우리나라 재배지역별 야콘 생산성 및 기능성 프락토올리고당 평가

김수정,손황배,홍수영,남정환,장동칠,김기덕,서종택,구본철,김율호,Kim, Su Jeong,Sohn, Hwang Bae,Hong, Su Young,Nam, Jung Hwan,Chang, Dong Chil,Kim, Ki Deog,Suh, Jong Taek,Koo, Bon Cheol,Kim, Yul Ho 한국작물학회 2017 한국작물학회지 Vol.62 No.3

The purpose of this study was to investigate the effects of envrionmental factors on the ecological responses, yield, and quality properties and physicochemical characteristics of yacon cultivated in various regions of Korea. Experiments were carried out in eight regions from 2010 to 2013. The temperature range in Jinbu, Bonghwa, Cheolwon, and Gangneung during the growth period of yacon cultivation was $17.5-24.6^{\circ}C$. The total yield and marketable yield of tuberous root in Jinbu were 4,065 and 3,196 kg/10a, respectively. The sugar content of yacon tuberous roots comprised 0.11-0.20% fructose, 0.11-0.37% glucose, 0.39-0.68% sucrose, 0.07-0.37% reducing sugar, and 7.03-9.62% fructo-oligosaccharides. The content of fructo-oligosaccharides, which is a functional substance, was the highest in yacon cultivated in Jinbu. Based on the productivity and functional fructo-oligosaccharides, the optimum areas to cultivate yacon are Jinbu and Bonghwa, which are located in the middle-highland zone (500-560 m) and have a suitable growth temperature of $18-25^{\circ}C$. The results of the present study indicate that temperature could be the factor with the greatest influence on the root growth of yacon.

프락토올리고당을 함유한 야콘 추출물의 비피더스균에 대한 잠재적인 프리바이오틱 효과

김수정(Su Jeong Kim),손황배(Hwang Bae Sohn),권빈(Bin Kwon),박명수(Myeong Soo Park),홍수영(Su Young Hong),남정환(Jeong Hwan Nam),서종택(Jong Taek Suh),이종남(Jong Nam Lee),김기덕(Ki Deog Kim),장동칠(Dong Chil Chang),김율호(Yul Ho Kim 한국식품과학회 2020 한국식품과학회지 Vol.52 No.2

본 연구에서는 야콘 덩이뿌리 추출물이 프로바이오틱스인 비피더스균과 락토바실러스균의 생장에 미치는 영향을 구명하고자 하였다. 야콘의 당함량을 HPLC로 분석한 결과, 생체 중 100 g 당프락토올리고당 함량이 7.60 g으로 가장 많았으며, 유리당은 수크로스가 0.72 g, 프럭토스 0.34 g, 글루코스 0.26 g 순의 함량을 보였다. 야콘 추출물이 장내 유산균 활성에 미치는 영향을 구명하기 위해 락토바실러스 3종(L. acidophilus AD031, L. plantarum BH02 and L. fermentum BH03)과 비피더스 3종(B. bifidum BGN4, B. longum BORI, B. lactis AD011) 등 6종을 대상으로 대조군과 야콘처리군 배지에서 유산균의 생장속도를 비교하였다. 락토바실러스 3종의 균주들은 대조군과 야콘처리군에서 대수기에 도달하는 시간이 비슷하였고, 생균수에서도 비슷하거나 감소하는 경향을 보였다. 하지만, 비피더스 3종의 경우 야콘처리군에서 모두 생장이 증가되었고, 특히, B. longum BORI 균주는 배양10시간 이후부터 대조군과의 확연한 차이를 나타냈다. 이와 같은 결과로, 야콘 추출물은 장내 유익균 중에서도 비피더스균에 특이적인 증식 효과를 보이며, 야콘의 프락토올리고당이 비피더스균 증식에 적합한 영양원임을 확인하였다. This study was conducted to investigate the potential prebiotic activity of an extract from yacon tuberous root on the growth of probiotic strains Bifidobacterium and Lactobacillus. Results showed that the amount of fructooligosaccharides per 100 g fresh root was the highest (7.60 g), followed by sucrose (0.72 g), fructose (0.34 g), and glucose (0.26 g). The in vitro culture test of intestinal beneficial bacteria, including Bifidobacterium longum BORI, B. bifidum BGN4, and B. lactis AD011 showed effective growth on the MRS-Yacon medium containing yacon extract, whereas the growth of Lactobacillus acidophilus AD031, L. plantarum BH02, and L. fermentum BH03 did not differ from that of the control groups. In particular, B. longum BORI showed better growth than the control group after 10 h of incubation. These results indicate that yacon can be a natural prebiotic source of fructooligosaccharides, which can exert a prebiotic effect on intestinal microflora by selectively enriching Bifidobacterium.

