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당느릅나무로부터 Sesquiterpene o - Naphthoquinone 류 화합물 , Mansonone E , F 및 H 의 분리와 구조 결정
김종평(Jong Pyung Kim),김원곤(Won Gon Kim),박종희(Jong Hee Park),정진(Jin Jung),유익동(Ick Dong Yoo),(Hiroyuki Koshino) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.1
Three sesquiterpene ortho-naphthoquinones were isolated from the methanolic extract of root bark of Ulmus davidiana Planch whose stem and root bark have been used as an oriental medicine for the treatment of edema, mastitis, gastric cancer and inflammation. The structures of these compounds were established on the basis of spectral data obtained from UV-vis, IR, HR-EIMS and NMR spectrometry, including the pulse field gradient (PFG)-HMQC and HMBC techniques. Their structures were determined as 2,3-dihydro-3,6,9-trimethylnaphtho(1,8-b,c)pyran-7,8-dione, 3,6,9-trimethylnaphtho(1,8-b,c)pyran-7,8-dione and 2,3-dihydro-4-hedroxy-3,6, 9-trimethylnaphtho(1,8-b,c)pyran-7,8-dione, which were identified as mansonones E. F and H, respectively. These compounds have originally been isolated from Mansonia altissima Chev, but have never been isolated from Ulmus davidiana Planch. Especially, mansonone H was isolated for the first time from Ulmaceae. The mismatched carbon chemical shifts of mansonones E and F in the reported literature were corrected by the aid of the PFG-HMBC spectral data.
강학수,김종평 한국응용생명화학회 2019 Applied Biological Chemistry (Appl Biol Chem) Vol.62 No.-
The organic extract of cultured Aspergillus terreus displayed scavenging activity against ABTS•+ and DPPH free radicals, and protective activity against glutamate-induced excitotoxicity in N18-RE-105 neuroblastoma-retina hybrid cells. Bioassay-guided fractionation of the active organic extract led to the isolation of total six butenolide derivatives, including one new metabolite, named butyroscavin (1), and five previously described metabolites, butyrolactones I (2), II (3), III (4), and VII (5), and aspernolide E (6). The planar structure of butyroscavin (1) was determined by the analysis of spectroscopic data including ESIMS (electrospray ionization mass spectrometry), and 1D and 2D NMR (nuclear magnetic resonance). The absolute configuration of butyroscavin (1) was assigned by comparison of the specific rotation with those of known compounds that share the same chiral carbon. All isolated compounds were active in the radical scavenging assay, whereas only butyrolactones I (2) and VII (5) exhibited protective activity against the glutamate-induced excitotoxicity with the EC50 of 130.1 and 91.9 μM, respectively.
정진,김종평,한창균 한국농화학회 1991 Applied Biological Chemistry (Appl Biol Chem) Vol.34 No.2
The uptake of Mn^(+2), a metal cofactor Mn-SOD, by rice seedings resulted in not only a substantial increase in SOD activity in leaf tissues of the plants, but also a significant enhancement of their cold tolerance : the relative extent of the cold tolerance appeared to accord with relative level of the SOD activity. In contrast, Fe^(+3). Cu^(+2) and Zn^(+2). which are the cofactors of Fe-SOD and Cu/Zn-SOD, were found to be ineffective for increasing the SOD activity as well as for improving the chilling-resistant capacity of the plants. The results suggest that Mn-SOD, which is most likely induced by its substrate(superoxide) and activated by the presence of Mn^(+2) at high level, is the enzyme acting as an active component of the defense system against low temperature stress in rice plants. In addition, the application of abscisic acid which has been know to protect to some extent certain plants from chilling injury brought about an increase in SOD activity in rice tissues, providing another affirmative information for the crucial role of SOD under the circumstance of cold stress in plants.
