수온과 광주기에 의한 볼락(Sebastes inermis)의 번식주기 조절

고창순,장영진,임한규,김종현,조기채,KO Chang Soon,CHANG Young Jin,LIM Han Kyu,KIM Jong-Hyun,CHO Kee Chae 한국수산과학회 1998 한국수산과학회지 Vol.31 No.5

유용 증양식 대상종으로 새끼를 낳는 볼락을 재료로하여 수온과 광주기 조절이 성숙과 번식주기에 미치는 효과를 파악하고, 출산시기의 제어 가능성을 검토하였다. 월별 GSI의 변화에서 암컷은 Exp.II와 III에서는 서로 큰 차이없이 11월부터 증가하기 시작하여 1월에 각각 13.0과 14.7로 최고값이 되었다. 수온과 광주기를 각각 2 개월씩 늦춘 Exp.I에서 조절에 반응하였던 어미들은 Exp.II와 III에 비해 1개월 늦어진 2월에 15.0으로 최고 값을 나타냈다. 그러나 Exp.I에서 조절에 반응하지 않았던 어미들은 전 실험기간 동안 0.5 이하로 낮게 유지되다. 수컷 GSI의 월별변화는 실험구에서 9월부터 증가하기 시작하여 Exp.I에서는 10월, Exp.II와 III에서는 11월에 각각 최고값을 보인후 급격히 감소하였으며, 실험구별로 차이를 나타내지 않았다. HSI의 월별 변화는 GSI와는 서로 상반된 경향을 나타냈으며, 수온과 광주기의 조절은 실험구별 어체의 GSI와 비만도의 변화에 영향을 미치지 않았다. 실험구별 생식소의 발달단계를 관찰한 결과, Exp.I에서 수온과 광주기 조절에 반응하였던 개체들의 난소는 Exp.II와 III에 비해 성숙과 출산이 지연되는 조직상을 나타냈으나, 정소에서는 실험구별 발달단계의 차이를 인정할 수 없었다. 수온과 광주기를 자연상태로 유지한 것과 이를 2개월 늦춘 실험구를 설정하여 사육한 결과, 암컷 볼락은 대조구 (Exp.II와 III)에 비해 조절구 (Exp.I-r)에서 성숙과 출산이 1$\~$2개월 지연된 반면, 수컷에서는 수온과 광주기의 조절 효과가 인정되지 알았다. Experiments on the control of rockfish, Sebastes inermis reproduction were performed by artificially changed water temperature (WT) and photoperiod (PP). The experiments were divided into three conditions; artificial changes of both WT and PP delayed two months against their natural variations (Exp.I), natural WT and PP replaced with artificial light (Exp.II) and natural WT and PP (Exp.III) from September to April (220 days). Initial gonadosomatic index (GSI) in female groups of experiments was 0.2. Female GSI of responded group in Exp.I began to increase from December and reached the highest value in February with 15.0, while the un-responded group kept the lower value during the experimental period. Female GSI of Exp.II and Exp.III reached the highest values in January with 13.0 and 14.7, respectively. Male GSI of Exp,I reached the maximum in October, and that of Exp.II and III reached the maximum in November and then rapidly decreased. Among the experimental groups, male GSI did not show the different tendency. Monthly changes of hepatosomatic index in both sexes showed the reciprocal tendency with those of GSI. The control of WT and PP did not affect on the changes of HSI and condition factor of rockfish. From the histological observations, maturation of ovary was delayed by the changes of WT and PP, but that of testis was not influenced. Judging from the above results, the maturation and parturition of female rockfish could be delayed one or two months by artificial changes of WT and PP.

