Expression and Purification of Human Papillomavirus 18 L1 Virus-Like Particle from Saccharomyces cerevisiae

Mi-Kyung Woo,Jun-Dong Kim,Jung-Mo An,Sue-Nie Park,김홍진 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.2

Cervical cancer caused by human papillomavirus (HPV) might be successfully prevented by HPV vaccination and screening. HPV vaccination and HPV serology assays have been investigated using HPV virus-like particles (VLPs). In this study we produced HPV18 L1 VLPs in Saccharomyces cerevisiae and purified them. The HPV18 L1 gene was cloned into the yeast expression vector YEGα-HIR525, and transformed into Saccharomyces cerevisiae. Expression of HPV18 L1 protein was demonstrated by Western blotting. The HPV18 L1 protein was purified by ultracentrifugation, size-exclusion chromatography and cation-exchange chromatography, and was up to 95% pure. We showed by transmission electron microscopy that the purified protein self-assembled into VLPs. These findings should be useful for establishing vaccine efficacy as well as characterizing vaccine candidates, and may provide an international reference standard for HPV serology assays.

여대생의 월경 태도, 월경 전 불편감과 월경 전 대처와의 관계

한지희 ( Han Jee Hee ),김수 ( Kim Sue ),김상희 ( Kim Sanghee ),이선경 ( Lee Sun Kyung ) 여성건강간호학회 2017 여성건강간호학회지 Vol.23 No.1

Purpose: This paper presented a descriptive study which aimed to identify the relationship amongmenstrual attitude, premenstrual distress and premenstrual coping in Korean college women and to propose fundamental data for the development of guidelines for effective management of premenstrual distress. Methods: The data were collected from 111 college women and analyzed using descriptive statistics, t-tests, ANOVA and Pearson`s correlation coefficients. Results: The mean score of premenstrual coping was 3.25±0.60 out of 5 points. The `Awareness and Acceptance of Premenstrual Change` score was highest and the `Avoiding Harm` score was lowest. Menstrual attitude was significantly related to premenstrual distress (r=.37 p<.001) and the awareness and acceptance style of premenstrual coping (r=-.21. p=.021). And premenstrual distress was significantly related to the self-care coping style of premenstrual coping (r=.30, p=.001), the adjusting energy coping style of premenstrual coping (r=.45, p<.001) and the avoiding harm coping style of premenstrual coping (r=.48, p<.001). Conclusion: Results of this study support that positive attitude about menstruation is necessary for the effective management of premenstrual distress. Also, premenstrual coping programs that consider the level of premenstrual distress will be needed for college women.

소아 무균성 뇌막염의 역학적 연구를 위한 건강보험자료원의 유용성 평가

박수경,기모란,손영모,김호,정해관,Park, Sue-Kyung,Ki, Mo-Ran,Son, Young-Mo,Kim, Ho,Cheong, Hae-Kwan 대한예방의학회 2003 Journal of Preventive Medicine and Public Health Vol.36 No.4

Objectives : Aseptic meningitis is a major cause of Korean childhood morbidity late spring and early summer. However, the nationwide incidences of the disease have not been reported. This study was conducted to evaluate the availability of National Health Insurance data (NHID) for the study of an epidemiological trend in the surveillance of aseptic meningitis in children. Methods : All the claims, under A87, A87.8, and A87.9 by ICD-10, among children below 15 years of age, to the National Health Insurance Corporation, between January and December 1998, were extracted. A survey of the medical record of 3,874 cases from 136 general hospitals was peformed. The availability of the NHID was evaluated by the three following methods: 1) The diagnostic accuracy (the positive Predictive value : proportion of the confirmed aseptic meningitis among the subjects registered as above disease-codes in NHID) was evaluated through a chart review, and according to age, gender, month and region of disease-occurrence. 2) The distribution of confirmed cases was compared with the distribution of total subjects from the NHID, for subjects in General hospitals, or the subjects surveyed. 3) The proportion of confirmed CSF test was confirmed, and the relating factor, which was the difference in CSF-test rate, analyzed. Results : Among 3,874 cases, CSF examinations were peformed on 1,845 (47.6%), and the CSF-test rates were different according to the medical utility (admission vs. OPD visit) and the severity of the symptoms and signs. The diagnostic accuracy for aseptic meningitis, and during the epidemic (May-Aug) and sporadic (Sept-Apr) periods, were 85.0 (1,568/1,845), 86.0 (1,239/l,440) and 81.2% (329/405), respectively. The distributions by age, sex, month or period (epidemic/sporadic) and region, in the confirmed cases, were similar to those in the NHID, in both the subjects at General hospitals and in those surveyed, to within ${\pm}7%$. Conclusions : In this paper, the NHID for the subjects registered with an aseptic meningitis disease-code might be available for an epidemiological study on the incidence-estimation of childhood aseptic meningitis, as the NHID could include both the probable and definite cases. On the basis of this result, further studies of time-series and secular trend analyses, using the NHID, will be peformed.

