http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Molecular and Cellular Studies of Seed Storage Proteins from Rice and Wheat
김우택 한국농화학회 1989 Applied Biological Chemistry (Appl Biol Chem) Vol.32 No.1
Near full length cDNA clones encoding the rice seed storage protein, prolamine, were isolated and divided into two homology classes based on cross-hybridization and DNA sequencing analysis. These cDNA clones contain a single open reading frame encoding a putative rice prolamine precursor(M.W.=17,200) possessing atypical 14 amino acid signal peptide. Clones of these two homology classes diverge mainly by insertions/deletions of short nucleotide stretches and point mutations. The deduced primary structures of both types of prolamine polypeptides are devoid of any major tandem repetitive sequences, a feature prevalent in other cereal prolamines. No significant homology teas detected between the rice prolamine and other cereal prolamines, indicating that the rice gene evolved from a different ancestor that gave rise to other cereal prolamine genes. Developing wheat and: rice endosperms were examined using ultrathin sections prepared from tissues harvested at various days after flowering. By immunocytochemical localization techniques, wheat prolamines are localized within vesicles from Golgi apparatus and in homogeneous regions of protein bodies. The involvement of the goli apparatus in the packaging of wheat prolamines into protein bodies indicates a pathway which differs from the mode of other cereal prolamines and resembles the mechanism employed for the storage of rice glutelin and legume globulins.
김우택,최현민,류민형,이지형,조이상,조진수 한국항공우주학회 2015 한국항공우주학회 학술발표회 논문집 Vol.2015 No.4
본 연구는 블레이드 주위를 링으로 감싸 회전하는 “팁마운티드 블레이드”의 유동특성에 따른 구조적 안정성을 평가하기 위해 단방향 유체-구조 연성해석(Fluid-Structure Interaction, FSI)을 수행하였다. 덕티드팬의 순항 및 이륙시의 공력하중을 구조해석에 적용하였다. 덕티드팬 블레이드는 폴리카보네이트로 제작되는 것을 가정하여 구조해석을 수행하였다. 최대응력 및 최대변위를 분석하여 구조적 안정성을 평가하였다. 일반적인 프로펠러와는 달리 최대변위가 스팬의 중간지점에서 발생하였으며, 등가최대응력은 익근과 익단부위에서 발생하는 것을 확인하였다. 폴리카보네이트의 물성을 적용했을 때 안전율이 안정적인 것으로 평가되었다. FSI analysis is performed to evaluate a structural stability of the tip mounted blade, that is wrapped by the ring, for the ducted fan. The aerodynamic load of ducted fan is adopted to structural analysis at cruise and take off condition. The material property of the polycarbonate is applied for the ducted fan. Structural stability is evaluated using the equivalent maximum stress and the maximum displacement. Unlike typical propeller, the maximum displacement occurs at the midpoint of the span. The equivalent maximum stress was confirmed that generated on the tip and root. Safety factor when applying the physical properties of polycarbonate were evaluated to have been stabilized.
오리의 Uropygial Gland 에서 분리한 Glucose - 6 - phosphate Dehydrogenase Isozyme 의 Kinetic 특성
김우택,서경훈,김유삼 ( Woo Taek Kim,Yyong Hoon Seo,Yu Sam Kim ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.4
One isozyme of glucose-6-phosphate dehydrogenase with pI of 6.6 was purified from the uropygial gland of Peking duck and its kinetic properties were examined. The reaction by the enzyme followed ordered bi-bi reaction mechanism which was also detected in the reaction by the purified liver G6-PD of the same duck. Km values to G-6-P and NADP+ for the gland G6-PD were 101 μM and 4. 41 μM, respectively. And for the liver enzyme, Km to G-6-P was 76.92 μM and to NADP+ was 9.09 μM. Km to substrate analogs, galactose-6-phosphate and 2-deoxy-D-glucose-6-phosphate, for the gland enzyme were 6.7×10³ ACM and 3.65×10³ μM, respectively. NAD+ was not used as a coenzyme by the gland even though its concentration was raised up to 9. 7 mM. NADPH, a product of the enzyme reaction, was competitive inhibitor to NADP+ with Ki of 42.5 μM for the gland enzyme. On the other hand, that Ki of the liver enzyme was 25 μM. For the gland enzyme, glucosamine-6-phosphate was an uncompetitive inhibitor to NADP+ and ATP was a noncompetitive one. These results, the high substrate affinity and high Ki value of NADPH for the gland enzyme, indirectly indicated that the gland G-6-PD was well designed enzyme for the active and continuous production of surface-lipids in the uropygial gland.