A Study on Increasing Volunteers’ and Managers’ Satisfaction with Urban Park Management - Focus on the ‘Managing My Park in My Neighborhood’ program -

김대혁,최재혁,이시영 한국전통조경학회 2017 한국전통조경학회지 Vol.15 No.-

This study suggests measures to improve satisfaction of park managers and volunteers by conducting surveys on their awareness on the volunteering program called ‘Managing My Mark in My Neighborhood’ being carried out by the Daejeon Metropolitan City Government. The methods taken for this study are as follows: first, the study identified factors affecting the level of volunteers’ satisfaction and measures to effectively manage urban parks; then, the value and roles of volunteering for the management of urban parks were studied. Afterwards, volunteers who are currently participating in the program above were asked to participate in a survey, and park managers from the five district governments and the city government of Daejeon were interviewed in an attempt to seek for solutions to boosting the program. The results are as follows: First, the survey among the volunteers found that they joined the program to obtain volunteer hours, to maintain their relationships with some of the other volunteers, and just simply because they like parks. Second, the volunteers are satisfied with their participation because they can secure volunteer hours they need and feel they take the lead in managing the parks. Third, what the volunteers mostly do in the program is cleaning the parks. They address some challenges in facility management, implementation of park activities programs, and management of trees in the parks. Fourth, the volunteers feel that the most important challenges to boost the program would be to improve volunteers’ attitude, raise social awareness about volunteering, and reward volunteers. As for the result of the interview with park managers, most of them agreed on the necessity of continuing to promote volunteering for managing urban parks because they feel that they work more than their budget and human resources allow. Regarding the volunteering program, the interviewees shared a view that they need more diverse programs including managing trees other than just cleaning. Overall, the managers interviewed shared a less favorable view on the usefulness of the program due to their increased burden from work and responded that their workload needs to be lightened and that volunteers’ awareness should be raised further.

An auto weather-vaning system for a DP vessel that uses a nonlinear controller and a disturbance observer

김대혁,김낙완 대한조선학회 2014 International Journal of Naval Architecture and Oc Vol.6 No.1

An auto weather-vaning system for a Dynamic Positioning (DP) vessel is proposed. When a DP vessel is operating, its position keeping is hindered by ocean environmental disturbances which include the ocean current, wave and wind. Generally, most ocean vessels have a longitudinal length that is larger than the transverse width. The largest load acts on the DP vessel by ocean disturbances, when the disturbances are incoming in the transverse direction. Weather- vaning is the concept of making the heading angle of the DP vessel head toward (or sway from) the disturbance direc-tion. This enables the DP vessel to not only perform marine operations stably and safely, but also to maintain its posi-tion with minimum control forces (surge & sway components). To implement auto weather-vaning, a nonlinear control¬ler and a disturbance observer are used. The disturbance observer transforms a real plant to the nominal model without disturbance to enhance the control performance. And the nonlinear controller deals with the kinematic nonlinearity. The auto weather-vaning system is completed by adding a weather-vaning algorithm to disturbance based controller. Numerical simulations of a semi-submersible type vessel were performed for the validation. The results show that the proposed method enables a DP vessel to maintain its position with minimum control force.

심근 분획 혈류 예비력과 혈관내초음파를 이용한 관상동맥 협착정도의 비교

김대혁,권준,서정기,심성수,이효정,조성욱,박금수,이우형 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.8

Surface Displayed Expression of a Neutralizing Epitope of Spike Protein from a Korean Strain of Porcine Epidemic Diarrhea Virus

김대혁,김강주,조동하,김태금,양문식,박승문,Ae-Young Mo,Jung-Gu Lim,Hea-Jong Chung 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.6

The neutralizing epitope (K-COE) of the spike protein from a Korean strain of porcine epidemic diarrhea virus (PEDV) has been shown to prevent and foster an immune response to PED, when orally adjusted. The cell surface of the budding yeast, Saccharomyces cerevisiae, was engineered to anchor the K-COE on the outer layer of the cell, and consequently, the altered yeast was applied as a dietary complement for animal feed, with immunogenic functions. In this study, the K-COE gene (K-COE) of the Korean strain of PEDV with the signal peptide of rice amylase 1A (Ramy1A), was fused with the gene encoding the carboxyterminal half (320 amino acid residues from the C terminus) of yeast α-agglutinin, a mating associated protein that is anchored covalently to the cell wall. The glyceraldehyde-3-phosphate dehydrogenase (GPD) promoter was selected in order to direct the expression of the fusion construct, and the resulting recombinant plasmid was then introduced into S. cerevisiae. The surface display of K-COE was visualized via confocal microscopy using a polyclonal antibody against K-COE as the primary antibody, and FITC (fluorescein isothiocyanate)-conjugated goat anti-mouse IgG as the secondary antibody. The display of the K-COE on the cell surface was further verified via Western blot analysis using the cell wall fraction after the administration of α-1,3-glucanase/PNGase F/β-mannosidase treatment.

