Vitamine E 투여 및 식이제한과 운동이 쥐의 산화스트레스 후 대식세포능 및 혈청 MDA에 미치는 영향

강청훈,고성경 대한스포츠의학회 2001 대한스포츠의학회지 Vol.19 No.1

유산소성 운동능력이 중년 성인병 남성의 혈중 지질에 미치는 영향

강청훈,유연식 空軍士官學校 2003 論文集 Vol.51 No.-

To study effects of cardioventricular fitness and serum lipoprotein on physical activity in normal and degenerative disease groups, 36 non who aged 40-60yrs were tested by medical process and exercise stress testing. Subjects were classified into five groups that were control, activity, obesity, hypertension and diabetes group. Results of this study are following, 1. %Body fat is 19.7% in control groups, 17.4% in activity group, 30.1% in obesity group, 26.0% in hypertension group and 27.1% in diabetes group. Control and activity group are significantly leaner than obesity, hypertension and diabetes group(p<0.05). 2. In serum cholesterol, total cholesterol is 179.0㎎/㎗ in activity group, which is the significantly least amount among groups(p<0.05). LDL-C(101.3㎎/㎗) and triglycerides(135.5㎎/㎗) of activity group are the significantly least amount among groups, too(p<0.05). Amount of HDL-Cof activity group is greater than obesity, hypertension and diabetes group, but not significant. 3. Resting rate pressure product(RPP) is 6.60×10^3mmHg · bpm in activity group, which is less than those in obesity, hypertension and diabetes group(p<0.05). Values of hypertension group, 10.91×10^3mmHg · bpm, are the greatest among others(p<0.05). Maximal oxygen consumption(VO_2max) of activity group is 37.3me/kg/min, that is significantly greater than degerative groups(p<0.05). 4. Correlation coefficients with VO_2max have significance(p<0.001) ; TC(r=-0.853), HDL-C(r=0.633), LDL-C(r=-0.813), TG(r=-0.844), TC/HDL-C(r=-0.862), LDL-C/HDL-C(r=-0.834). Correlation coefficients with RPP have significance (p<0.001); TC(r=0.732), LDL-C(r=0.699), TG(r=0.705), TC/HDL-C(r=0.736), LDL-C/HDL-C(r=0.713).

렙틴 변형마우스에서 운동에 의한 간세포 자가포식 고찰

장준호, 강청훈, 임원청 청주대학교 보건의료과학연구소 2020 보건의료과학연구 Vol.8 No.2

Obesity is a multisystem disease caused by the failure of normal homeostatic mechanisms regulating food intake, fat storage, and energy utilization. Obesity causes the adverse effects in the body, such as inflammation, oxidative stress, and apoptosis. Leptin plays a clear role in obesity etiology, pathophysiology, and health outcomes, but it has not been fully understood. Leptin deficient ob/ob mice are a well-described model for metabolic studies that indicate hyperphagia and early onset obesity. Liver is a central organ with various functions including the elimination of toxins, the production of multiple hormones and proteins, and the storage of vitamins. This largest internal organ also plays a pivotal role in regulating blood sugar, and regulation of lipid storage. Autophagy plays a central role in energy homeostasis, and its induction is essential for lipolysis, a process that is also stimulated by leptin. The purpose of this study was to review the effect of exercise on the autophagy in the liver of leptin deficient ob/ob mice. This review also discuss how exercise in the liver of ob/ob mice activates autophagy and improves Non-alcoholic fatty liver.

Avenanthramide C suppresses hypoxia-induced cyclooxygenase-2 expression through sirtuin1 activation in non-small-cell lung cancer cells

임원청,강청훈 한국통합생물학회 2020 Animal cells and systems Vol.24 No.2

Avenanthramide C (AVC), found mainly in oats, mediates anti-inflammatory activities by reducing the anti-inflammatory cytokine levels. This study investigated the effects of AVC on hypoxiainduced cyclooxygenase-2 (COX-2) expression in A549 cells. AVC suppressed the hypoxiainduced increase in COX-2 protein levels and promoter activity. We also observed that the effects of AVC were reversed by a SIRT1 inhibitor, indicating that the inhibitory effects of AVC on hypoxia-induced COX-2 expression are mediated by SIRT1. Therefore, AVC inhibits the hypoxic induction of COX-2 expression via SIRT1 activation. Our results suggest that AVC could be beneficial for preventing lung inflammation under hypoxia.