식물의 냉해에 대한 생체방어기구로서 항산소성 효소의 유도 : (II) $Mn^{+2}$이온에 의한 세포내 SOD의 활성화와 벼 유묘의 내냉성 향상
한창균,김종평,정진,Hahn, Chang-Kyun,Kim, Jong-Pyung,Jung, Jin 한국응용생명화학회 1991 Applied Biological Chemistry (Appl Biol Chem) Vol.34 No.2
벼 유묘에 의 한 $Mn^{+2}$(Mn-SOD의 cofactor)의 흡수는 유묘조직중 SOD 활성을 증가시킴과 아울러 유묘의 냉해저항성을 현저히 향상시키는 결과를 보였으며, SOD 활성 증가정도와 냉해 저 항성 향상정도간에는 정의 상관관계가 있었다. 이에 반하여, Fe-SOD와 Cu/Zn-SOD의 cofactor들인 $Fe^{+3},\;Cu^{+2},$ 및 $Zn^{+2}$의 흡수는 조직내 SOD활성이나 식물의 냉해저항성에 어떤 유의성 있는 영향도 미치지 않았다. 이러한 결과들이 시사하는 바는 아마도 superoxide에 의해 유도되고 $Mn^{+2}$의 존재에 의해 활성화된 Mn-SOD가 (최소한 벼의 경우에는) 저온 스트레스에 대항하는 생체방어 시스템의 중요한 子성분일 것이라는 점이다. 어느정도의 냉해억제효과가 있다고 인정된 Abscisic acid의 처리도 벼 유묘조직의 SOD 활성을 증가시켰다. 이 관찰결과도 식물의 냉해 유발상황 하에서 세포내 SOD가 담당하는 중요한 생체방어 역할을 부각시키는 또 하나의 정보를 제공한 것이다. The uptake of $Mn^{+2}$, a metal cofactor Mn-SOD, by rice seedings resulted in not only a substantial increase in SOD activity in leaf tissues of the plants, but also a significant enhancement of their cold tolerance : the relative extent of the cold tolerance appeared to accord with relative level of the SOD activity. In contrast, $Fe^{+3},\;Cu^{+2}$ and $Zn^{+2}$, which are the cofactors of Fe-SOD and Cu/Zn-SOD, were found to be ineffective for increasing the SOD activity as well as for improving the chilling-resistant capacity of the plants. The results suggest that Mn-SOD, which is most likely induced by its substrate(superoxide) and activated by the presence of $Mn^{+2}$a at high level, is the enzyme acting as an active component of the defense system against low temperature stress in rice plants. In addition, the application of abscisic acid which has been know to protect to some extent certain plants from chilling injury brought about an increase in SOD activity in rice tissues, providing another affirmative information for the crucial role of SOD under the circumstance of cold stress in plants.
정진,김종평 한국농화학회 1990 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.2
The level of superoxide radical(O^-₂) and the activities of antioxygenic enzymes in leaves were measured with plants exposed to the stress--inducing environmental factors such as low temperature, high temperature, and high intensity light. The time-courses of O₂ level change in plants which had been subjected to various environmental treatments indicated that O^-₂was indeed involved in the environmental stress. Since ocher active oxygens are produced through secondary reactions in which O^-₂takes part as a reactant, it can be inferred that the O^-₂-origenated oxygen species, such as hydroxyl radical, and hydrogen peroxide, may be also involved in the stress. The time-sequential analysis of antioxygenic enzymatic activities in the tow temperature (5℃ )-treated rice plants showed that the activities of superoxide dismutase and catalase, but not glutathione peroxidase, were markedly enhanced in accordance with the increase of O₂ level in leaves. In addition, experimental evidence that these enzymes in tissue are substrate-inducible were also obtained. From these observations, It is tempting to suggest that active oxygen species(O^-₂and O^-₂-originated species) are the major chemical intermediates, which cause damage to biological apparatuses in plant cells, and that SOD and catalase are the crucial constituents of defense system in plant cells against several environmental stresses.