Ralstonia eutropha의 유가식 발효에 의한 Poly(3-hydroxybutyrate) 생산의 경제성 분석

김갑진,양영기,이영하 한국미생물학회 2001 미생물학회지 Vol.37 No.1

High-cell-density cultivation of Ralstonia eutopha KHB 8862 by fed-batch fermentation in a 200 l pilot plant was carried out for the mass production of poly(3-hydroxybutyrate) (PHB). After 80 h of cultivation, the dry cell weight (DCW), PHB concentration, and PHB yield from fructose syrup reached 168 g/l, 74%DCW, and 0.27 (w/w), respectively, resulting in a productivity of 1.6 g of PHB/L/h. Based on these results, the PHB production cost from bacterial fermentation was analyzed and economic evaluation was performed. In the case of new investment being implemented or not, the production cost of PHB was US$ 3.15/kg and US$ 2.41/kg, respectively. PHB productivity and PHB yield on a carbon substrate were both important factors to be optimized. The increase of PHB yield on a carbon sources significantly decreased the PHB production cost but the increase in productivity had a relatively slight effect on the decrease in PHB production cost because the cost of carbon sources (37%) for PHB was larger in proportion to total cost than the depreciation cost (17%). These results suggest that the increased PHB yield from carbon sources and the development of new cheaper substrates would be more effective in decreasing PHB production cost than the increase in productivity. It was demonstrated that PHB is not in competition with consumable plastics such as PET in present market. Therefore, it is essential to lower production cost to be used as a bulk product and desirable to develop new application fields for PHB such as biomedical and cosmeceuticals. Pi1ot plant (200 l)에서의 유가식 배양에 의한 Ralstonia eutopha KHB 8862의 고농도 배 양을 통하여 Poly(3-hydroxybutyrate) (PHB)의 대량생산을 모색하였다. 그 결과 배양 80시간 후 168 g/l의 건체량과 건체량의 74%에 달하는 PHB를 생산할 수 있었으며, 이때의 고과당 시럽으로부터의 PHB 전환수율 및 PHB 생산성은 각각 0.27 (w/w) 및 1.6 $gl^{-1}$ $h^{-1}$ /이었다. 이를 토대로 본 연구에서는 미생물 발효에 의한 PHB 생산 cost 및 그 경제성을 분석하였다. 신규설비투자를 고려하지 않은 경우의 PHB의 생산 cost는 US$2.41/kg으로 산출된 반면에 신규 설비투자를 고려한 경우에는 US$3.15/kg으로 상승되었다. 탄소원의 PHB로의 전환수율과 발효 생산성 모두 PHB 생산비를 결정하는 중요요인이지만 전체 생산비의 37%를 차지하는 탄소원 원료비의 비중이 설비투자의 감가상각비 비중 (17%)에 비해 높기 때문에 생산성을 높이는 노력보다는 전환수율을 개선하는 것이 PHB 생산비용 절감의 핵심이 되는 것으로 나타났다. PHB chip으로의 제조시 PHB 생산 cost는 US$4.0/kg의 수준으로 현재로서는 범용 합성플라스틱에 비하여 경쟁력을 확보하지 못한다. 따라서 생산비 절감을 통한 범용수지로써 경쟁력 제고와 함께 바이오의약 분야 등의 고부가가치 영역에서의 새로운 용도 개발 등이 적극 요구된다.

