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      • KCI등재

        Changes of the Ultrastructure and $Ca^{2+}$ Distribution after Transient Ischemia and after Reperfusion in the Myocardial Cells of Isolated Perfused Guinea Pig Hearts

        김용문,김호덕,라봉진,Kim, Yong-Mun,Kim, Ho-Duk,Rah, Bong-Jin Korean Society of Electron Microscopy 1989 Applied microscopy Vol.19 No.1

        허혈로 인하여 손상을 받은 심근세포의 기능을 재건하기 위하여 재관류가 반드시 이로운가하는 것에 대하여는 논란이 많다. 따라서 저자는 체중 500그람 내외의 기니피그를 실험재료로 하여 일과성허혈(10분) 및 일과성허혈후 재관류시(20분) 좌심실 심근세포의 미세구조 및 칼슘분포의 변화를 비교관찰하여 재관류가 심근세포재건에 어느정도 도움을 줄 수 있는가를 알아보고자 하였다. 대조군에서는 심근세포의 미세구조가 비교적 잘 보존되어 있었으며 심근세포막 및 사립체내에서 20nm 크기 정도의 칼슘침착을 볼 수 있었다. 이에 반하여 일과성허혈후, 대부분의 심근세포에서는 세포막손상, I 대의 확장, 사립체의 종창, 세포내 수분축적, 당원과립의 고갈, 지방소적유령(ghost)의 출현, 염색질의 응집 및 변연부로의 이동, 세포연접의 분리 등의 미세구조의 변화와 심근세포막 및 사립체내에서 칼슘침착의 현저한 감소를 볼 수 있었으나, 비가역성의 변화는 찾아 볼 수 없었다. 그러나 허혈후 재관류시 일부의 심근세포에서는 큰 변화를 보이지 않았지만, 많은 세포에서 이러한 미세구조의 변화는 보다 심해져 국소적으로 근절(sarcomere)의 과이완 및 striation pattern의 소실, 세포부종 등이 현저해졌으나, 세포막 및 사립체내에서는 대조군에서와 같이 칼슘침착이 재출현하였다. 이상의 결과로 미루어 일과성허혈후 재관류는 심근세포의 칼슘 조절기능 회복에 어느정도 도움을 줄 수 있으나 허혈성 손상을 악화시킬 가능성도 있을 것으로 생각된다. It has been debated whether postischemic reperfusion is necessarily beneficial to salvage the myocardium after ischemic insult or not. Therefore, this study was undertaken to compare the ultrastructural changes as well as the distribution of $Ca^{2+}$ in the ventricular myocardial cells after transient ischemia and after postischemic reperfusion, and to suspect to what extent the postischemic reperfusion is beneficial. After 10 minutes of ischemia, the heart developed wide I bands, glycogen depletion, intramyofibrillar edema, mitochondrial swelling, clumping and migration of chromatin, ghosts of lipid droplets, disintegration of cell junctions, sarcolemmal disruption, and loss of $Ca^{2+}$ binding capacity of the sarcolemma and the mitochondria. In spite of reperfusion, in a large number of cells, the ultrastructure was more severely damaged, however, $Ca^{2+}$ binding capacity of the sarcolemma and the mitochondria restored. These results suggest that postischemic reperfusion may help the myocardial cells to restore their function to control $Ca^{2+}$ to a certain extent, but that it could aggravate the ischemic insult.

      • KCI등재

        흉노(匈奴)의 복식문화에 관한 연구

        김용문(Yong Mun Kim) 한국복식학회 2013 服飾 Vol.63 No.3

        Xiognu people were the first of the Central-Asian nomads to establish a nation in 209 B.C. They always moved around looking for places to breed their animals and fertile grounds, so they wore clothes made of fur and leather and covered their tents with felt from the livestock. This research studies on the literatures, costumes and the achievement of archaeological excavation. Furthermore, to investigate on costumes excavated of Xiongnu, we visited the Mongolian National Museum and the Hermitage Museum. A corn-hat made of felt, a felt hat with ear flaps and a golden crown with a bird on the top were unearthed from a tomb of Xiongnu in Inner mongolia. Women usually wore pigtails, and men wore pigtails or ponytails but they cut their hair short when holding a funeral. Many pigtails discovered in Noyon uul tombs can be considered as their funeral customs. The Xiongnu wore a round or v-neck caftan attached straight sleeves reaching knees in the left folded style, and because they always rode horses, having the length of the caftan not go past their buttocks would have made it more convenient for them. During the period of Western Han, Ho refered to Xiongnu and it became a common name for northern races. They used leather belts and an animal-designed buckle was found. Women commonly rouged their cheeks for a vivid and cute look, and many ornaments were excavated including bracelets, rings and decorations made of gold, silver, copper and jade, among which there were hair ornaments used to identify one`s class. A horse pattern with wings and a horn of Golmod T20 was substitution for the Schythian use of deer. Patterns or shape of unearthed articles present in the Xiongnu culture in Noyon uul had a close relationship with Altaic, Greek and Persian cultures. The Xiongnu clothing was made of animals` skin and fur, woolen textiles and felt. It was folded to the left for upper garments, and the pants were adjusted using a belt and shoes were made of leather, which was very suitable for protection against the cold and horse riding. Mobility played a significant role in their clothing.

      • KCI등재

        다단계 연소 방식을 이용한 PCP(Pentachlorophenol) 및 유기용제 제거를 위한 열처리 방법에 대한 연구

        김용문 ( Kim Yong-mun ),양고수 ( Yang Go-su ) 한국환경기술학회 2000 한국환경기술학회지 Vol.1 No.2

        A multistage burning burner was developed and designed to improve 3 T`s conditions, turbulent mixing, high and homogeneous temperature, and residence time to incinerate spent chlorine contaminated solvents, which were known as dangerous and difficult combustible compounds. The burner consisted of 3 main parts, evaporating unit, first burning zone, second burning zone. Liquid fuel was able to be fast vaporized in the vaporizing unit, and the vaporized fuel and air rapidly mixed and incinerated in the first and after second burning zone in the burner. The burner was tested and examined in terms of the efficiency of combustion and the Amount of emitted hazardous intermediate species from the flames by burning the Chlorinated compound. PCP(Pentachlorophenol), which is solid at environmental condition, Was selected as a chlorinated compound and dissolved in the diesel fuel. The concentration Range of PCP in the diesel fuel was 1.0%-1.6% as of diesel fuel volume. Gas and soot Samples collected at the downstream of the burner were analyzed with a gas Chromatography. Soot sample was extracted with C<sub>2</sub>H<sub>2</sub>Cl<sub>2</sub> before injucted into the GC. As of important results, the amount of soot was almost negligible or non detectable over All experimental conditions, but some of hazardous intermediate compounds were detected. The concentrations of the intermediates were very low at 1.0% of PCP, however they Increased as PCP was loaded more in the flame. One of important intermediates was chlorobenzene.

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