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천연자원의 라디칼 소거능과 최종당화산물의 생성저해활성 검색
김민석,김동욱,류동영 한국생약학회 2006 생약학회지 Vol.37 No.4
oxygen species (ROS) and formation of advanced glycation end products (AGEs) play an important roleural resources which used to the treatment of diabetes mellitus in a folk remedy were investigated with an in vitro systememploying radical scavenging activity and inhibitory activity of AGEs formation. In results, the extracts of Aspalathus linearis,Rubus coreanus, Rosa rugosa, and Epimedium koreanum significantly inhibited the 1,1-diphenyl-2-picrylhydrazyl (DPPH) rad-ical with IC50 values less than 10g/ml. The extracts of Zea mays, Cucurbita moschata, Cudrania tricuspidata, and Aspalathuslinearis efectively reduced the formation of AGEs compared with the positive control N-acetyl-L-cystenine (NAC) and ami-noguanidine (AG). In addition, the extracts of Aspalathus linearis, Commelina communis, Cornus officinalis, and Lespodezacuneata showed the all inhibitory activity against DPPH radical and AGEs formation. Also, these resources definitely showedthe radical scavenging activity against peroxynitrite (ONOO) and hydroxyl radical (·OH) relating to high glucose-induced ROSproduction. Thus, these results suggest that some natural resources may regulate the initiation and progression of diabetic neph-ropathy through inhibition of ROS production and AGEs formation. Key wordsdiabetic nephropathy, reactive oxygen species (ROS), advanced glycation end products (AGEs), naturalresources .... .. (diabetic nephropathy). .... ...
cPCR 기법을 이용한 초기배양 pH에 의한 반추위 섬유소 분해 박테리아의 부착 및 발효에 관한 연구
김민석,성하균,김현진,이상석,장종수,하종규,Kim, M.S.,Sung, H.G.,Kim, H.J.,Lee, Sang-S.,Chang, J.S.,Ha, J.K. 한국축산학회 2005 한국축산학회지 Vol.47 No.4
본 연구는 배양초기 pH 조건이 F. succino- genes의 섬유소 부착과 섬유소 소화에 미치는 영향을 보고자 실시하였다. 선정된 specific primer를 이용하여 F. succinogenes의 genomic DNA로부터 445bp의 16S rDNA 절편을 증폭하여 205bp의 internal control을 제작하였고, cPCR 결과로부터 박테리아 수를 계산할 수 있는 표준곡선의 회귀식($r^2$>0.99)을 얻을 수 있었다. In vitro 배양초기 pH에 따른 F. succ- inogenes의 cellulose 부착을 cPCR로 모니터링한 결과, 발효과정 전 기간동안 초기 pH가 6.8과 6.2일 때 cellulose 건물 g당 부착 균주의 수가 pH 5.6일 때 보다 높았으나, pH 6.8과 6.2 사이에서는 큰 차이는 없었다(p>0.05). Cellulose 분해는 배양시간이 진행됨에 따라 증가 되었으며, 분해 정도는 pH가 증가함에 따라 더 높았다. 배양초기 pH가 6.8, 6.2 그리고 5.8일 때 48시간동안 감소한 pH는 각각 0.24, 0.58 그리고 0.16 이었다. 가스 생산량은 발효 시간이 경과함에 따라 pH가 높을수록 더 많았다. 결론적으로 발효 초기 pH는 F. succinogenes에 의한 cellulose 소화, 가스 생산, pH 변화 및 cellulose 부착에 큰 영향을 주었으며, 특히, 낮은 pH(5.8)에서는 섬유소 소화 및 박테리아 부착을 현저한 감소 시켰다. The cPCR technique was used to monitor rumen fermentation and attachment of Fibrobacter succinogenes to cellulose at different pH in the in vitro culture medium. The target fragments of 16S rDNA(445 bp) were amplified from genomic DNA of F. succinogenes with specific primers and internal controls(205 bp) were constructed. Cell counts were estimated from the amounts of genomic DNA, which was calculated from cPCR results. F. succinogenes in pH 6.8 and 6.2 showed apparently higher attachment than in pH 5.8 during all incubation time. There were some difference between pH 6.8 and 6.2 in the degree of attachment, but the different was not significant (P>0.05). Cellulose degradation increased in process of incubation time and the increasing rate was higher when initial pH was higher. The pH in culture medium decreased regardless of initial pH in course of incubation time. After 24 h of incubation, medium pH was dropped by 0.24, 0.58 and 0.16 units from original medium pH 6.8, 6.2 and 5.8, respectively. More gas was produced at higher initial pH in the same manner as in cellulose degradation. In summery, Initial pH of rumen culture in vitro significantly influenced cellulose digestion, gas production, pH change and bacterial attachment. Especially, low pH(5.8) resulted in much lower bacterial attachment and fiber digestion compared to higher medium pH.
김민석 한국근현대사학회 2016 한국 근현대사 연구 Vol.77 No.-
A cooperative is an organization which leads various business in cooperation with each other through the mutual aid of the economically challenged. After the farmland reform, the necessity of an agricultural cooperative was raised in farming areas as the number of small-scale independent farmers increased. In the late 1950s, the agricultural cooperative, and the agricultural bank were established separately. There was a discussion about the integration of these two institutions during the period of the Jang Myeon government, but it failed to be realized because of its short ruling period. Integration of the agricultural coop and the agricultural bank was resolutely carried out by the Park, Jeong-hui government after May 16 coup. The president of the central federation and cooperative representative of various levels in the re-organized agricultural cooperative were appointed by the government. The re-organized agricultural cooperative also relied more on the government for finance and projects that farmers. The Park, Jeong-hui government’s action was plainly the negation of the agricultural cooperative. The Ri-Dong cooperative having farmers as coop members became powerless, whereas the authority of the Si-Gun cooperatives and the National Agricultural Cooperative Federation, which carried out the government-commissioned projects and financial services, became excessively bulky. In the agricultural administration of the Park, Jeong-hui government in the 1960s, the agricultural coop achieved quantitative growth and organizational alignment, but it came to have the factors like a back-scratching alliance with the government, and the government’s direct strengthening of influence. Considering the then farming area situation, no one can deny the government’s aggressive role in the agricultural coop. Also, there were some unavoidable aspects in relation to appointments of discharged soldiers, or the former pubic officials by the government order. However, it is doubtful that the then government intervention had to be conducted definitely in a controlled way other than support. The agricultural coop made a success as the government’s control agency over rural society, but it failed as a cooperative. The failure of the agricultural coop means the disappearance of one of the possibilities that it could have become an efficient main agency of economic activity in the farming villages.