cis-Diamminedichloroplatinum (II) induces denaturation and conformational changes in pBR322 DNA

구자춘,임창수,한태룡,양재명,Koo, Ja-Choon,Lim, Chang-Soo,Hahn, Tae-Ryong,Yang, Jai-Myung 한국응용생명화학회 1990 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.4

CDDP를 처리한 pBR322 DNA로 형질변환된 대장균 LE392를 ampicillin이 포함된 한천평판배지위에 도말시켰다. Ampicillin을 함유하고 있는 평판배지위에 형성된 집락수는 13.3 ${\mu}M$의 CDDP를 처리한 뒤에는 검출되지 않을 정도로 감소하였다. CDDP를 처리한 pBR322 DNA는 외가닥 DNA에 특이성이 있는 S1 핵산분해호소에 의해 전달되었고 아가로즈 겔 전기영동상에서 이동 유형이 변했다. 이러한 결과에 의하면 CDDP가 pBR322 DNA에 반응하여 이증나선의 변성과 궁극적으로는 ampicillin 저항성 유전자를 불활성화시키는 구조변화를 일으키는 것 같다. E. coli LE392, transformed with CDDP-treated pBR322 DNA, was plated on ampicillin containing media. The number of colonies formed on ampicillin containing agar plate was reduced to undetectable level after treat the DNA with 13.3 ${\mu}M$ CDDP. The CDDP-treated pBR322 DNA was susceptible to sing1e strand DNA specific S1 nuclease and it's migration Pattern in agarose gel electrophoresis was changed. These results suggest that CDDP adduction to pBR322 DNA resulted in denaturation of the double helix and changes in it's conformation which ultimately leads In the inactivation of the ampicillin resistant sere.

최대 엔트로피 기법으로 도출한 지속 가능한 송이 생산 전략

구자춘 ( Ja Choon Koo ),최준영 ( Jun Yeong Choi ),윤여창 ( Yeo Chang Youn ) 한국산림과학회 2013 한국산림과학회지 Vol.102 No.3

Pine mushroom (Tricholoma matsutake) is one of the most profitable forest products in Korea. We postulated a hypothesis that a high rate of returns to labor input could make the harvest of pine mushroom off the optimum level. In the view of developing a sustainable production strategy for pine mushroom producers,production of pine mushroom collectors and pine mushroom growth function were estimated using maximum entropy method. Annual pine mushroom production and labor input were the data used in the estimation of production function of pine mushroom collectors and pine mushroom growth function. The level of sustainable maximum production derived from the estimated function. The production function estimated shows that production of pine mushroom is affected more by the resource of pine mushroom stocked in the forests than by labor that households put in forestry business. The production function of mushroom collectors and the estimated growth function indicate that pine mushroom harvests for the period of 2005-2011 did not reach the potential level of maximum sustainable production. Therefore, we suggest that pine mushroom harvest should be controlled until the resource stock of pine mushroom in the forests increases to the level of maximum sustainable production.

애기장대 APETALA3의 유전인자형 결정

구자춘 ( Ja Choon Koo ),이은선 ( Eun Sun Lee ),송경자 ( Kyoung Ja Song ),( Krishnamoorthy Srikanth ),황성수 ( Sung Soo Whang ) 전북대학교 과학교육연구소 2011 과학과 과학교육 논문지 Vol.36 No.-

The ABC model describes the development of floral organs in plants. In this study the pattern of floral organ development was evaluated by artificial crossing of wild type Arabidopsis with mutant Arabidopsis plants and then their phenotype and genetic characters were investigated. Wild type Arabidopsis plant consist of 4 sepal, 4 petal, 6 stamen and 2 carpel but in ap3 mutant a gene of B function, the petals are converted into sepal and stamen is converted into pistil. This indicated a strong recessive homozygous mutant phenotype. Based on PCR method, genotyping of both wild and mutant type were determined in F1 and F2 generation respectively.