‘께서, 에서, 서’의 문법 기능에 대하여

김수정(Kim, Su-Jeong),정경옥(Jung, Kyung-Ok),서종학(Suh, Jong-Hak) 한국어문학회 2014 語文學 Vol.0 No.126

The purpose of this study lies in clarifying the grammatical function of Korean particles ‘kkeseo, eseo, seo(께서, 에서, 서)’ and establishing their grammatical position. In addition to their features discovered in a number of discussions, it is claimed that through the analysis of their syntactic structure and passive sentences, ‘kkeseo, eseo, seo(께서, 에서, 서)’ are revealed to play a role of the subjects of noun phrase. However, ‘kkeseo, eseo, seo(께서, 에서, 서)’ are defined as auxiliary particles rather than nominative particles.

국내 수집 자생화의 고랭지 생육특성과 적응성 평가

김수정(Su Jeong Kim),안순영(Soonyoung Ahn),서종택(Jong Taek Suh),이창희(Chang Hee Lee),김기선(Ki Sun Kim) 한국원예학회 2009 한국원예학회 학술발표요지 Vol.2009 No.10

전처리제가 절화 아이리스의 수명, 안토시아닌 함량과 peroxidase 활성에 미치는 영향

김수정(Su-Jeong Kim),남춘우(Chun-Woo Nam),서종택(Jong-Taek Suh),유동림(Dong-Lim Yoo),김기선(Ki-Sun Kim) 한국원예학회 2005 원예과학기술지 Vol.23 No.2

Pre-treatment solutions on vase life, water content, water uptake, anthocyanin content, and peroxidase activity of Iris hollandica ‘Blue Magic’ were investigated. The vase life of cut Iris treated with 3% sucrose plus 100 ㎎ㆍL?¹ benzyl amino-purine (Sucrose + BA) was extended 4 days longer than the control. The treatments of 3% sucrose plus 0.4 mM silver thiosulphate (Sucrose + STS) and 3% sucrose plus 200 ㎎ㆍL?¹ 8-hydroxyquinoline sulphate (Sucrose + HQS) did not markedly extend the vase life. The amounts of water uptake and transpiration by all the treatments decreased at 2 to 4 days after harvest, but the amounts of Sucrose + BA were higher than that of the control. The water balance of Sucrose + BA showed slower change to minus value than that of the control. Anthocyanin content in the flower petal treated with Sucrose + BA was 3.5 times higher than that the control. The Sucrose + BA treatment delayed petal discoloration and senescence. Peroxidase (POD) activities of all the treatments reached the maximum stage at the 4th day after the treatments and decreased thereafter. Sucrose + BA treatment showed the highest POD activity at the 4th day.

자주꽃방망이 지대별 환경조건에 따른 화색발현 특성

김수정(Su Jeong Kim),남춘우(Chun Woo Nam),유동림(Dong Lim Yoo),서종택(Jong Taek Suh),염옥희(Ok Hee Yeum),조명래(Myoung Rae Cho),Yu Ji Lian 한국원예학회 2005 한국원예학회 학술발표요지 Vol.2005 No.10