열공성 뇌경색 환자-대조군에서 신경행동검사의 수행 평가

김함겸,박수경,이건세,김형수,김화선,장성훈,Kim, Ham-Gyum,Park, Sue-Kyung,Lee, Kun-Sei,Kim, Hyeoug-Su,Kim, Wha-Sun,Chang, Soung-Hoon 대한예방의학회 2003 예방의학회지 Vol.36 No.3

전체 뇌졸중의 $15{\sim}20%$를 차지하고 있는 열공성 뇌경색(lacunar infarction)은 최근 뇌경색형 뇌졸중의 증가와 더불어 그 중요성이 점차 증가하고 있는데, 1년 이내 뇌경색이 재발되거나 노인, 고혈압 및 초기 신경학적 이상과 같은 감수성 인자가 있는 경우 뇌경색 재발과 혈관성 치매 발생 위험이 있어 삼차예방적 측면에서의 적절한 처치가 필요한 질병이다. 그렇지만 열공성 뇌경색은 증상이 없거나 경미한 경우가 상당히 많아 환자 자신이 치료의 중요성을 느끼지 못할 뿐 아니라 의사들도 환자의 경미한 신경학적 이상을 인지하지 못하여 처치의 당위성에 대해 설득력있는 근거를 제시할 수 없어 적절한 처치가 미비한 상황이다. 본 연구는 신경학적 자각 증상은 없지만 MRI상 열공성 뇌경색으로만 진단된 환자군이 MRI상 이상소견이 관찰되지 않는 대조군에 비하여 신경행동 검사상 차이가 관찰될 것이라는 가설을 구명하고자 하였다. 따라서 뇌졸중의 증상 및 증후 없이 두개골 외상 혹은 두통, 경부강직 등과 같은 신경학적 이상으로 MRI를 촬영한 대상자 중에서 다른 이상 없이 열공성 뇌경색으로만 진단될 환자군과 특별한 이상 소견이 없는 것으로 진단된 대조군에서 WHO의 NCTB(neurobehavioral core test battery)검사의 일부와 Perdue pegboard score검사를 시행하였고, 그 결과 대조군에 비해 환자군의 신경행동 이상이 평균 및 가장 빠른 단순반응시간 검사를 제외한 5개 검사에서 관찰되었다. 본 검사의 결과는 자각 증상이 없거나 경미한 증상을 호소하는 열공성 뇌경색 환자들의 차후 필요한 검사의 선별과 삼차 예방적 측면에서 처치의 근거로서 활용될 수 있을 것이다. 또한 자각 증상이 없거나 경미한 증상이 있지만 진찰상 신경학적 이상 증후를 거의 찾을 수 없는 대상자에서 MRI검사 필요성의 근거를 확인하고자 하는 대상자에게 이용될 수 있을 것으로 사료된다. Objectives : We carried out tests for neurobehavior by using WHO-NCTB (neurobehavioral core test battery) and Perdue pegboard score test to identify differences between lacunar infarction cases and controls. Methods : Among the subjects who underwent MRI between February 2001 and March 2002 in a university hospital located in Seoul and who were diagnosed only as lacunar infarction without any intracranial disease, 46 patients were selected as cases (male: 21, female: 25). Controls were selected who had no cerebrovascular disease on MRI by matching age (5 years), gender, and education (2 years) in a ratio of 1:1 , Among WHO-NCTB, the following 5 tests and Perdue pegboard score test were used to categorize the study subjects: digit and symbol matching, simple reaction time, Benton visual retention, digit span, and Pursuit aiming test, Results : Among the above 6 tests of neurobehavior, lacunar infarction cases showed lower score than controls except for the simple reaction time test. As the controlling variables of multivariate analysis in the stepwise regression analysis, the followings were selected due to their significant association: age, education, BMI, gender, drinking, exercise, add systolic blood pressure. From multivariate regression analysis, there was significant difference (p<0.05) between lacunar infarction cases and controls in digit and symbol matching, Benton visual retention, digit span, pursuit aiming, and Perdue pegboard score test, but not in the score of simple reaction time test. Conclusions : We suggest that the above 5 tests for neurobehavior, with the exception of the simple reaction time test, might be used as the basis for recommendation of further treatment and other neurological tests by the earlier defection for neurological abnormality in lacunar infarction.

