홍차추출물 함유 누드팩의 Hairless 마우스 피부주름 형성 억제효과

김영철,박은예,김상남,유용기,박미순,이귀영,이석준,장병수,Kim, Young-Chul,Park, Eun-Ye,Kim, Sang-Nam,Yoo, Yong-Gi,Park, Mi-Soon,Lee, Gui-Yeong,Lee, Suk-Jun,Chang, Byung-Soo 한국현미경학회 2011 Applied microscopy Vol.41 No.2

The aim of this study was to evaluate the inhibitory effect of nude pack containing black tea water extract (NPBT) on skin wrinkle formation in hairless mice. Skin wrinkles were induced by UVB irradiation to the backs of hairless mice for 5 weeks. And at the same time, NPBT was applied topically. Wrinkle formation, histological changes, expression of matrix metalloproteinase-3 (MMP-3) and protein activities of MMP-2 and MMP-9 were observed or analyzed. Wrinkles for the control group were formed as a pattern of deep furrows and thick crests. Whereas wrinkles for the NPBT treated group were formed as a pattern of shallow furrows and thin crests, and their wrinkle areas were significantly (p<0.001) lower than the control group. Collagen fibers were arranged irregularly and sparse in density and some elastic fibers were degenerated in the control group, while they were almost intact in the NPBT treated group. MMP-3 mRNA expression in the control group was significantly (p<0.001) higher than the normal group, and that of NPBT treated group was significantly (p<0.001) lower than the control group. The NPBT treated group showed remarkably lower protein activities of MMP-2 and MMP-9 than the control group. NPBT could have a considerable inhibitory effect on skin wrinkle formation in hairless mice. 홍차함유 누드팩의 피부주름 형성 억제효과를 알아보기 위해 SKH-1 hairless 마우스 등 부위에 UVB를 5주간 조사하여 주름을 유발시키고 이와 병행하여 홍차함유 누드팩을 도포한 실험에서 다음과 같은 결과를 얻었다. (1) 육안적인 피부주름 양상 비교에서, 대조군은 정상군에 비해 주름 능선의 두께가 굵고 간격이 넓으며 주름이 깊게 형성된 반면, retinoic acid를 도포한 양성대조군과 홍차함유 누드팩 도포군은 대조군에 비해 상대적으로 주름 능선의 두께가 얇고 간격이 좁은 양상을 띠었으며 주름 총넓이 수치가 유의하게(p<0.001) 낮았다. (2) 피부의 조직학적 관찰에서, 대조군은 정상군에 비해 진피층내 교원섬유의 배열이 불규칙하고 양이 많이 줄어들었으며 변성된 탄력섬유가 군데군데 엉겨 있는 탄력섬유증이 관찰된 반면, 양성대조군과 홍차함유 누드팩 도포군은 대조군에 비해 상대적으로 교원섬유의 배열이 규칙적이고 밀도가 조밀하며 변성된 탄력섬유의 양이 줄어 있었다. (3) 피부조직의 MMP-3 mRNA 발현량 측정 결과, 정상군에 비해 대조군은 유의하게(p<0.001) 높았으며 양성대조군과 홍차함유 누드팩 도포군은 대조군에 비해 유의하게(p<0.001) 낮았다. MMP-2와 MMP-9 단백질 활성 측정 결과, 대조군은 정상군에 비해 현저하게 높은 반면, 양성대조군과 홍차함유 누드팩 도포군은 대조군보다 현저하게 낮게 나타났다. 본 연구를 통하여, 홍차함유 누드팩 도포군은 hairless 마우스에서 세포외 기질단백질의 분해 억제와 피부주름 형성 억제효과를 보였음을 확인하였다.

스프레이 장미 ‘Sunny Lady’ 및 ‘Lovely Pinky’ 품종의 농가 시범재배와 생육, 품질 및 수량특성

김영철(Young Chul Kim),윤석규(Seok Kyu Yun),권기범(Ki Bum Kweon),박영섭(Young Seob Park),정화영(Hwa Young Jeong),김성태(Seong Tae Kim),김원희(Won Hee Kim),김재영(Jae Yeong Kim),유봉식(Bong Sik Yoo) 한국원예학회 2007 한국원예학회 학술발표요지 Vol.2007 No.6

멀티센서 퓨전 기법을 활용한 방위 측정 알고리즘의 설계

김태영(Kim, Tae-Yeong),김영철(Kim, Young-Chul),송문규(Song, Moon-Kyou),정길도(Chong, Kil-To) 한국산학기술학회 2011 한국산학기술학회논문지 Vol.12 No.2

