http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
김기청,이웅,송동업,조백호,Kim, Ki-Chung,Lee, Ung,Song, Dong-Up,Cho, Baik-Ho 한국식물병리학회 1996 Plant Pathology Journal Vol.12 No.1
3종의 빙핵세균 Peudomonas syringae 8401, Pseudomonas fuorescens 8701, Erwinia herbicola 8701의 세포 외막으로부터 아무런 변성제도 사용치 않고 sucrose density gradient centrifugation, Sephacryl gel filtration chromatography, DEAE-cellulose ion exchange chromatography, non-denaturing buffer를 이용한 PAGE, electroelution, SDS-PAGE를 통해 빙핵활성 단백질을 고도로 정제할 수 있었다. P. suringae와 P. fluorescens에서는 각각 3종류(155 kD, 75 kD, 50 kD)의 빙핵활성 단백질이, E. herbicola에서는 155 kD를 제외한 2종류(75 kD, 50 kD)의 빙핵활성 단백질은 이 연구를 통해 처음 밝혀진 것으로 , 지금까지 보고된 빙핵활성 단백질(150 KD 이상)보다는 훨씬 작은 것이다. 이는 빙핵활성을 나타내는 단백질의 기본단위는 이 실험의 결과만에 의하면 최대 50 kD임을 시사한다. 이들 단백질은 그 유래된 세균의 종류나 또는 단백질 분자량의 크기에 관계없이 모두 -5.5~7.5$^{\circ}C$에서 물을 동결시키는 높은 빙핵활성을 갖고 있었다. 이는 지금까지 보고된 어느 정제단백질의 빙핵활성보다 높은 것이다. 정제된 단백질의 빙핵활성은 trypsin 처리에 의해 상실되었고, pH6~8범위에서는 안정하였으며, pH5이하, pH9이상에서는 활성을 상실하였다. 보존온도에 대한 영향은 3$0^{\circ}C$이상이 되면 점차 활성이 감소하는 경향을 보이다 37$^{\circ}C$이상에서는 활성이 완전히 상실되었다. 금속이온으로서 Hg\ulcorner이온과 SDS에 의해 활성이 상실되었으나 phosphatidylinositol의 첨가에 의해서는 활성이 약간 증가(-1$^{\circ}C$)하였다.
흰가루병균 분생포자 ethanol 추출분획의 대맥엽 형광화세포 유도활성
김기청,사산자효,Kim Ki Chung,Shishiyama Jiko 한국응용곤충학회 1979 한국식물보호학회지 Vol.18 No.4
보리흰가루병(Erysiphe graminis hordei race I)에 의한 대맥엽침입부에 항균성 형광화세포를 유도하는 물질의 추출분획을 얻기 위하여 실험을 실시한 결과 분생포자의 ethanol 추출분획이 형광화세포의 유도활성을 가지고 있음이 밝혀졌다. 이 분획은 비친화성인 Turkey 290품종에나 친화성품종에나 마찬가지로 형광화세포 유도활성을 나타냈으나 그 유도에 요하는 시간은 Turkey 290에서 8시간 이내 Kobingataki에서 16시간이내이었다. The autofluorescent cells in the penetration area of powdery mildewed leaves of barley had been reported. The present experiments were performed in order to obtain the fluorescence-inducing extract fraction from the conidia. The preparations were made by extractions and residue which were extracted from the conidia of Erysiphe graminis hordei race I with water, ethanol, and ethyl ether. Bioassaying was carried out on the culled-leaf surface of incompatible Turkey 290 and compatible Kobingataki varieties by placing the drops of extract solutions. Fluorescence-elicitor activity was shown only in tile ethanol-extract fraction to both varieties. However, fluorescence-eliciting rate was more rapid on the leaves of incompatible variety Turkey 290 than on tile those of compatible variety Kobingataki; Turkey 290 less than 8 hours, Kobingataki less than 16 hours.
아카시아흰가루병균의 완전시대 및 Erysiphe 층과의 계통관계
김기청 ( Ki Chung Kim ) 한국산림과학회 1969 한국산림과학회지 Vol.9 No.1
In present paper, the morphological characters in perfect stage of Microsphaera polygoni(DC.) Sawada on Robinia pseudoacasia were investigated and the phylogenetic relationship between Gen. Erysiphe and Microsphaera was discussed with variation of appendages. The results are summarized as follows: 1. The perithecia of the powdery mildew fungus on Robinia pseudoacasia were rarely formed on the surface of the leaves just before difoliation in late autumn and their forming period was very short. 2. Powdery mildew fungus on R.pscudoacasia was identified as Microsphaera polygoni(DC.) sawada in Korea. 3. Appendage of the fungus are both Erysiphe and Microsphaera ytpes in shape, and plenty of interealary types are intervened between both types. Number of perithecia bearing upper various appendages appears the normal distribution with both poles of typical Erysiphe and Microsphaera types. 4. If Blumer`s theory on the phylogenetic relationship of Erysi phaceae is right, variation of the appendages of the fungus might be evolved from Gen, Erysiphe to Gen, Microsphaera.
이웅,권미경,성기영,조백호,김기청,Lee, Ung,Kwon, Mi-Kyung,Seong, Ki-Young,Cho, Baik-Ho,Kim, Ki-Chung 한국식물병리학회 1999 식물병연구 Vol.5 No.1
Frost injury of crops is closely related to the epiphytic population dynamics of ice nucleation-active (INA) bacteria, and the injury can be reduced by decreasing the INA bacterial population. In order to predict the epiphytic population of INA bacteria on crops, a rapid and accurate detection method has to be developed. In the previous report, we produced some antibodies against INA proteins purified from the outer membrane of INA bacteria. However it was difficult to produce the antibodies because the purification procedures of the INA proteins were complicated, and the final yield was too low. We designed a specific peptide from the N-terminal region of INA protein by computer analysis and synthesized the peptide in vitro in this experiment. The peptide sequence was Asp-Ser-Por-Leu-Ser-Leu-His-Ala-Asp, that is corresponding to the highly conserved region in several INA proteins, with predicted beta turn, coiling, and hydrophilic region. A polyclonal anti-INA peptide antiserum produced specifically recognized INA bacteria as few as 10 colony-forming units (CFU) in the ELISA reactions and did not respond to other non-INA bacteria. Serological specificity of the anti-INA peptide antiserum will facilitate the forecasting of the INA bacterial population dynamics on crops.