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김재환,김영록,김해영,Kim, Jae-Hwan,Kim, Young-Rok,Kim, Hae-Yeong 한국식물생명공학회 2011 식물생명공학회지 Vol.38 No.2
1994년 처음으로 GM 토마토인 Flavr Savr가 시장에 나온 이후, 2010년 현재 140여 품목의 GM식물이 전 세계적으로 상업화되었다. GM식물들에 대한 안전성 승인여부의 확인 및 표시제관리를 위하여 이들 GM식물내로 도입된 삽입유전자의 정보를 이용한 검정방법이 도입되었으며, 또한 도입유전자의 발현된 단백질을 분석하기 위하여 정성 및 정량을 위한 면역학적 방법이 도입되었다. 본 총설에서는 국내 외적으로 개발된 콩, 옥수수, 카놀라, 면화 등의 GM식물에 적용된 multiplex PCR, real-time PCR 방법과 최신 개발 중인 microarray, 나노기술 등을 활용한 방법들을 조사하였다. Since the first commercial GM plant, the FlavrSavr tomato, authorized in 1994, more than 140 GM plants were authorized for marketing globally. For the authorization and labelling of GM plants, the detection methods for genes introduced and proteins expressed in GM plants were developed qualitatively and quantitatively. This review presented the detection methods, conventional PCR, multiplex PCR and real-time PCR, for soybean, maize, canola and cotton as the dominant GM plants. Also, microarray assay and nanotechnology as new approaches for detection methods for GM plants were investigated.
김재환,김상우,조원모,강희설,조영무,Kim, Jae-Hwan,Kim, Sang-Woo,Cho, Won-Mo,Kang, Hee-Seol,Cho, Young-Moo 한국축산환경학회 1996 축산시설환경학회지 Vol.2 No.2
The objective of this study was to estimate the investment limit in equipment capital by Capital Recovery Method. The data used was collected from 23 Hanwoo(Korean Native Cattle) farms in 1994. The necessary time for herd size expansion was average 5 years from opening(9.7 heads) to 50 heads, 4 years from 50 to 100heads, 3 years from 100 to 150 heads, and 2 years from 150 to 200 heads, respectively. It took 14 years from opening to 200 heads of herd size. The debts for fattening cattle farms were 15.2million won for the size of 50 heads, 37.1 million won for the size of 100 heads and 89.0 million won for the size 200 heads, respectively. Average rates of debt interest were 5.1 % 7.2 and 10.8, correspendingly. As the hear size increased, debt interest rates also increased due to government funds limit. The investment limits in equipment capital perfarm household were 77.7 million won for 50 heads, 135.1 for 100 heads, and 294.3 for 200 heads for 5,500 won per kg liveweight, 70.7 million won for 50 heads, 122.6 for 100 heads and 269.3 for 200 heads for 5,000 won per kg liveweight, 63.6 million won for 50 heads 110.0 for 100 heads and 244.2 for 200 heads for 4,500 won per kg liveweight.
김재환,김해영,Eun-Hee Kim,예미지 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.2
A multiplex polymerase chain reaction (PCR) detection kit for screening of four herbicide-tolerant genes (cp4 epsps,mepsps, pat, and bar) in genetically modified (GM) crops was developed. The kit was validated by three different laboratories,and the expected targets were specifically observed in 14 different herbicide-tolerant GM events. This method can be effectively and conveniently used to monitor approved and unapproved GM crops containing four herbicide-tolerant genes.
김재환,김용덕,나상준,이기욱,윤보라,Kim, Jae-Hwan,Kim, Yong-Duk,Na, Sang-Jun,Lee, Kee Ook,Yoon, Bora 대한임상신경생리학회 2015 Annals of Clinical Neurophysiology Vol.17 No.2
Brachial radiculoplexitis is characterized by acute onset of shoulder and arm pain followed by weakness and sensory loss. Brachial radiculoplexitis by herpes zoster is a rare disease, which can be diagnosed by careful history, electrodiagnosis and MRI. It has remained uncertain about clinical characteristics, treatment, and prognosis. Better understanding of this disease helps earlier diagnosis and prompt treatment to minimize neurologic sequale. We present two cases of subacute brachial radiculoplexitis preceded by herpes zoster infection.
Multiplex Real-Time PCR Kit for Qualitative Detection of Genetically Modified Roundup Ready Soybean
김재환,Jin-Hyuck Kim,Myo-Ah Baek,Hae-Yeong Kim,Yong-Suk Nam 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.1
A multiplex real-time polymerase chain reaction (PCR) kit was developed for the qualitative detection of the Roundup-Ready Soybean (RRS). This system consists of a screening assay for cauliflower mosaic virus 35S promoter and nos terminator, and an event-specific assay for RRS containing the lectin gene as an internal positive control. The limit of detection value was determined to be approximately 0.1%.
원자력 발전소 사고관리 직무의 인간신뢰도분석을 위한 수행영향인자의 선정
김재환,정원대,Kim, Jae-Hwan,Jeong, Won-Dae 대한인간공학회 2001 大韓人間工學會誌 Vol.20 No.2
This paper deals with the selection of the important Influencing Factors (IFs) under accident management situations in nuclear power plants for use in the assessment of human errors. In order to achieve this goal, we collected two types of IF taxonomies, one is the full set IF list mainly developed for human error analysis. and the other is the IFs for human reliability analysis (HRA) in probabilistic safety assessment (PSA). Five sets of IF taxonomy among the full set IF list and ten sets of IF taxonomy among HRA methodologies were collected in the study. From the review and analysis of BRA IFs, we could obtain some insights for the selection of HRA IFs. By considering the situational characteristics of the accident management domain, candidate IFs are chosen. Finally, those IFs are structured hierarchically to be appropriate for the use in the assessment of human error under accident management situation. Three nuclear accidents such as TMI. Chernobyl and JCO were analysed to validate the proposed taxonomy.
