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두충(Eucommia ulmoides O.)잎으로부터 Grb2-Shc 결합저해 활성물질의 분리
한재택,안은미,방면호,남지연,권병목,백남인,Hahn, Jae-Taek,Ahn, Eun-Mi,Bang, Myun-Ho,Nam, Ji-Youn,Kwon, Byoung-Mog,Baek, Nam-In 한국생약학회 1999 생약학회지 Vol.30 No.2
The MeOH extracts obtained from the leaves of Eucommia ulmoides were solvent-fractionated with EtOAc, n-BuOH, and $H_20$, successively. From the EtOAc extract showing Grab2-Shc inhibitory activity, a flavonol and a lignan compounds were isolated through repeated silica gel column chromatographies. By interpretation of several spectral data and adaptation of acetylation method, the chemical structures of the compounds were determined as 5,7,3',4'-tetrahydroxy-flavonol (quercetin), and 4,4',8',9-tetrahydroxy-3,3'-dimethoxy-7,9'-cyclolignan {(-)-olivil)}, respectively. The compounds exhibited $IC_{50}$ values in Grb2-Shc inhibitory activity to be 93 and $210\;{\mu}mole/l$, respectively.
홍화 ( Carthamus tinctorius L. ) 로부터 활성물질의 분리
백남인,김융희,안은미,방면호,남지연,권병목 ( Nam In Baek,Yung Hee Kim,Eun Mi Ahn,Myun Ho Bang,Ji Youn Nam,Byung Mok Kwon ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.2
The MeOH extracts obtained from the flower petals of Carthamus tinctorius were solvent-fractionated with EtOAc, n-BuOH, and H₂O, successively. From the n-BuOH exact 2 flavonoid compounds were isolated through the repeated silica gel column chromatographies. From not only the results of physico-chemical data including HMBC but also the adaptation of acid hydrolysis, the chemical structures of the compounds were determined as 3-Ο-[β-D-glucopyranosyl (1→2) β-D-glucopyranosyl] kaempferol and 3-Ο-[α-L-rhamnopyranosyl (1→6) β-D-glucopyranosyl] kaempferol. The compounds exhibited IC_(50) values in Grab2-Shc activity to be 43 and 47 ㎍/㎖, respectively.
식용식물자원으로부터 활성물질의 탐색-XVII. 산딸나무(Cornus kousa Burg.)의 열매로부터 sterol 화합물의 분리
이대영,송명종,유종수,김성훈,정인식,김대근,박미현,권병목,김세영,백남인,Lee, Dae-Young,Song, Myoung-Chong,Yoo, Jong-Su,Kim, Sung-Hoon,Chung, In-Sik,Kim, Dae-Keun,Park, Mi-Hyun,Kwon, Byoung-Mog,Kim, Se-Young,Baek, Nam-In 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.1
산딸나무 열매를 100% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복하여 3종의sterol 화합물을 분리, 정제하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, ${\beta}-sitosterol$(1), $stigmast-4-en-6{\beta}-ol-3-one$(2), daucosterol(3)으로 동정하였다. 이 화합물들은 산딸나무에서 처음 분리되었다. The fruits of Cornus kousa Burg. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc fraction, three sterols were isolated through the repeated silica gel and ODS column chromatographies. According to the results of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as ${\beta}-sitosterol$ (1), $stigmast-4-en-6{\beta}-ol-3-one$ (2) and daucosterol (3). They were the first to be isolated from Cornus kousa Burg.
식용 식물자원으로부터 활성물질의 탐색 -XIII. 개망초(Erigeron annuus L.) 꽃으로부터 triterpenoid의 분리-
김동현,정성제,방명호,정인식,김성훈,권병목,김대근,박미현,백남인,Kim, Dong-Hyun,Jung, Sung-Je,Bang, Myun-Ho,Chung, In-Sik,Kim, Sung-Hoon,Kwon, Byoung-Mog,Kim, Dae-Keun,Park, Mi-Hyun,Baek, Nam-In 한국응용생명화학회 2004 Applied Biological Chemistry (Appl Biol Chem) Vol.47 No.4
개망초의 꽃을 80% MeOH 용액으로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 EtOAc 분획을 silica gel, octadecylsilica gel(ODS) column chromatography 및 high performance liquid chromatography(HPLC)로 정제하여 3종의 화합물을 분리하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, ${\alpha}-amyrenone(1),\;{\alpha}-amyrin(2)$ 및 ${\beta}-amyrin(3)$으로 동정하였다. 이들 3종의 triterpenoid는 개망초에서는 처음 분리되었다. The flower of Erigeron annuus L. was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc fraction, three compounds were isolated through the repeated silica gel, ODS column and high performance liquid chromatographies. From the result of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as ${\alpha}-amyrenone,\;{\alpha}-amyrin\;and\;{\beta}-amyrin$. These three compounds were isolated for the first time from the flower of Erigeron annuus L.
