Monascus anka 의 균주선발 및 색소생성 조건

김수언,김정구 한국농화학회 1990 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.3

Monascus anka strains with higher pigment production were developed using UV mutation and natural selection. To obtain organic solvent soluble pigments from Manascus anka, the following culture conditions were compared; standing and shaking culture with Nishikawa's medium, and shaking culture with Lin's medium. Shaking culture in Lin's medium exhibited decrease in solvent.-soluble intracellular pigments after initial increase. The decrease was accompanied by the increase in water-soluble extracellular pigments. Manascus anka preferred sucrose and ethanol among 7 carbon sources tested. Treatment of sterol biosynthesis inhibitors, (±)-miconazole and chlorocholine chloride(CCC), directed carbon pool to the biosynthetic pathway leading to the pigments with CCC's more pronounced effect. Two dimensional TLC revealed at least 7 yellow pigments suggesting existence of hereto unreported pigment. One of the most abundant yellow pigments was isolated and found to be ankaflavin by NMR and MS analysis.

쪽(Polygonum tinctorium) 조직배양을 이용한 인디고와 인디루빈 유도체 합성

김수언 서울대학교 농업생명과학대학 농업개발연구소 1998 농업생명과학연구 Vol.2 No.-

Indoles (5-bromo-, 5-fluoro-, 5-chloro-, 5-hydroxy-, 4-methyl-, 5-methyl-, 6-methyl-, and 7-methylindole) were fed at 1 mM for 10 to 21 days into the tissue cultures of Polygonum tinctorium including cell suspension, root and shoot cultures. They were converted into the corresponding indirubin and indigo derivatives with the exception of 5-hydroxyindole. The yield was ranging 0.7~1.1 mg/g dried weight for indigos and 0.007~0.024 mg/g dried weight for indirubins. Presence of the corresponding indican derivatives in the shoot culture suggested that the pigment production proceeded through the indican formation.

11,12 - Dihydroarteannuic acid 에서 artemisinin 의 생합성

김수언,김남철 한국농화학회 1992 Applied Biological Chemistry (Appl Biol Chem) Vol.35 No.2

To investigate the biosynthetic pathway leading to artemisinin, the putative precursors, arteannuic acid and 11,12-dihydroarteannuic acid, were incubated in a cell-free system. For the experiment with dihydroarteannuic acid, artemisinin was generated in tumor homogenate. These results showed that dihydroarteannuic acid could be converted to artemisinin enzymatically. However, the experimental condition failed to convert arteannuic acid into 11,12-dihydroarteannuic acid(Received February 20, 1992, accepted March 31, 1992).

Artemisia annua L . 의 조직배양을 이용한 이차대사 산물의 생산

김수언,김정구,김남철,임형준,한대룡 한국농화학회 1992 Applied Biological Chemistry (Appl Biol Chem) Vol.35 No.2

Artemisia annua contains the antimalarial principle, artemisinin. The possibility of the production of this compound through tissue culture technique was studied. The optimum combinations of hormones for the induction of callus were ρ-chlorophenoxyacetic acid(pcPA) and 6-benzylaminopurine(BAP) or pcPA and N-isopentenylaminopurine(2iP) in 0.05 ㎎/ℓ each. For the growth of callus, the same combination of pcPA and BAP was optimum in concentrations of 1.0μM and 0.5μM, respectively, and the optimal concentration of sucrose was also found to be 2%(w/v). Tissue culture from the crown gall grew faster than normal callus. In the suspension culture broth and the cells of normal callus or Agrobacterium-transformed tumors, arteannuic acid and 11,12-dihydroarteannuic acid were found together with common phyfosterols, whereas artemisinin was not found(Received February 20, 1992, accepted March 31, 1922).

항공사 SNS 마케팅활동의 속성이 브랜드 태도 및 행동 의도에 미치는 영향 : 브랜드 태도의 매개효과를 중심으로

김수언,최호규 국립공주대학교 KNU 기업경영연구소 2024 기업경영리뷰 Vol.15 No.2

With the recent revitalization of smartphones, the use of SNS is becoming limitless, and airlines are also utilizing it as a useful marketing channel. Therefore, this study was conducted to determine the influence of the attributes of airline SNS marketing activities on brand attitudes and consumers' actual behavioral intentions. A survey was conducted among domestic airline SNS users, and a total of 216 questionnaires were used for the analysis. The results of the analysis showed that, first, among the attributes of airline SNS marketing activities, informativeness and playfulness have a positive effect on brand attitude. Second, brand attitude has a positive effect on behavioral intention, i.e., intention to use and intention to recommend. Third, among the attributes of airline SNS marketing activities, trustworthiness has a positive effect on behavioral intention to use and recommendation intention. Finally, brand attitude was found to fully mediate the relationship between airline SNS marketing activity attributes and intention to use and intention to recommend. This confirms that airline SNS marketing attributes are an effective way to increase positive thoughts about the brand and increase ticket purchasing power. Airlines with better SNS marketing tools are able to create closer relationships with their customers, which in turn generates higher revenues. This suggests that airlines need a differentiated SNS marketing strategy that promotes effective communication using social media.

