악성 임파종에서 혈청 β2-microglobulin 에 관한 연구

고은희,김현숙,한지숙,김주항,이삼열,김병수,고윤웅,윤진우,전상일 대한내과학회 1986 대한내과학회지 Vol.30 No.4

Rata 2 microglobulin, a low molecular weight polypeptide, exists in the plasma membrane of all nucleated cells and its normal serum concentration, in free form, is in the range of 1, 500 to 2,000 μg/L, Serum β2-microglobulin is increased in malignant tumors especially in B-cell lymphopro-liferative disorders, and thus the serum concentration is of help for the diagnosis, therapeutic response and prognosis of these disorders. The author attempted to correlate the serum β2-microglobulin levels and the clinical courses of malignant Iymphomas, in 35 patients with malignant lymphoma showing normal BUN and serum creatinine levels. These patients were collected from the in-patients of Severance Hospital, Yonsei Medical Center during September 1984 to July 1985. As a control group, the serum β2-microglobulin levels of 38 healthy subjects were also measured. The following results were obtained: 1) The serum β2-microglobulin in 38 healthy subjects was 1,827±546 μg/L. The serum β2-mic-roglobulin of 35 patients with malignant lymphoma revealed 2.298±1.117μg/L. The serum p, microgiobulin in 35 patients with malignant lymphoma was higher than that of control group significantly(p$lt;0.05). 2) The serum β2-microglobulin level according to clinical stage showed increasing tendency with higher with higher stages. Fourteen patients of stage I or II showed the mean serum β2-microglobulin level of 1,660±688 μg/L and 19 patients of stage g or p showed 2,754±1,196 μg/L. The statistical difference he tween those 2 groups was significant(p$lt;0.005), The serum β2-microglobulin was increased($gt;2,000ug/L) in 35.7% of stage I and g, and 77.8% of stage g and IV untreated active malignant lymphoma. Among the non-Hod-gkin's lymphoma group(n=29), serum β2-microg-lobulin was increased in 35.7% of patients of stage I and in 78.5% of those of stage III and IV. The same increased level was observed in 75.0Fo of Hodgkin's disease(n=4) of stage III and IV. 3) The serum β2-microglobulin level of 16 patients with the systemic symptoms(B symptom) was 2,870±1,203ug/L and 17 patients without B symptom was 1,744±765ug/L(pg0,005). 4) In 13 Patients. serum β2-microglobulin levels were monitored prior to and after the treatment. Six of these patients were responsive to the treatment and their serum β2-microglobulin levels prior to treatment were rather lower than normal. Serum β2-microglobulin level decreased as their clinical courses improves. On the other hand, the other 7 patients who had considerably high serum β2-microglobulin levels showed the increasing tendency of its levels inspite of the treatment. Based on these studies, it can be suggested that the serum β2-microglobulin determination is a valuable laboratory study in following up malignant lymphoma.

베시클 교란 화합물들의 포스포리파제 D 활성에 미치는 영향

고은희 덕성여자대학교 자연과학연구소 2004 자연과학 논문집 Vol.10 No.-

본 실험에서는 베시클 교란 화합물들의 존재 하에서 양배추 phospholipase D (PLD)의 활성을 phosphatidyl choline (PC) small unilamellar vesicle (SUV)을 기질로 하여 측정하였다. 방법으로는 PLD 가수분해 생성물인 phosphatidic acid를 pH-stat titration 방법으로 결정하였다. 검토한 베시클 교란물질 중 phosphatidic acid와 oleate는 양배추 PLD를 활성화시켰으며, Iysophosphatidylcholine는 PLD활성을 저해시켰다. 이들 결과는 인지질 베시클의 물리적 변화가 양배추 PLD 활성 측정에 큰 영향을 미치고 있음을 보여주고 있다. Phospholipase D (PLD) activity, using phosphatidylcholine vesicles as substrate, was determined in the presence of vesicle-perturbing chemicals by measuring the hydrolysis product, phosphatidic acid, by pH-stat titration. Among the vesicle-perturbing chemicals examined, phosphatidic acid and oleate showed stimulating effects on cabbage PLD activity whereas lysophosphatidyl choline inhibited the PLD activity. These results suggest that PLD activities are affected profoundly by altering the physical property of the phospholipid vesicles.