산주와 임업인의 인식 분석을 통한 산림환경서비스 지불제의 도입 가능성 평가

구자춘 ( Ja Choon Koo ),정호근 ( Ho Gun Chong ),석현덕 ( Hyun Doek Seok ) 한국임학회 2014 산림과학 공동학술대회 논문집 Vol.2014 No.-

본 논문은 일반국민과 임업인의 산림환경서비스 지불제 도입에 관한 의사를 분석하여 제도의 도입 가능성을 평가하고, 도입 가능성을 높이기 위한 정책 방향을 제안하기 위하여 마련되었다. 2012년 일반국민 1,033명과 임업인 500명을 대상으로 실시한 설문조사에서 일반국민 40.3%와 임업인34.4%가 산림환경서비스 지불제 도입에 찬성하였다. 다항로짓모형으로 분석한 결과, 나이, 학력, 소득, 산림정책에 대한 평가, 산지 소유 여부가 지불제에 대한 의사에 유의한 영향을 미치는 것으로 나타났다. 산림환경서비스 공급자는 지불제 시행에 부정적 입장을 취하였고, 정부 주도의 산림정책 추진이 지불제 찬성에 긍정적인 영향을 미치는 것으로 나타났다. 따라서 지불제의 원활한 도입을 위해 서는 정부가 공급자의 채권을 보장해줄 정도의 강한 리더십을 발휘해야 할 것이다. This study aims to evaluate contingent feasibility of introducing payment for forest ecosystem services(PES) system by analyzing an intention of general public and foresters. 1,033 general public and 500 foresters were surveyed in 2012. 40.3% of general public and 34.3% of forester agreed to PES system, incentives offered to forest owners in exchange for managing their forest to provide forest ecosystem services. The Results of multinomial logit model show that age, income, education, attitudes toward current forest policies and ownership have a significant effect on their intention. Specifically, forest owners, suppliers of services show a negative view of introducing PES system. And government-led forest policy has a positive effect on respondent``s willingness to agree to PES. Thus, in order to facilitate PES system, Korean government should demonstrate strong leadership enough to ensure the bond of supplier.

기업의 환경경영 참여에 영향을 미치는 선행요인에 관한 연구

구자춘(Ja-Choon Koo),윤여창(Yeo-Chang Youn),조동성(Dong-Sung Cho),노태우(Tae-Woo Roh) 한국전략경영학회 2013 전략경영연구 Vol.16 No.1

본 연구는 기업이 처한 외부적 환경변화와 내부적 요인인 환경경영 참여에 어떠한 영향을 미쳤는지에 대한 답을 제시하기 위해 마련되었다. 구체적으로 본 연구는 기후변화시대에 온실가스 상쇄수단으로 부상한 산림 탄소배출권에 대한 구매 의사를 기업이 환경경영에 참여하는 것으로 간주하였고, 이에 기업의 주관적 규범, 행동태도, 지각된 행동제어요소가 제도적 동형화 과정의 종류에 따라 어떠한 영향을 미쳤는지를 규명한 것이다. 본 연구의 틀은 계획행동이론에 기반을 두었으며, 가설은 제도적 동형화 이론에 근거하여 설정되었다. 2011년 매출액 기준 상위 200대 기업을 대상으로 면대면 설문조사를 시행한 뒤, 70개 기업으로부터 회수된 자료를 이용하여 Heckman의 2단계 검증을 이용한 이항로짓모형을 활용하여 가설 검증을 시도하였다. 연구 결과, 환경관련 사업경험이 있고, 비용절감을 위해서 감축계획을 수립한 기업일수록 산림 탄소배출권을 구매할 확률이 높았으며, 위 두상황이 주어졌을 때 목표관리제에 포함된 기업일수록 산림 탄소배출권 구매확률이 높은 것을 발견하였다. In this study, we postulate firms are willing to purchase forest carbon offsets as the firm practices environmental management. Even though a firm is usually controlled by stakeholder, it would not invest in the cost, unless it is associated with their current interests. Thus, the behavior of purchasing forest carbon credits that is not directly related with current interests can be regarded as an active participation in environmental management. Through literature reviews, the research question is drawn: “which factors make firms more likely to take part in environmental management under institutional pressures?” To address this question, the theory of planned-behavior and institutional isomorphism were employed. Hypotheses are examined by logit and Heckman’s two-step estimation and results show that a firm that is experienced environment-related projects and set the energy reduction plan is more likely to purchase forest carbon offsets.