김재환,이명성,이재만,이미혜,박성미,Kim, Jae-Hwan,Lee, Myeong-Seong,Lee, Jae-Man,Lee, Mi-Hye,Park, Sung-Mi 국립문화재연구소 2010 保存科學硏究 Vol.31 No.-
This study were performed thermal shock test for four kind of different rocks (Iksan granite, Namsan granite, Jeongseon marble, Yeongyang sandstone), and according to heating temperature($400^{\circ}C$, $600^{\circ}C$) on samples were investigated physical properties such as specify gravity, porosity, p-wave velocity. As a result, the tendency was appeared that porosity increased, and specific gravity and p-wave velocity decreased at a more higher temperature. But, the situation of change appeared characteristic according to temperature and rock types. In the case of Yeongyang sandstone, it appeared in especially porosity increasing at $400^{\circ}C$. The specific gravity was little change in the all the rock at $400^{\circ}C$ but the decreased at $600^{\circ}C$. Therefore the specific gravity in the temperature range is due to the relatively small impact on the change is expected. Porosity of the granite at $400^{\circ}C$ changes little. but marble in the rate of change is large. Conversely, the sandstone porosity decreased. At $600^{\circ}C$ increased porosity in all of rocks. particularly sandstone the smallest increase in porosity. Experiments showed that p-wave velocity measured through dry rocks was sensitive to quantify the thermal damage. The p-wave velocity of all rocks decreased with increasing temperature. In the relation between porosity and p-wave velocity, p-wave velocity decreased with increasing porosity. On the other hand, in case of Yeongyang sandstone p-wave velocity decreased with decreasing porosity. thus, development of microcracks more affects p-wave velocity than porosity. In this study, damage intensity was well explained with porosity and p-wave velocity values depending on temperature increase.
ECR 플라즈마 화학기상증착법에 의한 0.12nm $\textrm{SiO}_2$ 환산두계를 갖는 Pb(Zr, Ti)$\textrm{O}_3$유전박막의제조
김재환,김용일,위당문,이원종,Kim, Jae-Hwan,Kim, Yong-Il,Wi, Dang-Mun,Lee, Won-Jong 한국재료학회 1997 한국재료학회지 Vol.7 No.8
ECR-PECVD법을 사용하여 450-49$0^{\circ}C$이하의 온도에서 Pt/SiO$_{2}$/Si기판 위에 PZT 박막을 증착하였다. 기판 온도가 46$0^{\circ}C$ 이하일 경우에는 페로브스이트 상과 제2상으로 이루어진 박막이 성장하였으며 기판온도가 47$0^{\circ}C$이상일 때에는 페로브스카이트 단일상의 PZT 박막이 성장하였다. 49$0^{\circ}C$에서 매우 얇은 페로브스카이트의 PZT 박막을 증착한 후 $650^{\circ}C$에서 1분간 raped thermal annealing(RTA) 처리한 결과 박막의 조성과 결정성에는 거의 변화가 없었으나 박막의 전하 저장 밀도는 크게 향상되었다. 이는 RTA 처리에 의한 저유전 계면층의 소멸이 주된 이유라고 판단된다. 열처리 후 두께 40-45nm의 PZT박막은 200kV/cm의 전장 하에서 $10^{-6}$$\textrm{cm}^2$이하의 누설전류값을 갖고 있었으며, 인가전압 1V에서 300fF/$\mu$$m^2$의 정전용량, 즉 SiO$_{2}$환산두께 0.12nm를 나타내었다.
김재환,박치호,곽정훈,최동윤,정광화,정의수,정연배,유용희,Kim, J.H.,Park, C.H.,Kwag, J.H.,Choi, D.Y.,Jeong, K.H.,Chung, U.S.,Chung, Y.B.,Yoo, Y.H. 한국축산환경학회 2008 축산시설환경학회지 Vol.14 No.1
본 연구는 공공부문의 효율적 투자를 위하여 장기비용함수에 의한 가축분뇨공공처리시설의 적정규모를 산출하고자 수행하였다. 분석에는 2007년도 환경부에서 수집된 52개소의 자료를 이용하였다. 현재의 주어진 여건하에서의 경제적 적정규모는 분석대상 시설평균 $146m^3$/일의 $1.5{\sim}1.6$배에 달하는 $180{\sim}200m^3$/일 인 것으로 산출되었다. 이는 향후 규모 확대의 가능성을 시사하고 있어 공공부문의 투자효율 극대화와 자연순환농업의 조기 달성을 위해서는 가축사육 과밀(양분초과)지역을 우선 대상으로 하되, 지역 내 자원화시설 등 관련 시설과의 연계성과 경제적 적정규모를 고려하여 시설투자 계획을 수립해야 할 것으로 사료된다. The objective of this study was to estimate the optimum scale of PLMTP (Public Livestock Manure Treatment Plant) for the efficient management of public sector by long-run cost function. An economic analysis was performed using the survey of 52 PLMTP records collected by Ministry of Environment in 2007. The main results obtained in this study can be summed up as follows. The optimum scale under given environmental conditions turned out to be $180{\sim}200m^3$/day which is almost $1.5{\sim}1.6$ times of the average scale of sample plants, $146m^3$/day. This gap between the optimum and current scale suggests that there remains the possibility of further expansion of scale.