2세포기로의 조기난할 배아 선발을 이용한 체외수정술의 임신율 증가

박세희,주보선,이수경,김경서,문화숙,Park, Sea Hee,Joo, Bo Sun,Lee, Su Kyung,Kim, Kyung Sue,Moon, Hwa Sook 대한생식의학회 2005 Clinical and Experimental Reproductive Medicine Vol.32 No.1

Objective: Evaluation of embryos using early cleavage to 2-cell stage has been proposed, but a critical time-point for selecting embryos is unclear. The aim of the present study is to provide a guideline including critical time-point in the selection of early cleaving embryo for the reduction of multiple pregnancies as well as the increase of pregnancy rate in human IVF. Methods: This prospective study was performed in 116 cycles from 85 patients who underwent conventional IVF or ICSI at the infertility clinic of Good Moonhwa Hospital from January 2002 to December 2003. Early cleavage (EC) of embryos to 2-cell stage was assessed at 25 h and 27 h postinsemination/microinjection. Embryos that had early cleaved at each time point were designated as EC-1 and EC-2, respectively, while others were designated as non-early cleavage (NEC). Results: At least one early cleavage embryo was observed in 54 (46.6%) for the EC-1 and 84 (72.4%) for the EC-2 of the 116 cycles assessed. Clinical pregnancy rates (PR) were significantly higher in the EC-1 group (66.7%) compared to the EC-2 group (53.6%) or the NEC group (31.2%) (p<0.05). Significant improvement of the pregnancy rate was found when at least two or more embryos were early cleaved at 25 h postinsemination or when the proportion of early cleavage embryo at 25 h postinsemination was higher than 20% (p<0.05). Conclusion: The critical time-point for the selection of early cleavage embryos with high implantation potential is more effective in 25 h postinsemination/microinjection compared to 27 h. The proportion as well as number of early cleavage embryos is also an important factor for the prediction of pregnancy outcome and the chance of multiple pregnancies. These results demonstrated that the evaluation of early cleavage embryos to 2-cell stage is an easy, simple, and objective method for the selection of good quality embryos suitable for embryo transfer.

일반인의 에이즈에 대한 지식 및 태도

김소영(So Young Kim),이진석(Jin Seok Lee),박수경(Sue Kyung Park),김용익(Yong Ik Kim) 한국보건교육건강증진학회 2008 보건교육건강증진학회지 Vol.25 No.4

정신질환에 대한 일반인의 사회적 거리감에 영향을 미치는 요인

문상준,이진석,박수경,이선영,김윤,김용익,신영수,Moon, Sang-Jun,Lee, Jin-Seok,Park, Sue-Kyung,Lee, Sun-Young,Kim, Yoon,Kim, Yong-Ik,Shin, Young-Soo 대한예방의학회 2008 예방의학회지 Vol.41 No.6

Objective: The purpose of this study was to investigate impact of knowledge familiarity, and prejudice about mental illness as well as demographic factors on the social distance from mentally ill people, which is a proxy measure of discrimination. Method: To assess the impact of knowledge and familiarity, prejudice about mental illness and demographic factors on the social distance from mental illness, we conducted a telephone survey in South Korea with the responders being nationally representative people who were 18 years old or over (n=1040). Independent samples T-tests, one way ANOVA and linear regression analysis were performed to analyze the results of the survey. Result: The social distance from mental illness decreased as the knowledge and familiarity increased, but the social distance was increased as prejudice was increased. Prejudice had a greater impact on social distance than familiarity and knowledge. Females showed greater social distance than did males. A higher education level had a negative effect on social distance. Conclusion: to reduce the social distance from mentally ill people, efforts to increase the familiarity about mental illness as well as efforts to educate people about mental illness are important.