호텔 종사자가 지각하는 CSR 동기와 진정성이 조직시민행동에 미치는 영향 - CSR 진정성의 매개 역할과 세대의 조절 역할 -

고은희,조민호 한양대학교 관광연구소 2019 觀光硏究論叢 Vol.31 No.1

본 연구는 호텔 종사자가 지각하는 CSR 동기와 진정성이 조직시민행동에 미치는 영향을 통합적으로 파악하고자 하였다. 이를 위해 첫째, 선행연구와 이론적 고찰을 통해 연구모형을 구축하였다. 둘째, 국내8개 특급호텔종사자에게 실증조사를 실시한결과 최종적으로 유효한 253부의 자료를 확보하고 구조방정식 모델을 통해 이를 분석하였다. 셋째, 분석의결과는 다음과 같다. 호텔종사자가지각하는CSR 동기는CSR 진정성에 부분적으로 유의한영향을 미치는것으로 나타났다. 호텔 종사자가 지각하는 CSR 진정성은 조직시민행동에 유의한 정(+)의 영향을 미치는 것으로 나타났다. 호텔 종사자가 지각하는 CSR 동기와 조직시민행동 간의 관계에서 CSR 진정성의 매개 효과는 부분적으로 존재하는 것으로 나타났다. 세대는 CSR 진정성과 조직시민행동 간의 관계에서 조절역할을 하는 것으로 나타났다. 이러한 결과를 통해 변수 간의 유의미한 상호 관련성과 특히 CSR 동기와 진정성이 종사자의 조직시민행동을 향상시키는데 기여한다는 점을 밝힘으로써 호텔의 지속가능경영과효과적인 조직관리 측면에서 이들의 중요성을 확인할 수 있었다. 끝으로 연구의 결과를 바탕으로 학문적 및 실무적으로 유용한 시사점을 제시하였다. This study aims to examine the impact of hotel employees’ perceived CSR motives and CSR authenticity on organizational citizenship behavior. To achieve the research objective, first, a conceptual model was proposed based on a literature review. Second, the model was empirically analyzed via the structural equation modeling by AMOS using valid data, collected from current 253 employees of 8 deluxe hotels in South Korea. Third, analysis results showed that perceived CSR motives had partial influences on perceived CSR authenticity. CSR authenticity had a positive influence on organizational citizenship behavior. CSR authenticity partially had a mediation effect in the relationship between CSR motives and organizational citizenship behavior. A moderating effect of generational differences was revealed in the relationship between CSR authenticity and organizational citizenship behavior. These findings identified certain interrelationships among variables, and in particular, the importance of both CSR motives and authenticity in terms of hotels’ sustainable management and effective organizational management by confirming their impact to employees’ organizational citizenship behavior. Finally, theoretical contributions and practical implications were discussed.

양배추 포르포리파제 D 활성부위의 히스티딘 잔기 특성연구

고은희 덕성여자대학교 자연과학연구소 1997 자연과학 논문집 Vol.3 No.-

포스포리파제 D의 활성 부위에 존재하는 히스티딘을 확인하기 위해 히시티딘을 선택적으로 변형시키는 디에틸피로카르본산(DEP)을 사용하였다. 변형된 효소의 활성도는 0.5 mM DEP에서 90% 감소하였다. 240 ㎚ 에서의 흡광도 차이로부터 이 농도에서 효소 1몰당 1.5 개의 히스티딘 잔기가 변형된다고 제안할 수 있다. 기질중의 하나인 포스파티딜콜린은 효소를 DEP에 의한 비활성화로부터 보호하며, 이는 DEP가 활성 부위를 변형한다는 것을 가리킨다. [^14C] DEP에 의해 변형된 효소를 Endoproteinase Arg-C로 처리하여 한개의 방사능을 가진 펩티드를 얻었다. 이 방사능을 가진 펩티드의 서열은 Met-Ile-Tyr-Phe-Gly-Lys-His이었다. 이 결과는 포스포리파제 D활성 부위에 히스티딘 잔기가 있다는 직접적인 증거가 된다. As part of a characterization of the active site of phospholipase D (PLD), the histidine residue in cabbage PLD was modified with diethyl pyrocarbonate (DEP). The activity of the modified PLD was inhibited by 90 % at 0.5 mM DEP. The differential absorption at 240 ㎚ could suggest that at this concentration the modified histidine residues were 1.5 ㏖ per PLD. One of substrates, phosphatidylcholine protected the enzyme from inactivation by DEP, thereby indicating that the DEP modified the active site histidine. Endoproteinase Arg-C digestion of [^14C] DEP-modified enzyme yielded a single radioactive peptide. The sequence of this radioactive peptide was Met-Ile-Tyr-Phe-Gly-Lys-His. This data provides a direct evidence for the presence of histidine residue in the active site of PLD.