본문성을 고려한 설교본문 선정에 대한 연구

김대혁 한국복음주의실천신학회 2018 복음과 실천신학 Vol.46 No.-

To choose a preaching unit from a certain text and to confirm it as the foundation for the authority and relevance of preaching is the first task for the sermon-making process. In fact, choosing and confirm a preaching unit is not an easy but a complicated work that can influence the whole sermon-making process. Despite the importance of choosing a preaching unit for sermon preparation, there is a lack in hermeneutcal and homiletical studies or suggestions for it. In this respect, the main purpose of this article is to suggest a concrete suggestion for choosing a preaching unit―that is considering the textuality for a preaching unit. Accordingly, this paper first examines and evaluates comtemporary homileitcal theories(including expository preaching, new homiletics, and text-driven preaching scholars) regarding to choosing a preaching unit. Based upon the evaluations of them, this paper offers the necessity for choosing a preaching unit in consideration of textuality. Finally, this paper provides the efficiency and implications of choosing a preaching unit in consideration of textuality with an example of Mark 14:1-11. 설교가 지닌 권위와 적실성의 근거가 되는 성경 본문의 적정 단위를 선정하고 확정하는 일은 설교 작성을 위해 설교자가 가장 먼저 해야 할 과제이다. 하지만 설교본문 선정과 확정은 생각보다 어렵고 복잡한 일이며, 이는 설교 작성의 전체 과정에 영향을 미친다. 이처럼 설교본문 선정이 지닌 중요성이 큼에도 불구하고, 실제 설교자가 본문을 선정하고 확정하는 것에 대한 구체적인 해석학적/설교학적인 고민과 제안들이 부족한 것이 사실이다. 이러한 맥락 속에서 이 논문의 주요 목적은 설교본문 선정과 확정에 해석학적/설교학적 하나의 대안을 제언하는데 있다. 이를 위하여 우선 다수의 현대강해설교학자들, 신설교학자들, 본문의 중심성을 강조하는 최근의 설교학자들의 본문 선정에 관한 설명과 평가를 내린다. 이를 기준으로 설교단위 선정과 확정을 위한 구체적인 하나의 잣대로 본문성(textuality)을 고려한 설교본문 선정의 중요성과 필요성을 제시하고 있다. 이에 관한 증명으로 마가복음 14장 1절-11절의 본문의 단위를 살펴봄으로 본문성을 고려한 설교 본문 선정의 유효성과 그 함의들을 제공하고 있다.

본문성(Textuality)과 정경성(Canonicity)의 통합이 지니는 설교학적 함의

김대혁 한국복음주의실천신학회 2023 복음과 실천신학 Vol.68 No.-

To make accurate and effective biblical sermons, it is crucial to establish a connection between the biblical text and the modern audience. Although the terminology may vary, this process is generally understood as the harmonization of principilization and contextualization, which involves finding the intended meaning of the author and applying its relevance to the contemporary audience. This paper focuses on the theological process that includes principilization and contextualization to appropriately connect the past text with the modern audience, with particular emphasis on the harmony and integration of textuality and canonicity and their influence on the sermon-writing process. To accomplish this, the paper first examines the meaning of textuality and canonicity as integral aspects of God’s comprehensive communicative act, and then sequentially traces the developmental discussions of significant hermeneutical and homiletical models concerning the two facets of principilization and contextualization in the theological process. While acknowledging the necessity of principilization and contextualization based on the meaning-significance model in this theological process, it highlights the importance of integrating textuality and canonicity from the perspective of their unity as part of God’s communicative act. Finally, it presents the homiletical implications of the theological process with a focus on the integration of textuality and canonicity. 바르고 적실한 성경적 설교를 위해서는 성경 본문과 현대 청중을 연결하는 과정이 매우 중요하다. 비록 명칭은 다양하지만, 일반적으로 저자가 의도한 의미를 찾아 설명하며 오늘날 청중을 향한 의의를 적용하는 과정으로 원리화(principlization)와 상황화(contextualization)의 과정으로 이해된다. 이 논문은 과거 본문과 현대 청중 사이를 정당하게 연결하기 위한 원리화와 상황화를 포함하는 신학화 과정 가운데, 특별히 본문성(textuality)과 정경성(canonicity)의 조화와 통합의 중요성을 강조하면서그것이설교작성의과정에어떤영향을미치는지에집중한다. 이를 위해 이 논문은 우선 하나님의 총체적 소통행위로서 본문성과정경성의 의미를 살펴보고, 다음으로 신학화 과정에서 진행하는 원리화와상황화의두측면에대한주요해석학자들과설교학자들의발전적논의를 순차적으로 추적한다. 이 신학화 과정에서 의미-의의(meaningsignificance) 모델에 입각한 원리화와 상황화의 필요성은 인정하되, 이과정이 하나님의 소통행위로서 본문성과 정경성의 통합의 관점에서 이루어져야 할 것을 강조한다. 마지막으로 본문성과 정경성의 통합에 초점을 둔 신학화 과정이 지닌 설교학적 함의들을 제시한다