중등교과서의 대명사 관련 교육내용 분석 연구

김수정(Kim Su-jeong),서종학(Suh, Jong-hak) 국어교육학회 2012 국어교육연구 Vol.50 No.-

This thesis was written to analyze the educational content for teaching pronouns in the secondary school curriculum. The topic is currently debated in academia. In this research, we studied educational contents of pronouns as follows. First, we studied educational contents of pronouns in the revised curriculum of 2007, 23 textbooks on 『국어(Korean)』 and 『생활국어(Practical Korean)』 for middle school students. Second, we studied educational contents of pronouns in the revised curriculum of 2009, and 4 Korean text books on 『독서와 문법(Reading and Grammar)』 for high school optional curriculum. And finally, we analyzed the problems of educational contents and proposed an alternative point. According to textbooks, the most general notion of a pronoun is "the words which substitute for names of persons, things and places." Examples of most frequently used pronouns are, "나(I), 너(you), 이것(this), 그것(it), 저것(that), 여기(here), 저기(there)". We researched the content relevance of teaching Korean grammar while focusing on the notion of pronouns, the example vocabulary and activities related to pronouns. We also researched how pronouns were classified by textbooks. Textbooks have features that should convey the most essential core information to middle and high school students using limited space and materials. Therefore, the notion of pronouns, the example vocabulary and activities related to pronouns should work efficiently within limited space. Investigations recently made on textbooks based on the revised curriculum of 2011 are currently at their peak. Pronouns will be dealt with as a subcategory of content related to the parts of speech in the revised curriculum of 2011. We expect our arguments will make many aware of the content problems in teaching pronouns and influence others to seek alternatives.

통합세척시스템 활용시 포장재 종류별 세척감자의 품질 특성

김수정(Su Jeong Kim),손황배(Hwang Bae Sohn),Manjulata Mekapogu,권오근(Oh Keun Kwon),홍수영(Su Young Hong),남정환(Jung Hwan Nam),진용익(Yong Ik Jin),장동칠(Dong Chil Chang),서종택(Jong Taek Suh),정진철(Jin Cheol Jeong),김율호(Yul H 한국식품과학회 2016 한국식품과학회지 Vol.48 No.3

우리나라에서 가장 많이 유통되고 있는 감자 품종인 ‘수미’를 사용하여 세척감자의 실용화를 위한 적정 포장재를 개발하고자 본 연구를 수행하였다. 세척감자의 포장재에 따른 품질특성의 변화를 구명하기 위해 무포장(NP, positive control), 종이봉투(PB, negative control), 양파망(ON), 무공투명비닐백(TP), 유공불투명비닐백(OP) 총 5종의 포장재를 대상으로 Hunter 값, 색차값(ΔE value), 총 엽록소 및 글리코알카로이드(PGA) 함량 등 품질 특성을 평가하였다. 그 결과, Hunter a값(적색도/녹색도)은 세척감자 저장 12일째에 양파망(ON)과 무공투명비닐백(TP)에서 −a값을 나타내 녹색도로 바뀌었다. 색차값은 무포장(NP)에서 높게 나타나 다른 포장재에 비해 녹화가 심했다. 총 엽록소 함량은 저장기간에 따라 뚜렷하게 증가하는 경향을 보였으며, 무포장에서 저장 15일째 가장 큰 증가를 보였다. 세척감자의 괴경 내피 부위의 PGA 함량은 모든 처리에서 저장 15일째 5 mg/100 g 이하로 낮았으며, 외피 부위에서도 4.5-9.3 mg/100 g으로 국제연합식량농업기구(FAO) 권장기준(20 mg/100 g)보다 낮았다. 녹화와 각 품질평가 요인의 상관성을 구명하기 위해, 피어슨 상관분석을 한 결과, 엽록소 함량은 색차값(0.90786)과 높은 정의 상관관계를 보였으나, Hunter a값은 색차값(−0.86021), 엽록소 함량(−0.85196)과는 높은 부의 상관관계를 보였다. 글리코알카로이드(PGA)와 녹색도 품질평가 요인과는 상관관계가 없는 것으로 나타났다. 이러한 엽록소 함량, 색도 색차와 PGA 함량 등의 결과를 바탕으로 녹화와 소비자 선호도를 고려할 때 평가항목에서 우수한 유공불투명포장재가 세척감자 소포장재로 유망할 것으로 판단되었다. This study was performed to investigate the effect of packaging materials on quality characteristics of washed potatoes such as Hunter’s a value, chlorophyll and potato glycoalkaloids (PGA) content during their storage for 15 days. Packaging methods were evaluated into five ways: no packaging (NP, positive control), paper bag (PB, negative control), onion net (ON), transparent oriented to polypropylene without hole (TP), opaque oriented polypropylene with 4 holes (OP). Hunter’s a values of washed potatoes showed minus in NP and TP after 12 days storage, whereas all plus values were observed in those of PB, ON, and OP. Total chlorophyll content of washed potatoes was the highest in no packaging at 15 days after storage. The PGA content of washed potatoes showed low levels in flesh part (below 5 mg/100 g · FW) as well as in peel part (4.5-9.3 mg/100 mg · FW) in all packagings up to 15 days after storage.