주사용(走査用) $^{113m}In$ 교질(膠質)의 조제(調製) 및 흰쥐에서의 장기분포(臟器分布)에 관(關)한 연구(硏究)

고창순,이종헌,장고창,홍창기,Koh, Chang-Soon,Rhee, Chong-Heon,Chang, Ko-Chang,Hong, Chang-Gi D. 대한핵의학회 1969 핵의학 분자영상 Vol.3 No.1

The newly developed diagnostic method with application of $^{113}Sn-^{113m}In$ cow system ($^{113}Sn:\;T\frac{1}{2}$ 118 days, $^{113m}In:\;T\frac{1}{2}$ 1.7 hrs, 390 Kev, Single ${\gamma}$) has the remarkable advantages such as increased diagnostic ability by single large dose administration of $^{113m}In$ with no subsequent radiation hazard and shortened examining time. We reformed the research of following scope with the use of developed $^{113}Sn-^{113m}In$ cow (25 mCi) generator: The sizes of particles produced under various conditions were investigated, and possibility for application to the scannings of various organs such as brain, liver, lung, bone marrow and blood pool etc. were studied. Results: $^{113m}InCl_3$ solution eluted from diluted HCl solution (pH 1.5) passed through $^{113}Sn-^{113m}In$ generator, and there can be produced various sized particles of colloidal indium. And there observed the state of distribution of $^{113m}In$ in each organ which showed many differences according to the particle sizes of colloidal indium. The results are stated as follows: 1. The adjustment of pH is the most important factor in making the desirable particle size of colloidal indium. The colloid for blood pool showed the highest level as 7.1%/gm blood, at pH 1.7, the colloid of pH 3.5 for liver scanning showed the highest level, 88.4%, in the liver, the colloid pH 6 showed the highest level, 3.1%, in the spleen, and the colloid of pH 11.0 showed the highest level, 85.3%/gm, in the lung. 2. The colloid for liver scanning made with NaCl-NaOH system showed the highest liver uptake at pH 7.2, and at either higher or lower pH than 7.2 showed decrease of liver uptake more or less. 3. The activity of $^{113m}In$ eluted through $^{113}Sn-^{113m}In$ generator indicated over 90% in the initial 4 ml, and particularly 88.1%-86.0% in the initial 2 ml. 4. The incubation time, tempertaure and mechanical irritation related to colloid formation and coating of colloid were not the definite condition of influence.

증례 : 갑상선종 생검으로 검색된 전이성 악성 흑색종 1 예

고창순 ( Chang Soon Koh ),이홍규 ( Hong Kyu Lee ),노흥규 ( Heung Kyu Ro ),함의근 ( Eui Keun Ham ),고행일 ( Haeng Ill Ko ),이현순 ( Hyun Soon Lee ) 대한내과학회 1976 대한내과학회지 Vol.19 No.6

113mIn 교질에 의한 간주사에 관한 연구

홍창기 ( Chang Ki Hong ),고창순 ( Chang Soon Koh ),이종헌 ( Chong Heon Rhee ),장고창 ( Ko Chang Chang ) 대한핵의학회 1969 핵의학 분자영상 Vol.3 No.1