8주간 하체운동이 여성노인의 신체조성과 SRT에 미치는 영향

정지훈,임원청,강청훈 한국스포츠학회 2019 한국스포츠학회지 Vol.17 No.1

본 연구는 8주간의 하체 운동이 여성 노인의 신체조성과 기대수명의 예측인자인 Sitting Rising Test (SRT)에 미치는 영향을 규명하는 것으로서 B 시 거주 65세 이상 여성 노인들을 연구 대상으로 하였다. 총 8주간 주3회에 걸쳐 각각 50분 동안 하체 저항성 운동프로그램을 실시 한 후 트레이닝에 따른 신체조성 (체중, 체지방률, 체지방량, 제지방 량)과 혈압 등의 인체 변인을 포함하여 근지구력 및 SRT 스코어의 변화를 측정하였다. 본 연구 프로그램을 통한 8주간 하체 저항성 운동은 체중과 체지방량을 유의하게 감소시켰으며, 근지구력 및 SRT 지표는 향상되는 것으로 나타났다. 이는 여성노인들의 하체운동을 통해 기대수명을 연장시킬 수 있음을 말해준다. In this study, we examined the effect of lower body exercise on body composition and sitting rising test (SRT) in elderly women. Subjects who were elderly women in B city participated in 8 weeks lower body exercise program. Sessions ran three times weekly for 50 min. Change of body composition, blood pressure, muscle endurance test and SRT were measured and statistically analyzed before and after the lower body exercise program. Lower body exercise decreased body weight and body fat mass, and increased muscle endurance and SRT score. Conclusionally, these data showed that lower body exercise in elderly women may extend life expectancy.

일회성 운동 중 초미세먼지(PM2.5) 과흡입이 마우스 폐의 염증 및 세포사멸 반응에 미치는 영향

염동진,Ziyi Zhang,박진한,장준호,소병훈,이강규,강청훈 한국운동생리학회 2023 운동과학 Vol.32 No.4

PURPOSE: The purpose of this study was to investigate the effects of hyperventilation in the presence of fine particulate matter (PM2.5) on inflammatory and apoptotic responses in the lungs of mice during a single bout of aerobic exercise. METHODS: The FVB/N mice were housed in a controlled SPF room and randomly divided into four groups: control (CON, n=7), fine particulate matter exposure (PM, n=7), PM and exercise (PME, n=7), and exercise only (EX, n=7). Acute PM exposure and/or aerobic treadmill exercise were undertaken according to the groups using a specially designed PM treadmill chamber. RESULTS: Protein expression and phosphorylation of p65 NF-kB in mouse lungs were significantly increased by acute exercise, but not by PM exposure. Basal protein levels and phosphorylation of MAPKs (p38, ERK, and JNK) were significantly increased in the PME group, but were lower in the PM and EX groups. Although the percentage of collagen fibers in lung tissue was not affected by PM exposure, the expression of pro- and antiapoptotic factors BAX, BID, and Bcl-2 was significantly elevated by inhalation of PM during exercise. CONCLUSIONS: In this study, the MAPKs family was activated by excessive PM inhalation during exercise and similar changes in apoptosis-related signaling were observed. This suggests that even a single bout of inhalation of fine particulate matter during exercise can affect factors associated with inflammation and cell death.

운동 중 미세먼지 과흡입 생체 실험모델 고안

장준호 ( Junho Jang ),임원청 ( Chounghun Kang ),강청훈 ( Wonchung Lim ) 중앙대학교 학교체육연구소 2020 Asian Journal of Physical Education of Sport Scien Vol.8 No.3