실내 적응 분화용 자생화 선발 및 적정 광 조건

김수정(Su-Jeong Kim),남춘우(Chun-Woo Nam),유동림(Dong-Lim Yoo),서종택(Jong-Taek Suh),류승열(Seung-Yeol Ryu) 한국원예학회 2001 한국원예학회 학술발표요지 Vol.2001 No.5

쓴메밀 종자의 추출방법에 따른 루틴 및 퀘세틴 함량 비교

김수정(Su Jeong Kim),손황배(Hwang Bae Sohn),김금희(Geum Hee Kim),이유영(Yu Young Lee),홍수영(Su Young Hong),김기덕(Ki Deog Kim),남정환(Jeong Hwan Nam),장동칠(Dong Chil Chang),서종택(Jong Taek Suh),구본철(Bon Cheol Koo),김율호(Yul Ho 한국식품과학회 2017 한국식품과학회지 Vol.49 No.3

본 연구는 초고성능액체크로마토그래피(Ultra Performance Liquid Chromatography, UPLC)를 이용하여 쓴메밀 종자에서 루틴 및 퀘세틴 함량을 신속 정확히 평가하기 위한 효율적인 추출방법을 구명하고자 수행하였다. 추출방법으로 환류냉각추출, 초음파추출, 상온교반추출, 초음파후 환류냉각추출, 상온교반 후 환류냉각추출, 상온교반후 초음파추출, 상온교반과 초음파 후 환류냉각추출 등 7가지 방법을 비교하였다. 루틴 함량에서 7가지 추출방법은 통계적으로 유의한 차이를 보였으나, 퀘세틴 함량에서 추출방법 간 유의차가 없었다. 환류냉각추출의 루틴과 퀘세틴 함량은 각각 2,277, 158 mg/100 g으로 추출방법 중 함량이 가장 많아 추출수율이 높았으며 검출횟수도 가장 높았다. 반면, 초음파 추출의 루틴과 퀘세틴의 함량이 처리구 중 가장 낮았으며, 검출횟수도 가장 적었다. 환류냉각추출에서 일내 반복성(intra-day repeatablility)과 일간 정밀성(inter-day precision)은 상대적표준편차(relative standard deviation, RSD)가 0.4-3.2%로 안정적으로 추출되었고, 정확도는 88.8-102.4%로 국제적 회수율 권고치 기준에 부합하였다. 따라서, 쓴메밀 루틴 추출방법으로는 추출 소요시간이 1시간 이내로 짧으면서 루틴 함량이 가장 안정적으로 많이 추출되는 환류냉각추출이 가장 적합하였다. The stability and accuracy of ultra-performance liquid chromatography (UPLC) used for evaluating the contents of rutin and quercetin in tartary buckwheat (Fagopyrum tataricum Gaertn.) seeds extracted by seven different extraction methods were determined. The seven extraction methods were reflux extraction (RE), ultra-sonification extraction (UE), stirrer extraction (SE), RE after UE (UE+RE), RE after SE (SE+RE), UE after SE (SE+UE), and RE with UE after SE (SE+UE+RE). Among the seven extraction methods used, RE yielded comparatively higher contents of rutin (2,277 mg/100 g) and quercetin (158 mg/100 g) than those yielded by other six extraction methods. The intra-day repeatability and inter-day precision of RE was 0.4-3.2% considering relative standard deviation (RSD), while accuracy was 88.8-102.4%. Therefore, RE with UPLC would be a rapid, accurate, and stable method for analyzing rutin and quercetin contents in tartary buckwheat.