콜히친 침지처리에 의한 ‘망종화’의 4배체 식물유도

권수정,조갑연,김학현 한국자원식물학회 2013 한국자원식물학회지 Vol.26 No.2

This study aimed to get the basic data on the breeding of good varieties in Hypericum patulum Thunberg. Theoptimum materials, concentration and soaking time were examined to identify the effective approach to induce tetraploidplant by colchicine treatment to cultivate the varieties. For the seed germination rate of seed by colchicine treatment, thehigher colchicine concentration was and the longer soaking time was, the more the germination rate decreased. Whileindividuals were germinated in 16 test groups except control group (no treatment group), all the plants were diploid and notetraploid was induced. For the plant regeneration rate by colchicine treatment on the explant of Hypericum patulumThunberg that was under in vitro culture, the higher the colchicine concentration increased, the ress the regeneration rate. While total 147 individuals were regenerated in all treatment, when the explant was soaking treatment in more than 0.05%for over 6 hours, tetraploid could be obtained. In the soaking treatment of 0.05% for over 6 hours, tetraploid could beobtained. In particular, for the soaking treatment in 0.05% for 12 hours, 8 tetraploids were induced, which was about 47.1%of the number of plant regenerated. In accordance with the observation on doubling of DNA contents in leaf in order toidentify polyploidy, the peak DNA content of G1 phase was 94.5 for diploid and 192.5 for tetraploid. It confirmed doublingof DNA content. Furthermore, the number of chloroplasts per guard cell depending on polyploid was around 10 in diploidand 17 to 19 in tetraploid, which were around 1.7 to 1.9 times as much as diploid. ‘망종화’에서 콜히친 처리에 의한 효율적인 4배체 식물을 유도하고자 적정 식물체 부위, 적정농도 및 침지시간을검토하였다. 종자의 발아율은 콜히친의 농도가 높을수록또한 침지시간이 길수록 저하되었다. 대조구를 제외한 16개의 처리구에서 총 453개체가 발아된 반면 4배체는 유도되지 않았다. 기내 배양 중인 줄기 절편체의 식물체 재생율은 콜히친 처리농도 0.01%에서 최고를 나타내다가 0.1%농도 이상으로 높아질수록 낮았다. 적정식물체 부위는 줄기 절편체로 나타났다. 4배체 식물은 콜히친을 0.05% 이상으로 6시간 침지처리 하였을 때 얻을 수 있었고, 특히0.05%, 12시간 침지처리에서 식물체의 재생수 대비 약42%의 높은 획득율을 보였다. Flowcytometry에 의해DNA함량의 배가여부를 확인한 결과, G1 phase의 DNA 함량 peak가 2배체에서 94.5, 4배체는 192.5로, DNA가 배가됨을 확인할 수 있었다. 또한 공변세포 당 엽록체 수는2배체가 약 10개인 것에 비해 4배체는 17∼19개로 2배체보다 약 1.7∼1.9배 정도 많았다.

Effect of Colchicine on the Induction of Prunella vulgaris for. albiflora Nakai

권수정,ROY SWAPAN KUMAR,조갑연,문영자,우선희,김학현 한국작물학회 2015 한국작물학회지 Vol.60 No.1

This study was conducted to find out the effectiveinduction method of tetraploid plants to obtain potential datafor cultivating superior varieties by colchicine treatment. Theseed germination were decreased by the higher concentrationof colchicine treatment and longer soaking time. A total of907 individuals were germinated in 16 treated plots exceptcontrol (untreated plot) and 28 tetraploids were induced whichwas about 3.1% of the number of seed germinated. The plantregeneration rate by colchicine treatment on explant of Prunellavulgaris for. albiflora Nakai under in vitro culture was decreasedwith the higher concentration of colchicine. While a total of312 individuals were regenerated in all treatments, the explantwas soaked in more than 0.05% for over 1 hour, tetraploidcould be obtained. In particular, for the soaking treatmentin 0.05% for 6 hours and 12 hours, 37 tetraploids wereinduced, which was about 57.8% of the number of plantregenerated. In accordance with the observation on doublingof DNA contents in leaf in order to identify polyploid, thepeak DNA content of G1 phase was 101.3 for diploid and197.2 for tetraploid. The result confirmed the doubling ofDNA content. Furthermore, the number of chloroplasts per guardcell depending on polyploid was around 10 in diploid and19.3 in tetraploid, which was around 1.9 times as much asdiploid. Keywords : tetraploid, colchicine, DNA content, number ofchloroplasts, morphological characteristic

Effects of Ca-Gluconate on Fruit Firmness and Softening Enzyme Activities in Tomato using Hydroponics Systems

