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      • 人血淸의 C. Albicam에 對한 抗菌性物質에 對해서

        鄭在奎,徐桂益,張炳榮,鄭純鎔 최신의학사 1969 最新醫學 Vol.12 No.2

        Pooled healthy human sera exhibited a marked growth inhibitary action in vitro against strains of C. albicans and this action was stronger than that of pooled rabbit sera. Growth inhibitory substance to C. albicans which is contained in serum has not been absorbed with zymosan or digested by trypsin, but destroyed by ox bile or sodium desoxychoiate, this illustrats that the antibacterial substance contained in serum is not exactly the same one as that of anticandidial substance.

      • 綠膿菌의 Oxidase 反應에 對하여

        鄭在奎,朴商煥,金正壽 慶北大學校 醫科大學 1961 慶北醫大誌 Vol.3 No.2

        Pseudomonas species produce an abundant quantity of cytochrome and cytochrome oxidase and is utilized for the identification of this species from clinical specimen, Gaby, especially emphasized the use of p-amino-dimethyl aniline oxalate for the oxidase test of Ps. aeruginosa. In connection with aerobic respiration, catalase and perioxidase are also usually found in aerobic bacteria. In the present study, author examined toe enzyme catalase, perioxidase and cytochrome oxidase of pseudomonas sp. isolated from surgical wounds to investigate the relationships and the value of these tests. 1) Pseudomonas. sp. show somewhat marked catalase and perioxidase activity as comp are to the other enteric bacilli, however the detection of these enzyme have no diagnostic value. 2) Pseudomonas sp. form a strong indophenol oxidase and this was specific as compare to enteric forms, with a diagnostic value, Gaby reagent, Kovac's reagent, and Nadi reagent are sensitive in same degree. 3) The enzyme as well as pyocyanin formation are weakened by the transfer in the medium containing glucose or cystine.

      • Staphylococcus epidermidis의 성상과 Plasmid profile의 상관에 대해서

        정재규,김성광,박미경,김희선 대한화학요법학회 1986 대한화학요법학회지 Vol.4 No.1

        안결막 및 그 외 병소로부터 분리한 23주의 coagulase 음성 포도구균을 분류하고 항생제에 대한 감수성검사와 각균주의 plasmid분포의 검사를 통하여 EtBr처리에 의한 약제내성의 cure와 plasmid소실과의 상관성을 관찰하였다. penicillin G와 cephalothin에 대해서는 10%내외, ampicillin, noboviocin, erythromycin, tobramycin, gentamycin, kanamycin 및 amikacin에 대해서는 20~30%, 그리고, tetracycline 및 chloramphenicol에 대해서는 각 47.8%와 56.3%의 균주가 내성을 나타내었다. 공시균주 중 17주(73.9%)가 plasmid를 보유하고 있었고 tetrcycline내성주(다제내성주 포함) 11주는 2.9 Mdal의 plasmid를 가진 6주와 이보다 작은 plasmid를 가진 4주로 나누어졌으며, 후자의 4주 중에서는 tetracycline cured strains만 얻을 수 있었고 이들 Tccured strain들 중에는 plasmid소실이 수반되는 균주도 있었다. 2.9Mdal의 plasmid를 가지는 S_16주에서 얻은 cured strain들에서는 tetracycline cured strain들만 plasmid band가 소실되었다. Coagulase negative Staphylococci isolated from conjunctiva and other sources were classified and observed the relationship between antibiotics resistance patterns and plasmids profiles of these isolates, also antibiotics curing experiments were carried out with ethidium bromide in accompanying with the loss of plasmids. Most isolates belonged to Staphylococcus epidermidis(69.6%) and cephalothin was the most sensitive antibiotics(8.7%) next to penicillin, and chloramphenicol was the most resistant drug(56.5%) next to tetracycline. Seventeen strains harbored plasmids, and eleven tetracycline resistant strains divided into two groups by molecular size of plasmids, i.e. 6 strains of 2.9Mdal and 4 strains of less than this size. From 4 strains of latter group, only tetracycline cured strains were isolated with or without loss of plasmid, however, from one strain of former group, tetracycline, chloramphenicol and erythromycin cured strains in single or in combination with were isolated but only tetracycline cured strains were accompanied with the loss of plasmids compared to parent strain.

      • 結核菌의 抗結核劑에 對한 抵抗性 : 第一報 分離菌의 抗結核劑에 對한 耐性

        鄭在奎 慶北大學校 醫科大學 1958 慶北醫大誌 Vol.1 No.1

        Sensitivity test was performed on various sources of samples from tuberculous patients, by the modification of Ogawa's indirect, direct and prompt methods. For the purpose of detecting the colonial difference of resistancy, 15 cases were examined by picking up 4 colonies from each culture, and subcultured on a series of drug containing media. A survey of 225 tests was carried out in 112 patients and the following results were obtained. ⑴ 53 cases were resistant against streptomycin (47.3%). Three ceses out of them were those who had never received any streptomycin therapy. ⑵ 12 cases were resistant against I.N.A.H. (14.4%) and 8 cases were resistant against P.A.S. (8.4%) among 83 cases. ⑶ No marked colonial difference of resistancy was observed. However, slight difference was detected in 5 cases out of 15.