Ralstonia eutropha KHB 8862의 유가배양에 의한 Pilot fermentor에서의 PHB 대량생산

김갑진,양영기,이영하 충남대학교 생물공학연구소 2004 생물공학연구지 Vol.10 No.2

A new PHB producer of Ralstonia eutropha KHB 8862 was developed and high cell density fed-batch fermentation was carried out to achieve the mass production of poly(3-hydroxybutyrate) (PHB), using firstly a lab scale and secondly a pilot scale 200L fermentor. During the fermentation process, fructose syrup was used as the sole carbon source and pH and nitrogen sources were controlled with NH₄OH solution. PHB accumulation was almost totally associated with nitrogen, oxygen and phosphate limitation during the fed-batch culture. We investigated the effect of initial volume on the cell growth, PHB accumulation and productivity in a 5 L jar fermentor and found that this small initial volume was very effective in increasing productivity. The importance of this work is that we could achieve, given an initial volume of 1.0 liter and a final volume of 3 L, a final cell concentration of 202 g/L, a PHB concentration of 156 g/L in 76hr, and hence a PHB productivity of 2.1 g/L/h. PHB content, cell yield from fructose syrup, and PHB yield from fructose syrup were 78%, 0.4, and 0.32 (w/w), respectively. Following these lab scale fermentation results, we scaled up the size to a pilot scale fermentor, using the same power input per unit volume (P/V=N3D5) and the same impeller tip speed (πND). A 200 L pilot fermentation run resulted in a final cell concentration of 168 g/L, with PHB content, PHB yield from fructose syrup and productivity being 74%, 0.27 (w/w) and 1.6 g/L/h, respectively.

다양한 모폴로지를 갖는 내충격성 개질 나일론 6-PPG 블록공중합체의 RIM 공정을 통한 합성과 물성에 관한 연구(I) -중합속도-

김갑진,조홍준,김영호 한국섬유공학회 1999 한국섬유공학회지 Vol.36 No.1

The copolymerization of $\varepsilon$-caprolactam(CL) and PPG-diamine has been performed using various activators such as tolylene dicarbamoyl dicaprolactam(TDC), hexamethylene dicarbamoyl dicaprolactam(HDC), and 1,3-bis(2-isocyanato-2-propyl) benzene/CL adduct(IDC) and sodium caprolactamate as a catalyst. The overall rate of polymerization of CL has been studied by fitting the experimental temperature rise with a polymerization kinetic equation involving the polymerization exotherm, polymerization-induced crystallization exotherm, and the heat loss due to nonideal adiabatic condition in the experimental situation. It was observed that for identical concentration of activator used in the presence of the impact modifier(PPG-diamine), HDC showed the fastest rate of polymerization and TDC showed the slowest one. The slowest activator TDC showed the highest yield of copolymerization, whereas IDC showed the lowest yield of copolymerization.

상수도수 불화사업 운영에 관한 평가분석

김갑진,이양규 대한상하수도학회 2003 상하수도학회지 Vol.17 No.4

The fluoride work management of water supply conveyance has been recommending by the WHO to prevent of tooth decay. Fluoridation of public water supplies has been practiced since 1945. The present approximately 67 countries reported community water fluoridation benetiting many cities. At our country, Fluoridation began in 1981 in Chongiu and Jinhea. In 2002, approximately 40 cities have large populations consuming fluoridated water. But Chongiu stopped fluoridation water works. Few public health measures have been accorded greater clinical and laboratory research, epidemiological study, clinical trials, and public attention than has water fluoridation. In this study, chemical analysis of Sodium Silicofluoride and Fluoride Feed Equipment analyzed. And this study proposed to Fluoride concentration experimental (lab. and field exp.), economics analysis, prevention effect. This study can be decided on the concentration of fluoride injection in Water Fluoridation. Hereafter, this study will be useful in safety and economics of Water Fluoridation in the future.

