http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Response of Bioluminescent Bacteria to Sixteen Azo Dyes
구만복,Hwa Young Lee,Sue Hyung Choi 한국생물공학회 2003 Biotechnology and Bioprocess Engineering Vol.8 No.2
Recombinant bioluminescent bacteria were used to monitor and classify the toxicity of azo dyes. Two constitutive bioluminescent bacteria, Photobacterium phosphoreum and Escherichia coli, E. coli GC2 (lac::luxCDABE), were used to detect the cellular toxicity of the azo dyes. In addition, four stress-inducible bioluminescent E. coli; DPD2794 (recA::luxCDABE), a DNA damage sensitive strain; DPD2540 (fabA::luxCDABE), a membrane damage sensitive strain; DPD2511 (katG::luxCDABE), an oxidative damage sensitive strain; and TV1061 (grpE::luxCDABE), a protein damage sensitive strain, were used to provide information about the type of toxicity caused by crystal violet, the most toxic dye of the 16 azo dyes tested. These results suggest that azo dyes result in serious cellular toxicity in bacteria, and that toxicity monitoring and classification of some azo dyes, in the field, may be possible using these recombinant bioluminescent bacteria.
Specific Detection of DNA Using Quantum Dots and Magnetic Beads for Large Volume Samples
구만복,김연석,Byoung Chan Kim,Jin Hyung Lee,Jungbae Kim 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.5
Here we present a sensitive DNA detection protocol using quantum dots (QDs) and magnetic beads (MBs) for large volume samples. In this study, QDs, conjugated with streptavidin, were used to produce fluorescent signals while magnetic beads (MBs) were used to isolate and concentrate the signals. The presence of target DNAs leads to the sandwich hybridization between the functionalized QDs, the target DNAs and the MBs. In fact, the QDs-MBs complex, which is bound using the target DNA, can be isolated and then concentrated. The binding of the QDs to the surface of the MBs was confirmed by confocal microscopy and Cd elemental analysis. It was found that the fluorescent intensity was proportional to concentration of the target DNA, while the presence of non-complementary DNA produced no significant fluorescent signal. In addition, the presence of low copies of target DNAs such as 0.5 pM in large volume samples up to 40 mL was successfully detected by using a magnet-assisted concentration protocol which consequently results in the enhancement of the sensitivity more than 100-fold.
Characterization of gltA::luxCDABE Fusion in Escherichia coli as a Toxicity Biosensor
구만복,안주명,Byoung Chan Kim 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.6
The use of gltA gene, as a new biomarker for environmental stress biomonitoring, was investigated because of its key position as the first enzyme of the tricarboxylic acid (TCA) cycle. A recombinant bioluminescent Escherichia coli strain, EBJM2, was constructed using a plasmid carrying the citrate synthase (gltA) promoter transcribing the Photorhabdus luminescens luxCDABE genes (gltA::luxCDABE). The responses from this strain were studied with five different classes of toxicants: DNA damage chemicals, phenolics, oxidative-stress chemicals, PAHs, and organic solvents. EBJM2 responded strongly to DNA damage chemicals, such as mitomycin C (MMC) and methyl-nitro-nitrosoguanidine (MNNG) and nalidixic acid with the strongest responses. In contrast, tests with several compounds from the other four classes of toxicants gave no significant response. Therefore, EBJM2 was found to be sensitive to DNA damage chemicals.
경도인지장애(MCI)노인의 인지기능 증진을 위한 인지기반 중재에 관한 연구
조만복 ( Jo Man-bok ) 한국노인의료복지학회 2017 노인의료복지연구 Vol.9 No.1
The purpose of this study is to identify the trends of recent studies on cognitive-based intervention for the improvement of cognitive function of the elderly with mild cognitive impairment(MCI). Recent studies on cognitive-based intervention for cognitive enhancement have shown that cognitive-based interventions and programs for the improvement of cognitive function of the elderly with mild cognitive impairment have been improving in various areas ranging from objective cognitive abilities such as memory, language abilities, and time and space abilities to subjective self-assessments such as quality of life, self-efficacy, and depression. Based on the evidences that functional reorganization of the brain can enhance brain function in the aging process and that there is a positive change in the attributes, it should be accompanied by not only a study on medication method but also a non-medication method as a way to strengthen the cognitive function of the elderly.