니아울리 잎 추출물의 항산화 효과 및 성분 분석

김문진,김은종,박수남,Kim, Moon Jin,Kim, Eun Jong,Park, Soo Nam 한국응용과학기술학회 2014 한국응용과학기술학회지 Vol.31 No.4

니아울리 잎 추출물의 항산화 효능과 성분 분석에 관한 연구를 시행하였다. 실험에 사용한 시료는 건조된 니아울리 잎의 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획을 이용하였다. 자유라디칼(1,1-phenyl-2-picrylhydrazyl, DPPH) 소거활성($FSC_{50}$)은 에틸아세테이트 분획이 $10.05{\mu}g/mL$로, 지용성 항산화제로 알려진 (+)-${\alpha}$-tocopherol ($8.89{\mu}g/mL$)과 유사한 효과를 나타내었다. 루미놀 발광법을 이용한 $Fe^{3+}-EDTA/H_2O_2$ 계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 니아울리 잎 추출물의 총 항산화능($OSC_{50}$)에서 에틸아세테이트 분획은 $1.61{\mu}g/mL$, 아글리콘 분획은 $1.07{\mu}g/mL$를 나타냈다. 에틸아세테이트 분획과 아글리콘 분획 모두 항산화 활성이 매우 큰 비교 물질인 L-ascorbic acid ($1.50{\mu}g/mL$)와 비슷한 활성산소 소거활성을 나타내었다. Rose-bengal로 광증감된 $^1O_2$에 의한 세포손상에 대한 세포보호능 측정에서 추출물 및 분획 모두 농도 의존적($5{\sim}50{\mu}g/mL$)으로 세포보호 활성을 나타내었다. 특히 아글리콘 분획과 50% 에탄올 추출물의 ${\tau}_{50}$은 $10{\mu}g/mL$에서 각각 158.80 min, 50.1 min으로 지용성 항산화제로 알려진 (+)-${\alpha}$-tocopherol (38.0 min)보다 더 큰 세포 보호 효과를 나타내었다. TLC와 HPLC를 이용하여 니아울리 잎 추출물의 에틸아세테이트 분획으로부터 avicularin과 quercitrin이 존재함을 처음 확인하였다. 이상의 결과들은 니아울리 잎 추출물 또는 분획이 항산화 원료로서 화장품에 응용 가능함을 시사한다. The antioxidative effects and component analysis of the Melaleuca quinquenervia leaf extracts were investigated. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from dried M. quinquenervia leaves. The DPPH (1,1-phenyl-2-picrylhydrazyl) scavenging activity ($FSC_{50}$) of ethyl acetate fraction ($10.05{\mu}g/mL$) of M. quinquenervia leaf extracts was similar to (+)-${\alpha}$-tocopherol($8.89{\mu}g/mL$) known as a typical antioxidant. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of the ethyl acetate fraction ($1.61{\mu}g/mL$) and aglycone fraction ($1.07{\mu}g/mL$) of leaf extracts of M. quinquenervia on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system using the luminol-dependent chemiluminescence assay were similar to that of L-ascorbic acid ($1.50{\mu}g/mL$). The cellular protective effect of the extracts on the rose bengal sensitized photohemolysis of human erythrocytes was increased in a concentration dependant manner ($1{\sim}50{\mu}g/mL$). Especially, the cellular protective effects of Aglycone fraction (${\tau}_{50}=158.80min$) and 50% Ethanol extract (${\tau}_{50}=50.1{\pm}0.2min$) on the $^1O_2$-induced cellular damage of human cells were exhibited the higher than (+)-${\alpha}$-tocopherol (${\tau}_{50}=38.0min$). TLC and HPLC were used to analyse active components in the ethylacetate fraction of the extracts. Results showed that avicularin and quercetrin were active components of the extracts. These findings suggest that the M. quinquenervia leaf extracts can be applied to new cosmetics products as an effective antioxidant ingradient.

