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권오철,최은미,최형철,김용대,하정희,서장수,이광윤 대한기관식도과학회 1998 大韓氣管食道科學會誌 Vol.4 No.1
This study aimed at observing the effect of diazepam on the contractility of trachealis muscle isolated from canine trachea, possible involvement of central or peripheral type benzodiazepine receptor, and the calcium related mechanism of action of diazepam. Trachealis muscle strips of 15 mm long were suspended in an isolated organ bath containing 1 ml of physiologic salt solution maintained at $37^{\circ}C$, and aerated with 95% $O_2$ /5% $CO_2$. Isometric myography was performed. Diazepam reduced the basal tone concentration dependently, and this inhibitory action was not affected by neither flumazenil, a central benzodiazepine receptor antagonist, nor PK11195, a peripheral benzodiazepine receptor antagonist. Pretreatment with diazepam showed the inhibitory effect on the concentration-response curves to agonists such as bethanechol, 5-hydroxytryptamine and histamine. Diazepam also caused concentration-related inhibition of contraction with potassium chloride 30 mM. The effect of diazepam on the basal tone and potassium chloride-induced contraction with calcium channel blockers were compared. Similar results were obtained in canine trachealis with verapamil, nifedipine and diltiazem. These results suggest that diazepam relax an airway muscle not via specific receptors but by a similar action as calcium channel blockers in canine trachealis muscle.
권오철,최진영 대한의용생체공학회 1989 의공학회지 Vol.10 No.3
A new algorithm classifying ECG Arrhythmia signals using Back-propagation network is proposed. The base-line of ECG signal is detected by high pass filter and probability density function then input data are normalized for learning and classifying. In addition, ECG data are scanned to classify Arrhythmia signal which is hard to find R-wave. A two-layer perceptron with one hidden layer along with error back-propagation learning rule is utilized as an artificial neural network. The proposed algorithm shows outstanding performance under circumstances of amplitude variation, baseline wander and noise contamination.
영지버섯 미토콘드리아 SSU rDNA 부위의 SNP마커를 이용한 녹각형 영지버섯의 판별
권오철,박영진,김홍일,김종현,김지웅,이창수 한국버섯학회 2014 버섯 Vol.18 No.2
영지버섯은 두 가지 형태인 녹각형과 편각형이 알려져 있다. 최근 녹각형 영지버섯이 편각형 영지버섯보다 더 많은 양의 β-glucans 및 triterpenoids 가 함유되어있어 편각형 영지버섯보다 더 강한 생리활성이 있다고 제안되었으며, 실제로 녹각형 영지버섯의 추출물을 이용한 항종양활성 연구에서 종양억제의 탁월한 효과가 있다는 것이 보고되었다. 따라서 본 연구에서는 영지버섯 (Ganoderma lucidum) 종내 24균주(녹각형 5균주, 편각형 19균주)를 대상으로 미토콘드아 SSU rDNA의 염기서열을 비교 하였고 녹각형과 편각형 영지버섯을 판별할 수 있는 SNP 부위에 대해 분석을 수행하였다. 그 결과, SNP를 포함한 미토콘드리아 SSU rDNA 부위의 단편(499bp)을 증폭 하여 HinfI 제한효소를 처리하였을 때 녹각형 영지버섯에서만 369bp와 130bp 크기를 가진 두 개 의 제한 단편이 확인되었고 편각형 영지버섯에서는 499bp의 증폭산물이 유지되는 것을 확인하 였다. 이에 따라 녹각 형태의 영지버섯의 자실체 발생은 CO2 농도 및 광량뿐만 아니라 유전적인 관련성이 어느 정도 있을 것으로 사료된다.
권오철 한국지방행정연구원 1996 地方行政硏究 Vol.11 No.2
The conflicts between central and local governments in policy implementations have increased remarkably since local election june. 27. 1995. This paper offers suggestions for conflicts solution through officials' behaviors about the conflicts between central and local governments. The first part suggests the concept, the patterns, and the factors of the conflict, between central and local governments. The second part makes officials' behaviors analysis. And in the third part of this paper, five suggestions for conflicts solution are made as follows: 1. Conversing from negative to positive behavior 2. Making channels for conflicts solution