최근 들어 국내 대기오염 증가에 따른 사회·경제적 손실이 증가되고 있으며, 그중 특히 미세먼지는 국내 오염원 배출뿐만 아니라 주변국의 영향으로 그 심각성이 점점 증가하고 있다. 대기 중 미세먼지 발생으로 인해 생활체육, 레저 활동 등이 제한되며, 청소년기 학생들에 있어서는 야외 신체활동 및 학교 체육수업의 위축을 초래하고 있다. 하지만 미세먼지 흡입에 따른 다각적인 생체 영향 평가가 요구됨에도 불구하고 특히, 운동 중 미세먼지 과흡입에 따른 실험연구는 현재 검증된 실험 장비 및 모델이 없어 현실적으로 이루어지지 못하고 있는 실정이다. 본 연구를 통해 운동 중 미세먼지 과흡입으로 인한 마우스 in vivo 실험모델(미세먼지 운동 챔버)을 최초 구축하였으며 이를 마우스 모델을 이용하여 검증하였다. 또한, 운동 중 미세먼지 노출에 따른 마우스의 뇌에서 미토콘드리아 자가포식에 미치는 영향을 mt-Keima 마우스를 이용하여 검증하였다. 그 결과, 미세먼지 챔버 내 운동 프로그램 실험 모델의 유효성을 확인할 수 있었으며, 이를 활용하여 향후 다양한 조건에서의 미세먼지 생체 바이오마커 발굴의 가능성을 확보하였다. 향후 본 연구 장비를 활용한 생체 바이오마커의 발굴은 임상 연구로의 연계 적용이 가능해지며, 이는 미세먼지 농도에 따른 학교체육 시행 기준에 대한 과학적 근거를 제시하는 등 활용가치가 높을 것으로 사료된다. Recently, due to the increase in domestic air pollution, socio-economic losses are increasing, and among them, not only domestic pollution but also the influence of neighboring countries seem to be making the problems of particulate matters(PM) more worsen. The negative effect on the human body due to the increase in the concentration of PM causes limitations in physical activities, including sports and physical education classes. Despite a multifaceted study of the pathology caused by inhalation of fine dust, studies on risk assessment for over-inhalation of fine dust during exercise are lack due to the absence of proper experimental equipment. Thus, this study was designed to establish in vivo exercise program in the PM chamber and verify the systems by testing the effect of PM during exercise on mitochondrial homeostasis in the mouse brain. This study confirmed the feasibility of the experimental model of the exercise intervention in the PM chamber developed in this study for the first time and it would be excepted to provide guidelines for physical education classes in relate to the PM in school in the future.

단기간 트레드밀 운동을 통한 in vivo 미토파지 실험모델 검증

김동현 ( Donghyun Kim ),소병훈 ( Byunghun So ),박진한 ( Jinhan Park ),강청훈 ( Chounghun Kang ) 한국체육학회 2021 한국체육학회지 Vol.60 No.4

미토콘드리아는 에너지공급 및 다양한 신호전달을 통해 세포의 항상성에 관여한다. 미토콘드리아의 손상은 만성질환과 밀접하게 관련되어 있으며, 질병 치료 및 예방목적으로 활발하게 연구되고 있다. 미토파지(mitophagy)는 손상된 미토콘드리아를 선택적으로 제거하여 세포기능의 유지 및 개선에 중요한 역할을 담당하는 세포 작용이다. 규칙적인 운동은 손상된 미토콘드리아를 제거하여 미토콘드리아 질적 조절을 통해 생리적 대사 및 기능을 제어하는 것으로 알려져 있다. 지금까지 미토파지 평가에 대한 연구방법은 많이 발전되어 왔지만, 미토파지 관련 현상의 in vivo 검증은 부족하다. 따라서 본 연구는 1주간의 마우스 트레드밀 운동이 골격근의 선택적 미토콘드리아 제거 신호에 미치는 영향을 mt-Kiema 마우스를 통해 분석하였다. 그 결과, 운동(EX)군이 대조(CON)군에 비해 더 높은 미토파지(red 형광) 수준을 나타냈으며, 이는 미토파지-관련 단백질 발현 결과와 유사성을 보였다. 본 연구는 in vivo 미토파지 평가 모델을 통해 미토콘드리아 질적조절에 대한 운동중재 효과를 보다 정확하게 검증할 수 있는 방법으로, 향후 본 연구모델의 활용가치가 높을 것으로 사료된다. Mitochondria are intracellular organelles, involve in regulating cellular homeostasis through various signaling pathways. Damaged mitochondria are closely related to chronic diseases, and have been actively studied for the patho-physiologic conditions. Mitophagy is a cellular mechanism of action that plays an important role in maintaining and improving cellular functions by selectively removing damaged mitochondria and exercise regimen is known to regulate mitochondrial quality control including the process of mitophagy. In this study, the effect of treadmill exercise for 1 week on the in vivo mitophagy of skeletal muscle was analyzed using mt-keima transgenic mice. As a result, the higher red fluorescence (pH 4.0; excitation: 561nm, emission range: 570-695nm) ratio was observed on confocal microscopy in the exercise (EX) group vs. the control (CON) group. These results were also consistent with the data that observed protein levels which are known to be induced during mitophagy. Therefore, the effect of exercise intervention on mitochondrial quality control can be more accurately verified through validation using the in vivo mitophagy evaluation model, mt-Kiema mice.

