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Native 및 Denatured Calf Thymus DNA의 DNase 1 에 대한 Susceptibility에 미치는 Spermine의 영향
고동성,허준,이천배,박문규,Thong-Sung Ko,Joon Huh,Chun-Bae Lee,Moo- Kyeu Park Korean Chemical Society 1983 대한화학회지 Vol.27 No.6
Spermine은 DNase 1에 대한 native 및 denatured calf thymus DNA의 susceptibility에 상반된 효과를 갖는다. spermine은 그 native DNA의 susceptibility를 증가시키지만 그 denatured DNA의 것은 감소 시킨다. spermine이 존재하지 않을 경우에는 그 deoxyribonuclease의 반응성은 native 및 denatured DNA의 conformation차이에 외견상 무감각한 것으로 나타난다. Spermine has opposite effect on the susceptibility of native and denatured calf thymus DNA toward deoxyribonuclease Ⅰ (DNase I; deoxyribonucleate 5'-oligonucleotidohydrolase; EC 3.1.4.5; from bovine pancreas). It enhances the susceptibility of the native DNA, whereas depresses that of the denatured DNA. In the absence of spermine, the reactivity of the deoxyribonuclease is apparently insensitive to the conformational difference of the native and denatured DNA.
고동성,서일환,이천배,서정인,Ko, Thong-Sung,Suh, Il-Hwan,Lee, Chun-Bae,Suh, Jeong-Ihn 생화학분자생물학회 1987 한국생화학회지 Vol.20 No.1
스페르민 존재하에서 nalidixic acid 및 oxolinic acid 등의 음이 온성 quinolone 항생제는 ethidium bromide, proflavine 및 acridine orange 등의 양이온성 intercalative 리간드들과 마찬가지로 calf thymus DNA의 DNase 1에 대한 가수분해 반응성을 증가 시켰다. 그러나 본성 DNA와는 대조적으로 변성 DNA의 가수분해 반응성은 이들 양이온성 물감분자들에 의해서도 그리고 음이온성 quinolone 항생제들에 의해서도 영향 받지 않았다. 또한 스페르만 부재시에는 본성 DNA의 가분해 반응성도 이들 음이온성 drug에 의하여 영향 받지 않았다. 이들 데이터는 음이온성 quinolone 항생제가 스페르민과 intercalative 복합체를 형성하며 따라서 quinolone 항생제가 DNA conformation probe로서 사용될 수 있음을 시사하여 준다. 그러나 스페르만과 같은 polycation 없이 음이 온성 quinolone 항생제 자체만으로는 intercalation이 불가능함을 알 수 있다. 이와같은 이해는 분자궤도함수론적 고찰에 의하여 확인된다. The hydrolytic susceptibility of calf thymus DNA to DNase 1 was enhanced by such anionic Quinolone antibiotics as nalidixic acid and oxolinic acid in the presence of spermine, as well as by such cationic intercalative agents as ethidium bromide, profavine, and acridine orange. In contrast with the susceptibility of the native DNA, the susceptibility of the denatured DNA was not affected by the quinolone antibiotics, not either by the cationic dyes. Even the native DNA, however, was not influenced by the anionic antibiotics in its susceptibility to DNase 1 if spermine was omitted. The data here may lead to a tentative concusion that the quinolone antibiotics may form intercalative complexes with spermine, and that thereby the quinolone antibiotics can be used as probes of DNA conformation. whereas the anionic antibiotics alone without spermine may not have the intercalation activity. as supported by the MO data.