Cultural Characteristics and Extraction ofthe Fungal Pigment Phleichrome from thePhytopathogenic Fungus Cladosporium phlei

김대혁,박승문,김범태,양문식,황기준,Joong-Keun Lee,Jung-Ae Kim,Hea-Jong Chung 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.5

The cultural characteristics of the fungus Cladosporium phlei were assessed in order to develop an improved method for the production of the fungal pigment, phleichrome, which is an intermediate in the production of a photodynamic therapeutic agent. The growth of C. phlei, as measured by the hyphal growth rate and increase in biomass, varies significantly depending on the culture media utilized (V8 juice-based medium proved optimal for both growth rate and biomass increase). However, even on a V8 juice plate, the growth of C. phlei occurred slowly and in a limited fashion, in that the colony covered only 75% of the agar surface after more than 4 weeks of cultivation at 20℃. Supplementations of glucose, fructose, galactose, and sucrose increased both hyphal expansion and mass production, whereas supplementations of other carbon sources, including glycerol and sorbitol, exerted no detectable effects. The effect of inorganic nitrogen supplementation was negligible, whereas organic nitrogen evidenced significant effects, with enhanced growth with malt extract and growth inhibition with yeast extract and tryptone. Sporulation was enhanced under conditions of continuous light, and a minimum of 103 spores per mL of liquid media was found to be necessary for the optimal mass increase. A simple extraction procedure was established in order to isolate the deep red pigment which was subsequently identified as phleichrome via NMR analysis. When C. phlei was cultured on V8 medium containing 5% glucose and 2% malt extract, the quantity of mycelial mass was estimated as 20.6 g (dry weight) per liter of culture. The expected phleichrome yields from the mycelia and culture filtrates were estimated to be 43 and 2 mg/L, respectively.

성경에 나타난 ‘전유를 통한 적용’에 관한 해석학적∙설교학적 연구

김대혁 한국복음주의실천신학회 2020 복음과 실천신학 Vol.54 No.-

본 연구의 핵심 목적은 기존의 성경적 설교의 적용을 위한 원리화 과정에서 보편적 명제를 추출하는 방식이 지닌 약점에 대한 보완책으 로써, 전유를 통한 적용의 방식에 대한 성경적 근거를 확인하여 해석 학·설교학적 타당성을 확립하는데 있다. 이를 위해서 이 연구는 우선 ‘전유를 통한 적용’에 관한 개념을 간략하게 설명한다. 이를 토대로 신 약의 성경 저자들이 인용하거나 인유한 구약의 본문을 어떤 방식으로 해석하고 적용했는지를‘전유를 통한 적용’의 관점에서 살펴보고자 한 다. 특별히 이 연구는 사도행전 2장 14-36절, 고린도전서 10장 1-13 절, 히브리서 3장 7절-4장 14절의 본문을 각각 베드로, 바울, 히브리 서 저자의 설교로 이해하고, 각 본문을‘전유를 통한 적용’의 관점에서 자세히 연구한다. 끝으로 이러한 성경에 나타난 전유를 통한 적용의 사 례들이 가져다주는 해석학적·설교학적 함의들을 제시한다. The primary purpose of this study is to establish the hermeneutical and homiletical validity of ‘appropriated application’ with the biblical examples, which can be a supplementary model for overcoming the deficiencies of the existing principalizing model for sermon application. To do this, this study first provides a brief notion about ‘appropriated application.’ With the perspectives from ‘appropriated application,’ this study examines how the N.T. authors have interpreted and applied the O.T. texts, which they have quoted or alluded. In detail, this study deals with Acts 2:14-36, 1 Corinthians 10:1-13, and Hebrews 3:7-4:14, considering them as the ‘sermonic’ texts and providing their uses of ‘appropriated applications.’ Finally, with these biblical examples of ‘appropirated application,’ this study offers hermeneutical and homiletical implications towards today’s preachers.

