Analysis of BRIP1 Variants among Korean Patients with BRCA1/2 Mutation-Negative High-Risk Breast Cancer

김해영,조대연,최두호,정지혜,신인경,박원,허승재,남석진,이정언,길원호,김석원 대한암학회 2016 Cancer Research and Treatment Vol.48 No.3

Purpose The aim of the current study is to assess the spectrum of genetic variation in the BRIP1 gene among Korean high-risk breast cancer patients who tested negative for the BRCA1/2 mutation. Materials and Methods Overall, 235 Korean patients with BRCA1/2mutation–negative high-risk breast cancer were screened for BRIP1mutations. The entire BRIP1 gene was analyzed using fluorescent-conformation sensitive gel electrophoresis. In silico analysis of BRIP1 variants was performed using PolyPhen-2 and SIFT. Results A total of 20 sequence alterations including 12 exonic and eight intronic variants were found. Among the 12 exonic variants, 10 were missense and two were silent mutations. No protein- truncating mutation was found among the tested patients. Among the 10 missense variants, four (p.L263F, p.L340F, p.L474P, and p.R848H) were predicted to be pathogenic by both PolyPhen-2 and SIFT, and these variants were found in five patients. Of the four missense variants, p.L263F, p.L474P, and p.R848H localize to regions between the helicase motifs, while p.L340F resides in an iron-sulfur domain of BRIP1. Conclusion No protein-truncating mutation in BRIP1 was found among the tested patients. The contribution of BRIP1 variants is thought to be minor in Korean non-BRCA1/2 high-risk breast cancer.

엄마가 옛날 이야기 해줄까?

김해영 샘터사 2003 월간 샘터 Vol.34 No.10

귀한 자식일수록

김해영 샘터사 2004 월간 샘터 Vol.407 No.-

“나노사회의 ‘파편화’(fragmentation) 현상과 공동체 약화에 관한 목회상담학적 연구

김해영 한국실천신학회 2023 신학과 실천 Vol.- No.84

Kim Nan-do, a professor at Seoul National University, studies and presents Korean trends every year. This study aims to deal with the modern meaning of the word "nano-society" that he chose as one of the key keywords in 22 and the pastoral counseling perspective on it. What members of the nano-society are showing as figures in social change are families living alone, personalization of consumption patterns, closed hobbies, and isolation due to closed information sharing. Among these social characteristics, what this study sees with an uneasy perspective are the emotional problems experienced by the individuals who make up the nano-society and the resulting high suicide rate. What's more worrisome is the idea that we seem to be experiencing a society where even these fearful consequences that members of society are experiencing can be dismissed as personal problems. With this sense of crisis, this study analyzes the figurative meaning of nano-society he refers to and examines the cause from Charles Taylor's point of view. Using Taylor's terminology, the study believes that nanosocialism, which Professor Kim Nan-do refers to, is eventually in a state of "fragmentation" in which communities are divided and individuals experience a severe sense of extreme isolation. As a pastoral counseling response, this study suggests the meaning of Donald Capps' "hope" based on Christian tradition as an alternative. He says pastors should appear as "representatives of hope" for people in need in the context of counseling. This study aims to examine the implications. The study considers the answers to what hope he says means and what function it can play in nano-society. Furthermore, we will deal with what the specific application point of hope that Capps says can be so that hope does not simply become a groundless cry that makes us see the situation ideally. From that point of view, this study specifically examines the meanings of "trust," "patience," and "humility" presented as three assistants of hope and presents them as pastoral counseling approaches of nano-society.