스페르민 - DNA 복합체의 이례적 흡광도 - 온도 양상에 미치는 DMSO 의 영향
고동성,이천배,최우영,이찬용 ( Thong Sung Ko,Chun Bae Lee,Woo Young Choi,Chan Young Lee ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.1
In an effort to improve our knowledge on the mechanism of the collapse of DNA into a compact structure by polyamines, we investigated the effect of dimethyl sulfoxide (DMSO), a hydrophobic environmental reagent on the anomalous absorbance-temperature profile of condensed DNA, induced by spermine and having a trough preceeded by the initiation of the melting phase. The trough peak size can be decreased by increased concentration of DMSO, and the cooperativity of the phase transition to the region in the anomalous absorbance-temperature profile is more sensitive to DMSO than that of the melting region of the profile. The present data indicate that the anomalous absorbance-temperature profile is due to the thermal stabilization of the tertiary structure of the spermine-induced collapsed state of DNA and the importance of hydrophobic interaction in the stabilization of the condensed state of DNA induced by spermine.
고동성,서일환,이천배,서정인 ( Thong Sung Ko,Il Hwan Suh,Chun Bae Lee,Jeong Ihn Suh ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.1
The hydrolytic susceptibility of calf thymus DNA to DNase 1 was enhanced by such anionic Quinolone antibiotics as nalidixic acid and oxolinic acid in the presence of spermine, as well as by such cat-ionic intercalative agents as ethidium bromide, profavine, and actidine orange. In contrast with the susceptibility of the native DNA, the susceptibility of the denatured DNA was not affected by the quinolone antibiotics, not either by the cationic dyes. Even the native DNA, however, was not influenced by the anionic antibiotics in its susceptibility to DNase 1 if spermine was omitted. The data here may lead to a tentative contusion that the quinolone antibiotics may form intercalative complexes with spermine, and that thereby the quinolone antibiotics can be used as probes of DNA conformation, whereas the anionic antibiotics alone without spermine may not have the intercalation activity, as supported by the MO data.
스페르민-DNA 복합체의 이례적 흡광도-온도 양상에 미치는 DMSO의 영향
고동성,이천배,최우영,이찬용,Ko, Thong-Sung,Lee, Chun-Bae,Choi, Woo-Young,Lee, Chan-Young 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.1
DNA의 응축을 유발시키는 spermine 농도 존재시 downward peak(trough)를 갖는 이례적 흡광도-온도 양상에 hydrophobic environment를 조성하는 DMSO의 영향을 조사하였다. DMSO 농도증가에 따라 downward peak의 깊이가 점차 얕아졌으며 그 phase transition enthalpy와 relative cooperative length(n)가 melting region보다는 downward peak(trough) phase transition에 더 예민하게 영향을 받았다. 따라서 본 data는 spermine에 의하여 유발되는 응축된 DNA 구조에 이르는 hydrophobic interaction에 의한 tertiary structure 형성이 온도상승에 따라 유리해짐으로써 downward peak가 생성된다는 추리를 확인하는 실험결과가 될 것이다. In an effort to improve our knowledge on the mechanism of the collapse of DNA into a compact structure by polyamines, we investigated the effect of dimethyl sulfoxide (DMSO), a hydrophobic environmental reagent. on the anomalous absorbance-temperature profile of condensed DNA, induced by spermine and having a trough preceeded by the initiation of the melting phase. The trough peak size can be decreased by increased concentration of DMSO, and the cooperativity of the phase transition to the region in the anomalous absorbance-temperature profile is more sensitive to DMSO than that of the melting region of the profile. The present data indicate that the anomalous absorbance-temperature profile is due to the thermal stabilization of the tertiary structure of the spermine-induced collapsed state of DNA and the importance of hydrophobic interaction in the stabilization of the condensed state of DNA induced by spermine.