해밀톤역학을 이용한 충격현상의 모델링

구자춘(Ja Choon Koo) 한국소음진동공학회 2001 한국소음진동공학회 학술대회논문집 Vol.2001 No.11

빅데이터 분석을 활용한 제5차 산림기본계획 주요 사업에 대한 평가

변승연,구자춘,석현덕,Byun, Seung-Yeon,Koo, Ja-Choon,Seok, Hyun-Deok 한국산림과학회 2017 한국산림과학회지 Vol.106 No.3

In This study, we examined the gap between supply and demand of forest policy by year through big data analysis for macroscopic evaluation of the 5th Basic Forest Plan. We collected unstructured data based on keywords related to the projects mentioned in the news, SNS and so on in the relevant year for the policy demand side; and based on the documents published by the Korea Forest Service for the policy supply side. based on the collected data, we specified the network structure through the social network analysis technique, and identified the gap between supply and demand of the Korea Forest Service's policies by comparing the network of the demand side and that of the supply side. The results of big data analysis indicated that the network of the supply side is less radial than that of the demand side, implying that various keywords other than forest could considerably influence on the network. Also we compared the trends of supply and demand for 33 keywords related to 27 major projects. The results showed that 7 keywords shows increasing demand but decreasing supply: sustainable, forest management, forest biota, forest protection, forest disease and pest, urban forest, and North Korea. Since the supply-demand gap is confirmed for the 7 keywords, it is necessary to strengthen the forest policy regarding the 7 keywords in the 6th Basic Plan.

북한 산림전용 방지수단으로서의 REDD 사업의 경제적 타당성 분석

조장환 ( Jang Hwan Jo ),구자춘 ( Ja Choon Koo ),윤여창 ( Yeo Chang Youn ) 한국산림과학회 2011 한국산림과학회지 Vol.100 No.4

This study aims to verify the economic validity of the REDD project in North Korea by estimating the potential carbon credits and the cost of REDD project. The REDD potential credits of North Korea are estimated based on the international statistics of forest area and population from 1990 to 2010, and the cost of REDD project is estimated indirectly by annual land opportunity cost of agriculture assuming that South Korea will aid the food production per area in North Korea. When the 25% reduction scenario was applied to the annual deforestation rate in North Korea, the potential REDD credits were estimated to be 4,232 million~5,290 milliontCO2eq. for 20 years. It would account for 28~35% of South Korea`s national medium-term greenhouse gas reduction target. On the other hand, the break-even price of REDD project was calculated as the profit of agriculture in the land available by forest conversion in North Korea. It was estimated to be 19.19$/tCO2eq. when the non-permanence risk of forest conserved through a REDD contract is assumed to be 20%. This price is higher than the price of REDD carbon credit 5$/tCO2eq. dealt in the 2010 voluntary carbon market, leading to no economic feasibility. However, REDD project provides co-benefits besides climate mitigation. As previous studies indicate, the break-even price is lower than 20$/tCO2eq., which is the social marginal cost of greenhouse gas emissions by loss of forest. Therefore REDD in North Korea can be justified against the social benefits. The economic feasibility of REDD project in North Korea can be largely influenced by the risk percentage. Thus, North Korean REDD project needs a strong guarantee and involvement by the government and people of North Korea to assure the project`s economic feasibility.

능동 컴플라이언스를 활용한 나사 체결 및 해체 연구

박진석(Jinseok Park),구자춘(Ja Choon Koo) 대한기계학회 2024 대한기계학회 춘추학술대회 Vol.2024 No.4

인간 조립작업 정량화 분석을 통한 로봇 커넥터 조립 최적화 방안 연구

유영재(Young Jae You),구자춘(Ja Choon Koo) 대한기계학회 2024 대한기계학회 춘추학술대회 Vol.2024 No.4