지속성 진통작용 물질인 Capsaicinoid 의 흰쥐 척수내 Substance P 함량 및 생합성에 미치는 영향

김해영,손여원,이상섭,이승기 ( Hai Young Kim,Yeo Won Sohn,Sang Sup Lee,Seung Ki Lee,Seung Ki Lee ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.2

We aimed in this study to evaluate the effects of capsaicinoids on the cellular concentration of substance P(SP) and substance P-mRNA in rat spinal cord. This study was based on the fact that longlasting analgesic, capsaicin caused depletion of SP from primary afferent neurons in dorsal root ganglia. After administration of 6-paradol (80, 500, 1000 ㎎/㎏) or capsaicin (80 ㎎/㎏) in rats, the cellular concentration of SP and its mRNA in spinal cord were analyzed by radioimmunoassay and slot blot hybridization analysis respectively. 6-Paradol, like capsaicin, reduced in dose-dependent manner the content of SP in rat spinal cord. Additionaliv, SP-mRNA concentration was also reduced to 61% of the control level at 3 h after capsaicin administration and was restored to the control level after 1 day. In 6-paradol treated group, substance P-mRNA concentration was not detectably reduced at 3 hours but significantly reduced to 49% of the control level at 1 day after 6-paradol treatment. These results support for the previous notion suggesting that the action mechanism of capsaicinoid analgesia may be primarily based on the depletion of SP from primary afferent neurons. These results also suggest that capsaicinoid analgesics may play a role at the transcription level of SP synthesis in rat spinal neurons.