      • 마늘의 Candida sp. 에 對한 抗菌作用에 關해서

        鄭在奎,金渭祥,朴佑淳,鄭弼壽 慶北大學校 1962 論文集 Vol.5 No.-

        In the previous communication, the growth inhibitory action of garlic on C. albicans was reported and discussed about the candida distribution of Korean people. In the present study, the inhibitory and lethal effects of garlic on candida sp. were studied further detail and the resistance of oral candida sp. isolated from various age groups, to garlic was examined. (1) The growth inhibitory and lethal concentration of garlic juice on standard candida sp. were differ according to the species, however 0.1-0.2% concentraion of garlic juice in Sabouraud media inhibit the growth and 0.6-1.2% concertration of garlic juice exhibit a lethal effect on cardida sp. within 12 hours. (2) The resistance formation or a difference of resistance level of the isolated strain from various age group were not found and candida sp. seems to be not form resistance easily against garlic.

      • 紫外線으로 誘發시킨 C. Albicans의 變異性

        鄭在奎 慶北大學校 1958 論文集 Vol.3 No.-

        The suspension of C. abicans was irradiated under the sublethal dose of ultra-violet light and subcultured on a teterazolium chloride Sabouraud media to detect the tetrazolium reduction. In the course of irradiation, a few white colonies were noted which were markedly differing in growth rate and pigment reduction as compared with the normal colony. These were refered as W-colony arbitrarily in this report to distinguish these from the normal red colony (R Colony). Various characteristics of teh W and R colony are as follows: (1) No morphological differences such as pseudomycelium formation and Chlomydospore formation between them were noted. However, the growth rate of the was form was significantly slower than the R form. (2) There was no difference in sugar fermentation between R and W forms except for the W form are somehow slower as compared with the R form. (3) There was no difference in tetrezolium reduction between R and W forms substantially, when the examination was performed with a cell suspension, but on the tetrazolium solid Sabouroud media, the W forms formed white small colonies as compared with the R colonies. (4) The W-form showed markedly reduced toxicity in mice.

      • 口腔內 Candida Sp. 分布에 對하여

        鄭在奎 慶北大學校 1958 論文集 Vol.3 No.-

        Candida is normally found in the healthy oral cavity and rarely reveals its pathogenicity in a form of local or general infection in the state of reduced resistance-of the host. It is well known that the incidence of infection has inreased markedly since the advent of antibiotic therapy, and moniliasis is now becoming a serious problem. The distribution of candida population in the mouth of korean was surveyed in infants, healthy college students and University Hospital patients who have received various kinds of antibiotics therapy indiscriminately. Specimen was directly taken with-cotton swab in all groups and innoculated immediatly on Sabouraud media. In the student group, both swab and gougling methods of sampling were used to compare the positive incidences in the two methods. The percentage of positive-incidences are as follows: (1) Infant group-22.6% (2) Patient group-21.8% (3) Student group (swab)-6.6% (gougling)-11.7% The incidence of candida positive case in this series is lower-than that reported by Young. The relationship between food elements, particular to Korean, such as garlic, pepper, and the concentration of salt was studied. The growh of C. Albicans was markedly inhibited by garlic in low concentraion, and also in high concentration of salt.

      • Chioromycetin이 typhoid 抗體形成에 미치는 影響

        鄭在奎 慶北大學校 醫科大學 1958 慶北醫大誌 Vol.1 No.1

        The use of various kinds of antibiotics made the symptoms and prognosis of diseases different from the time when they were not in use. In case of the enteric infections such as typhoid or paratyphoid, the clinical symptoms usually subside drastically when Chloromycetin is used. In such cases widal reactions are not necessarily of clinical significance and it generally difficult to make a definite diagnosis when antibiotics were given in the early stage of diseases. Bacteriological and immunological studies were made in October ?? ?? typhoid fever which occurred in a limited epidemic form among the students of Nursing School of Kyungpook University. Routine culture study, biochemical and immunological reactions were performed to identify the isolated micro-organism of the disease as well as to detect the development of widal antibody in the patients who took various doses of Chloromycetin indiscriminately ranging total amount of 1.5 to 25 gr. in the entire course of the disease. In order to observe the antibody formation in the experimental animals after the use of Chloromycetin 6 groups of rabbits were inoculated with bacterial suspension and this was followed by oral adminstration of chloromycetion 100㎎ /㎏. body wt. daily. The duration of Chloromycetin administration varied in each group. Among the entire 14 patients the micro-organism was isolated in 7 cases, and all strains were indentified by means of biochemical and immunological procedures. These strains were slightly resistant to various antibiotics as compared to the original strains of Typhoid A-58. Widal antibodies of the patients were generally lower than the previously accepted level of antibody and correlate with the experimental results. However, antibody titer tested by killed bacterial suspension prepared with Chloromycetin has not been different substantially from that which was prepared with heat.

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