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형질전환 벼 현탁세포 배양에서 세포 사멸 억제를 통한 hCTLA4Ig 생산성 증대
김명식(Myong-Sik Kim),남형진(Hyung-Jin Nam),김민섭(Min-Sub Kim),권준영(Jun-young Kwon),김동일(Dong-Il Kim) 한국생물공학회 2013 KSBB Journal Vol.28 No.4
Transgenic plant cell cultures are an attractive expression system for the production of industrial and pharmaceutical proteins because of their advantages in safety and low production cost. Human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) was produced and secreted when sugar was depleted in culture medium by transgenic rice cell lines (Oryza sativa L.) using RAmy3D promoter. Due to the production of the target protein by sugar depletion, concomitant occurrence of cell death is inevitable. For that reason, inhibition of cell death for enhancing productivity was necessary for the production period without energy sources. Supplementation of 0.1 mM sodium nitroprusside improved cell viability by 1.4-fold and maximum hCTLA4Ig production by 1.3-fold compared to those of control. Addition of 1 and 10 mM glutathione, N-acetylcysteine (NAC), and nicotinamide inhibited apoptotic-like programmed cell death by decreasing the activity of reactive oxygen species. Production hCTLA4Ig was enhanced 1.4-, 1.25-, and 1.15-fold with 10 mM NAC, 1 mM NAC, and 1 mM glutathione, respectively. In addition, it was found that the supplementation of NAC enhanced the cell viability.
알코올성 췌장염과 간경변 환자에서 알코올대사효소와 시토카인의 유전자 다형성
김명식 ( Myong Sik Kim ),이돈행 ( Don Haeng Lee ),강효승 ( Hyo Seung Kang ),박현신 ( Hyun Shin Park ),정석 ( Seok Jung ),이진우 ( Jin Woo Lee ),권계숙 ( Kye Sook Kwon ),김범수 ( Pum Soo Kim ),김형길 ( Hyung Gil Kim ),신용운 ( Yon 대한소화기학회 2004 대한소화기학회지 Vol.43 No.6
Background/Aims: Susceptibility to organ damage induced by alcohol may be related to inherited variations (polymorphisms) in alcohol-metabolizing enzymes, or polymorphisms affecting cytokines. The aim of this study was to compare the genotype and allelic frequencies of ADH2, ADH3, ALDH2, cytochrome P450-2E1, IL-1, IL-6, IL-8 and tumor necrosis factor-α in patients with alcoholic pancreatitis and alcoholic liver cirrhosis with those of controls. Methods: We determined the polymorphism of genes of the above-mentioned alcohol-metabolizing enzymes and cytokines in 29 alcoholic pancreatitis patients (AP), 22 alcoholic liver cirrhosis patients (LC) and 100 healthy blood donors (control). The genotypes were characterized by restriction fragment length polymorphism after amplification of genomic DNA by polymerase chain reaction. Results: The allelic frequency of CYP2E1*c2 was significantly different in three groups (AP: LC: Control=0.224: 0.136: 0.320, p<0.05). There was no significant difference in the other genotypes or allelic frequencies of the three groups. The allelic frequencies of CYP2E1*c2 and ALDH2*2 were more frequent in the control than patients with alcoholic liver cirrhosis (LC: Control=0.136: 0.320, p<0.05, LC: Control=0.114: 0.265, p<0.05). Allelic frequencies of ADH2 was statisitcally different between LC and control (ADH2*1; LC: Control=0.727: 0.495, ADH2*2; 0.227: 0.360, ADH2*3; 0.046: 0.145, p<0.05). Conclusions: There was no difference in the frequencies of genotype and allele of enzymes and cytokines among the three groups. However, frequency of ADH2*1 was significantly higher and those of CYP2E1*c2 and ALDH2*2 were significantly lower than LC group than control. (Korean J Gastro-enterol 2004;43:355-363)
성인에서 반복적인 복부통증을 유발한 Meckel 게실염에 의한 복막유착
김영수(Young Soo Kim),신용운(Yong Woon shin),문희용(Hee Yong Moon),김명식(Myong Sik Kim),권계숙(Kye Sook Kwon),방제소(Jei So Bang),조현근(Hyeon Geun Cho),김범수(Pum Soo Kim),김준미(Joon Mee Kim) 대한소화기학회 1997 대한소화기학회지 Vol.30 No.6
