Distribution of Aquaporins (Water Channels) in the Rat Salivary Glands

정지연(Ji-Yeon Jung),한창룡(Chang-Ryoung Han),정연진(Yeon-Jin Jeong),오원만(Won-Mann Oh),김미원(Mi-Won Kim1),김선헌(Sun-Hun Kim),김옥준(Ok-Joon Kim),김현진(Huyn-Jin Kim),고정태(Jeong-Tae Koh),최홍란(Hong-Ran Choi),김원재(Won-Jae Kim) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.3

침샘은 하루 1.5 l의 침을 분비하며, 일반적으로 침샘에서 수분이동은 능동적 염분 이동에 따른 삼투현상에 의해 일어난다. 따라서 침샘에서 높은 수분투과성으로 인해 수분통로 단백인 aquaporin (AQP)들이 풍부하게 존재할 수 있다. 지금까지침샘에 4가지 형태의 AQP이 존재한다고 알려져 있지만 침샘에서 정확한 위치와 수분 이동에 대한 각 AQP들의 역할은 아직 확실치 않다. 본 연구는 300 g 정도의 흰쥐를 pentobarbital sodium (50 mg/kg, IP) 마취하에서 carbarchol (10 μg/kg)을 복강 내 투여한 후 AQP들의 역할과 분포를 면역조직화학방법으로 조사하였다. AQP1은 침샘의 미세혈관의 내피세포와 샘세포와 샘관 주위에 존재하는 근육상피세포에 존재하였다. AQP4는 침샘관의 마지막 부위인 배출관에 존재하였다. AQP5는 주로 장액세포의 바닥가쪽과 세포사이 분비소관을 포함한 세포꼭대기쪽막 에 존재하였으며 콜린성 분비 자극으로 세포꼭대기쪽막으로 이동되어 밀집되었다. AQP5는 모든 침샘의 사이관과 줄무늬 관에도 존재하였으며 점액세포는 약하게 존재하였다. AQP8은 AQP5처럼 장액세포의 바닥쪽막과 세포사이 분비소관을 포함한 세포꼭대기쪽막에 존재하였으며 콜린성 침분비 자극으로 세포꼭대기쪽막쪽으로 이동되어 밀집되었다. 이상의 실험결과는 AQP5와 AQP8이 장액세포에서 일차 침 형성시 일어나는 수분이동의 주된 통로임을 시사하였다. The salivary glands produce 1.5 l of fluid per day. As in other organs, the general paradigm in the salivary glands is that water movement occurs secondary to osmotic driving forces created by active salt transport. Therefore, high water permeability in salivary glands is expected to need a variety of aquaporin (AQP), a water channel. Although four AQPs have been known to reside in salivary glands, the precise location and roles of AQPs have been not well examined. This study is aimed to investigate the distribution of AQPs in 3 major salivary glands and their changes after cholinergic stimulation using immunohistochemical study in Sprague Dawley rats weighing 300 g under pentobarbital sodium anesthesia. AQP1 was localized in the endothelial cells of all salivary capillary vessels and the myoepithelial cells. AQP4 was demonstrated in the epithelium of the excretory ductal cells of all salivary glands. AQP5 and 8 were abundantly present in the basolateral membrane and apical membranes of the serous acini including intercellular secretory canaliculi, whereas AQP5 was weakly present in mucous acini. In addition, AQP5 was found in the epithelium of the intercalated and striated ducts. Upon stimulation of carbachol (10 μg/kg, I.P). AQP5 and 8 tended to translocate from basolateral membrane to the apical membrane, appearing as clusters of dots. These results suggest that AQP5 and 8 are the candidate molecules responsible for the water movement in salivary acinar cells.

임상연구 실태조사

김미원,Kim, Mi-Won 대한간호협회 2001 대한간호 Vol.40 No.4

오징어 먹물을 첨가한 Yellow Layer Cake 개발에 관한 연구

김미원 ( Mi Won Kim ),김애정 ( Ae Jung Kim ),임영희 ( Young Hee Lim ),김명희 ( Moung Hee Kim ) 한국식품영양학회 2007 韓國食品營養學會誌 Vol.20 No.3

This study was performed to develop a functional yellow layer cake with added cuttlefish ink, by evaluating its physiochemical properties and sensory qualities. Viscosity tended to increase, and height decrease, with the addition of cuttlefish ink. The sensory evaluation revealed the addition of cuttlefish ink in the cake. Specifically, at the 0.3% level color, flavor, and gumminess were enhanced. The brightness(L) of the crumb was highest in the in the control group, at 72.76, and decreased with increasing amounts of cuttlefish ink. The crust of the yellow layer cake was highest in the control group. Furthermore, redness(a) of the crumb was lowest in the control group. but for the crust, it was highest in the control group at crust. Yellowness(b) decreased with increasing amounts of cuttlefish ink for both the crumb and crust. The hardness, cohesiveness, gumminess, and chewiness of the yellow layer cake was highest in the control group.