생쥐에 대한 생모수의 육모효과

최설민(Seul Min Choi),김대영(Dae Young Kim),박요안(Yo An Park),이미경(Mi Kyung Lee),송현수(Hyun Sue Song),권오성(Oh Sung Kwun),임승용(Seung Yong Lim),배혜진(Hye Jin Pae),신동훈(Dong Hun Shin),김주영(Ju Young Kim),김형식(Hyung Sik Kim 대한약학회 1999 약학회지 Vol.43 No.3

Hair growth effects of Sangmosu have been evaluated in C57BL/6 mice. Animals were topically treated with Sangmosu (50 or 100mcl/mouse) for 40 days on the back of shaved area. Twenty days after treatment, hair growth effects were significantly observed in both female and male mice. There was no sex difference although 30 days after treatment female mice were shown to be more effective at the dose of 50mcl/mouse than males. These data demonstrate that Sangmosu is very effective in hair growth effects in animals and thus it may be applicable to humans.

Polyoma Virus Nephropathy로 이식신 소실 후 시행한 사체 재이식

전지민 ( Jee Min Jeon ),김지환 ( Ji Hwan Kim ),박미정 ( Mi Jung Park ),박창수 ( Chang Sue Park ),김성민 ( Sung Min Kim ),오혜주 ( Hyae Joo Oh ),박용기 ( Yong Kee Park ),신용훈 ( Yong Hun Shin ),김중경 ( Joong Kyung Kim ),허길 ( K 대한신장학회 2005 Kidney Research and Clinical Practice Vol.24 No.3

대중교통 내 교통약자와 비교통약자 간의 의사소통 서비스 디자인 제안교통 : 약자 중에서도 임산부를 대상으로

신예진(Shin,YeJin),송진경(Song,Jin Kyung),정가람(Jeong, Ga Ram),김다운(Kim, Daun),박수이(Park, Sue) 한국디자인리서치학회 2019 한국디자인리서치 Vol.4 No.1

교통약자 중에서도 임산부는 대중교통 수단의 이용이 어려운 승객 중 하나이다.본 연구의 목적은 대중교통 내 교통약자의 배려를 위한 교통약자와 비교통약자의 간의 원활한 의사소통을 위한 의사소통 서비스 디자인을 제안하고자 한다.이를 위하여 사전 연구에서는 임산부를 대상으로 설문과FGI를 진행하였다.연구1에서는 앞서 진행한 사전연구의 결과를 바탕으로 모바일 기반의 교통약자와 비교통약자 간의 의사소통 서비스를 실제로 구현하였다. 마지막으로 연구 2에서는 구현한 결과물을 실제 지하철의 환경에서 사용자 평가 통해 효과적임을 검증하였다.본 연구는 교통약자와 비교통약자 두 그룹 간의 의사소통을 통해 임산부 승객에게 도움이 될 만한 서비스를 실제로 구현했다는 점에서 의의를 가진다. Among Mobility Handicapped, pregnant women are passengers who have difficulty using public transportation. The purpose of this study is to propose the design of a communicationservice for smooth communication between Mobility Handicapped and the regular passenger in public transport. For this purpose, we conducted a questionnaire and FGI for pregnant women in the pre-study. In Study 1, based on the results of the previous study, weproposed mobile-based communication services between the Mobility Handicapped and the regular passenger. Finally, in Study 3, user evaluation of services was carried out. As a result, we implemented and verified effective communication services between pregnant passengers and ordinary passengers, who are transportation users in public transportation by utilizing mobile apps.This study has significance in that it implemented services that would be useful to pregnant passengers through communication between the twogroups of the mobility Handicapped and the regular passenger.