쑥갓세포의 현탁배양에 의한 ${\beta}-sitosterol$ 생산
김현철,정하영,이소연,정호용,김유정,백남인,김성훈,최근원,김대근,권병목,박미현,정인식,Kim, Hyun-Chul,Chung, Ha-Young,Lee, So-Youn,Chung, Ho-Yong,Kim, You-Jung,Baek, Nam-In,Kim, Soung-Hoon,Choi, Geun-Won,Kim, Dae-Keun,Kwon, Byoung-Mok,Park 한국응용생명화학회 2005 Applied Biological Chemistry (Appl Biol Chem) Vol.48 No.4
[${\beta}-sitosterol$]은 식물 스테롤로서 인간의 전립선암과 대장암 세포의 성장을 억제하고 생체내 콜레스테롤 농도를 감소시킨다. 본 연구에서는 쑥갓세포 배양에서 ${\beta}-sitosterol$ 생산의 최적화 연구를 수행하였다. 그래서 쑥갓(Chrysanthemum coronarium L.)으로부터 캘러스 유도는 NAA와 BAP의 농도가 각각 1 mg/l의 조합에서 최적이었으며 이들 캘러스로부터 현탁배양 세포주를 확립하였다. 현탁 배양시 초기 세포농도 2 g DCW/l에서 조성이 각각 1배인 탄소원(30 mg/l), 질소원(1900 mg/l $KNO_3$, 1650mg/l $NH_4NO_3$), 무기인산원(170 mg/l)을 포함하는 MS 배지에서 ${\beta}-sitosterol$ 생산이 최적으로 나타났다. Shake-flask를 이용한 현탁배양에서 ${\beta}-sitosterol$의 최대 생산량은 $150{\mu}g/g$ DCW이었다. 그리고 공기부유식 생물반응기의 배양에서는 100 cc/ml의 통기량에서 ${\beta}-sitosterol$의 생산이 $142.8{\mu}g/g$ DCW으로 나타났다. [${\beta}-sitosterol$] is a plant sterol that reduces cholesterol levels and inhibits the growth of human prostate and colon cancer cells. Optimal conditions for ${\beta}-sitosterol$ production were examined from cell suspension cultures of Chrysanthemum coronarium L. The callus induction was optimal in MS medium containing 1 mg/l NAA and 1 mg/l BAP. Cell suspension culture was also established from the callus. Optimal ${\beta}-sitosterol$ production was obtained when the cells were cultured at an initial density of 2 mg DCW/l in MS medium containing 1 X sucrose (30 mg/l), 1 X nitrogen (1900 mg/l $KNO_3$, 1650 mg/l $NH_4NO_3$), and 1 X phosphate source (170 mg/l). In cell suspension cultures of C. coronarium L. using shake flasks, the peak content of ${\beta}-sitosterol$ was $150{\mu}g/g$ DCW. In cell suspension cultures of C. coronarium L. using an air-lift bioreactor, the maximum ${\beta}-sitosterol$ content of $143.8{\mu}g/g$ DCW was obtained at an air-flow rate of 100 cc/min.
$2{\beta}$, $3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid처리에 의한 인간 간암세포주 HepG2의 apoptosis 유도
유기현,이종민,황보전,송명종,양혜정,백남인,김성훈,김대근,권병목,박미현,정인식,Yoo, Ki-Hyun,Lee, Jong-Min,HwangBo, Jeon,Song, Myoung-Chong,Yang, Hye-Joung,Baek, Nam-In,Kim, Soung-Hoon,Kim, Dae-Keun,Kwon, Byoung-Mok,Park, Mi-Hyun,Chung, 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.4
Triterpenoid를 포함하고 있는 $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid를 애기마름으로부터 분리하였다. 이것은 pentacyclic triterpenes의 공통 구조를 가지며 amyrin ursolic acid 그룹에 속해 있다. 본 연구에서는 이 화합물의 독성 영향을 인간 간암 세포주인 HepG2에서 조사하였다. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid는 처리한 양에 비례하여 HepG2 세포주에서 독성을 보였다. 그리고 Confocal microscopy 결과는 $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid를 HepG2 세포에 처리한 시간에 비례하여 녹색 형광의 증가를 보여주었다. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid는 또한 HepG2 세포의 sub-G1 cell population 뿐만 아니라 DNA 분절(fragmentation) 현상의 증가를 보여 주었다. 이러한 결과는 $22{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid가 HepG2 세포에서 apoptosis를 통한 세포 사멸 유도를 의미한다. [ $2{\beta},\;3{\alpha}$ ], 23-trihydroxyrus-12-ene-28-oic acid was isolated from Trapa pseudoincisa S. et Z. It has a common structure of pentacyclic triterpenes and belongs to the amyrin ursolic acid group. The cytotoxic effect of this compound was investigated in human hepatoma cell line HepG2. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid showed dose-dependent cytotoxicity in HepG2 cells. Confocal microscopy data showed that green fluorescence was increased in $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid treated-HepG2 cells in a time-dependent manner. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid also increased the sub-G1 cell population of HepG2 cells as well as ladder-like DNA fragmentation. Taken together, our results indicate that $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid induced apoptosis in HepG2 cells.
미생물,발효,생물공학 : 2β, 3α, 23-trihydroxyrus-12-ene-28-oic acid 처리에 의한 인간 간암세포주 HepG2의 apoptosis 유도
유기현 ( Ki Hyun Yoo ),이종민 ( Jong Min Lee ),황보전 ( Jeon Hwangbo ),송명종 ( Myoung Chong Song ),양혜정 ( Hye Joung Yang ),백남인 ( Nam In Baek ),김성훈 ( Soung Hoon Kim ),김대근 ( Dae Keun Kim ),권병목 ( Byoung Mok Kwon ),박 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.4
쑥갓세포의 현탁배양에 의한 β-sitosterol 생산
김현철 ( Hyun Chul Kim ),정하영 ( Ha Young Chung ),이소연 ( So Youn Lee ),정호용 ( Ho Yong Chung ),김유정 ( You Jung Kim ),백남인 ( Nam In Baek ),김성훈 ( Soung Hoon Kim ),최근원 ( Geun Won Choi ),김대근 ( Dae Keun Kim ),권병목 ( 한국응용생명화학회 2005 Applied Biological Chemistry (Appl Biol Chem) Vol.48 No.4