고동성,서일환,이천배,서정인 충남대학교 기초과학연구소 1987 연구논문집 Vol.7 No.-
스페르민 존재하에서 nalidixic acid 및 oxolinic acid 등의 음이온성 quinolone 항생제는 ethidium bromide, proflavine 및 acridine orange 등의 양이온성 intercalative 리간드들과 마찬가지로 calf thymus DNA의 DNase 1에 대한 가수분해 반응성을 증가 시켰다. 그러나 본성 DNA와는 대조적으로 변성 DNA의 가수분해 반응성은 이들 양이온성 물감분자들에 의해서도 그리고 음이온성 quinolone 항생제들에 의해서도 영향 받지 않았다. 또한 스페르민 부재시에는 본성 DNA의 가분해 반응성도 이들 음이온성 drug에 의하여 영향 받지 않았다. 이들 데이터는 음이온성 quinolone 항생제가 스페르민과 intercalative 복합체를 형성하며 따라서 quinolone 항생제가 DNA conformation probe로서 사용될 수 있음을 시사하여 준다. 그러나 스페르민과 같은 polycation없이 음이온성 quinolone 항생제 자체만으로는 intercalation이 불가능함을 알 수 있다. 이와같은 이해는 분자궤도함수론적 고찰에 의하여 확인된다. The hydrolytic susceptibility of calf thymus DNA to DNase 1 was enhanced by such anionic Quinolone antibiotics as nalidixic acid and oxolinic acid in the presence of spermine, as well as by such cationic intercalative agents as ethidium bromide, profavine, and acridine orange. In contrast with the susceptibility of the native DNA, the susceptibility of the denatured DNA was not affected by the quinolone antibiotics, not either by the cationic dyes. Even the native DNA, however, was not influenced by the anionic antibiotics in its susceptibility to DNase 1 if spermine was omitted. The data here may lead to a tentative concusion that the quinolone antibiotics may form intercalative complexes with spermine, and that thereby the quinolone antibiotics can be used as probes of DNA conformation, whereas the anionic antibiotics alone without spermine may not have the intercalation activity, as supported by the MO data.
疎水性 環境이 Spermine에 의하여 誘發되는 凝縮된 DNA의 異例的 吸光度-溫度樣相에 미치는 影響
高東成,李天培,崔宇永,李璨容 충남대학교 1985 忠南科學硏究誌 Vol.12 No.2
We have investigated the effect of hydrophobic environmental reagents (urea and DMSO) on the anomalous absorbance-temperature profile of condensed DNA, induced by spermine. The cooperativity of the phase transition to the trough region in the anomalous absorbance-temperature profile is more sensitive to the hydrophobic environmental reagents than that of the melting region, and the trough peak size can be decreased by increased concentration of the hydrophobic reagents. The present data indicate the importance of hydrophobic interaction in the stabilization of the condensed state of DNA induced by spermine, and that the anomalous absorbance-temperature profile is due to the thermal stabilization of the tertiary structure of the spermine-induced collapsed state of the DNA.
고동성,이천배,명평근 충남대학교 기초과학연구소 1984 연구논문집 Vol.5 No.-
Spectroscopic investigations of spermine effects on the ethidium-DNA binding equilibrium, and on the absorbance-temperature profile of the DNA in the presence of ethidium bromide at various concentrations were performed, as the ethidium binding may be used as the probe of the DNA interactions with environmental ligands. The cooperativity, enthalpy, and the midpoint of the phase transition to the trough region, of the anomalous absorbance-temperature profile, which occurs at the spermine concentration where the DNA collapses into a compact structure, are more sensitive to ethidium than the Tm region phase, and the trough can be eliminated by increased concentration of ethidium.
高東成,李天培,崔宇永,李璨容 충남대학교 기초과학연구소 1986 연구논문집 Vol.6 No.-
We have investigated the effect of hydrophobic environmental reagents (urea and DMSO) on the anomalous absorbance-temperature profile of condensed DNA, induced by spermine. The cooperativity of the phase transition to the trough region in the anomalous absorbance-temperature profile is more sensitive to the hydrophobic environmental reagents than that of the melting region, and the trough peak size can be decreased by increased concentration of the hydrophobic reagents. The present data indicate the importance of hydrophobic interaction in the stabilization of the condensed state of DNA induced by spermine, and that the anomalous absorbance-temperature profile is due to the thermal stabilization of the tertiary structure of the spermine-induced collapsed state of the DNA.