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Streptozotocin 투여 용량에 따른 당뇨병성 마우스의 신경전도도 변화에 대한 연구
강동호(Tong Ho Kang) The Research and Information Service 2006 남부대학교 논문집 Vol.6 No.-
본 연구는 streptozotocin 유발 type 1 당뇨병 mouse 동물 모델에서 당뇨병성 신경병증과 streptozotocin 용량과의 상관관계를 규명하고자 하였다. 구체적으로 streptozotocin의 용량을 50mg/kg, 100mg/kg, 150mg/kg 으로 투여하여 mouse에서 당뇨병을 유발하였고, 31일 경과 후 mouse 좌골신경에서 운동신 경 및 감각신경의 신경전달 속도를 측정 하여 당뇨병성 말초신경병증의 정도를 평가하였다. Streptozotocin 투여용량별 체중변화는 streptozotocin을 투여하지 않은 그룹과 비교하여 streptozotocin을 투여한 그룹들의 체중이 감소했고 streptozotocin의 용량증가에 따라 체중 감소율은 유의적으로 증가하였다. Streptozotocin 투여용량에 따른 평사 (non fasting)와 공복시(fasting) 의 혈당은 streptozotocin의 투여용량이 증가할수록 혈당은 증가하는 것으로 나타났다. Streptozotocin 투여용량에 따른 신경전달속도 변화를 평가 사streptozotocin 투여 31일 경과시 감각신경의 신경 전달속도는 의미 있는 차이가 나타나지 않았으나 운동신경의 신경전달속도는 streptozotocin올 투여한 그룹에서 유의미하게 감소를 나타냈고 streptozotocin 용량의존적으로 감소하는 경향을 보여주었다. 따라서 운동신경 및 감각신경 양자 모두에서 신경전달속도의 뚜렷한 감소를 확인하기 위해서 는 streptozotocin 투여 후 고혈당증의 지속기간이 좀 더 필요한 것으로 사료된다.
산약의 항당뇨 특성 연구(2) -NGF 유도 신경병증예방-
강동호 ( Tong Ho Kang ),최상진 ( Sang Zin Choi ),이태호 ( Tae Ho Lee ),손미원 ( Mi Won Son ),박지호 ( Ji Ho Park ),김선여 ( Sun Yeou Kim ) 한국식품영양학회 2008 韓國食品營養學會誌 Vol.21 No.4
The main cause of diabetic neuropathy, one of the most debilitating complications, is the chronic hyperglycemia, the increase sorbitol or the decrease of nerve growth factor(NGF). NGF, a protein, plays a major role in the development and maintenance of peripheral nervous system. Systemic administration of NGF prevents manifestations of neuropathy in rodent models of diabetic neuropathy. In the previous investigation, we report the hypoglycemia effect of Dioscorea rhizoma extract(DRE) in diabetic mice. The present study shows protective effect of DRE on diabetic neuropathy by induction of NGF protein. We investigated the NGF level in salivary gland and sciatic nerve of normal mouse and the effect of DRE on sciatic nerve conductivity and thermal hyperalgesia test in Type 2 db/db mouse. DRE increased endogenous NGF level in salivary gland and sciatic nerve of mouse. And sensory nerve conductivity velocity(SNCV), motor nerve conductivity velocity(MNCV) and thermal hyperalgesia increased in DRE treatment mice compared with control group. On the basis of our results, we conclude that DRE increase induction of endogenous NGF level and have protective effect on diabetic neuropathy by induction of NGF. Therefore, we propose that long-term use of DRE might help prevention of diabetes-associated complication; diabetic neuropathy.
설문지를 통한 고등학생들의 MP3 사용 실태 및 청력에 미치는 영향
홍하나,강동호,홍빛나,Hong, Ha Na,Kang, Tong Ho,Hong, Bin Na 한국음향학회 2013 韓國音響學會誌 Vol.32 No.1
This study surveyed MP3 player usage and evaluated its effect on hearing thresholds in high school students. It is reported an increase in risk factors for hearing loss in adolescents that paralleled the increase in sales of MP3 players. A total of 538 students in two high schools in Gwangju province participated in this study. The students completed questionnaires about their MP3 player use and their pure tone hearing thresholds were tested at several frequencies. The questionnaire results indicated that 61.1%, of the students listened to music on MP3 players for more than one hour per day, 71.6% listened for four or more days per week, and 36.7% had listened for more than 3 years. Listening at a high volume of 60% of the maximum level was reported by 51% and they recognized having ear pains. The pure tone hearing threshold was found to be raised for the adolescents who had a long history of MP3 listening at high volume (p< 0.0001). 본 논문에서는 청소년들의 MP3 사용 실태와 그에 따른 청력역치를 평가했다. 청소년들의 MP3 사용률은 지속적으로 증가하고 있으며 청력과 관련하여 위험요소가 되고 있다. 본 연구에서는 광주 지역의 2개 고등학교에 재학 중인 남녀 538명을 대상으로 MP3의 사용 실태를 조사하고 청력 역치를 측정하여 사용 유형별로 상관성을 평가했다. 분석 결과, 하루중 1시간 이상 MP3 사용률이 61.1%였으며, 주당 4일 이상 사용하는 비율이 71.6%이고, 3년 이상 사용한 경우가 36.7%로 나타났다. 조사대상의 51%가 볼륨 크기를 60%이상으로 듣고 있으며 귀 통증을 인식하고 있었다. MP3를 장시간 사용하며 소리를 크게 듣는 청소년과 그렇지 않은 청소년들의 청력은 유의한 차이가 있는 것으로 나타났다 (p< 0.0001).