간 scan용 113mIn 교질을 만들어 이의 간섭취율 및 혈중 clearance을 측정하고 간 scan을 시행하여 198Au 교질의 성적과 비교관찰하여 다음과 같은 결론을 얻었다. 1) 정상인에 있어서 113mIn 교질의 간섭취율 반감시간은 1.40±0.24분 및 혈중 clearance의 반감시간은 1.47±0.27분으로 198Au 교질의 경우보다는 유의한 단축을 보였다. 그러나 이로서 간기능을 추정하는 것은 반드시 정확한 index가 되는 것 같지 않았다. 2) Space occupying lesion의 해상능은 113mIn 교질을 사용하면 198Au 교질보다 우수함을 알 수 있었다. 3) 113mIn 교질의 간섭취율은 간기능의 변화에 따르는 198Au 교질의 간섭취율의 변동과 반드시 일치하는 것은 아님을 알 수 있었으며 따라서 이는 RES의 탐식능의 다양성에 기인한 듯 하였다. 4) 113mIn 교질에 의한 간 scan에 있어서는 정상인에 있어서도 비장의 출현이 있었다. 5) 간기능장애가 있을때는 간의 교질 섭취성이 113mIn 교질에 있어서 198Au 교질보다 더욱 장애를 받으며 비장의 교질 섭취능은 상반되는 관계에 있음을 알 수 있었다. 6) 113mIn 교질을 만든 다음 인체에 사용하기 전에 coarse filter에 걸려서 사용하는 경우 더욱 좋은 간 scan상을 볼 수 있었다. 7) 간 scan에 113mIn 교질을 사용함으로서 scan시간을 더욱 단축시킬 수 있었다. There have been reported numberous cases of liver scanning in use of 198Au colloid by many investigators, however, one in use of 113mIn colloid has not been reported as yet in this country. The dose of 113mIn for high diagnostic value in examination of each organ was determined and the diagnostic interpretability of liver scanning with the use of 113mIn was carefully evaluated in comparison with the results of the liver scanning by the conventionally applied radioisotope. The comparative study of both figures of liver scanning with the use of 113mIn colloid and 198Au colloid delivered following results:1) The liver uptake rate and clearance into peripheral blood were accentuated more in case of 113mIn colloid than in case of 198Au colloid. 2) The interpretability of space occupying lesion in liver scanning with 113mIn was also superior to one with 198Au. 3) The figure of liver scanning with 113mIn colloid corresponds not always to the figure with 198Au. This difference can be explained by difference of phagocytic ability of reticuloendotherial system within liver. 4) In the liver scanning with 113mIn colloid, the spleen is also visualized even in normal examine. 5) In the cases of disturbed liver function, uptake is more decreased in use of 113mIn colloid than in 198Au, in the spleen, however, the way is contrary. 6) With use of 113mIn colloid, the time required for scanning could be shortened in comparison with 198Au. 7) The filtration of 113mIn colloid for scanning prior to human administration gives an expectation for better scanning figure.

한국인 갑상선암의 임상적 관찰

고창순 ( Chang Soon Koh ),이문호 ( Mun Ho Lee ),김용일,최태규 ( Tae Kyu Choi ),고행일 ( Haeng Ill Ko ) 대한내과학회 1975 대한내과학회지 Vol.18 No.1

循環濾過式과 流水式 飼育시스템에 의한 海産魚類 種苗生産 比較

장영진(Young Jin Chang),고창순(Chang Soon Ko),양한섭(Han Soeb Yang) 한국수산과학회 양식분과 1995 韓國養殖學會誌 Vol.8 No.2

부신피질자극호르몬 단독결핍 1 예

조보연(Bo Youn Cho),이홍규(Hong Kyu Lee),고창순(Chang Soon Koh),민헌기(Hun Ki Min),이석인(Seok In Lee),고경수(Kyung Soo Ko),홍성관(Sung Kwan Hong),김성연(Seong Yeon Kim) 대한내과학회 1992 대한내과학회지 Vol.43 No.1

Isolated ACTH deficiency is a rare disorder, only 50 cases of which have been reported worldwide till now, and characterized by hypoglycemia, hypotension and hyponatremia. In 1989, we reported the first case of Isolated ACTH deficiency combined with autoimmune thyroiditis. Recently we experienced another case of Isolated ACTH deficiency without other combined autoimmune disease or other endocrine abnormalities. The patient, 31 year-old man, was admitted because of dyspnea, nausea and vomiting. On admission, serum sodium was 121mmol/L, pottasium 2.6mmol/L, serum osmolality 239mOsm/kg and urine osmolality 737mOsm/kg. Basal serum cortisol was 1.0 ↓ ug/ml, ACTH 13 pg/ml and glucose was 43 mg/dl. Although cortisol did not respond to rapid ACTH stimulation, it responded to standard ACTH stimulation test, On combined pituitary stimulation test, the cortisol did not respond and all anterior pituitary hormones showed normal responses except ACTH. There were no abnormalities in Sellar CT, and hyponatremia was corrected dramatically with prednisolone replacement.

