Privatization of the Saemaul Movement

김기옥 ( Kee Ko Kim ) 한국지방행정연구원 1992 地方行政硏究 Vol.7 No.4

과거 3년간 본교실에서 행한 병리조직학적검사 704예에 대한 통계적 고찰

김경자(KJ Kim),김기옥(KO Kim),안재용(JY Ahn),이원배(WB Lee) 대한산부인과학회 1966 Obstetrics & Gynecology Science Vol.9 No.10

Stastistical analysis of 704 biopsies which were obtained by punch biopsy, curettage&surgical specimens froms the pt`s in our fields during the past 3 years at our department of Pusna National University Hospital was done. Obtained results were as follows; 1. Of the 704 specimens, malignant tumor was 11.93%, benign tumor 3.7%, inflammation 41.8%, endometral hyperplasia 14.2%, & atrophic endometrium 22.2%. 2. Of the 294 inflammation cases, cervicitis was 73.1%, tuberculous endometritis 3.8%, tuberculous salpingitis 8.3%. 3. Of the 116 tumor cases, malignant tumor was 71.5%& of the 86.7% was orginated from the cervix. Malignant tumous were composed mainly 74.3%(62 cases)of squamous cell carcinoma, 1 case of adenocarcinoma & 1 cases of adenocarcinoma of endometrium. 4. Glandular hyperplasia was 94 cases (45.1%) & atrophic endometial pattern cases (7.2%) & normal endomerial pattern 93 cases(44%).

비목나무 (Lindera erythrocarpa Makino) 껍질에서 분리한 신규화합물 (Jeju-Erythrane)의 멜라닌 생합성 억제 효과

강민철(Min-Chul Kang),고려경(Ryeo Kyeong Ko),김수경(Su-Gyeong Kim),최호민(Ho-Min Choi),진영준(Yeong-Jun Jin),한종헌(Jong-Heon Han),김봉석(Bong-Seok Kim),이남호(Nam Ho Lee),김기옥(Gi-Ok Kim) 한국생물공학회 2010 KSBB Journal Vol.25 No.4

본 연구는 제주도에서 자생하는 비목나무 (Lindera erythrocarpa) 껍질에서 분리 · 동정한 신규화합물 (Jeju-Erythrane)의 항산화 활성 및 B16F10 mouse melanoma 세포에서의 tyrosinase와 멜라닌 생합성의 억제효과를 확인하여, 유용자원으로서 미백 및 기능성 화장료로의 산업적 활용 가능성이 있는지 알아보고자 시행하였다. 실험 결과, 신규화합물인 Jeju-Erythrane의 항산화 활성은 확인되지 않았고, B16F10 세포에서 tyrosinase와 멜라닌 합성을 농도 의존적으로 억제하는 것으로 밝혀졌으며, tyrosinase와 TRP-1 유전자의 단백질 및 mRNA 발현양도 농도 의존적으로 감소함을 보였다. 하지만, TRP-2 단백질과 mRNA는 농도에 관계없이 일정하게 발현하는 것을 알 수 있었다. 따라서, 본 연구결과는 비목나무 (Lindera erythrocarpa) 껍질에서 분리 · 동정한 신규화합물 (Jeju-Erythrane)이 미백 관련 기능성 소재로서의 활용가치가 있음을 보여준다. In this study, a new compound, 1-(2-hydroxy-3,4,5,6-tetramethoxyphenyl)-1-methoxy-3-phenylpropane; (Jeju-Erythrane) was isolated and identified from the bark of Lindera erythrocarpa Makino. Also, we investigated the effects of Jeju-Erythrane on alpha melanocyte-stimulating hormone (MSH)-induced melanogenesis in mouse B16F10 melanoma cells. The new compound dose dependently inhibited the tyrosinase activity and melanin synthesis in B16F10 cells. The new compound showed inhibitory effect on the Tyrosinase and TRP-1 gene transcription but not on the TRP-2 gene. These results suggest that the new compound of L. erythrocarpa could be used as a functional biomaterial in developing skin whitening agent.