고등학교에서의 극한의 개념 지도에 관한 연구

고은희,송석준 濟州大學校 師範大學 科學敎育硏究所 1998 科學敎育 Vol.15 No.-

In this thesis, we research the definitions and some examples on the limits of both sequences and functions from mathematics textbooks that used in high schools and in universities, respectively. And then we compared them and found some difference between them. At last, we tried to correct the defects of definitions in the high school textbooks and to introduce the strict definitions of limits of both sequences and functions into high school textbooks.

정모두부방사선사진 촬영시 두부의 수직회전에 따른 투사오차

고은희,이기헌,황현식,성재현 전남대학교 치의학연구소 2002 구강과학 Vol.14 No.4

This study was performed to find out how much projection errors in the cephalometric measurements were made by vertical head rotation in taking posteroanterior cephalograms. 25 adults without any apparent facial asymmetry or severe sagittal skeletal discrepancy were selected and the posteroanterior cephalograms were taken with the head rotated 5˚, 10˚ superior and inferior each to the reference position(0˚), The 7 height. 5 width and 6 angular measurements were taken at each 5 positions. Through the statistical analysis of all measurements taken at each rotated position, following results were obtained. 1. The projection errors of height measurements were remarkably larger than those of width or angular measurements. 2. Among the height measurements, the farther to the rotation axis the measurements were. the larger the projection errors were. 3. Among the width measurements. mandibular width and intermolar width of mandibular first molars showed significant differences between the values taken at each rotated position. while nasal width, maxillary width and intermolar width of maxillary first molars did not. 4. Among the angular measurements. the angle between horizontal reference line and the line that is connected to crista galli and antegonion or maxillare showed significant differences between the values taken at each rotated position. The above results suggest that it is needed to the effort to keep constant head rotation for taking the useful posteroanterior cephalogram, because projection errors are caused by vertical head rotation.

pH-stat 적정법을 이용한 포스포리파제 D 활성도 측정

고은희,손영신 덕성여자대학교 자연과학연구소 2000 자연과학 논문집 Vol.6 No.-

Cabbage phospholipase D(PLD)의 활성도를 pH-stat titration 방법과 분광학적 방법으로 비교하여 검토하였다. 이를 바탕으로 PLD 효소 활성화에 기능성 식품이 미치는 영향의 검토에 pH-stat titration 방법의 적용가능성을 제시하고자 한다. PLD 효소의 기질은 phosphatidylcholine small unilamellar vesicle (PC SUV)를 사용하였다. PLD 효소 반응 생성물중에 하나인 phosphatidic acid(PA)를 측정하는 pH 적정법과 나머지 생성물인 choline을 측정하는 분광학적방법은 서로 일치하는 결과를 보여주었다. 따라서 기능성 식물 추출물의 색소 존재 유무에 방해를 받지 않는 pH-stat 적정법은 포스포리파제 D 활성도 측정에 훨씬 실용적인 측면이 있는 실험법이 될 것이다. The activity of cabbage phospholipase D (PLD) has been measured by pH-stat titratㅑon of the PA, one of the enzymatic reaction product, and the results were compared with those from the spectroscopic method. When phosphatidylcholine small unilamellar vesicle (PC SUV) was used as a substrate for phospholipase D, the results from the pH-stat titraton and spectroscopic method agree well within experimental error. Since the pH-stat titration is free from the interference of colored plant pigments, we suggest that the pH-stat titration is a better altenative method in examining the effect of plant extract on the PLD activity.