복합갑상선홀몬지수의 진단적 가치

김광회 ( Kwang Hoi Kim ),한승수 ( Seung Soo Han ),이홍규 ( Hong Kyu Lee ),서재현 ( Jae Hyun Seo ),고창순 ( Chang Soon Ko ),홍기석 ( Ki Suck Hong ),임정순 ( Jung Soon Lim ) 대한내과학회 1979 대한내과학회지 Vol.22 No.8

당뇨유발 백서에서 △6 탈포화효소의 활성도 및 지방산 구성

신찬수(Chan Soo Shin),이문규(Moon Kyu Lee),한은경(Eun Kyung Han),고경수(Kyung Soo Ko),김철희(Chul Hee Kim),김성연(Seong Yeon Kim),조보연(Bo Youn Cho),이홍규(Hong Kyu Lee),고창순(Chang Soon Koh),민헌기(Hun Ki Min) 대한내과학회 1992 대한내과학회지 Vol.43 No.2

Microsomal △6 desaturase is a key enzyme in the metabolism of polyunsaturated fatty acids, the activity of which is regulated by insulin. To determine if changes in d △6 desaturase activity in experimental diabetes correspond to changes in fatty acid compoistion of microsome and RBC membrane, 18 Sprague-Dawley rats were studied for 6 weeks. The animals were divided into 3 groups with 6 rats in each group. The first group was the contrals, the 2nd 3rd groups were streptozotocin diabetic rats but insulin was injected to the 3rd group. The △ 6 desaturase activities of diabetic rats were 68pg of those of controls (p<0.05) and increased to 119% of controls (p<0.05) with insulin treatment. Linolic (LA), palmitic (PA) and docosahexaenoic acids (DHA) were increased and arachidonic (AA) & oleic acids (OA) decreased in liver microsome of diabetic rats, which were reversed with insulin treatment, respectively. In erythrocyte membrane LA & stearic acids (SA) were increased, and PA, AA, and palmitoleic acids were decreased which were not reversed with insulin treatment, however. To summarize, insulin controls the △ 6 desaturase activities, which in turn affect fatty acid compositions of microsome and RBC membranes. Theee findings suggest that adequate insulin therapy is required for the correction of the polyunsaturated fatty acid metabolism as well as blood glucose control in the insulin dependent diabetics.

99m - Tc DISIDA Scan 을 이용한 정상인과 담석증 환자에서의 담낭역학에 관한 연구

윤용범(Yong Bum Yoon),박용현(Yong Hyun Park),이영철(Young Cheol Lee),정준기(Joon Ki Jung),고창순(Chang Soon Ko) 대한소화기학회 1987 대한소화기학회지 Vol.19 No.1

N/A Gallbladder stasis has been implicated as one of factors in gallstone formation. To evaluate the gallbladder dynamics, gallbladder filling and emptying were quantitated by the computer assisted 99m-Tc DISIDA scan in 8 normal subjects and 23 patients with gallstones. Gallstone patients exhibited normal gallbladder filling rate, and there seemed to be three periods (Lag period, Rapid contraction period, Slow contraction period) in gallbladder contraction induced by a fatty meal. In patients with gallstones, ejection fraction in whole contraction period, ejection fraction and ejection rate in rapid contraction period of gallbladder were reduced (p<0.05) when compared with controls. On closer inspection, the gallstone patients fell into two subgroups, separated by ejection fraction 72%(x-2S.D. of control). There were no significant differences in opacity, number, size of gallstones and biliary colic episodes between these two subgroups. When we divided 3 contraction parameter which were significant differences between control and gallstone group into two subgroups by E.F. 72%, E.F.a 38.9%, E.R.a 2.9%/min (x-2S.D. of control) and compared them with gallbladder wall thickness by ultrasonography, the thicker gallbladder wall brought about the decreased ejection fraction and ejection rate in rapid contraction period, but the ejection fraction in whole emtying period was not influenced by gallbladder wall thickness. To elucidate the relationship between gallbladder inflammation and contraction, Spearmans rank correlation analysis between inflammatory scorings of chronic cholecystitis and several parameters of gallbladder contraction was used; the linear relationships between two groups were not found (p> 0,05), but owing to small number of cases the result was hard to be accepted. Thus defective gallbladder emptying is evident in a subgroup of gallstone patients and is indepen- dent of clinical features, stone size, number and opacity. The gallbladder wall thickness influenced to ejection fraction and ejection rate in rapid contraction period, but not to whole ejection fraction. Relationship between gallbladder inflammation and gallbladder contractions was not elucidated.