인체 진피 미세혈관 내피세포 표면에서 v - cadherin의 발현에 관한 연구

김이선,최현주,이민걸,이광훈 ( Yi Sun Kim,Hyun Joo Choi,Min Geol Lee,Kwang Hoon Lee ) 대한피부과학회 1997 大韓皮膚科學會誌 Vol.35 No.5

Background: Cadherins are a supergene family of structurally related transmembrane glycoprotein. V-cadherin was a subclass of cadherin which expressed on endothelial cell as an endothelial cell cell adhesion molecule. The role of V-cadherin is still unclear in inflarnmation and immunologic responses. Objective : Our purpose was to demonstrate the expression and the regulation of V-cadherin expression on human dermal microvascular endothelial cells(HDMEC) by biological response modifier(BRM). Methods : The cell surface expression of V-cadherin on HDMEC that was unstimulated or pretreated with IL-1, TNF, IFN-, and PMA was evaluated by immunofluorescence flow cytometry and the enzyme-linked immunosorbent assay. Results : 1. V-cadherin was constitutively expressed on unstimulated HDMEC. 2. Maximal expression of V-cadherin was observed at 16 hrs after stimulation with IL-1, at 6 hrs after stimulation with TNF and at 24 hrs after stimulation with IFN- on HDMEC. 3. V-cadherin expression on HDMEC was increased after stimulation with IL-1 or PMA. Conclusion : V-cadherin was constitutively expressed on HDMEC, and V-cadherin expression can be regulated by BRM. These results suggest V-cadherin may contribute to inflammation. (Korean J Dermatol 1997;35(5): 924-930)

공간에 점령당하지 않는 힘

김이선,Kim, Lee-Seon 대한출판문화협회 2003 출판저널 Vol.327 No.-

삼색성 백반증의 임상 및 병리조직학적 특성

김이선,한승경 ( Yi Sun Kim,Seung Kyung Hann ) 대한피부과학회 1997 大韓皮膚科學會誌 Vol.35 No.4

Background: The term trichrome vitiligo was first suggested in 1964 by Fitzpatrick, There is a tan zone of varying width between the normal and the totally depigmented skin and this exhibits the intermediate hue. The etiopathogenesis and the histopathological characteristics of trichrome vitiligo are unknown. Objective : Our purpose was to evaluate the clinical and histopathological characteristics of trichrome vitiligo. Methods . Four punch biopsies were taken from twenty-one patients with trichrome vitiligo; from vitiliginous skin, light brown skin, perilesional normal skin and normal skin as far as 5cm from the nearest vitiligo spot. The sections were stained with hematoxylin-eosin and S-100 protein. Results : 1. Trichrome vitiligo occurred mostly on the trunk in active vitiligo vulgaris. 2. Vacuolar degeneration of the basal cell layer in the epidermis and mild inflammatory cell infiltration in the epidermis and dermis were more prominent in light, brown skin and perilesional normal skin than in vitiliginous skin and normal skin of trichrome vitiligo. 3. The number of melanocytes was decreased in light brown skin compared with perilesional normal skin(p<0.05), and in vitiligonous skin compared with light brown skin(p<0.05), 4. A few melanocytes were also observed in vitiliginous skin of trichrome vitiligo. 5. In the trichrome vitiligo, the result of treatment was excellent in cases of systemic PUVA therapy. Conclusion : Melanocytes, keratinocytes and lymphocytes may be involved in the genesis of depigmentation in trichrome vitiligo, which means cell-mediated immunity may play an important role in the pathogenesis of trichrome vitiligo. (Kor J Dermatol 1997;35(4): 720-728)

참외 껍질 유무에 따른 Acrinathrin, Diflubenzuron, Mandipropamid, Pyraclostrobin 잔류량 비교

김이선(Leesun Kim),박정훤(Jeong-Hwon Park),노현호(Hyun-Ho Noh),김창조(Chang-Jo Kim),김단비(Dan-Bi Kim),권혜영(Hyeyoung Kwon),진용덕(Yong-Duk Jin),노진호(Jin-Ho Ro) 한국농약과학회 2021 농약과학회지 Vol.25 No.3

