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이헌,김정현,김승수,Lee, Hun,Kim, Jeong-Hyun,Kim, Soung-Soo 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.4
LPS와 PMA를 다양하게 조합하여 사람 단핵구 세포를 처리해서 이들로부터 사람 종양괴사인자가 생성되었다. 특히 사람 단핵구 세포 ($2{\times}10^7$ 세포/ml)를 PMA (10 ng/ml)로 먼저 6시간 처리하고 LPS $(20{\mu}g/ml)$로 나중에 6시간 처리하였을 때 가장 높은 종양괴사인자 활성을 얻을 수 있었다. 유도한 종양괴사인자는 혈청이 포함되지 않은 배양배지로부터 DEAE-Sephacel 이온교환 크로마토그라피, Sephadex G-75 gel filtration, SDS-PAGE 용출방법에 의해 정제하였다. SDS-PAGE 방법에 의해 결정된 종양괴사인자의 분자량은 대략 17,800 정도였고 Sephadex G-150 gel filtration 방법에 의해 얻은 native 분자량은 68,000 정도였다. 반면에 ${\lambda}gt11$ 발현 운반체 system에 의해 E. coli에서 발현된 recombinant 사람 종양괴사인자의 분자량은 Western blot 분석과 Sephadex G-150 gel filtration 방법에 의해 모두 17,400 정도로 나타났다. 이 같은 결과들로 보아 종양괴사인자의 monomer 형태와 multimer 형태가 모두 생물학적으로 활성을 갖는다는 것을 알 수 있다. Human tumor necrosis factor(TNF) was produced from human peripheral monocytes by treatment of the cells with various combinations of lipopolysaccharide(LPS) and phorbol myristate acetate(PMA). The best induction of TNF activity could be achieved when human peripheral monocytes ($2{\times}10^7$ cells/ml) were first treated with 10 ng/ml of PMA for 6 hours followed by 6 hours treatment with LPS$(20{\mu}g/ml)$. Induced TNF from serum-free culture media was purified by DEAE-Sephacel ion exchange chromatography, Sephadex G-75 gel filtration and SDS-PAGE elution. The molecular weight of the TNF as determined by SDS-PAGE was about 17,800, while the native molecular weight obtained by Sephadex G-150 gel filtration was 68,000. On the other hand, the molecular weight of recombiant human TNF obtained in E. coli by ${\lambda}gt11$ expression vector system was estimated to be 17,400 by Western blot analysis and Sephadex G-150 gel filtration. These results suggest that both monomeric and multimeric forms of TNF are biologically active.
사람 단핵구 종양괴사인자의 유도 , 정제 . 크로닝 및 발현
이헌,김정현,김승수 ( Hun Lee,Jeong Hyun Kim,Soung Soo Kim ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.4
The gene encoding subtilisin Carlsberg from Bacillus licheniformis has been isolated by the $quot;in-situ colony hybridization technique$quot; using synthetic oligonucleotide as a probe. Pstl digested chromosomal DNA was ligated into the Pstl site of pBR322. The ligation mixture was used to transform E. coli and the transformants were screened by the in-situ colony hybridization technique. Out of 3,500 colonies screened, one colony was hyridized to the probe. The recombinant DNA of this positive clone was named pS113. Digestion of pS113 by restriction endonucleases showed that the size of the inserted DNA was about 4.6 Kb and the restriction map was nearly like that of Jacobs`. To express the gene in Bacillus, Pstl digested pS113 was ligated into the Pstl site of pMK4. The ligation mixture was used to transform B. licheniformis and the transformants were assayed on casein plate for protease secretion. We obtained a clony having a distinctively large halo.
Thermogravimetric characteristics and pyrolysis kinetics of Giheung Respia sewage sludge
Mohd Roslee Othman,김승수,Young-Hun Park,Thanh An Ngo,김진수,Kwang Seok Lee 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.1
Sewage sludge acquired from Giheung Respia treatment facility was characterized and converted into gas,bio-oil and char by pyrolysis. The rate of conversion as a function of temperature was obtained from differential thermogravimetric analysis (DTG) for different heating rates. The activation energy calculated from data selected conversions shows that the activation energy decreased with increasing conversion up to 10%, steadily increased from 10 to 70%, and substantially increased from 70 to 90%. Depending on the level of conversion, the values of activation energies varied between 181 and 659 kJ/mol. The gas product obtained in the experiment at 450 oC, 20 min mainly included CO2 (30%), CO (23%) and CH4 (17%). The product yields of gas, oil and char were systematically studied by changing the pyrolysis temperature and residence time.
선천성 Long QT 증후군 환자의 경흉부 교감신경절제술을 위한 마취관리
김두식,김승수,이석우,김세환,장태호,박세훈,류시정,김경한 대한마취과학회 2000 Korean Journal of Anesthesiology Vol.39 No.3
A 23-year-old female patient was diagnosed with congenital long QT syndrome, discovered when she visited our cardiac department due to chest discomfort, because she had a prolonged QTc interval on ECG, history of syncopal attacks and seizures several times every year, and ingestion of anticonvulsants for several yeam. It is well known that the long QT syndrome is associated with sudden death secondary to ventricular tachyarrhythmia or fibrillation at a young age. Moreover cardiac artests during induction, maintenance, and awakening of anesthesia of these patients have been reported, and may be due to asymmetrical adrenergic stimuli in the heart, especially in the unrecognised case. This case report describes the anesthetic management of a patient with congenital long QT syndrome, who presented for cervicothoracic sympathectomy for a more permanent control of life-threatening ventricular arrhytbmias, and reviews the related literature.