권수정,Guang-Jae Lee,Swapan Kumar Roy,Kab-Yeon Cho,Young-Ja Moon,조진웅,우선희,Hag-Hyun Kim 한국작물학회 2014 한국작물학회지 Vol.59 No.4

This study was carried out to investigate theeffects of Ca-gluconate (Ca-glu) on fruit firmness and softeningenzyme activities of hydroponically grown tomato (Solaniumesculentum Mill.). The obtained results revealed that therate of weight loss was markedly increased from at storageto 5 days after storage (DAS) in control, and was constantlyincreased until 7 DAS as 4.1% in Ca-glu treatment. Fruitfirmness was more rapidly decreased in Ca-glu induced fruitcompared to control. Results showed that fruit firmness incontrol and Ca-glu treated fruit were 0.67 and 0.95 kg·φ12mm-1, respectively. In our investigation, no difference wasrevealed in Hunter’s ‘a’ value between control and Ca-glutreated fruit. Total carotenoids content of control fruit wererapidly increased while the Ca-glu treated fruit were gentlyincreased. Lycopene content was higher (63.3 μg․g-1 FW) incontrol than Ca-glu treatment (56.8 μg․g-1 FW). The activityof Polygalacturonase (PG) was rapidly increased with increasingstorage period as from 0.4 to 1.2 units whereas the PG activityof Ca-glu treatment was gently increased from 1 to 7 DAS,and rapidly increased from 7 to 11 DAS. However, thepectinesterase (PE) activity was rapidly increased in controlfruit, when the storage period was increased, but interestingly,the Ca-glu treated fruit was slowly increased from 1 to 7DAS, and rapidly increased 7to 11 DAS. β-galactosidaseactivity of Ca-glu induced fruit was rapidly increased from1 to 7 DAS as from 1.6 to 3.0 units, and gently increasedfrom 7 to 11 DAS. β-galactosidase activity of control werehigher than Ca-glu treatment

Comparison of Growth Characteristics and Inorganic Components Between Korean and Japanese Codonopsis lanceolata

권수정,박태연,이문순,부희옥,조각연,우선희,조진웅,이희두,조성우,김학현 한국작물학회 2015 한국작물학회지 Vol.60 No.2

This study was performed to investigate the growth characteristics and inorganic components of Codonopsis lanceolata regarding regional differences. The plant height of Japanese Codonopsis lanceolata was 373.6 cm, so it’s revealed that it has more vigorous growth than Korean won. The flowering time of Korean Codonopsis lanceolata was 2 weeks faster than Japanese one. Total fresh weight of root was 41.0 g and 39.0 g for Korean and Japanese respectively, thus, no significance difference was found. However, regarding fresh weight, Korean one had a more fresh weight (35.4 g) of main root parts, but Japanese one had a more fresh weight (9.6 g) of the lateral root part. Each inorganic component was found more in the aboveground parts, regardless of the region and the content of K was the largest. Regarding the content of macroelements for each part of Codonopsis lanceolata, the content of Na, Mg, P, S, and Ca in Korean Codonopsis lanceolata was found the highest on the leaf, followed by stem and root. In the case of Japanese Codonopsis lanceolata, same result was found on the content of Mg and Ca, however, the highest content of Na and P was found in the stem.

Regional Comparison of Physiochemical Properties of Codonopsis lanceolata

권수정,박태연,이문순,부희옥,조각연,우선희,조진웅,이희두,조성우,김학현 한국작물학회 2015 한국작물학회지 Vol.60 No.2

This study was conducted to compare the growth, inorganic components, and proximate components of Codonopsis lanceolata grown in 10 regions of Korea for selecting superior species and breeding by crossing. Among the all tested lines, the shortest plant height (217.12 cm) was observed from the Ulleungdo region line (No. 4) while the longest (273.9 cm) was observed from Hwasun region line (No. 9). In addition, the lines of central and northern region (No. 1 ~No. 7) tend to have shorter plant height than those of southern region (No. 8~No. 9) except Jejudo region line (No. 10). Flowering tends to be late towards southern region, and lines in central and northern regions were started flowering about 2 weeks earlier than those in southern regions. However, the heaviest root weight was 13.1 g, found in only Jejudo line (No. 10) whereas there was no significant difference found in the other regions which have a range of 8.3~11.0 g. The inorganic components were varied in each line, however, proportion of macroelements, such as K, Ca, and P, was the largest for every line. Especially for Heongseong region line (No. 2), had larger proportion of macroelements than the others. There was a difference of proximate compositions of Codonopsis lanceolata, except the moisture content, among all regions, however, it was generally shown that the content of crude protein (1.31~3.76%) and crude fiber (2.18~3.12%) was the highest.