성인교육에서 자기주도학습 준비도와 성격유형 및 귀인양식이 학업성취도에 미치는 영향

김해영 충남대학교 교육연구소 2000 교육연구논총 Vol.21 No.1

孝治思想의 淵源과 韓國的 展開

김해영 한국효학회 2014 효학연구 Vol.0 No.19

이 논문은 孝治思想의 淵源과 韓國的 展開過程을 고찰한 글이다. 주지하듯 孝는 儒家思想의 핵심적인 개념 가운 데 하나다. 孝가 윤리의 기반이자 출발인 것이다. 요컨대 부모의 慈와 자식의 孝가 올바르게 구현될 때, 父子間 의 일체의식이 이루어지며, 형의 友愛와 동생의 恭遜, 윗사람의 理解와 아랫사람의 順從이 올바르게 구현될 때, 兄弟間의 질서뿐만이 아닌 사회의 질서의식이 확립되며, 남편의 올바름과 아내의 德性이 올바르게 구현될 때, 부부간의 역할분담이 調和를 이루며, 지도자의 사랑과 아랫사람의 忠誠이 올바르게 구현될 때, 정의사회도 이룩 될 수 있다고 보는 것이다. 그런 의미에서, 孔孟이 주장하는 孝와 漢代에서의 孝治, 우리의 檀君과 三國時代의 孝治, 高麗와 正祖以前까지 의 孝治를 고찰하면서 과연 어떤 同異문제가 존재하는지에 대해 고찰하였다. 이를테면 孔子의 “그 사람됨이 孝 誠스럽고 友愛가 있으면서 윗사람 犯하기를 좋아하는 사람이 적으니, 윗사람 犯하길 좋아하지 않으면서 亂을 일 으키기를 좋아하는 자는 있지 않다. 군자는 根本에 힘쓰니, 근본이 서면 道가 생기니 孝誠과 友愛는 仁을 行하는 根本”이라는 논리와 우리의 孝治思想이 어떻게 같고 다른지에 관해 살핀 것이다. 결과적으로 孝治思想의 韓國的 展開와 孔孟이 주장하는 그것이 별도의 문제가 아니었음을 확인하였다. 그러나 孝治는 운용하는 자에 따라 양날의 칼로 작용할 수 있다는 점에서 省察이 필요하다 하겠다. 이는 인간 상 호관계의 덕목에 대하여 거듭되는 이야기이지만 禮記 에서는 부모의 慈와 자녀의 孝, 형의 良과 동생의 弟, 윗사람의 惠와 아랫사람의 順, 남편의 義와 부인의 德, 군주의 仁과 신하의 忠을 연계시키고 있고, 齊나라 景公 의 政治에 대한 질문에, 孔子는 “君君, 臣臣, 父父, 子子”로 답한 것에서 해답을 찾을 수 있다. 孝治가 다름이 아 닌 父子倫理 관계에서 부모가 먼저 자식을 慈愛롭게 대할 때, 자식 또한 부모에게 孝誠을 다한다는 이른바 互惠 倫理에서 출발한다는 점에 유의할 필요가 있다 하겠다. This paper aims to investigate the origin of thoughts on rule by means of filial piety and its development process in Korea. Filial piety(孝, “Hyo”) is one of essential concepts in Confucian thoughts. That is, filial piety is the foundation for ethics as well as a start. Confucianists believe that when affection of parents and filial piety of their children are demonstrated in a righteous manner, a sense of unity between father and son shall be established. When fraternity and brotherly love of elder brotherhood and politeness of younger brotherhood, and understanding as those above and obedience as those below are rightly materialized, the orders between brothers as well as a sense of order in society shall be firmly established. And that when rightness of husbandship and virtue of wifeliness are rightly visualized, the role sharing between husband and wife will be harmonized, and that when the love of the leaders who stay true to their role and mission and loyalty of those below are rightly realized, the just society can be established. In that sense, this research looked into the filial piety asserted by Confucius-Mencius; the ruling with filial piety in Han Dynasty(漢代); the governance by filial piety during Dangun(檀君) and Three-kingdom Era(三國時 代); and the governance by filial piety during Goryeo(高麗) until the reign of King Jeong-Jo(正祖), and closely examined if there existed some similarities and differences among them. Specifically, the study tried to figure out how Thoughts on Rule by means of Filial Piety(孝治思想, “Hyochi Sasang”) in Korea are different from Confucian’s logic such as “There are few who, being filial and fraternal, are fond of offending against their superiors. There have been none, who, not liking to offend against their superiors, have been fond of stirring up confusion. The superior man bends his attention to what is radical. That being established, all practical courses naturally grow up. Filial piety and fraternal submission, are they not the root of all benevolent actions? In consequence, this research confirmed that the thoughts on the rule by means of filial piety and its development process in Korea and those that asserted by Confucius and Mencius are not separate issues. However, it shall be required self-reflection in view of the fact that the ruling with filial piety may act like a double-edged sword depending upon the man who uses it. This is a repeated remark on the virtue concerned with interpersonal relationship, meanwhile, in the Book Of Rites(“Yegi” 禮記), close connections had been established between the virtues such as affection of parents and filial piety of children; good and kind-hearted of elder brother and respect and politeness of younger brother; generosity as those above and obedience as those below; righteousness of husband and virtue of wife; benevolence of ruler and loyalty of subjects. Also the study found a solution from the Confucius’ reply to the question of the duke Jing, of Qi(齊景公), to wit, “There is government, when the prince is prince, and the minister is minister; when the father is father, and the son is son. (君君,臣臣,父 父,子子, “Gun-gun, Sin-sin, Bu-bu, Ja-ja”). It is necessary to note the key point that the rule with filial piety begins with, no other than, the ethics between father and son in that parents firstly care their children with love and grace, so then the children will pay their filial devotion to their parents in turn, namely, the ethics based on mutually beneficial relationship(互惠倫理, “Hohye Yunri”)

5'-Untranslated Region 에 존재하는 Iron Responsive Element 에 의한 Ferritin 합성조절

김해영,정인식,이중림 ( Hae Yeong Kim,In Sik Chung,Jung Lim Lee ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.3

The expression of ferritin involved in iron metabolism is regulated at the translational level by the interaction of iron regulatory protein with iron-responsive element(IRE) in the 5`-untranslated region of ferritin transcript. To identify the role of structural element utilized for translational regulation of ferritin, we studied the effects of mutations in the ferritin IRE by measuring IRP binding activity and translational activity. Our data suggest that the cytosine at bulged position of IRE within ferritin is important for the formation of RNA secondary structure involved in translational regulation.

당선소감

김해영 문예운동사 2007 수필시대 Vol.2 No.-

Synthesis of Flame Retardants Based on Cyclotriphosphazene

김해영,지영존,신재섭 한국고분자학회 2004 한국고분자학회 학술대회 연구논문 초록집 Vol.2004 No.04