성장과 노화에 따른 식물의 PLD의 Immunoblotting 분석

고은희 덕성여자대학교 자연과학연구소 1997 자연과학 논문집 Vol.3 No.-

양배추 PLD 항체를 이용한 immunoblotting 방법에 의해 여러 식물조직에서 PLD의 존재와 변화를 관찰하였다. 검토한 식물중 메주콩, 당근, 완두콩에서 쉽게 PLD를 확인할 수 있었다. 식물성장과 PLD양의 변화를 관찰하기 위하여 완두콩을 27℃에서 발아시키면서 PLD양의 변화를 관찰하였다. 발아시작 1일후 최고치를 나타내고 2일부터는 감소하여 3일후에는 최대치의 50%값을 보였다. 식물의 노화 현상을 보기 위해 양배추 잎을 24℃ 물에 저장하였을때 PLD양이 점점 증가하여 6일후에는 초기보다 30%이상 증가된 값을 가졌다. 한편 0.05 M의 Ca^2+는 PLD양의 증가를 둔화시켰으며 6일후에는 Ca^2+가 없을때보다 PLD 증가를 50%이상 둔화시켰다. 이들 결과로부터 식물의 발아와 노화에 모두 PLD 활성이 관여함을 알 수 있다. Phospholipase D from several plant tissues were identified and analyzed by immunoblotting method using cabbage PLD antibody. Among those tested, PLD from carrot, green pea, and soybean showed specific binding with cabbage PLD antibody. PLD in green pea was analyzed during the germination at 27℃. The level of PLD reached the highest level on day 1, and decreased gradually to the 50% of the maximum value on the third day of germination. PLD on senescence was studied using cabbage leaf stored in 24℃ H_2O. The PLD level increased gradually up to 30% at the 6th day on storing. The presence of Ca^2+ retarded the PLD increase up to 50% on the 6th day. The present results suggest that PLD activity is involved in both seed germination and plant leaf senescence.

교정용 와이어 및 브라켓에 이산화티탄 광촉매 코팅 시 코팅방법에 따른 비교연구

고은희,조진형 대한치과교정학회 2006 대한치과교정학회지 Vol.36 No.6

본 연구는 항균 교정장치의 개발에 도움이 되고자 이산화티탄 광촉매의 코팅 시 안정적이고 효과적인 방법을 찾기 위하여 시행되었다. 시판되고 있는 교정용 와이어와 브라켓에 sol-gel법, CVD (Chemical Vapor Deposition)법 및 PE-CVD (Plasma Enhanced-CVD)법으로 이산화티탄을 각각 코팅한 다음 각 방법으로 코팅된 이산화티탄 박막의 특성을 알아보고자 주사전자현미경을 이용하여 각 시편의 코팅박막 표면의 거칠기를 관찰하였고 adhesive tape pull test를 이용하여 코팅박막의 접착강도를 측정하였다. 메틸렌블루용액에 각 시편을 침지시킨 후 시간경과에 따른 메틸렌블루용액의 농도변화 측정을 통해 코팅박막의 분해능을 평가하였으며 불화나트륨 용액에 각 시편을 침지시킨 후 주사전자현미경을 이용하여 표면부식 정도를 관찰함으로써 불소화합물에 대한 내부식성을 평가하여 다음과 같은 결과를 얻었다. 코팅박막의 표면은 CVD법 및 PE-CVD법이 sol-gel법이나 코팅되지 않은 시편에 비해 더 매끄러웠다. 코팅박막의 접착강도는 PE-CVD법이 가장 높았고, CVD법, sol-gel법의 순으로 낮게 나타났다. 코팅박막의 메틸렌블루 분해능은 PE-CVD법이 가장 높았고, CVD법, sol-gel법의 순으로 낮게 나타났다. 코팅박막의 불소화합물에 대한 내부식성은 CVD법 및 PE-CVD법이 sol-gel법에 비해 높게 나타났다. 이상의 결과는 교정용 와이어 및 브라켓의 이산화티탄 광촉매 코팅 시 CVD법 및 PE-CVD법이 sol-gel법보다 적절한 방법임을 시사하였다. The purpose of this study was to search for an appropriate method of coating TiO_(2) on orthodontic appliances. TiO_(2) thin films were deposited on orthodontic wires and brackets using sol-gel, CVD(Chemical Vapor Deposition) and PE-CVD (Plasma Enhanced-CVD) methods. The roughness of TiO_(2)-coated surfaces was investigated via scanning electron microscope (SEM) and adhesive strength of TiO_(2) thin films was measured by adhesive tape pull test. Methylene blue degradation test was carried out to evaluate the photocatalytic activity of TiO_(2) and the corrosion resistance of TiO_(2) thin films against fluoride solution was also analyzed by observing the surfaces of TiO_(2)-coated wires and brackets via SEM after immersion in sodium fluoride solution. Through the comparison of properties and photocatalytic activity of TiO_(2) thin films according to the coating methods, the following results were obtained. Smoother surfaces of TiO_(2) thin films were generated by CVD or PE-CVD methods than through the sol-gel method or the control. Adhesive strength of the TiO_(2) thin films was highest in PE-CVD and gradually became lower in the order of CVD, then the sol-gel method. Photocatalytic activity of TiO_(2) thin films on methylene blue was the highest in PE-CVD and gradually became lower in the order of CVD, then the sol-gel method. Corrosion resistance of TiO_(2) thin films against fluoride solution was stronger in CVD and PE-CVD methods than in the sol-gel method. The results of this study suggest that the CVD or PE-CVD methods is more appropriate than the sol-gel method for TiO_(2)coating on orthodontic wires and brackets.

