Cyclic Nona 및 Pentapeptide 유도체들의 합성과 단백질 분해 효소에 대한 저해활성 측정

강신원,허남원,신홍대 ( Shin Won Kang,Nam Won Huh,Hong Dae Shin ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.4

Most of legume seeds contain serveral kinds of BBI-type inhibitors. They have similar amino acid residues around the reactive site. The P₁ site amino acid residue in reactive site generally corresponds to the specificity of the cognate enzyme. It has also been noted the importance of P₁ site amino acid residue in synthetic cyclic nonapeptide derivatives which were related to active site of BBI. To obtain further understanding of the role of amino acid residues near th reactive site, 3 cyclic nonapeptides which are substituted analogues of GBI-II were synthesized and the inhibition constants for some proteinases were calculated by Dixon method. Antielastic fragment in GBI-II (I_a) differ only P₂ Site with that of soybean isoinhibitor C-II, Ki of Ia for porcine pancreatic elastase was 48μM, Ki of antitryptic fragment in LBI (I_b) for trypsin was 3.7μM, and Tyr analogue (I_c) for chymotrypsin showed no inhibition but appeared low inhibitory activity for trypsin and elastase. To elucidate the relation between loop size and inhibitory activity, 3 cyclic pentapeptide analogues containing the each reactive site of Ala-Ser, Lys-Ser and Tyr-Ser were synthesized. These peptides possessed slight inhibitory activity toward proteinases, Ki of synthetic cyclic pentapeptides for elastase, trypsin, and chymotrypsin were 614μM, 278μM and 128μM, respectively.

역사(歷史) 사회(社會) 환경(環境)과 구장산술(九章算術의) 구조(構造)

강신원,Kang, Shin-Won 한국수학사학회 2006 Journal for history of mathematics Vol.19 No.4

중국(中國)의 산경(算經)에서 제일(第一) 먼저 우리의 관심(關心)을 끄는 것은 구장산술(九章算術)이다. 그러나 이천삼백년(二千三百年) 전(前)부터 존재(存在)하였다고 추측(推測)할 뿐 편저자(編著者)나 제작년도(製作年度)는 미상(未詳)이다. 여기에 있는 문제(問題)들을 수학적(數學的)인 명(面)에서 검토(檢討)하여 보는 것도 바람직하지만 역사(歷史)나 사회적(社會的) 환경(環境)과 관련(聯關)시켜 분석(分析)하고 추론(推論)하여 구장산술(九章算術)의 구조(構造)를 밝혀낸다. The Nine Chapters on the Mathematical Art has dominated the history of Chinese mathematics. It served as a textbook not only in China but also in the neighbouring countries and regions. The book is anonymous like many Chinese classics. The Nine Chapters contains 246 problems and their solutions, some of which date back to before the Qin Dynasty $(221\sim207\;B.C)$ and it seems to have been written by various writers over many generations. In this paper, we will investigate the structure of the Nine Chapters from the view points of ancient social environments which entail eventually mathematics in the Nine Chapters.

Cyclic Nona 및 Pentapetide 유도체들의 합성과 단백질 분해 효소에 대한 저해활성 측정

강신원,허남원,신홍대,Kang, Shin-Won,Huh, Nam-Won,Shin, Hong-Dae 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.4

콩과식물의 씨속에 포함된 각종 BBI형 저해물질들은 reactive site를 중심으로 유사한 아미노산 잔기를 가지고 있다. 일반적으로 reactive site 내의 $P_1$ site 아미노산 잔기는 상대효소에 대한 특이성이 있다. Reactive site 뿐만 아니라 그 주변에 위치한 아미노산 잔기의 역할을 알아보기 위해 GBI-II의 antielastic fragment를 모델로 하여 $P_1$ site 아미노산 잔기를 치환시킨 3종의 cyclic nonapeptide를 액상법으로 합성하고 몇 종의 단백질 분해효소에 대한 $K_i$값을 Dixon 법으로 계산했다. GEI-II의 antielastic fragment $(I_a)$는 porcine pancreatIc 에라스타제에 대하여 $48{\mu}M$의 $K_i$값이 나타났고 LBI의 antitryptic fragment $(I_b)$는 트립신에 대한 $K_i$값이 $3.7{\mu}M$이었으며 Tyr 유도체인 $I_c$는 키모트립신에 대하여 저해활성이 없었지만 트립신과 에라스타제에 대하여 낮은 저해활성을 보였다. Loop size와 저해활성간의 관계를 알아보기 위해 $P_1$ site를 각각 Ala ($I_a$'), Lys ($I_b$') 및 Tyr ($I_c$')으로 치환시킨 3종의 cyclic pentapeptide를 합성하여 저해활성을 구한 결과 $I_a$'은 에라스타제에 대하여$614{\mu}M$, $I_b$'은 트립신에 대하여 $278{\mu}M$ 또한 $I_c$'은 키모트립신에 $128{\mu}M$의 $K_i$값으로 나타났다. Most of legume seeds contain serveral kinds of BBI-type inhibitors. They have similar amino acid residues around the reactive site. The $P_1$ site amino acid residue in reactive site generally corresponds to the specificity of the cognate enzyme. It has also been noted the importance of $P_1$ site amino acid residue in synthetic cyclic nonapeptide derivatives which were related to active site of BBI. To obtain further understanding of the role of amino acid residues near th reactive site, 3 cyclic nonapeptides which are substituted analogues of GBI-II were synthesized and the inhibition constants for some proteinases were calculated by Dixon method. Antielastic fragment in GBI-II $(I_a)$ differ only $P_2$' Site with that of soybean isoinhibitor C-II, Ki of Ia for porcine pancreatic elastase was $48{\mu}M$, Ki of antitryptic fragment in LBI $(I_b)$ for trypsin was $3.7{\mu}M$ and Tyr analogue $(I_c)$ for chymotrypsin showed no inhibition but appeared low inhibitory activity for trypsin and elastase. To elucidate the relation between loop size and inhibitory activity, 3 cyclic pentapeptide analogues containing the each reactive site of Ala-Ser, Lys-Ser and Tyr-Ser were synthesized. These peptides possessed slight inhibitory activity toward proteinases, Ki of synthetic cyclic pentapeptides for elastase, trypsin, and chymotrypsin were $614{\mu}M$, $278{\mu}M$ and $128{\mu}M$, respectively.