LC-MS/MS를 이용한 갈색거저리 유충 중 Fenoxanil, Thiobencarb 및 Fludioxonil의 잔류분석

김이선 ( Leesun Kim ),백수진 ( Sujin Baek ),손경애 ( Kyungae Son ),노현호 ( Hyunho Noh ),문병철 ( Byeong-chul Moon ),김단비 ( Danbi Kim ),오민석 ( Min-seok Oh ),노진호 ( Jin-ho Ro ),김은선 ( Eunsun Kim ) 한국환경농학회 2020 한국환경농학회 학술대회집 Vol.2020 No.-

This study was designed to determine fenoxanil, thiobencarb, and fludioxonil residues in mealworms, the larvae of Tenebrio moliter using liquid chromatography tandem mass spectrometry. These pesticides are officially applied for wheat cultivation, so can be found in wheat bran, recommended feed for mealworms in South Korea. In this regard, effective analytical method is urgently required because mealworms are used as food or feed as alternative protein sources. Acetonitrile (10 mL) was used to extract the target pesticides. Four clean-up procedures ((1) MgSO₄+25 mg PSA+25 mg C18 (2) MgSO₄+50 mg PSA+50 mg C18 (3) EMR-lipid™ tube, and (4) 10 mL n-hexane) were examined. The procedure (1) successfully removed lipids and proteins derived from insects with the satisfied recoveries of the target analytes at two spiking levels of 5 ㎍/kg and 10 ㎍/kg. The recoveries were 87.6-112.9 ± RSD 4.2-4.6% for fenoxanil, 83.3-87.6 ± RSD 1.3-4.6% for thiobencarb, and 76.9~82.0 ± RSD 3.4-7.2% for fludioxonil. Method detection limit was 1.0 Mg/L. The optimized method was also applied to real samples from 30 different farms across the nation. These data can be used to set maximum residual limit for the residues in edible insects in response to positive list system. The method developed in this study is expected to be also applied to other edible insects or matrices having high fat and protein contents.

멀티콥터로 살포한 dimethomorph의 쪽파 중 잔류 및 분포 특성

김창조(Chang Jo Kim),이래근(RaeKeun Lee),신희정(Hee Jeong Shin),문병철(Byeong-chul Moon),김흥태(Heung Tae Kim),김이선(LeeSun Kim),김단비(Danbi Kim),이희동(Hee-Dong Lee),경기성(Kee Sung Kyung),노현호(Hyun Ho Noh) 한국농약과학회 2021 농약과학회지 Vol.25 No.2

고령친화형 식품의 제도적 시스템 마련에 대한 식품산업체 인식 조사 연구

양윤경 ( Yoon Kyoung Yang ),김이선 ( Leeseon Kim ),김소운 ( Sowoon Kim ),김정선 ( Jeong Seon Kim ),김범근 ( Bum-keun Kim ),김지연 ( Ji Yeon Kim ) 한국산업식품공학회 2018 산업 식품공학 Vol.22 No.2