세라밴드를 이용한 운동프로그램이 여성 노인의 신체구성, 혈압 및 체력에 미치는 효과

김창숙(Kim Chang-Sook),박인혜(Park In-Hyae),김미원(Kim Miwon),장숙희(Jang Sook-Hee),김영재(Kim Young-Jae),박명희(Park Myung-Hee),김옥(Kim Ok),선향송(Sun Hyang-Song) 대한근관절건강학회 2007 근관절건강학회지 Vol.14 No.2

Purpose: The purpose of this study was to determine the effects of exercise using Thera Band on body compositions, blood pressure and physical fitness in the elderly women. Method: The study was designed as a nonequivalent control group pretest-posttest quasi-experimental study. Twenty four subjects were assigned for experimental group and twenty one subjects were assigned for control group. The experimental group participated in Thera Band exercise program consisting of 60 minutes per session, 3 times a week during the period of 12 weeks. The effects of the program were assessed using the instruments measuring the body compositions, blood pressure and physical fitness before and after applying the program. Data were collected between April and June in 2006, and analyzed by χ2-test, t-test, ANCOVA using a SPSS 11.5 program. Results: There were statistically significant in percent of body fat, muscle strength, muscle endurance, flexibility, and balance ability, but no significant differences were found in body weight, systolic pressure and diastolic pressure. Conclusion: Exercise using Thera Band can be an effective nursing intervention to improve percent of body fat, muscle strength, muscle endurance, flexibility and balance ability in the elderly women.

중환자 전문간호행위에 대한 건강보험 상대가치 및 환산지수 개발

김진현(Kim, Jin Hyun),김명애(Kim, Myung Ae),김미원(Kim, Mi Won),김경숙(Kim, Kyung Sook),유정숙(Yoo, Cheong-Suk) 한국간호과학회 2011 Journal of Korean Academy of Nursing Vol.41 No.3

4배성 휘커스(Ficus-4x)오디품종의 영양성분 및 항산화능 검색

김애정(Ae-Jung Kim),김미원(Mi-Won Kim),우나리야(Na-ri-Yah Woo),김선여(Sun-Yeou Kim),김현복(Hyun-Bok Kim),임영희(Young-Hee Lim),김명희(Myung-Hee Kim) 한국식품과학회 2004 한국식품과학회지 Vol.36 No.6

충남 일부 지역 대학생의 식사의 질, 식행동 및 에너지 평형상태 조사 연구

김명희 ( Myung Hee Kim ),김무경 ( Moo Kyung Kim ),최미경 ( Mi Kyeong Choi ),김동희 ( Dong Hee Kim ),김미원 ( Mi Won Kim ) 한국식품영양학회 2012 韓國食品營養學會誌 Vol.25 No.3

중학생의 학교폭력과 예방교육에 대한 인식 연구

김미원(Kim, Mi Won),박옥임(Park, Ok Im),문희(Moon, Hee) 한국지역사회생활과학회 2014 한국지역사회생활과학회 학술대회 자료집 Vol.2014 No.10