한국 상괭이(Neophocaena asiaeorientalis)의 구조, 재활, 방류

김문진(Moon-Jin KIM),손호선(Hawsun SOHN) 한국수산해양교육학회 2016 水産海洋敎育硏究 Vol.28 No.3

The coastal water of Korean Peninsula is the major habitat for finless porpoises. However, hundreds of finless porpoises are currently caught incidentally by stow-net fishery every year. To prevent the mass mortality of finless porpoises and conserve marine animals, Busan Aquarium has been designated as a “Marine Life Ex-Situ Conservation Institution and Marine Animal Conservation Center,” and Cetacean Research Institute, under the National Institute of Fisheries Science have started RRR (rescue, rehabilitation and release) project together since 2011. From 2012 to 2013, four individuals of finless porpoises had been rescued and two of them finally released to their original habitat after fully recovered in July 2013. As a result, according to growth curve of finless porpoise of Korea more than 140 ㎝ of body length, 38 ㎏ of total weight referred to adult, results of observation of the change in the hormone revealed that mating time of finless porpoises is January, Jun and July. To become the initial institution which rescues, treatment, husbandry and releases finless porpoise in Korea.

염분변화가 대왕바리, Epinephelus lanceolatus의 산소소비에 미치는 영향

김문진(Moon-Jin KIM),임한규(Han-Kyu LIM),정민환(Min-Hwan JEONG) 한국수산해양교육학회 2015 水産海洋敎育硏究 Vol.27 No.2

Changes of oxygen consumption in juvenile giant grouper, Epinephelus lanceolatus were investigated in order to find out the physiological responses associated with freshwater and low salinities acclimation. Salinity changes in this experiment were set as follows; 35 psu (Exp. Ⅰ) as control, decrease in the manners of 35→20→10→0 psu (Exp. Ⅱ), 35→10→0 psu (Exp. Ⅲ) and 35→0 psu (Exp. Ⅳ). The average oxygen consumption at 27℃ in Exp. Ⅰ (control) was 106.9±0.7 mg O₂/kg/h. In Exp. Ⅱ, according to the changes of 35→20→10→0 psu in freshwater acclimation, fish showed the gradual decrease in oxygen consumption as 108.1±2.1, 99.6±2.9, 74.6±0.9 and 62.7±1.0 mg O₂/kg/h, respectively. The fish in Exp. Ⅲ also revealed the same tendency as 106.5±1.3, 77.0±1.2 and 64.7±1.2 mg O₂/kg/h, respectively. In Exp. Ⅳ, fish showed the decreased oxygen consumption from 109.0±0.9 mg O₂/kg/h of 35 psu to 71.6±2.0 mg O₂/kg/h of 0 psu. Giant grouper is thought to be a diurnal fish species, because the level of oxygen consumption during light period was higher than that during dark period.

극소 저출생 체중아에서 SMOFlipid의 투여가 미숙아 질환 예방에 미치는 영향: Cholestasis 예방 중심으로

김문진 ( Moon Jin Kim ),배혜정 ( Hye Jung Bae ),조윤숙 ( Yoon Sook Cho ),김귀숙 ( Kwi Suk Kim ),김향숙 ( Hyang Suk Kim ),이혜숙 ( Hye Suk Lee ),이주영 ( Ju Young Lee ),김이경 ( Ee Kyung Kim ),김한석 ( Han Suk Kim ) 한국정맥경장영양학회 2013 한국정맥경장영양학회지 Vol.5 No.2

Purpose: Very low birth weight infants (VLBWI) are found to have a higher incidence of cholestasis due to long-term total parenteral nutrition (TPN). Although there have been some studies on relationships between omega-3 fatty acids and reduced incidence of cholestasis, the advantages that the fatty acids provide for premature infant diseases have not yet been fully understood. The aim of this study was to evaluate the advantages of SMOFlipid, a lipid emulsion product that contains omega-3 over other lipid emulsion products that do not. Methods: The medical records of 182 newborn (127 boys and 55 girls) were reviewed retrospectively between April 2010 and August 2012. These infants were born with a birth weight of <1,500 g and administered either of lipid emulsions (Intra MCT 20% or SMOFlipid) from birth to a full-feeding condition at our neonatal intensive care unit. Cholestasis is defined as serum direct bilirubin (DB) >1.0 mg/dL when total bilirubin (TB) is <5 mg/dL or DB/TB ratio is >20% when TB >5 mg/dL. Results: The incidence of cholestasis was found to be lower in the SMOFlipid group than in the medium-chain triglyceride/long chain triglyceride (MCT/LCT) group (7.3% [4/55] versus 18.9% [24/127], P=0.046). At the start of lipid emulsion administration (day 0), the baseline bilirubin level showed no significant difference between the two groups. However, the maximum value of DB on days 7∼ 14 was lower in the SMOFlipid group than in the MCT/ LCT group (0.8 [0.3∼2.2] versus 1.1 [0.3∼2.8] mg/dL, P= 0.030). The DB/TB ratio was also lower in the SMOFlipid group than in the MCT/LCT group (10.2% [4.9%∼40.0%] versus 24.1% [5.1%∼62.5%], P=0.002). Conclusion: This study concluded that SMOFlipid was effective in the prevention of cholestasis in VLBWI. Therefore, omega-3-containing lipid emulsion can be highly recommended in premature babies, especially in VLBWI who require TPN support. (JKSPEN 2013;5(2):67-75)