고령친화형식품에 대한 법적 제도마련을 위한 기준규격개발의 기반자료를 마련하기 위한 산업체 대상 설문조사의 결과는 다음과 같다. 노인을 타깃으로 생산하거나 판매하는 식품이 있느냐의 결과는 ‘아니요’ 48명(50.4%)으로 가장 많이 나타났다. 노인 타깃 식품 유형은 건강기능식품이 19명(40.4%)이고 노인 타깃 식품 생산·판매 희망여부는 52명(54.7%)이었다. 고령자를 위한 식품으로 필요한 식품은 고른 영양소를 섭취할 수 있는 식품, 기능성 원료를 강화한 식품, 저작의 편의를 도모한 식품 순으로 선택하였다. 노인 식품에 대한 법적 지위 필요여부는 47명(51.1%)이 필요하다고 하였으며, 고령친화제품 인증이 회사의 판매에 가장 유리할 것으로 판단한다고 답을 하였다. 고령자를 위한 식품의 인정에 필요한 기준은 저작의 편의를 도모한 물성기준, 건강을 고려한 기능성 원료 첨가에 대한 기준, 영양소 기준, 위해 및 안전 기준 모두가 필요하다고 답한 경우가 가장 많았다. 저작 능력에 따라 5가지 단계로 구분하는 일본 개호식품을 참고로 우리나라에서도 고령자를 위한 식품의 기준규격을 설정하는데 있어 5단계로 구분하는 것이 타당하다고 생각하느냐에 대해서는 41명(44.6%)명이 답을 하였다. 고령자 식품 활성화 방안은 인증제도 운영이 가장 높은 점수를 나타냈다. 본 연구결과를 토대로 향후 국내에서도 고령자를 위한 식품 유형의 신설이나 세금 우대 혜택 등의 활성화 방안이 만들어져야 할 것으로 판단된다. The objective of this study was to develop a standard for setting a legal system for senior-friendly food. We conducted a web-based survey on 95 food industry workers. Answers to 10 questions in the survey were analyzed using SPSS 19.0. Important factors to consider when it comes to food for seniors are the supply of balanced nutrition, enhancement of functionality, and promotion of convenience. A total of 47 respondents (51.1%) felt the need for a legal system for senior food, and they thought that a certification for senior-friendly food would be advantageous for companies. They said that there should be texture standards to promote ease of chewing, standards for functionality, nutritional standards, and safety standards to certify senior-friendly foods. A total of 41 people (44.6%) said that it was reasonable to classify Japanese food into five levels of senior-friendly foods according to ease of chewing. Certification management received the highest score in implementing an activation plan for senior-friendly food.

상품과 비상품 사이에서: 관광 발달과 반타와이 목공예의 의미체계 변화

김이선 ( Yi Seon Kim ) 한국문화인류학회 2004 韓國文化人類學 Vol.37 No.1

토종 복분자 Rubus coreanus와 외래종 복분자 Rubus occidentalis 추출물의 항산화능 비교

김이선(Lee Seon Kim),윤상혁(Sang Hyuck Youn),김지연(Ji Yeon Kim) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.9