쥐 뇌 Arylsulfatase Bm의 분리 및 특성연구

고은희 德成女子大學校 1985 德成女大論文集 Vol.14 No.-

쥐 뇌로부터 Arysulfatase Bm을 부분정제하여 그 특성을 연구하였다. 정제는 황산암모늄 분별침전, DEAE-Cellulose 크로마토그라피, Sephadex G-200겔 여과법, ConA-Sepharose 4B 친화크로마토그라피 단계를 거쳐 이루어졌으며 약 100배의 정제도와 17%의 회수율을 나타냈다. Arylsulfatase Bm의 일반적 특성을 p-nitrophenyl sulfate를 기질로 사용하여 연구되었다. 기질의 농도가 각각 2.5mM, 5mM, 25mM일때 효소활동도와 반응시간의 관계는 75분까지는 직선비례로 나타났다. 반응속도와 기질농도의 관계는 비정상적인 두개의 상을 갖는 곡선으로 나타났으며 기질의 농도가 대략 40mMdlfEo 반응속도는 감소를 보였다가 다시 증가하였다. 여기에서 나타나는 비정상적인 속도대 농도곡선은 현 시점에서는 설명할 수가 없으며 40mM 농도까지인 첫번째 곡선 부분에서 얻어진 속도 매개 변수 V??과 K??은 각각 22.73μ/min/mg 단백질, 83.5mM이었다. Arylsulfatase Bm은 pH 5.6과 6.4 두개의 최적 pH를 갖는 것으로 나타났다. Ca², Mn², Mg²양이온은 효소작용을 억제했으며 생성물중의 하나인 황산음이온도 효소작용을 저해 하는 것으로 나타났다. 황산이온의 영향은 일반적인 생성물에 의한 효소활동도 저해라고 생각할 수 있겠으나 또 하나의 생성물이라고 할 수 있는 페놀은 아무런 영향을 나타내지 않았다. 계면활성제 SDS와 Triton X-100는 효소작용을 활성화 시켰으며 특히 Triton X-100는 그 농고가 0.5%일 때 10배 이상의 활동도 증가를 보여주어 특기할만하다. 이들 실험결과들은 앞으로의 Arylsulfatase Bm의 생화학적 성질을 자세히 규명하기 위한 연구의 기초자료로 사용될 수 있을 것으로 사려된다. Arylsulfatase(Arylsulfate sulfohydrolase, EC. 3.1.6.1.) Bm from rat brain was partially purified and characterized. The purification steps involved ammonium sulfate fractionation, DEAE-cellulose ion exchange chromatography, gelfiltration on Sephades G-200, and concanavalin A-Sepharose 4B affinity chromatography. Arylsulfatase Bm was purified 100-fold with 17% recovery. The general properties of Arylsulfatase Bm was investigated with p-nitrophenyl sulfate as substrate. When the concentration of substrate was either 2.5mM, 5mM, or 25mM, the activity of the enzyme vs. the incubation time showed a linearity up to the 75min. The reaction rate vs. the suvstrate concentration exhibits an unusual shaped curve with two distinctive phases. Invariably the reaction rate of enzyme leveled off at about 40mM substrate and again increased steadily up to 100mM. The reason for this unusual rate vs. concentration relationship cannot be explained satisfactorily at this point. The kinetic parameters M?? and K?? were found to be 22.73μmole/min/mg protein and 83.5mM respectively for the first phase of the rate vs. concentration curve. Arylsulfatase Bm has two optimum pH, 5.6 and 6.4 under the experimental condition. Cations such as Ca²??, Mn²??, and Mg²?? inhibit the enzyme activity. Sulfate anion, which is one of the products, inhibits the enzyme activity probably due to the product inhibition. But phenol has no effect on the enzyme activity under the experimental conditions employed. Detergents, SDS and Triton X-100, exhibit a remarkable activation of the enzyme. A cioser investigation of the effect of Triton X-100 on the enzyme activity showed that the enzyme was activated approximately 10 fold when the incubation mixture contained 0.5% Triton X-100. The data presented here can be utilized as basic materials for the biochemical studies of Arylsulfatase Bm in order to elucidate the biochemical characteristics of this enzyme.