Visipaque(R) 조영제 사용 후 발생한 후기 부작용

김차영 ( Cha Young Kim ),김희진 ( Hee Jin Kim ),김문진 ( Moon Jin Kim ),강여진 ( Yeo Jin Kang ),김현정 ( Hyun Jung Kim ),장세호 ( Se Ho Chang ),박동준 ( Dong Jun Park ) 대한내과학회 2011 대한내과학회지 Vol.81 No.1

Late adverse reactions to iodinated contrast medium are defined as reactions occurring 1 hour to 1 week after contrast medium injection. The majority of late adverse reactions are cutaneous reactions, and include itching, maculopapular rash, urticaria, erythematous patches, and angioedema. We report a patient with an iodixanol (Visipaque(R))-induced late adverse reaction. She was admitted for evaluation of nephrotic range proteinuria and edema. Abdomen and chest enhanced computed tomography was performed as a malignancy work-up. Sixteen hours after radiocontrast exposure, her entire body felt itchy. A maculopapular rash was first detected on her back and abdomen 24 hours after exposure, and spread over her entire body, including her face. Her skin lesions resolved completely in 5 days after administering steroid and antihistamine. We should consider late adverse reactions of iodinated contrast medium when we detect skin lesions late after radiocontrast use. (Korean J Med 2011;81:130-134)

쿼세틴과 루틴을 담지한 양이온 리포좀의 특성조사 및 UVA에 대한 세포 보호 효과

구현아 ( Hyun A Gu ),김문진 ( Moon Jin Kim ),김해수 ( Hae Soo Kim ),하지훈 ( Ji Hoon Ha ),유은령 ( Eun Ryung Yu ),박수남 ( Soo Nam Park ) 한국공업화학회 2015 공업화학 Vol.26 No.2

Quercetin and its glycoside, rutin, are flavonoids, which are well known as natural antioxidants. In this study, cationic liposomes loaded with flavonoids (quercetin or rutin) were investigated for their effects on cell and skin permeability, and protective effects against UVA. The particle size of the empty cationic liposomes was in the range of 100∼130 nm, and the zeta potential was + 33.05 mV. The entrapment efficiency of 0.5R/CL was higher than that of 0.5 Q/CL. The cellular uptake of the cationic liposomes was five-fold higher than that of liposomes. The skin permeability of quercetin and rutin was investigated using Franz diffusion cells. Compared to the initial loading dose, the amount of quercetin or rutin delivered to the skin by cationic liposomes was higher than that delivered by conventional liposomes or phosphate-buffered saline. From the protective effect of cationic liposomes against UVA (25 J/cm2), we found that the cell viability in cationic liposomes containing flavonoids was higher than that of using UVA irradiation only. These results indicate that cationic liposomes provide enhanced delivery of flavonoids (quercetin and rutin) into the skin and may be used for antiaging and antioxidant cosmetics.

수온 및 광조건에 따른 대왕바리 Epinephelus lanceolatus의 산소소비 특성

정민환 ( Min Hwan Jeong ),김상덕 ( Sang Duck Kim ),임한규 ( Han Kyu Lim ),김문진 ( Moon Jin Kim ),김봉석 ( Bong Seok Kim ) 한국어류학회 2014 韓國魚類學會誌 Vol.26 No.4