현재 선박의 자동운항을 위해서는 항체의 위치정보 및 방위정보가 반드시 필요하다. 그 중에서도 방위정보 는 자동조타 시스템에 필수적인 요소로 측정 장비로는 자이로컴퍼스, 마그네틱 컴퍼스 그리고 GPS 컴퍼스가 있다. 자이로컴퍼스는 주로 GMDSS(Global Maritime Distress & Safety System)의 대형선박에 사용되고 있으며 정밀도와 신 뢰도가 우수하지만 부피가 크고 고가라는 단점이 있다. 그리고 마그네틱 컴퍼스는 비교적 정확하고 저렴하지만 선박 의 철제 구조물 등과 같은 자성물체에 의한 영향을 많이 받는다는 단점을 가지고 있다. GPS컴퍼스의 경우는 위치정 보의 변화에 따라 진북을 가리키지만 항체가 정지해있거나 오차범위 내에서 운동을 할 경우 정확한 방향을 얻을 수 없다. 본 논문에서는 이러한 방위각 센서의 상호보완을 위해서 least-square curve fitting 방법을 이용하여 GPS 컴퍼스의 성능을 향상시키고 이를 마그네틱 컴퍼스와 공분산 오차의 크기에 따른 가중치를 적용하여 방위각의 정밀도를 향상 시키는 알고리즘을 제안하였다. 실험을 통하여 제안된 알고리즘의 특성을 분석하고 성능을 검증하였으며 실험 결과를 통해 제안된 알고리즘의 적용 가능성을 보여주었다. Presently, the location and direction information are certainly needed for the autonomous vehicle of the ship. Among them, the direction information is a essential elements to automatic steering system. And the gyro-compass, the magnetic-compass and the GPS compass are the sensor indicating the direction. The gyro-compasses are mainly used in the large-sized ship of the GMDSS(Global Maritime Distress & Safety System). The precision and the reliability of the gyro-compasses are excellent but big volume and high price are disadvantage. The magnetic-compass has relatively fine precision and inexpensive price. However, the disadvantage is in the influence by the magnetism object including the steel structure of a ship, and etc. In the case of the GPS compass, the true north is indicated according to the change of the location information but in case of the minimum number of satellites or stopping of a ship or exercise in the error range, the exact direction cannot be obtained. In this paper, the performance of the GPS compass was improved by using the least-square curve fitting method for the mutual trade off of the angle sensor. The algorithm which improves the precision of an azimuth by applying the weighted value according to the size of covariance error was proposed with GPS-compass and magnetic compass. The characteristic and the performance of the proposed algorithm were analyzed and verified through experimentation. The applicability of the proposed algorithm was shown through the experimental result.

선박의 자동조타를 위한 향상된 PID제어 설계

임대영(Dae-Yeong Lim),박범석(Beom-Seok Park),정길도(kil-to Chong),김영철(Young-Chul Kim) 대한전자공학회 2010 대한전자공학회 학술대회 Vol.2010 No.6

쇠별꽃 메탄올추출물의 항산화능 및 B16F10 세포에서 광보호 효능

전근영 ( Geun Yeong Jeon ),진방민 ( Bang Min Jin ),조용권 ( Yong Kweon Cho ),김영철 ( Young Chul Kim ) 대한미용학회(구 대한미용과학회) 2021 대한미용학회지 Vol.17 No.2

The aim of the current study was to investigate the antioxidant ability of Stellaria aquatica L. methanol extract (SAME) and its photoprotection efficacy of B16F10 cells irradiated with UVA. Total polyphenol and flavonoid contents in SAME were estimated to be 284.4 TAE mg/g and 210.5 RE mg/g, respectively. At 1,000 μg/mL, the electron-donating ability of SAME was found to be 58.0% and its SC50 for free radical scavenging was 748.2 μg/ mL. The viabilities of B16F10 cells treated with SAME at 25 and 50 μg/mL were 87.7% and 73.1%, respectively. On the other hand, the viabilities of B16F10 cells treated with IBMX at 25 and 50 μg/mL were 81.3% and 76.1%, respectively. The maximum permissible levels for treating the B16F10 cells with SAME and IBMX were calculated to be 25 μg/mL. Irradiation with 5 and 10 mJ/㎠ UVA decreased the viabilities of the B16F10 cells by 21.6% and 25.1%, respectively, which, however, increased viabilities by 13.0% (p < 0.001) and 11.5% (p < 0.001), respectively on treatment with 25 μg/mL SAME. On the other hand, when 5 and 10 mJ/㎠ UVA-irradiated B16F10 cells was treated with 25 μg/mL IBMX, the viabilities increased by 8.7% (p < 0.05) and 4.7% (p < 0.01), respectively. These results suggest that SAME can be used as a natural cosmetic ingredient for photoprotection of melanocytes from UVA irradiation.