Simplified Model Predictive Control Method for Three-Phase Four-Leg Voltage Source Inverters

김수언,박소영,곽상신 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.6

A simplified model predictive control method is presented in this paper. This method is based on a future reference voltage vector for a three-phase four-leg voltage source inverter (VSI). Compared with the three-leg VSIs, the four-leg VSI increases the possible switching states from 8 to 16 owing to a fourth leg. Among the possible states, this should be considered in the model predictive control method for selecting an optimal state. The increased number of candidate switching states and the corresponding voltage vectors increase the calculation burden. The proposed technique can preselect 5 among the 16 possible voltage vectors produced by the three-phase four-leg voltage source inverters, based on the position of the future reference voltage vector. The discrete-time model of the future reference voltage vector is built to predict the future movement of the load currents, and its position is used to choose five preselected vectors at every sampling period. As a result, the proposed method can reduce calculation load by decreasing the candidate voltage vectors used in the cost function for the four-leg VSIs, while exhibiting the same performance as the conventional method. The effectiveness of the proposed method is demonstrated with simulation and experiment results.

Calibration of Detection System of Crack in Concrete Structure by Using Image Processing Technology

김수언,신성우,박정학,최만용 한국비파괴검사학회 2011 한국비파괴검사학회지 Vol.31 No.6

The investigation of concrete structure typically relies on visual inspection which is one of the basic inspection techniques. Image processing techniques play a crucial role in the growing field of automatic surface inspection technique. However, kinds of inspection equipment, environmental condition and detection algorithm have much influence on the reliability of inspection result. This paper proposes a verification method and testing procedure for the reliability of inspection results and surveys characteristics of image acquisition systems and crack inspection algorithms.

Claviceps sp . 에서 Cytochrome P - 450 및 NADPH - Cytochrome c Reductase 정제 및 그 특성

김수언,김인수,John A . Anderson ( Soo Un Kim,In Soo Kim,John A . Anderson ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.3

Microsomes of Claviceps purpurea PRL 1980 had a maximum concentration of cytochrome P-450 of 40 pmole per ㎎ protein. Cytochrome P-450 and NADPH-cytochrome c reductase were purified from C. purpurea PRL 1980 microsomes to concentrations of 421 pmole per ㎎ protein and 387 pmole per min per mg protein, respectively. Activity was not reconstituted from these fractions, but NADPH-cytochrome c reductase increased agroclavine hydroxylase activity of C. purpurea PRL 1980 microsomes. The activity was not stimulated by rat liver microsomal NADPH-cytochrome c reductase. Claviceps sp. SD58 microsomal agroclavine hydroxylase had V_(max) of 3.3 pkat per ㎎ and K_m of 0.11 mM for agroclavine. Agroclavine bound to cytochrome P-450 from Claviceps sp. SD58 to give a type II spectrum. The absorbance maximum in the CO-reduced vs reduced difference spectrum of purified cytochrome P-450 from C. purpurea PRL 1980 and Claviceps sp. SD58 was at 449 ㎚.

국제화 사업 추진 : 학회지 국제화 사업 추진 - SCI 등재 추진사업

김수언,임융호,이회선 한국응용생명화학회(구 한국농화학회) 2001 농업생명과학 Vol.8 No.1

Determination of Biosynthetic Pathway of Decursin in Hairy Root Culture of Angelica gigas

김수언,Xiuhong Ji,Bum Huh 한국응용생명화학회 2008 Journal of Applied Biological Chemistry (J. Appl. Vol.51 No.4

To establish the biosynthetic pathway of decursin in Angelica gigas Nakai, feeding experiment with stable isotope-labeled precursors were conducted. Umbelliferone and decursin were labeled with deuterium at C-3. The umbelliferone, the decursin, and other commercially available putative precursors, L-phenylalanine-ring-d5 and trans-cinnamic acid-d7, were fed to the hairy root culture of A. gigas. Each deuterated compound was incorporated into decursinol, decursinol angelate, and decursin as determined by mass spectrometric analysis. These findings confirmed the coumarin biosynthesis pathway sequence is composed of phenylalanine, cinnamic acid, umbelliferone, decursinol, and decursin.