Meckel,s diverticulum is generally acknowledged to be the most prevalent congenital anomaly of the gastrointestinal tract. In the autopsy series, the incidence ranged from 0.5 to 2 percent. The preoperative diagnosis of Meckels divemiculum is difficult, especially in the adult, because of the lesions ability to masquerade as one of a variety of much more common abdominal pathologies. The diagnostic accuracy may be affected by the age of the patient and by complications of diverticulum. The complications that may result include ulcer bleeding, intestinal obstruction, perforation, diverticulitis, stone, and neoplasm. The ways a Meckels diverticulum can cause bowel obstruction are different. Recently we experienced a case of Meckels diverticulitis causing intlammatory reaction and adhesion with the abdominal wall and causing recurrent abdominal pain and obstruction in a 22-year-old woman. Therefore, we present this case with a brief review of the related literature. (Korean J Gastroenterol 1997; 30:826-830)
일본 장애인 소규모 공동작업장의 실태분석과 한국에의 시사점
이채식,조원일,이은영,김명식 한국장애인고용촉진공단 2008 장애와 고용 Vol.18 No.2
본 연구는 일본에서 장애인 당사자의 입장을 직업기회와 사회참여 활동의 주요한 장소제공과 고용창출을 도모하는 소규모 공동작업장의 실태조사를 통해 현재 우리나라 중증장애인고용창출을 위한 발전방향에 대해 시사점을 제시하는데 그 목적을 두고 있다. 이러한 연구목적을 위해 일본 장애인 소규모 공동작업장 147개소를 대상으로 설문조사를 실시하여 회수된 총 81부에 대해 일반사항, 경영사항, 장애인고용현황, 작업장 만족현황에 대해 통계분석을 한 결과, 장애의 강점을 발굴하여 실제 직업현장과 연계, 직업생활과 사회생활의 연계, 적극적인 생산성 확대 모색, 작업장의 민주적 운영, 지역사회 내 여가활동의 장 마련, 작업장 운영의 운동성과 유연성을 기반으로 하는 특징이 나타났다. 이러한 일본 장애인 소규모 공동작업장의 운영실태가 우리나라 중증장애인의 고용기회 확대와 당사자 중심의 고용환경을 조성하는데 주요한 시사점을 제시하고 있다. The purpose of this study provide a basic data on employment development for the severe disabled in Korea by the small scale common workshop in Japan which the employment opportunity and social participation for the disabled. For this purpose, the small scale common workshop 147 in japan surveyed meaning subject 91, general facts, management facts, the disabled employment situation, workshop satisfaction situation. To this end, the small scale common workshop in Japan have these characteristics, finding the disability empowerment and then link with job situation, the vocational life link with social life, the active productivity expansion, provide a leisure setting in community, the movement and flexibility of workshop. The managements of small scale common workshop in Japan suggest on employment opportunity development for the severe disabled and create for employment environment focused on the parties in Korea.
아미노산 수액의 신속한 정맥내 주사 후 담낭 수축에 관한 연구
김미영,김영수,최원,장재남,김명식,김인한,신용운,권계숙,이돈행,오윤주,김형길,이재수,김범수,조현근 대한소화기학회 1999 대한소화기학회지 Vol.33 No.1
Background/Aims: Gallbladder (GB) sludges and/or gallstones frequently develop due to hypomotility of GB after long-term total parenteral nutrition, abdominal surgery, bone marrow transplantatation, AIDS infection, trauma. The purpose of this study is to promote GB contraction and emptying with the rapid intravenous infusion of aminoacids and then, to determine the most effective dosage and infusion rate. Methods: After infusion of aminoacids, the volume of GB was measured serially using ultrasonography before infusion and immediately (0 min), 15 min, 30 min, 45 min and 60 min after infusion. The subjects of this study were 28 healthy male volunteers aged from 23 to 26. For control group (n=4), 250 cc of normal saline was infused for 30 min. For group A (n=8), 250 cc of aminoacids solution was infused for 30 min (21.2 g, 0.7 g/min). For group B (n=8), 250 cc of aminoacids solution was infused for 10 min (21.2 g, 2.1 g/mn). For group C (n=8), 125 cc of aminoacids solution was infused for 5 min (10.6 g, 2.1 g/min). Results: The volume of GB was significantly decreased with the lapse of time in A, B, C groups and the most significant change occurred at 45 min after infusion (p=0.0001). These groups showed significant volume change com pared to control group (p=0.0029). At 15 min after infusion, significant GB contraction occurred in group B, C compared to control group (p=0.0030). Only B group showed significant GB contraction at 45 min after infusion (p=0.0041). Conclusions: It is concluded that the intermittent rapid intravenous minoacids infusion may be useful to prevent GB sludges in the high risk groups. (Kor J Gastroenterol 1999;33:90 - 96)