본 연구에서는 수온 및 광조건에 따른 대왕바리 Epinephelus lanceolatus의 산소소비 특성을 조사하였다. 수온 17, 22,27 및 32.C에서 대왕바리의 산소소비량은 각각 61.7±0.4,72.2±0.6, 102.9±0.8 및 141.7±1.0 mg O2/kg/h으로 수온상승과 비례하여 증가하였다. 광조건은 명기 (06:00~18:00h)와 암기(18:00~06:00 h)는 각각 12시간씩 주었으며, 수온 17.C에서 명기와 암기의 산소소비량은 각각 62.7±0.4,62.5±0.3 mg O2/kg/h로 유의한 차이를 보이지 않았다(P¤0.05). 수온 22.C에서 명기와 암기의 산소소비량은 각각74.8±0.7, 69.6±0.6 mg O2/kg/h, 수온 27.C에서는 107±1.2, 98.0±0.7 mg O2/kg/h, 그리고 수온 32.C에서는 147.6±1.1, 135.8±0.8 mg O2/kg/h로 암기보다 명기에 산소소비가 많은 경향을 보였다. This study investigated the effe℃ts of water temperature and photoperiod on oxygen ℃onsumption (O℃) in the giant grouper Epinephelus lan℃eolatus. O℃ rate in the giant grouper at 17, 22, 27 and 32℃ were 61.7±0.4, 72.2±0.6, 102.9±0.8 and 141.7±1.0 mg O2/kg/h, respe℃tively, indi℃ating a linear in℃rease in O℃ with water temperature. Photoperiod was regulated in a℃℃ordan℃e with the light (06:00~18:00 h, L) and dark (18:00~06:00 h, D) phases of the diel ℃y℃le (12L/12D), with a water temperature of at 17, 22, 27 and 32℃. O℃ rates during the light and dark phases were 62.7±0.4, 62.5 ±0.3 mg O2/kg/h, respe℃tively, at 17℃. No signifi℃ant differen℃es were observed between the light and dark phases (P¤0.05). O℃ raters during the light and dark phases were 74.8±0.7, 69.6±0.6 mg O2/kg/h at 22℃, 107±1.2, 98.0±0.7 mg O2/kg/h at 27℃ and 147.6±1.1, 135.8±0.8 mg O2/kg/h at 32℃, respe℃tively, indi℃ating that O℃ is higher during daylight than nighttime. Giant grouper is thought to be a diurnal fish spe℃ies, be℃ause the level of oxygen ℃onsumption during light period was higher than that during dark period.

두충 추출물을 담지한 에토좀의 특성 및 경피 전달

구현아 ( Hyun A Gu ),김해수 ( Hae Soo Kim ),김문진 ( Moon Jin Kim ),유은령 ( Eun Ryeong Yu ),조기정 ( Gi Jung Joe ),장진동 ( Jin Dong Jang ),김보현 ( Bo Hyeon Kim ),박수남 ( Soo Nam Park ) 한국공업화학회 2013 공업화학 Vol.24 No.6

In this study, ethosomes loaded with Eucommia ulmoides extracts that have anti-inflammatory and anti-aging activities on the skin were prepared as cosmetic materials. The particle size, physical property, and entrapment efficiency of ethosomes to identify their stabilities during 4 weeks were measured. Ethosomes containing 0.01∼0.05% E. ulmoides extracts did not show either precipitation or separation. The mean particle size of these ethosomes was 136.4 nm, and monodisperse. The particle size of ethosome containing 0.05% E. ulmoides extracts was 151.15 nm and the entrapment efficiency was 81.79%. Therefore, in vitro skin permeation experiment was performed with ethosome containing 0.05% E. ulmoides extracts that showed the high loading efficiency and stability during 4 weeks. The order of skin permeation of E. ulmoides extracts was ethosome (77.16%) > liposome (62.80%) > ethanolic solutoin (41.59%). These results suggest that ethosomes containing 0.05% E. ulmoides extracts are effective for the skin permeation and may be used as a cosmetic formulation containing plant extracts.

원산지별 감초추출물의 항산화활성 증가를 위한 효율적인 추출조건 탐색

하지훈 ( Ji Hoon Ha ),이혜미 ( Hye Mi Lee ),권순식 ( Soon Sik Kwon ),김해수 ( Hae Soo Kim ),김문진 ( Moon Jin Kim ),전소하 ( So Ha Jeon ),정유민 ( Yoo Min Jeong ),황준필 ( Jun Pil Hwang ),박종호 ( Jong-ho Park ),최영기 ( Yung-key 대한화장품학회 2013 대한화장품학회지 Vol.39 No.4