장내 미생물의 비만 및 운동 효과에 대한 통찰

박진한 ( Jinhan Park ),김동현 ( Donghyun Kim ),장준호 ( Junho Jang ),강청훈 ( Chounghun Kang ) 중앙대학교 학교체육연구소 2021 Asian Journal of Physical Education of Sport Scien Vol.9 No.4

수많은 대사 질환의 원인은 인간의 게놈, 식습관 및 신체 활동의 변화가 핵심 요소로 인식되고 있었다. 하지만 지난 10여년 동안 인간의 미생물군이 체내에 공존하여 다양한 질병에 영향을 미친다는 것이 밝혀지는 등 마이크 로바이옴 프로젝트 이후 장내 미생물이 다양한 환경에서 체중과 에너지 대사를 조절하는 것으로 연구되었다. 비만군과 정상군을 비교하였을 때, 장내 미생물의 구성의 차이가 나타났으며 장내 미생물의 역할에 따라 에너지 생산과 저장을 조절하여 체중의 증가 및 지방의 축적에 관여를 하는 것으로 입증되었다. 또한, 비만을 개선하기 위한 운동은 마이크로바이옴의 구성을 다양화하고 체내 조직에 에너지를 공급하여 운동 능력을 변화시킬 수 있는 가능성을 보여주었다. 예를 들어, 비만으로 인하여 락토박실리우스(Lactobacillus), 베일로넬라(Veillonella) 등의 후벽균(Firmicutes)의 군집이 풍부해졌고 Lactobacillus, 베일로넬라(Veillonella)같은 의간균(Bacteroidetes)의 군집이 감소하나 운동을 통하여 정상 수준의 미생물 군집 균형으로 회복되는 것으로 나타났다. 따라서, 마이크로바이옴은 운동을 통한 장내 미생물 군집의 변화를 통하여 비만을 치료하거나 예방하는데 있어 새로운 접근방법으로 그 역할이 기대된다고 할 수 있다. 본 연구는 현재까지의 대사 질환 및 운동에 의한 장내미생물의 영향에 대한 연구 결과를 분석하고 그에 따른 대사질환 개선 혹은 운동 경기력 증진에 대한 가능성을 총설한다. In the late 2000s, it was discovered that the human microbiome coexists in the body and affects a variety of diseases. In many studies, changes in the human genome,diet, and physical activity are the causes of a variety of diseases. However, after the entire human microbiome project, various microbial studies have been conducted to suggest that intestinal microbes are a key factor in regulating body weight and energy metabolism. The composition of the microbial group showed a difference when comparing the obese and the normal groups. In addition, exercise, known as a treatment for obesity, has been shown to diversify the composition of microbial communities and supply energy to tissues. This, it raises the possibility that microbiome can be a useful tool in treating or preventing obesity through changes in the overall gut microbiome through exercise, that can be a new means of treating obese patients and enhancing exercise performance.

응급처치교육 이수여부에 따른 응급처치 지식수준 및 인식

김형근(Kim, Hyeong-Keun),임원청(Lim, Won-Chung),강청훈(Kang, Choung-Hun) 한국체육과학회 2019 한국체육과학회지 Vol.28 No.6

The purpose of this study is to prevent safety accidents and to help development of first aid education programs in case of safety accidents of modern people where exposure frequency of various safety accidents as well as the level of danger increases. To achieve the purpose of this study, 472 university and graduate students in the metropolitan area were selected by convenience sampling method. 472 questionnaires were circulated and collected and analyzed 408 questionnaires except for 64 questionnaires which were deemed unreliable. The conclusion of the study is as follows. When it comes to the level of first aid knowledge depending on completion of the first aid training, trained people showed higher knowledge level than untrained. When it comes to the perception on first aid education depending on first aid training, both trained and untrained people showed high perception level in first aid education. The average level of first aid knowledge depending on the number of first aid training was higher as the number of education increased. Therefore, based on the high level of perception in first aid education, first aid education should be repeatedly implemented at each regional unit, and efforts should be made at the same time to prevent the theoretical education and to transform the training into the practical training for individual emergency situation.