본교실에서 최근 2년간에 실시한 자궁질세포 도말표본의 검사

서옥자(OJ Suh),김경자(KJ Kim),강대식(DS Kang),김기옥(KO Kim) 대한산부인과학회 1966 Obstetrics & Gynecology Science Vol.9 No.10

Authors performed analysis of vaginal smear of total 859 out patient at Dept. of Obstet. & Gynec. of Pusan National University between Sept. 1st, 1963 to Aug. 31st, 1965. This paper is consecutive study following previous report by Chun Taek Ro, titled as "Statistical Studies of Cervical Cancer for five years and following conclusions were made by clinico-cyto-histological findings. 1. Positive smear cases of parity was dominant at para 4 as 20.3 percent. 2. In chief complaint of patient, leukorrhea is 28 percent, genital bleeding is 26.8 percent, bloody leukorrhea is 19.3 percent, 3. Age of positive smear is 41-50(37.6 percent,), 31-40(34.4 percent). 4. Erosion of uterine portio was divided into 3 forms, papillary erosion is 33.4 percent, simple erosion is 10.75 percent and follicle erosion is 3.32 percent. Other percent was erosion negative and other abnormal form of uterine portio. 5. All of pregnancy cases is negative smear. Thus we didn`t analysis for positive smear in pregnancy. 6. Positive smear of abortion history was total 40 cases and artificial abortion cases were more higher than spontaneous abortion(27 to 13). 7. In compare with cytological and histological diagnosis, a ccurate diagnosis between them 94.8 percent, and result was obtained as non-cancer patient has 3.3 percent of false negative and cancer patiaent has 6.79 percent of false positive. 8. In error between clinical diagnosis and cytological diagnosis, positive is 33, doubtful is 71. 9. In the cytological diagnosis, among 859 cases, negative 66.6 percent, doubtful 22 percent and positive 10.78 percent.

미분화세포종의 1예

서옥자(OJ Suh),김경자(KJ Kim),김기옥(KO Kim),이문옥(MO Lee) 대한산부인과학회 1966 Obstetrics & Gynecology Science Vol.9 No.10

A case of dysgerminoma of left ovary in 31 years old woman is reported. And the literatures on dysgerminoma of the ovary is reviewed briefly.

제주도 근해에 자생하는 참가죽그물바탕말 (Dictyota coriacea) 추출물의 멜라닌 억제 효과 및 항염증 효과

강민철(Min-Chul Kang),이주엽(Ju-Yeop Lee),고려경(Ryeo Kyeoung Ko),김행범(Haeng-Bum Kim),홍승호(Seung-Ho Hong),김기옥(Gi-Ok Kim) 한국생물공학회 2008 KSBB Journal Vol.23 No.4

제주도 자생 제주아그배 (Malus micromalus Makino) 추출물의 항산화와 항염증 활성

이주엽(Ju-Yeop Lee),강민철(Min-Chul Kang),이정아(Jung-A Lee),고광효(Kwang-Hyo Ko),김봉석(Bong-Seok Kim),한종헌(Jong-Heon Han),김세재(Se Jae Kim),김기옥(Gi-Ok Kim) 한국생물공학회 2009 KSBB Journal Vol.24 No.4