B16F10 세포에서 쇠별꽃 메탄올추출물의 멜라닌 생성 촉진 효능

전근영 ( Geun Yeong Jeon ),진방민 ( Bang Min Jin ),조용권 ( Yong Kweon Cho ),김영철 ( Young Chul Kim ) 대한미용학회 2021 대한미용학회지 Vol.17 No.3

The present study investigated into the efficacy of Stellaria aquatica L. methanol extract (SAME) on melanin production and photoprotection in B16F10 cells irradiated with or without UVA and elucidated the associated molecular and cellular mechanisms. The melanin content of B16F10 cells treated with 25 μg/mL of SAME and 3-isobutyl-1-methylxanthine (IBMX) was found to increase by 18.0% and 37.2%, respectively. On the other hand, tyrosinase activities of B16F10 cells treated with 1,000 μg/mL of SAME and IBMX increased by 35.2% and 27.8%, respectively. Additionally, the levels of reactive oxygen species (ROS) in B16F10 cells irradiated with 5 mJ/㎠ UVA increased by 66.1% (p<0.001). By contrast, when 5 mJ/㎠ UVA-irradiated B16F10 cells were treated with 25 μg/ mL SAME and IBMX the ROS production decreased by 10.8% (p<0.001) and 10.4% (p<0.001), respectively. Taken together, the melanin production-promoting and photoprotective efficacy of SAME in B16F10 cells appeared to be mediated through an increase in tyrosinase activity and a decrease in ROS production in these cells.

Nomoto모델을 이용한 선박의 선형 모델 분석 및 퍼지제어기 설계

임대영(Lim, Dae-Yeong),김영철(Kim, Young-Chul),정길도(Chong, Kil-To) 한국산학기술학회 2011 한국산학기술학회논문지 Vol.12 No.2

본 논문은 자동항로 추적(Track keeping control), 자동조타(Automatic steering), 자동 접이안(Automatic mooring control) 등으로 구성된 자동운항 시스템 중 자동조타장치의 성능 개선 알고리즘 개발에 대해 다루고 있다. 자동조타는 풍력 또는 조력 등의 영향으로부터 선박의 설정 항로와 실제 침로와의 차이를 계산하여 설정된 항로를 유지하며 항해하므로, 조타에 소요되는 선원의 지속적인 항해로 인한 운전 부담을 경감시키고 불필요한 타조작에 의 한 항로 이탈을 줄임으로써 항해거리 단축과 연료비용을 절약할 수 있는 시스템이다. 선박의 모델링을 위하여 Nomoto 모델에 근거하여 전달함수를 구하고, 조종성능(Manoeuvirng) 편리성을 고려하여 타각 입력에 대한 선수각 응 답으로 표시된 선박의 4자유도만을 고려한 선형 모델을 제안하고 선박 자동조타장치의 최대각과 타각율을 고려하여 Fuzzy제어기를 설계 하였고 PID제어기로 성능을 비교 분석하였다. This paper developed the algorithm for improving the performance the auto pilot in the autonomous vehicle system consisting of the Track keeping control, the Automatic steering, and the Automatic mooring control. The automatic steering is the control device that could save the voyage distance and cost of fuel by reducing the unnecessary burden of driving due to the continuous artificial navigation, and avoiding the route deviation. During the step of the ship autonomic navigation control, since the wind power or the tidal force could make the ship deviate from the fixed course, the automatic steering calculates the difference between actual sailing line and the set course to keep the ship sailing in the vicinity of intended course. first, we could get the transfer function for the modeling of ship according to the Nomoto model. Considering the maneuverability, we propose it as linear model with only 4 degree of freedoms to present the heading angle response to the input of rudder angle. In this paper, the model of ship is derived from the simplified Nomoto model. Since the proposed model considers the maximum angle and rudder rate of the ship auto pilot and also designs the Fuzzy controller based on existing PID controller, the performance of the steering machine is well improved.

DFT를 이용한 $\beta-FeSi_2$/Si (001) 에피택셜 계면 연구

황규철,김대희,오현철,임지혜,김영철,Hwang, Kyu-Cheol,Kim, Dae-Hee,Oh, Hyun-Chul,Rim, Ji-Hye,Kim, Yeong-Cheol 한국반도체디스플레이기술학회 2008 반도체디스플레이기술학회지 Vol.7 No.2

An epitaxial $\beta-FeSi_2$ structure on Si (001) substrate was calculated by using density functional theory (DFT). Unit cell of orthorhombic $\beta-FeSi_2$ and $\sqrt{2}\times\sqrt{2}\times2$ supercell were calculated to find the energetically favorable structures first. The $\chi$- and y-direction axes of $\beta-FeSi_2$ were changed into y- and z-direction axes to match its structure with that of Si, to minimize the lattice mismatch between $\beta-FeSi_2$ and Si. Distance between the Si (001) surface and the $\beta-FeSi_2$ surface was varied to find an optimum distance between them, resulting in 0.825 $\AA$.

