawberry is required to continuously supply the air by 0.2 vvm speed and cultivate at least for six weeks.