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김원근,이찬원,전홍표 경남대학교 환경문제연구소 2002 환경연구 Vol.25 No.-
A field study was conducted to investigate the removal efficiency and performance of contact oxidation treatment system installed and operated in two locations of stream flowing to the Masan Bay. The removal of organic matters from stream water flowing to the already eutrophicated bay is getting more important in order to restore the water quality. The removal efficiencies of BOD, SS and ABS loading rate were 52~63%, 50~70% and 60~85%, respectively, whereas 10~25% of total nitrogen and 25~40% of total phosphorus were only removed. The effluent water quality did not meet the goal class of water regulation standards. Inflow rates to contact oxidation treatment showed seasonal and hourly variations. The treatment capacity installed is now enough to handle the present inflows, thus it was suggested that this system should be operated to supply only the volume of oxygen to require the actual loading to the treatment system and the aeration system would be intermittently operated for the improvement of nitrogen and phosphorus removal. It was found that inflow rate should be controlled for detention time of contact oxidation to maintain at least more than 1.5 hr. When microbiological species of sludge and sludge accumulation in the lower steps of contact oxidation process was examined, proper draw-out of sludge from the system was also an important operating parameter.
재조합 대장균과 효모의 고정화 혼합세포계에 의한 ${\gamma}$-Glutamylcysteine 생산
김원근,구윤모 한국생물공학회 1995 KSBB Journal Vol.10 No.3
${\gamma}$-Glutamylcysteine 생산에 있어서 재조합 대장 균 HB101/pGH501만을 이용한 단일세포반응계가 재조합 대장균과 효모를 이용한 흔합서l포반응계보다 반응시간이 짧고 생산농도가 높은 것으로 나타났다. 그러나 생산경제성 측면에서 ATP 재생공정을 위하 여 훈합세포반응계를 사용하였다. 재조합 대장균과 효모를 이용한 혼합세포반응계에서 대장균과 효모의 비율은 1:4가 적합함을 보였고, ATP 재생공정에 사용되는 glucose는 O.5M의 농도에서 가장 효율적 으로 나타났다. 재조합 대장균과 효모를 alginate를 이용하여 고정화하여 반응계로 사용하였을 경우 반 응에 필요한 시간이 걸어지고 생산놓도도 감소되냐 반응계의 안정성은 10% 정도 증가됨을 알 수 있었다. 실험결과 alginate로 고정화된 흔합세포반응계 를 사용하여 ${\gamma}$-glutamylcysteine를 연속 생산할 수 있음을 확인하였다. ${\gamma}$-Glutamylcysteine production by the immobilized microbial system of recombinant Escherichia coli and yeast was investigated. ${\gamma}$-Glutamylcysteine was synthesized from L-glutamic acid and L-cysteine in the presence of ATP by the reaction catalyzed by ${\gamma}$-glutamylcysteine synthetase. An immobilized microbial cell system was developed for the efficient ${\gamma}$-glutamylcysteine production. Recombinant Escherichia coli and yeast were immobilized by alginate. Production of ${\gamma}$-glutamylcysteine was better with the recombinant Escherichia coli for both the synthesis of ${\gamma}$-glutamylcysteine and the ATP regeneration than the mixed system of recombinant Escherichia coli and yeast. The proper radio of recombinant Escherichia coli to yeast was experimentary observed to be 1:4 in the mixed system. Although the immobi1ized system had the slower reaction rate, its reaction stability was increased by 10%.
김원근,Kim, Wan-Kuen 한국생명보험의학회 1986 保險醫學會誌 Vol.3 No.1
Liver is an oragan which has various functions. For this functions in liver disease change variously, many methods of examination of them are invented. Each liver disease impairs these functions diversly rather than uniformly, thus it is possible to differentiate each disease by using various methods of testing it. Liver has high reproductive capacity and despite partial impairment of its function localized disease may not show any abnormality in liver function test due to compensation of remaining intact tissue of the liver. Thus the result of liver function test should be interpreted under adequate clinical investigation with other test, not depending on the liver function test only. This thesis deals with the composition, distribution in living body and clinical significance of liberating enzyme, GOT, GPT, and LDH which are included in serum enzyme that show abnormality in liver disease.