치과기공산업 발전 방안 및 추구해야 할 방안에 관한 인식도

김갑진 대한치과기공학회 2017 대한치과기공학회지 Vol.39 No.2

Purpose: In order to increase competitiveness for the growth and development of the dental laboratory industry, we plan to develop the dental laboratory industry. Methods: A total of 547 questionnaires were used as the final analysis data for the dental technicians from all over country participated in the 51st Korea Dental Technology Expo & Scientific Conference of the Korean Dental Technologist Association held in KINTEX from July 18 to 19, 2015. The questionnaire items consisted of 28 items in terms of general characteristics, questions about the methods to be pursued for the development of the dental laboratory industry, and recognition about the methods to be pursued to develop the dental laboratory industry. The collected data were analyzed by SPSS Ver. 21.0 for windows. Results: To improve the dental laboratory industry, actualization of dental laboratory products fee(41.2%) had the highest, followed by improving treatment of dental technician, standardization of dental laboratory products, direct bill of medical insurance, regulation of contract management on huge capital, and etc. The recognition of the measures to be pursued for the development of the dental laboratory industry was that dental laboratory products fee required to receive more than 20% of the dental prosthesis fee highest(4.62±0.76). And to enlarge dental laboratories through M&A between dental laboratories is the lowest(3.39±1.26). Conclusion: As a means to pursue the development of the dental laboratory industry, the actualization of dental laboratory products fee was proposed. Recognition also showed that dental laboratory products fee required to receive more than 20% of dental prosthesis fee was the highest. It is important to propose a reasonable dental laboratory products fee because it recognizes that it is necessary to promote economic growth in both development plan and awareness.

Vinylidene Fluoride and Trifluoroethylene (75/25) 공중합체의 상유전상에서의 새로운 결정전이

김갑진,이종순,조항원 한국섬유공학회 2002 한국섬유공학회지 Vol.39 No.3

When a vinylidene fluoride and trifluoroethylene (75/25) copolymer is crystallized isothermally from the melt at the undercooling of 12~16$^{\circ}C$, and then cooled using a hot stage having both thermal optical analysis and DSC functions on a polarizing microscope under cross polars, we can observe reproducibly a temperature range where the transmitted depolarized light intensity decreases abruptly with the evolution of heat upon cooling in the paraelectric state. Also in the course of reheating the melt-crystallized sample, we can observe an abrupt increase in the depolarized intensity just before melting and/or during melting. In this study, a newly found solid-state phase transition has been characterized. The transition seems to occur between the low birefringent paraelectric phase and the high birefringent paraelectric phase, showing a first order transition behavior like a Curie transition between the ferroelectric phase and the paraelectric phase. However, no significant chain-conformational change has been fecund in the new solid-state phase transition.

강자성 나노선에서의 줄열 발생 메커니즘 연구

김갑진,이재철,신경호,최선봉 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

광학현미경에 의한 섬유 및 필름의 굴절률 및 복굴절률 측정

김갑진 한국섬유공학회 1984 한국섬유공학회지 Vol.21 No.5

Poly(ethylene-2,6-naphthalate)의 고유물성의 측정 및 해석(II) -고유 물리적 상수-

김갑진,김상호 한국섬유공학회 2001 한국섬유공학회지 Vol.38 No.12

The intrinsic lateral sonic moduli of the crystalline and amorphous regions of PEN (ca. 6.161 and 2.357 GPa, respectively) were obtained from sonic moduli and crystallinities of unoriented poly(ethylene-2,6-naphthalate) (PEN) specimens which were annealed under different conditions. Using the obtained intrinsic lateral sonic moduli of the crystalline and amorphous regions, the degree of orientation of the amorphous region of uniaxially drawn PEN filaments could also be obtained from the crystallinity, sonic modulus, and the degree of crystalline orientation estimated from X-ray azimuthal scan data. Thus, the intrinsic birefringences of the perfectly oriented crystalline and amorphous regions of PEN, ca. 0.463 and 0.504, respectively, could be obtained from the combination of birefringence, crystallinity, and the degree of orientation of the crystalline and amorphous regions.

Wax-up으로 조각한 치관과 CAD로 설계한 치관의 형태 비교

김갑진 대한치과기공학회 2017 대한치과기공학회지 Vol.39 No.2

Purpose: With the development of digital dental technology, we are manufacturing prosthetic crown in various ways. However, the wax-up method that makes existing prosthetic crown is also used steadily. Thus, we will compare the shape of the crown designed with wax-up and the crown designed with CAD. Methods: Sculpt the crown with wax-up on the model to 10 dental technicians. The same model was used to design the crown CAD. Measure the size of the sculpted crown. The shapes were compared in three dimensions. Results: As a result, we could observe a difference in size and shape in crown designed with wax - up crown and crown. Conclusion: These results suggest that there are differences according to the method of carving crown and the method of processing.