본 연구는 제주에 자생하고 있는 제주아그배의 생리활성, 즉 항산화 효과와 항염 효과를 알아보고자 수행하였다. 제주 아그배의 항산화 활성을 측정하고자 비교 분석한 결과, 제주 아그배 에탄올 추출물 (DPPH 소거활성 IC50 값, 16.1 μg/mL; NO 소거활성 IC50 값, 901.2 μg/mL; superoxide 소거활성 및 Xanthine oxidase 억제 활성 IC50 값, 42.5 μg/mL, 2.7 μg/mL) 은 좋은 항산화 활성을 보였고, 그 분획물 중에서는 에틸아 세테이트 분획물이 항산화 활성 실험 모두에서 높은 소거 활성을 나타내었다. 따라서 항산화 활성이 좋은 제주아 그배의 추출물의 항염증 효과를 탐색하기 위하여 대식 세포 모델을 이용하였다. LPS로 자극한 마우스 대식세포인 RAW 264.7 세포에서 NO 활성과 세포독성간의 상대적 비교지수인 선택지수를 이용하여 탐색한 결과 에탄올 추출물과 에틸아세테이트 분획물이 2.9와 12.2로 가장 높은 상대적 NO 생성 억제 활성을 보였다. 그리고 NO 생성 억제율에 대한 IC50 값은 각각 16.7 μg/mL, 4.1 μg/mL으로 나타났다. 이런 결과를 기초로 하여 제주아그배의 에탄올 추출물의 NO 감소가 iNOS 단백질 감소에 의한 것임을 확인한 결과, 시료를 처리한 RAW 264.7 세포에서 iNOS 단백질이 농도 의존적으로 감소하였다. 또한 iNOS 단백질의 억제가 mRNA의 감소로 인한 것인지 조사하고자, RT-PCR 을 통해 iNOS 의 mRNA 발현을 조사한 결과, 단백질과 마찬가지로 농도 의존적인 mRNA 발현 감소를 볼 수 있었다. 제주아그배의 에탄올 추출물을 농도 별로 처리하였을 때 유의적으로 COX-2 단백질과 mRNA 발현이 농도 의존적으로 억제하였다. 분획물 중에서는 NO 활성과 세포 독성간의 상대적 비교지수인 선택지수가 높았던 에틸아세테 이트 분획물이 에탄올 추출물보다도 NO, iNOS, COX-2의 억제 정도가 더 좋은 경향을 나타내었다. The antioxidant and anti-inflammatory activities of ethanol extract of Malus micromalus were studied in vitro. Ethanol extract of M. micromalus showed scavenging effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO) radicals. In addition, ethanol extract of M. micromalus inhibited the generation of superoxide anion (O2 -) radical and uric acid by xanthine oxidase. We also investigated the effect of ethanol extract of M. micromalus on NO production in a lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells. Ethanol extract of M. micromalus significantly inhibited NO production and this inhibitory action was not due to the cytotoxicity. The expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was markedly down-regulated by ethanol extract of M. micromalus. These results indicate that the inhibitory action of ethanol extract of M. micromalus on NO production in LPS-stimulated macropages might be due in part to abrogation of iNOS and COX-2 protein induction. Taken together, this study suggests that ethanol extract of M. micromalus could contribute to the chemoprevention and therapy of oxidative stress and inflammation.

제주도 재래종말에서 혈액 단백질의 유전적 변이에 관한 연구

김세재,정창조,오문유,김규일,김기옥,고미희 濟州大學校 基礎科學硏究所 1992 基礎科學硏究 Vol.5 No.1

In order to clarify the genetic composition and to find possible genetic markers of Cheju native Horses, the genetic variations of the nine proteins (Al, Pi, Es, Hp, Hb, PGD, PGM, MDH, ME) were investigated by the gel electrophoretic techniques. Al: there were two alleles, AlA and AlB. The alleles frequencies of AlA and AlB were 0.398 and 0.602, respectively(X²=0.02, d.f.=2, P>0.9). Pi: Seven Pi alleles(PiF, PiG, PiI, PiL, PiN, PiS and PiU) were detected. The gene frequencies of Pi were PiG 0.136, PiL 0.496, PiN 0.260, and other(PiF, PiI, PiU, PiS) 0.108, respectively(X²= 62.48, d.f.=12, P<0.005). Es: There were three alleles, EsF, EsI and EsS. The frequencies of Es were EsF 0.266, EsI 0.712, and EsS 0.022, respectively(X²= 21.28, d.f.=3, P<0.005). Hb: Three alleles (HbA, HbBI, and HbBII) were found. The gene frequencies of Hb were HbA 0.120, HbBI 0.620 and HbBII 0.260, respectively(X²=2.63, d.f.=3, P>0.25). PGM: There were two alleles, PgmF and PgmS The gene frequencies of PGM were PgmF 0.434 and PgmS 0.566, respectively(X²=0.22, d.f.=2, P>0.75). PGD: There were two alleles, PgmF and PgmS. The gene frequencies of PGD were PgmF 0.823 and PgmS 0.177 respectively(X²=7.53, d.f.=2, P<0.05). Three proteins (Hp, MDH, ME) showed the monomorphic traits in this population. The mean heterozygosity of nine protein loci were calculated as 0.329±0.012.