본 연구에서는 토종 복분자와 외래종 복분자의 항산화 활성과 장세포 산화스트레스 보호의 차이를 알아보기 위해 총 폴리페놀 및 플라보노이드 함량, DPPH 및 ABTS 라디칼 소거능, FRAP assay를 이용한 환원력을 비교 조사하였고, 사람의 장내 세포인 Caco-2 cell에 과산화수소를 이용하여 자극시켜 장세포 산화스트레스 보호를 확인하였다. 총 폴리페놀 및 플라보노이드 함량은 외래종 복분자가 유의적으로 높은 것으로 나타났다. ABTS 라디칼 소거능과 FRAP assay을 이용한 환원력에서도 외래종 복분자가 유의적으로 높은 것으로 나타났으나 DPPH 라디칼 소거능은 토종과 외래종 복분자 모두 비슷하게 나타났다. 장세포 산화스트레스보호 역시 외래종 복분자가 유의적으로 높게 나타났다. 항산화 성분과 항산화 활성의 상관관계는 모두 유의하게 양의 상관관계를 보이는 것으로 나타났으며, FRAP value가 가장 높은 상관관계를 보이는 것으로 확인되었다. 따라서 FRAP value가 높았던 외래종 복분자 100% EtOH 추출물이 폴리페놀 함량, 플라보노이드 함량, ABTS 라디칼 소거능, DPPH 라디칼 소거능, 장세포 산화스트레스 보호능 모두 높은 추출물로 확인되었다. This study compared the antioxidant effects of two kinds of black raspberry extract, obtained from fruits of Rubus coreanus and Rubus occidentalis, which can be found in Korea. The fruits of R. coreanus and R. occidentalis were each extracted with 0%, 25%, 50%, 75%, and 100% ethanol (EtOH). Among the extracts of these two varieties, 50% EtOH extract of R. occidentalis showed the highest contents of total polyphenols (46.96±2.78 mg/g) and flavonoid compounds (11.77±0.81 mg/g). The 50% EtOH extract of R. occidentalis showed the highest antioxidant activity (84.77±0.97%) in terms of DPPH radical scavenging activity. On the contrary, 25% EtOH extract of R. occidentalis showed the best antioxidant activity (29.65±2.41%) in terms of ABTS radical scavenging activity. In the results of ferric reducing antioxidant power (FRAP) assay, 50% EtOH extract of R. occidentalis showed the highest antioxidant activity (0.49±0.02%). In the cytotoxicity test stimulated with H₂O₂, the extracts of 75% and 100% EtOH from R. occidentalis showed the highest cell viability (93.54±3.37% and 97.19±0.74%, respectively). According to our results, extracts of R. occidentalis showed higher antioxidant activities than extracts of R. coreanus. Especially, total polyphenol and flavonoid contents of R. occidentalis showed the highest significant correlation with FRAP by Pearson"s correlation (P=0.005 and P=0.013, respectively).

Deoxycholic Acid 유도 장세포 염증성 손상에 대한 어린감과 성숙감 추출물들의 보호 효과 비교

김이선(Leeseon Kim),권오란(Oran Kwon),김지연(Ji Yeon Kim) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.10

본 연구에서는 어린감과 성숙감의 탄닌 함량 및 담즙산과의 결합능력을 비교 조사하였다. 또한 사람의 장내 세포인 Caco-2 cell에 어린감과 성숙감 추출물을 처리한 후 고농도의 DCA로 자극시켜 생성되는 NO의 수준과 TEER의 차이를 확인하였다. 탄닌 함량과 담즙산의 결합능력은 성숙감에 비해 어린감에서 유의적으로 높은 것으로 나타났으며 어린 감 산성 에탄올 추출물에서 유의적으로 가장 높은 탄닌 함량 및 담즙산 결합능력을 보였다. NO 생성 억제능으로 확인한 염증반응에서도 어린감 산성 에탄올 추출물이 유의적으로 가장 높은 효과를 보였으며 DCA에 의해 유도된 장세포 간극의 느슨함을 유의하게 막아줄 수 있음이 확인되었다. 본 연구 결과를 토대로 어린감의 산성 에탄올 추출물은 고지방 식사를 통해 고농도로 축적되는 담즙산에 의해 형성된 유해한 장내 환경을 건강하게 유지시켜줄 수 있는 기능성 소재로 개발할 가능성이 있음이 확인되었다. Bile acids are endogenous metabolites that aid in the digestion and absorption of ingested fat and fat-soluble vitamins. However, high concentrations of deoxycholic acid (DCA) in the colon are associated with high incidence of colorectal cancer. In the present study, the binding of persimmon extracts to DCA in order to decrease inflammatory stress induced by DCA in a small intestinal epithelial cell line, Caco-2, was investigated. Young and ripened persimmons were extracted with distilled water (DW), ethanol, and acidic ethanol. Further, DW extract residue was re-extracted with acidic ethanol. Of the obtained extracts, acidic ethanol extract of young persimmon showed the highest bile-acid binding capacity. Moreover, acidic ethanol extract of young persimmon significantly inhibited nitric oxide production in Caco-2 cells stimulated with DCA and prevented significant reduction of trans-epithelial electric resistance. Based on these results, acidic ethanol extract of young persimmon can be used as a functional ingredient to enhance gastrointestinal health.