전문 간호사 제도 현황 및 발전 과제

김미원 ( Mi Won Kim ) 연세대학교 간호정책연구소 2006 간호학탐구 Vol.15 No.2

본 글은 90년대 초부터 정책을 추진한 결과 2000년도에 이르러 법제화되기 시작하여 2006년 첫 시험을 통해 전문간호사를 배출하기까지 전문간호사 제도화의 경과를 정리하고 현황을 확인 한 후 미래 방향을 짚어 보고자 하는 목적으로 작성되었다. 이와 같이 전문간호사 제도의 중요한 사항들에 대한 그동안 정책적 추진 경과를 검토하여 보는 것은 현재 전문간호사의 정착과 발전에 요구되는 많은 과제에 대한 해결방향을 찾는데 도움이 될 것으로 기대한다. 구체적 내용으로는 전문간호사 관련 법령의 변화, 전문간호사 분야의 변화, 전문간호사의 자격, 전문간호사 역할 및 직무, 전문간호사 교육, 전문간호사 시험에 대해 정리하였다. 논의에서는 이와 같은 경과를 정리함에 따라 확인된 문제와 앞으로 정착하고 발전해 나가기 위한 추후 발전과제와 방향을 짚어 보았다. Since a policy of Advanced Practice Nursing was proceeded from the beginning of the 1990s, it started to be legally institutionalized in 2000 and advanced practice nurses were finally produced through its first examination in 2006. This study aimed to arrange the development of institutionalization of Advanced Practice Nursing so far and conform its present condition so as to examine its future course. It`s expected that reviewing on the development in policy so far for important factors of Advanced Practice Nursing would be of help to find a solution to many assignments needed for the settlement and development of current Advanced Practice Nursing. This study specifically arranged a change of relative laws of Advanced Practice Nursing, change of the relative field, qualifications for Advanced Practice Nurse, roles and duties of the job, Advanced Practice Nurse education and examinations. From the discussion, this study arranged this development so as to examine the confirmed matter, further assignments for development and courses to be settled and developed in the future.

팀구성 방법에 따른 학습효과

김미원(Kim, Mi-Won),이희주(Lee, Hee-Joo) 한국웰니스학회 2016 한국웰니스학회지 Vol.11 No.4

본 연구는 학습효과를 높이기 위한 팀 구성 방법을 확인하고자 시행하였다. 본 연구는 1개 대학 간호학과 3학년 54명을 대상으로, 팀구성 방법(성격 외향성 요인, 무작위, 성적)에 따른 학습효과와 만족도를 파악하기 위해 이루어졌다. 동일대상에 대하여 3가지 팀구성 방법을 각 5주간 씩, 3차에 걸쳐 모두 적용하고 반복측정 한 유사실험연구이다. 자료는 SPSS 21.0 통계프로그램을 사용하였으며, 일반적 특성 등은 빈도와 백분율, 평균, 표준편차 적용, 팀구성 방법에 따른 학습효과 및 만족도는 반복측정 분산분석(Repeated Measured ANOVA)으로 분석하였고, 성격요인점수가 리더역할과 참여정도에 미치는 영향은 단순 회귀분석을 사용하였다. 연구결과 성격 외향성 요인을 기준으로 수준의 다양성을 가진 팀을 구성한 경우 문제해결(p=.000), 협동(p=.009), 통합(p=.000) 등의 학습효과와 참여만족, 활동만족, 결과만족도가 가장 높았다(p=.015, p=.008, p=.001). 또한 5개 성격요인 점수 중 외향성과 성실성이 개인의 팀활동에서의 리더역할(R2=.308, p=.000, R2=.228, p=.000)과 참여 정도((R2=.308, p=.000, R2=.228, p=.000)에 유의하게 영향을 미치는 것으로 나타났다. 이에 학습효과를 높이기 위한 팀구성 방법으로 외향성의 요인을 기준으로 한 성격관련 구성방법을 활용할 수 있는 근거를 제시하였다. This study was carried out to ascertain a method to organize teams to enhance learning effects. This study targeted 54 junior nursing students of a university. To identify learning effects and satisfaction according to team organizing method (outgoing personality factors, random, and grade), this study conducted a quasi-experiment, which this study applied three team organizing methods to the same subjects three times for five weeks, respectively, and repeatedly measured. The SPSS 21.0 statistical program was used for data processing, and general characteristics were analyzed by frequency, percentage, mean, and standard deviation, and learning effects and satisfaction according to team organizing methods were analyzed with repeated measured ANOVA. For the influence of personality factor scores on leader’s role and participation level, a simple regression analysis was used in this study. As a result of this study, such learning effects as problem solving (p=.000), cooperation (p=.009), and integration (p=.000), and satisfaction with participation, satisfaction with activity, and satisfaction with result were the highest in order (p=.015, p=.008, p=.001, respectively) in the case of organizing teams, based on the extroversion factor. Among the five personality factors, extroversion and sincerity significantly affected personal leader role (R2=.308, p=.000, R2=.228, p=.000) and participation level ((R2=.308, p=.000, R2=.228, p=.000). Therefore this study presented a basis to utilize the personality-related organizing method, based on the extroversion factor, as the method to organize teams in order to enhance learning effects.