제주도 재래종말에서 혈액 단백질의 유전적 변이에 관한 연구

김규일,정창조,김세재,오문유,고미희,김기옥 한국유전학회 1992 Genes & Genomics Vol.14 No.1

In order to clarify the genetic composition and to find possible genetic markers of Cheju native horses, the genetic variations of the nine proteins(Al, Pi, Es, Hp, Hb, PGD, PGM, MDH, ME) were investigated by the gel electrophoretic techniques. Al: There were two alleles, Al^A and AI^B. The alleles frequencies of AI^A and Al^B were 0.398 and 0.602, respectively(X²= 0.02, d.f.=2, P>0.9). Pi: Seven Pi alleles (Pi^F, Pi^G, Pi^I, Pi^L, Pi^N, Pi^S and Pi^U ) were detected. The gene frequencies of Pi were Pi^G 0.136, Pi^L 0.496, Pi^N 0.260, and others(Pi^F, Pi^I, Pi^U, Pi^S) 0.108, respectively(X²= 62.48, d.f.=12, P<0.005). Es: There were three alleles, Es^F, Es^I and Es^S. The gene frequencies of Es were Es^F 0.266, Es^I 0.712, and Es^S 0.022, respectively(X²= 21.28, d.f.=3, P<0.005). Hb: Three alleles (Hb^A, Hb^(BI) and Hb^(BII)) were found. The gene frequencies of Hb were Hb^A 0.120, Hb^(BI) 0.620 and Fb^(BII) 0.260, respectively(X²=2.63, d.f.=3, P>0.25). PGM: There were two alleles, Pgm^F and Pgm^S. The gene frequencies of PGM were Pgm^F 0.434 and Pgm^S 0.566, respectively (X²= 0.22, d.f.=2, P>0.75). PGD: There were two alleles, Pgd^F and Pgd^S. The gene frequencies of PGD were Pgd^F 0.823 and Pgd^S 0.177, respectively(X²= 7.53, d.f.=2, P < 0.05). Three proteins (Hp, MDH, ME) showed the monomorphic traits in this population. The mean heterozygosity of nine protein loci were calculated as 0.329±0.012.

벗나무속 수종간의 핵 리보솜 DNA의 ITS Ⅰ 염기서열 비교

오유성,김기옥,정용환,오문유,한상현,고미희 제주대학교 생명과학연구소 1998 제주생명과학연구 Vol.1 No.-

Comparison of four species (Prunus yedoensis, P. sargentii, P. pendula for. ascendens, P. serrulata var. quelpaertensis) and one cultivar of P. yedoensis were carried out by comparing their nucleotide sequences from the internal transcribed spacers (ITS) I of nuclear ribosomal DNA (rDNA). The length of the ITS I region was ranged from 229 to 236 base pairs (bp). The G+C contents of ITS I ranged from 58.5 to 60.2%. The value of nucleotide divergence across for four Prunus species and one cultivar ranged from 0.4 to 6%. The pairwise distance between P. pendula for. ascendens and P. yedoensis was 0.004 showing the lowest value among any other pairs. On the other hand, the pairwise distance betwen P. yedoensis and cultivated P. yedoensis was 0.026. The nucleotide analysis based on ITS I sequence was considerably similar to previous studies on the basis of random amplified polymorphic DNA (RAPD).