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金海俊(Hae-Jun Kim),元載善(Jae-Sun Won),朴宰煜(Jae-Wook Park),徐喆植(Cheol-Sik Seo),李成熙(Seung-Hee Lee),沈光列(Kwang-Yeal Sim),金鍾海(Jong-Hae Kim),金東熙(Dong-Hee Kim) 대한전기학회 2007 전기학회논문지 Vol.56 No.12
A novel design technology for a high frequency electronic ballast for metal-halide discharge lamps is proposed. A PFC technique is adopted to get unit power factor and output frequency alteration technique is adopted to avoid acoustic resonance. For characteristics evaluation, the designed electronic ballast is presented using normalized parameter. To reduce losses of the ballast, ZVS control technique is adopted and the maximum flux density of magnetic core for inductor should be kept lower. The electronic ballast for 250[W] metal-halide discharge lamp is implemented and 96[%] efficiency and low conducted EMI level are accomplished.
김세라,김태환,이해준,오헌,조성기,오기석,박인철,손창호,김성호,Kim, Se-Ra,Kim, Tae-Hwan,Lee, Hae-Jun,Oh, Heon,Cho, Sung-Ki,Oh, Ki-Seok,Park, In-Chul,Son, Chang-Ho,Kim, Sung-Ho 한국임상수의학회 2003 한국임상수의학회지 Vol.20 No.3
Cytogenetic and hematological analysis was performed in peripheral blood from the Korean native goat bred in the nuclear power plant (Wolsong and Uljin) and control area. The frequency of micronuclei (MN) in peripheral blood lymphocytes from goat was used as a biomarker of radiobiological effects resulting from exposure to environmental radiation. An estimated dose of radiation was calculated by best fitting linear-quadratic model based on the radiation-induced MN data over the range from 0 to 4 Gy from the goat lymphocytes with in vitro irradiation. MN rates in goats from the Wolsong and Uljin nuclear power plant, and control area were 9.60/1000, 6.83/1000 and 9.88/1000, respectively. There were no significant differences in MN frequencies and hematological values in goats between nuclear power plant and control area. High level of platelet in the goat from Uljin nuclear power plant was observed, which seemed to be related to the goat management.
연구논문 : 실시간 대화형 화산재 확산 예측 시스템에 의한 화산재 확산 예측
김해동 ( Hae Dong Kim ),이전희 ( Jun Hee Lee ) 한국환경과학회 2014 한국환경과학회지 Vol.23 No.12
Analyzing the observational data of volcanic activities around the northern part of Korean peninsula, the odds of volcano eruption increases continuously. For example, the cumulative seismic moment and frequence observed near Mt. Baekdu show a sudden increased values. In this study, predicting the diffusion of volcanic ash for two cases were carried out by using interactive realtime simulator, which was developed during last 2 years as a research and development project. The first case is Sakurajima volcano (VEI=3) erupted in August 2013. The second case is assumed as the volcanic eruption at Mt. Baekdu (VEI=7) under landing circumstance of typhoon Maemi (August 2003) in Korean peninsula. The synoptic condition and ash diffusion for the two cases were simulated by WRF(Weather Research and Forecast) model and Lagrangian dispersion model, respectively. Comparing the simulated result of the first case (i.e., Sakurajima volcano) with satellite image, the diffusion pattern show acceptable result. The interactive realtime simulator can be available to support decision making under volcanic disaster around East Asia by predicting several days of ash dispersion within several minutes with ordinary desktop personal computer.