부영민 ( Yung Min Bu ),백선경 ( Sun Kyung Baek ),김진화 ( Zhen Hua Jin ),강동호 ( Tong Ho Kang ),노승주 ( Seung Ju Rho ),김호철 ( Ho Cheol Kim ) 대한본초학회 2004 大韓本草學會誌 Vol.19 No.2
N/A Objectives: The root of Rhodiola sachalinensis A. bor (RS), which has been used to strengthen qi(補氣), reduce heat of lung (淸縮), nourish heart (養心), stop bleeding (止血), reduce edema(消腫) for the treatment of fatigue, amnesia, bleeding and edema. It`s flavour and channel tropism is sweet (甘), acting on the lung (肺). The purpose of the study we report here was to determine the neuroprotective effect of the roots of RS on transient focal cerebral ischemia in SD rats. Methods: The root of RS was lyophilized after extraction with 85% methanol. We occluded middle cerebral artery for 90 minutes and reperfused for 24 hours. The brain slices were stained by 2 % 2, 3, 5-triphenyltetrazolium chloride and the infarct volume was measured by graphic analyzer. Results: The root extracts of RS showed significant neuroprotective effects (100 mg/kg, p〈0.05) compared with control group. The neuroprotective efficacy was about 35%. Immunohistochemical data explained the protective effects of RS via attenuation of COX-2 induction in neocortex and inhibition of microglia activation in the penumbral region. Conclusions: The root of RS has neuroprotective effects on transient focal cerebral ischemia in Spargue-Dawley rats and the mechanism might be the attenuation of COX-2 induction and microglial activation.
오디 Flavonoid의 alloxan 처리 zebrafish 췌장섬에 대한 회복효과
서경화 ( Kyeong Hwa Seo ),남윤희 ( Youn Hee Nam ),김영언 ( Young Eon Kim ),홍억기 ( Eock Kee Hong ),홍빛나 ( Bin Na Hong ),강동호 ( Tong Ho Kang ),백남인 ( Nam In Baek ) 한국응용생명화학회(구 한국농화학회) 2015 Journal of Applied Biological Chemistry (J. Appl. Vol.58 No.1
오디에서 분리한 flavonoid가 zebrafish에 alloxan을 처리하여 손상된 췌장섬을 어느 정도 회복 시키는지 검토하였다. Zebrafish의 배아에 alloxan을 처리하면 확실하게 췌장섬의 크기가 감소하였다(p <0.001). Rutin, isoquercetin 및 quercetin의 처리에 의해 췌장섬의 크기가 유의적으로 증가하였으며(p <0.001), 형광세포의 fluorescence intensity도 뚜렷하게 증가하였다. Flavonoids from Morus alba fruits were evaluated for recovery effect on the damage of alloxan-induced pancreatic islet in zebrafish. Alloxan treatment on the zebrafish embryo surely decreased the pancreatic islet size (p <0.001). Rutin, isoquercetin, and quercetin very significantly recovered the size (p <0.001) and the fluorescence intensity of pancreatic islet.
HPLC용 형광유도체화제인 1-(N,N-dimethylamino)pyrene-6-sulfonyl chloride를 이용한 GABA와 Glutamate의 HPLC분석에 관한 연구
이윤중,김용희,강동호,박귀례 성균관대학교 약학연구소 1991 成均藥硏論文集 Vol.3 No.1
Abstract-Fluorescent derivatives of amino acid neurotransmitter such as GABA and glutamate were synthesized using 1-(N,N-dimethylamino)pyrene-6-sulfonyl chloride (DAPS-Cl) as a derivatizing reagent. GABA and glutamate were derivatized quantitatively into fluorescent compounds by reacting with DAPS-Cl. The optimal conditions for the derivatization of GABA and glutamate with DAPS-Cl were estimated such as pH, reaction temperature and time, and the amount of DAPS-Cl. The derivatives were separated on a reverse phase column with a gradient elution using the 30 mM phosphate buffer and acetonitrile, and monitored by fluorescence detector. Calibration curves for GABA and glutamate exhibited good linearty over 1∼5 pmol. GABA and glutamate in hippocampus of rat brain were determined. After postmortem of rat brain, GABA increasing and glutamate decreasing in hippocampus were prevented 3-mercaptopropionic acid and microwave application.