천명이 있는 영유아에서 뇨중 Leukotriene E4와 비인두액 Eosinophil Cationic Protein에 관한 연구

권수정,구본석,이하백,오재원 대한 소아알레르기 호흡기학회 2004 Allergy Asthma & Respiratory Disease Vol.14 No.1

‘녹색 꽃잎 도라지’의 기관분화에 미치는 배지조성 및 생 장조절제의 영향

권수정,조갑연,김학현 한국자원식물학회 2014 한국자원식물학회지 Vol.27 No.1

This study was carried out to determine the optimal medium composition and growth regulators for the micropropagation of Platycodon grandiflorum (Jacq.) A. DC. Nodes containing yellow green petals were used as plant materials to execute the study. The best performance of adventitious root development was found in 1/4 strength of MS basal salt and the growth was satisfactory in the concentration of 1/2 MS medium. The best condition for adventitious root development and growth was observed in the higher concentration (5%) of sucrose and activated charcoal free 1/4MS medium respectively. Adventitious roots were developed at the controlled culture medium at pH 4.8 with a tendency of suppression with higher levels of pH. However, it was prevailed that the development and growth depended on the concentration of agar. The lower concentration of agar (0.4%) was performed better than that of higher concentration (1.2%), whereas the agar concentration (0.4%) showed the best performance for the development and growth of adventitious roots. For the development of shoots containing node, BA combined with IAA was more effective than kinetin with IAA or NAA. The highest shoot development (3.9 shoots per explant) was performed on MS medium supplemented with 0.1 ㎎/L BA and 0.5 ㎎/L IAA. 본 연구는 ‘녹색 꽃잎 도라지’의 기내배양 시 배지구성물질의 적정농도 구명에 의한 대량번식을 목적으로 실시하였다. ‘녹색 꽃잎 도라지’의 절을 배양재료로 배양조건은 MS배지의 여러 가지 구성물질의 농도를 달리한 결과, 1/4MS 배지에서 가장 양호한 부정근의 형성을 보였으나 생장은 1/2MS배지에서 좋았다. Sucrose첨가는 농도가 높을수록 신초와 부정근의 형성 및 생장이 좋았다. 활성탄은 무첨가구에서 가장 많은 부정근의 형성과 양호한 생장을 보였다. 배지의 pH는 4.8로 조절된 배지에서 가장 많은 부정근을 형성하였으며, pH가 높아질수록 그 형성은 낮아지는 경향을 보였고, 부정근과 신초의 생장 또한 pH 4.8에서 가장 왕성하였다. Agar 농도별 실험에서 부정근의 형성과 생장은 그 농도가 낮아질수록 양호한 경향을 보여 가장 낮은 첨가구인 0.4% 농도구에서 가장 많은 부정근의 형성과 왕성한 생장을 보였다. 생장조절제를 혼용 첨가한 경우 신초의 형성은 BA와 IAA의 혼용구가 kinetin과 IAA 또는 NAA 혼용구에 비해 효과적이었으며, BA 0.1 ㎎/L와 IAA 0.5 ㎎/L 혼용구에서 절편체당 3.9개로 가장 많은 신초가 형성되었다.

민주노총, 봉하마을 꼭 가야했나

권수정 노동사회과학연구소 2009 정세와노동 Vol.- No.47

“스스로 요구하며 투쟁하는, 보다 많은 하청노동자 조직되어야” - 현대자동차아산공장사내하청지회

권수정 한국비정규노동센터 2004 비정규 노동 Vol.36 No.-

외국어로서의 한국어 문법 기술을 위한 연구 : 양보관계 연결어미를 중심으로

권수정 서울여자대학교 2011 태릉어문연구 Vol.17 No.-

본 연구는 실제 의사소통 상황에서 한국어 학습자가 다양한 양보관계 연결어미를 사용하지 못할 뿐더러 양보관계 연결어미를 사용 해야 하는 상황이 많음에도 불구하고 양보관계연결어미를 사용하지 못 하고 있는 문제의식에서 출발하였다. 따라서 본 연구에서는 많은 연결어미 중에서 양보관계 연결어미를 선정하여 한국어 교육을 위한 양보관계 연결어미에 대한 문법 기술을 시도하였다. 먼저 양보관계 연결어미의 문법 기술을 위하여 먼저 Celce-Murica & Lasen-Freeman(1999)가 제시한 문법 의 3차원에 대해서 살펴보았고 이를 통해 교사와 학습자를 위한 문법 기술은 형태·통사·의미·화용적 정보를 모두 포함해야 함을 알 수 있었다. 그래서 통사적 특성과 의미·화용적 특성을 검토하여 통사·의미·화용 적 정보를 마련하였다. 그리고 이 결과를 바탕으로 교사와 한국어 학습자에게 제시되어야 할 문법 항목, 즉 형태·통사·의미·화용 적 정보가 포함된 문법 틀을 마련하였다.