FTA 통신서비스 상호접속협정 공개의 주요이슈 연구

강신원(Shin-Won Kang),배홍균(Hong-Kyun Bae) 국제e-비즈니스학회 2012 e-비즈니스 연구 Vol.13 No.1

통신사업자 합병과 소비자 편익 -통신서비스요금을 중심으로-

강신원 ( Shin Won Kang ),조상섭 ( Snag Sup Cho ) 한남대학교 과학기술법연구소 2009 과학기술법연구 Vol.15 No.2

The telecom operator merger affects in consumers a lot of important terms rather than a simple merger between two companies. In this study, the impacts of KT-KTF merger in consumer, the greatest issue in the telecommunication market in 2009, was analyzed. To analyze the consumer benefits from the merger, first of all, merger-related issues were derived. And also merger-related theory which is based on consumer benefits was reviewed. In addition, the policy implications for expanding the consumer benefits by analyzing the merger of major countries and practice were derived. In accordance with the merger of large telecom operators, the great social and economic impact is expected. However, in this study, we reviewed the consumer benefits in accordance with telecom carrier merger, the study was mainly focused on telecommunication services tariff as the consumer benefits.

스마트폰 관련 규제정책이슈 연구

강신원 ( Shin Won Kang ),조상섭 ( Sang Sup Cho ) 한남대학교 과학기술법연구원 2011 과학기술법연구 Vol.17 No.1

Smartphone-related regulation affects significantly on its activation as telecommunication industry and services. From this perspective, this study mainly focused on issues of Smartphone-related law and regulation. Thus, a regulatory policy issue of smartphone, as internet real name system, game pre-examining system, location-based service, mobile payment, were studied intensively. In conclusion, smartphones enabling policies will increase demand for related industry and services. In addition, it will play a major role to continue the secure national competitiveness through by maintained the image of IT powerhouse and increasing the industrial efficiency and personal satisfaction. Thus, further research, the ongoing policy issues related to smart phones, is required.

자유무역협정(FTA)에 따른 국내통신시장 법,규제 환경변화 연구

강신원 ( Shin Won Kang ),조상섭 ( Sang Sup Cho ) 한남대학교 과학기술법연구소 2010 과학기술법연구 Vol.16 No.2

In this paper, we have investigated the changes in the laws and regulatory environment in domestic telecom market due to FTA. For this, first of all, we figured out the Korea-U.S and Korea-EU FTA which are expected to affect the most important influence to Korean Market. The Korea-US FTA was signed ahead than Korea-EU FTA. However, Korea-EU FTA is similar to Korea-US FTA. Other FTA, lower openness level than Korea-US FTA, were excluded from analysis. For this reason, our study were focused on Korea-US and Korea-EU FTA. Based on Korea-US and Korea-EU FTA, we were discussed the telecom services regulation, foreign ownership restrictions in basic telecommunication operators, technology standards policy, cross-border supply(or trade), interconnection, etc. In present and in near future, the issues of the cross-border supply(or trade), foreign ownership restrictions in basic telecommunication operators and interconnection are expected to affect a lot in the domestic Laws and regulations. Therefore, the implications were drawn from these three kinds of important issues.

통신서비스 요금 및 정산에 관한 국제표준화 방향

강신원(Kang, Shin-Won),박혜진(Hye Jin PARK) 한국통신학회 2007 한국통신학회 학술대회논문집 Vol.2007 No.7

주요국의 통신시장 경쟁상황평가 법,제도 검토

강신원 ( Shin Won Kang ) 한남대학교 과학기술법연구소 2009 과학기술법연구 Vol.14 No.1

In July 2007, Korea established new market review regulations. The regulations will great influence on competition enlargement and competition structure on telecommunication market. To get effective results of market review, rationality, predictability, and flexibility of policy required. In addition, proactively, market review need to apply based on reasonable competing philosophies and competition law. Therefore, in this paper, we study market review of some major countries, review focused on law and systems. In conclusion, Korea`s market review regulations are as good as some major countries such as U.S, U.K, Australia, and EU as theoretical aspects of market review indicators or definition of unit market. On the other hand, results show that public consultation system for ensuring transparency and objectivity are very weak.

WTO DDA 통신시장 자유화협상에 대한 주요 이슈 고찰

강신원(Kang Shin-Won) 한국무역학회 2003 한국무역학회 세미나 및 토론회 Vol.2003 No.2