Production of Poly-3-hydroxybutyrate from Xylose by Bacillus megaterium J-65
Jun Hong-Ki(전홍기),Young-Hi Jin(진영희),Hae Nam Kim(김해남),Yun-Tae Kim(김윤태),Sam Woong Kim(김삼웅),Hyung Suk Baik(백형석) 한국생명과학회 2008 생명과학회지 Vol.18 No.12
본 연구는 생분해성 플라스틱인 poly-β-hydroxybutyrate (PHB)의 생산단가를 낮추기 위한 노력으로 저가의 기질로부터 PHB 대량생산을 위한 기초자료를 얻는데 그 목적을 두었다. Hemicellulose hydrolysate는 지구상에 풍부하게 존재하는 저가의 waste by-product로서 xylose가 많이 포함되어 있다. 본 연구에서는 xylose로부터 PHB를 생산할 수 있는 균주를 토양에서 분리하여, 분류학적 위치를 밝히고, 균체 생육 최적 조건, PHB 생산을 위한 최적 발효 배양 조건, PHB의 구조 확인 등을 검토 하였으며, 그 결과는 다음과 같다. 토양으로부터 분리한 균주 J-65는 형태학적, 배양적, 생화학적 및 partial 16S rRNA sequence에 근거하여 Bacillus megaterium로 동정하였다. B. megaterium J-65의 균체 생육 최적 조건은 온도 37℃, 초발 pH 8.0이었으며 2% xylose, 0.25% (NH₄)₂SO₄, 0.3% Na₂HPO₄ㆍ12H₂O, 0.1% KH₂PO₄였다. PHB 축적에 영향을 미치는 요인을 검토하기 위해 균체생육 최적배지에서 37℃, 24시간 1차 배양한 후, 균체를 회수하여 각종 영양분이 결핍된 배지에 2차 배양을 실시한 결과 B. megaterium J-65는 균형생육조건(balanced-growth condition)에서 PHB를 합성하는 균주로 나타났다. PHB보다 물성이 향상된 PHB/HV 공중합체를 생산하기 위하여 보조기질로 propionic acid를 첨가하였을 때, 0.1% propionic acid 농도에서 HV ㏖%가 14%인 PHB/HV 공중합체가 합성되었다. 5 l 용량의 발효조에 B. megaterium J-65를 회분배양하였을 때 배양 21시간에 건조균체량 10 g/l, PHB 3.5 g/l를 얻을 수 있었고, 유가배양을 실시한 결과 배양 48시간에 건조균체량 26.52 g/l, PHB 9.28 g/l를 얻을 수 있었다. 생산된 PHB를 alkaline solution 처리와 chloroform을 이용한 유기용매 추출법을 이용하여 추출ㆍ정제한 후 Gas Chromatography로 정제를 확인하고 300㎒ 1H-NMR을 실시한 결과 3-hydroxybutyrate의 homopolymer임을 확인하였다. A microorganism capable of producing high level of poly-3-hydoxybutyrate (PHB) from xylose was isolated from soil. The isolated strain J-65 was identified as Bacillus megaterium based on the morphological, biochemical and molecular biological characteristics. The optimum temperature and pH for the growth of B. megaterium J-65 were 37℃ and 8.0, respectively. The optimum medium composition for the cell growth was 2% xylose, 0.25% (NH₄)₂SO₄, 0.3% Na₂HPO₄ㆍ12H₂O, and 0.1% KH₂PO₄. The optimum condition for PHB accumulation was same to the optimum condition for cell growth. Copolymer of β-hydroxybutyric and β-hydroxyvaleric acid was produced when propionic acid was added to shake flasks containing 20 g/l of xylose. Fermenter culture was carried out to produce the high concentration of PHB. In batch culture, cell mass was 9.82 g/l and PHB content was 35% of dry cell weight. PHB produced by B. megaterium J-65 was identified as homopolymer of 3-hydoxybutyric acid by GC and NMR.
김정표 ( Jeong Pyo Kim ),김성훈 ( Seong Hun Kim ),이해규 ( Hae Giu Lee ),성미숙 ( Mi Sook Sung ),김영실 ( Young Sil Kim ),김형민 ( Hyoung Min Kim ),민준기 ( Jun Ki Min ) 대한류마티스학회 2003 대한류마티스학회지 Vol.10 No.1
Sarcoidosis is a multisystemic granulomatous disease of unknown cause. Lung, skin, eye, liver and lymph nodes are commonly affected. But the nodular type of muscular sarcoidosis is rare. The nodular type can be presented with a soft tissue mass that may be confused with a tumor. Magnetic resonance imaging and muscle biopsy are useful methods for early diagnosis. Optimal management of nodular sarcoidosis is not well known, but surgical excision or corticosteroid may be considered as an initial management. We report a case of nodular muscular sarcoidosis as an initial manifestation of sarcoidosis in a 56-year-old woman who was treated with corticosteroid therapy after surgical excision.