‘본문에 충실한’ 설교를 위한 성경적 전유를 통한 적용에 관한 제안

김대혁 한국복음주의실천신학회 2019 복음과 실천신학 Vol.52 No.-

For biblical preaching that pursuits the life-change of the congregation, the preacher should rightly understand the meaning of the text and apply it legitimately to the contemporary audience. Thus, text-faithful application is a recurring task and responsibility for the preacher. Despite the consensus for the text-faithful application, there are different approaches in the current hermeneutical/homiletical theories for sermon application. In this regard, this paper suggests that the preacher need a ‘biblically appropriated application’ for more text-faithful preaching in consideration of the author’s designed textuality. Accordingly, this study first provides the foundational concept of biblical appropriation, examining the explanations of two famous philosophical hermeneuticians, Hans-Georg Gadamer and Paul Ricoeur. And from the perspective of proper understanding of biblically appropriated application, this study evaluates the paradigms both of traditional expository preaching and the New Homiletic. Futhermore, this paper probes for an integrated model with the concept of ‘biblically appropriated application,’ and provides its homiletical implications. 회중의 삶을 변화시키는 성경적 설교는 성경 본문의 의미를 바르게 설명하고, 오늘을 살아가는 회중에게 그 의미가 제대로 적용되도록 해야 한다. 여기에 본문에 충실한 적용은 성경적 설교자에게 매주 되돌아오는 필수적인 과제이자 책임이다. 하지만 본문에 충실한 적용을 동일하게 지향함에도, 실제 해석학적/설교학적 상이한 모델들이 제시되고 있다. 이런 맥락에서 이 논문은 설교자가 저자가 구현한 본문성을 충실히 고려하여 설교의 적용을 수행하기 위해 성경적 전유를 통한 적용의 필요성을 제안하고자 한다. 이를 위해서 우선 성경적 전유의 개념을 철학적 해석학자들인 Hans-Georg Gadamer와 Paul Ricoeur의 해석 이론들을 통해서 살펴보고 그 공통점을 확인해본다. 이런 해석학적 이해를 바탕으로, 성경적 전유를 통한 적용의 관점에서 전통적 강해설교와 신설교학의 장단점을 평가해 보고, 성경적 전유를 통한 적용으로의 통합적인 발전을 모색해본다. 끝으로 이 논문은 성경적 전유를 통한 적용이 가져다주는 설교학적 함의들을 제안한다.

Expression of Fungal Phytase on the Cell Surface of Saccharomyces cerevisiae

김대혁,박승문,양문식,Ae-Young Mo,Yun-Sik Kim 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.6

Phytase improves the bioavailability of phytate phosphorus in plant foods to humans and animals, and reduces the phosphorus pollution of animal waste. We have engineered the cell surface of the yeast, Saccharomyces cerevisiae, by anchoring active fungal phytase on its cell wall, in order to apply it as a dietary supplement containing bioconversional functions in animal foods and a whole cell bio-catalyst for the treatment of waste. The phytase gene (phyA) of Aspergillus niger with a signal peptide of rice amylase 1A (Ramy1A) was fused with the gene encoding the C-terminal half (320 amino acid residues from the C-terminus) of yeast a-agglutinin, a protein which is involved in mating and is covalently anchored to the cell wall. The resulting fusion construct was introduced into S. cerevisiae and expressed under the control of the constitutive glyceraldehydes-3-phosphate dehydrogenase (GPD) promoter. Phytase plate assay revealed that the surface-engineered cell exhibited a catalytically active opaque zone which was restricted to the margin of the colony. Additionally, the phytase activity was detected in the cell fraction, but was not detected in the culture medium when it was grown in liquid. These results indicate that the phytase was successfully anchored to the cell surface of yeast and was displayed as its active form. The amount of recombinant phytase on the surface of yeast cells was estimated to be 16,000 molecules per cell.