신경회로망과 PI제어기를 이용한 중수로 핵연료 교체 로봇의 구동압력 제어

임대영(Lim, Dae-Yeong),이창구(Lee, Chang-Goo),김영백(Kim, Young-Baik),김영철(Kim, Young-Chul),정길도(Chong, Kil-To) 한국산학기술학회 2012 한국산학기술학회논문지 Vol.13 No.3

현재 가동 중인 월성 원자력 발전의 핵연료 교체로봇 시스템을 살펴보면 핵연료 교환에 필요한 구동압력 제 어를 위해 PI제어기를 사용한다. PI제어는 구조가 간단하고 이득 설정을 통해 시스템 요구조건에 만족하는 제어 성능 을 낼 수 있지만 밸브와 관로 등의 파라미터 변화로부터 적절한 이득 변경 없이 안정한 제어가 힘들다. 이러한 문제 를 해결하기 위해 PI제어기 이득을 동적으로 변경 하거나 PI제어기 출력을 보상하도록 제어기를 구성하는 것이 바람 직하다. 본 연구개발의 목적은 파라미터 변화에도 안정한 제어가 가능하도록 제어기를 설계하여 오차와 진동현상을 줄이는데 있다. 제안한 PI/NN제어 기법은 PI제어기와 신경회로망 제어기를 병렬 결합한 구조로 신경회로망 제어기가 PI제어기 출력을 보상하여 파라미터 변화에 강인하도록 설계 하였다. 제어기의 성능평가를 위해 직접 실 공정에 테스 트하기가 힘들기 때문에 공정의 특성을 반영하여 모델링한 시뮬레이터를 개발하였고, 시뮬레이션 결과를 실 공정데이 터와 비교하여 공정 특성을 모사함을 보였으며, 파라미터 변화에 PI/NN제어기가 오차 및 진동현상을 줄이는 것을 확 인 하였다. 또한, 실 공정에서 사용 중인 PI제어기를 주 제어기로 사용하면서 파라미터 변화에 대한 비선형성을 보상 하는 제어기 역할을 하기 때문에 신경회로망을 단독으로 사용하였을 때 보다 더 신뢰성 있고 안정적인 제어가 가능 하다. Look into the nuclear power plant of Wolsong currently, it is controlled in order to required operating pressure with PI controller. PI controller has a simple structure and satisfy design requirements to gain setting. However, It is difficult to control without changing the gain from produce changes in parameters such as loss of the valves and the pipes. To solve these problems, the dynamic change of the PI controller gain, or to compensate for the PI controller output is desirable to configure the controller. The aim of this research and development in the parameter variations can be controlled to a stable controller design which is reduced an error and a vibration. Proposed PI/NN control techniques is the PI controller and the neural network controller that combines a parallel and the neural network controller part is compensated output of the controller for changes in the parameters were designed to be robust. To directly evaluate the controller performance can be difficult to test in real processes to reflect the characteristics of the process. Therefore, we develope the simulator model using the real process data and simulation results when compared with the simulated process characteristics that showed changes in the parameters. As a result the PI/NN controller error and was confirmed to reduce vibrations.

복강내 종양으로 오인된 외상성 혈종: 증례보고

박종민 ( Jong Min Park ),김성엽 ( Seong Yup Kim ),정일용 ( Il Yong Chung ),김우식 ( Woo Shik Kim ),신용철 ( Yong Chul Shin ),김영철 ( Yeong Cheol Kim ),박세혁 ( Sei Hyeog Park ) 대한외상학회 2013 大韓外傷學會誌 Vol.26 No.4

Blunt abdominal trauma is commonly encountered in the emergency department. The lack of historical data and the presence of distracting injuries or altered mental status, from head injury or intoxication, can make these injuries difficult to diagnose and manage. We experienced a case of traumatic organized hematoma misdiagnosed as intra-peritoneal tumor with intestinal obstruction. A 52-year-old homeless male patient who have chronic alcoholism was admitted via emergency room with infra-umbilical abdominal pain. At admission, he was drunken status and so we could not be aware of blows to the abdomen. He had a unknown large operation scar on mid abdomen. A computed tomography (CT) scan showed the intestinal obstruction of the ileum level with 5.5cm sized mesenteric tumor. We performed adhesiolysis and widely segmental resection of small bowel including tumor with side-to-side anastomosis due to great discrepancy in size. He stated later that he was a victim of the violence before 3 weeks. A final pathologic report revealed well encapsulated, traumatic mesenteric hematoma with organizing thrombi, ischemia and abscess formation with multiple adhesion bands. Finally, the patient was discharged without complications on postoperative day 14.