본 연구에서는 한국 및 중국산 감초(Glycyrrhiza uralensis), 그리고 우즈베키스탄산 감초(Glycyrrhiza glabra)를 대상으로 추출용매, 추출온도, 추출시간 등 추출조건별 추출물을 제조하고 이들 추출물들의 추출 수율과 항산화 활성을 비교하여 최적의 추출조건을 선정하였다. 항산화 활성 중 자유 라디칼(1,1-phenyl-2-pic-rylhydrazyl, DPPH) 소거활성은 85% 에탄올로 60 ℃에서 6 h 동안 추출한 조건에서 한국 감초로부터 얻은 추출물이 가장 높은 활성(46.05%)을 나타내었다. 루미놀 발광법을 이용한 추출물들의 총 항산화능 측정 실험과 피부 광노화에 중요한 ¹O₂으로 유도된 세포막 손상에 대한 추출물들의 세포 보호 효과를 측정한 실험 모두에서도 위의 조건에서 가장 높은 항산화 활성을 나타내었다. 특히, 한국 감초는 τ₅₀이 116.4 min으로 비교 물질인(+)-α-tocopherol (28.5 min)보다 약 4 배나 높은 세포 보호 효과를 나타내었으며, 추출 수율은 18.75%로 우즈베키스탄 및 중국 감초보다 각각 1.2 배 및 2.5 배의 추출 수율을 나타내었다. 따라서, 본 연구 결과는 항산화 소재로 화장품에 응용하기 위하여 감초로부터 추출물을 얻기 위한 최적의 조건은 85% 에탄올로 60 ℃에서 6시간 동안 추출하는 것임을 보여주었다. In this work, licorice extracts were prepared using various extraction conditions such as extraction solvent, temperature, and time from Glycyrrhiza uralensis (G. uralensis) produced in Korea and China and Glycyrrhiza glabra (G. glabra) in Uzbekistan. The optimum extraction condition was selected from the extraction yields and antioxidative activities of extracts. Korea licorice extracts showed the highest free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scav-enging activity (46.05%) under the extraction condition of 85% ethanol at 60 ℃ for 6 hours. The prominent ROS (reactive oxygen species) scavenging activity using luminol-dependent chemiluminescence assay and the cellular protective effect against ¹O₂ induced cellular membrane damage were also shown from the extracts obtained from the same condition. Especially, Korea G. uralensis extracts exhibited the higher prominent protective effect (τ₅₀ = 116.4 min) than (+)-α-tocopherol (τ₅₀ = 28.5 min) and the extraction yield of Korea licorice extract was 18.75%, which is 1.2 times and 2.5 times higher than that of Uzbekistan and China, respectively. These results indicate that the extraction condition of 85% ethanol at 60 ℃ for 6 hours is optimal to prepare licorice extracts, which can be applicable as antioxidative cosmetic materials.

SCM415강의 침탄 표면처리 공정과 기계적 성질

김대욱,김문진,임병수 成均館大學校 科學技術硏究所 1998 論文集 Vol.49 No.2

The influence of plasma carburizing process on the surface hardness of SCM415 low-alloy steel (0.15% C) was investigated under the various process conditions of gas composition, gas pressure, plasma current density, temperature and time. The effective case depth was found to depend on the amount of methan gas containing carbon, thus the deepest case depth and the uniformity of hardeness were obtained under the 100% methan gas condition. The case depth increased with the higher plasma current density which could be increased in the higher gas pressure, because the surface carbon content depends on plasma current density. The effective plasma carburizing temperature of SCM415 steel was found to be higher than 850℃, and the case depth was proportional to the square root of carburizing time under the same current density. The bending fatigue strength of plasma carburized specimen is higher than those of as-received specimen and reheat-quenched specimen. High cycle fatigue and dry wear characteristics of vacuum carburized low alloy steel, SCM415(0.15% C), were evaluated after various heat treatments including direct quenching, single reheat quenching and sub-zero treatment. Comparing the fatigue and wear characteristics of carburized specimens to those of hot annealed or reheat quenched specimens, the wear resistant property as well as bending fatigue limits of vacuum carburized steels were substantially superior to other heat treated steels. Test results indicated that the carburized and reheat treated specimens have higher fatigue limits than the carburized and direct-quenched specimens and the specimens followed by sub-zero treatment. The wear volumes of sub-zero treated specimens with higher surface hardness show slightly lower values than those of non sub-zero treated specimens. This is attributed to the reduction of retained austenite amount of the surface and an increase of surface hardness by the sub-zero treatment effect.