花粉生長에 미치는 石灰이온의 作用과 그 濃度, 酸性度 및 溫度의 相互關係에 對하여

郭炳華 한국식물학회 1965 Journal of Plant Biology Vol.8 No.1-2

花紛生長을 促進하는 Ca의 各種 濃度와 酸性度 그리고 溫度의 相互關係를 便利한 供試植物인 Crinum asiaticum와 Cryptostegia grandiflora의 花粉을 利用하여 人工培養하여 硏究한 結果 다음 事項을 알 수 있었다. 試驗結果는 Ca의 效果를 더욱 雇著히 나타내는 花紛管伸長度로써 主로 檢討하였다. Ca의 濃度가 높으면 花粉生長이 더욱 促進되는데 Ca濃度가 높을수록 最適 pH는 높아져 弱酸性에서 弱聾基性側으로 옮겨 갔다. 低溫에 있어서는 Ca效果가 全然 나타나지 않고 pH變化에 依한 花粉管伸長度에는 別差가 없었으나 高溫에서 비로소 Ca添加의 效果가 나타났을 뿐만 아니라 弱騷基性 培養液이 가장 좋았으며 그때 Ca를 添可하면 一層 그 效果를 增加시켰다. 높은 pH에서 자라나는 花粉은 低溫보디 高溫에서 特히 Ca의 效果가 顯著히 나타났는네 이것은 花粉管壁에 있어서 "Metabolis"한 過程을 밟아 合成된 Pectin 質에 "Non-metabolic"한 Ca의 吸着現象에 衣하여 主로 花紛管의 强靭度가 增加된 結果 花紛生長의 促進作用이 根因된 것이라 論議하였다. Interaction occuring among the differebt Ca concentrations, pH and te temperalures in the promotive effect of Ca in pollen growth was studied by using pollen from Crinum asiaticum and Cryptostegia grandiflora. Data for pollen tube elongation were found to be more indicative of representing the promotive action of Ca ion in pollen growth than those for pollen germination, and were served to evaluate the experimental results. The pollen growth increased as the concentration of Ca increase. The optimal pH range for pollen growth shifted from the lower pH to the higher as the concentration of Ca increase. The characteristic Ca effect was disappeared, and no pH effect at various ranges was observed when pollen grains were grown at the low temperature (8℃). The Ca effect became quite pronounced if temperature were raised. The Ca effect became even more striking if the condition was in higher pH ranges (weak alkaline). Higher pH ranges were found to be more favorable for the Ca action, whereas higher temperature was required to bring about more pronounced Ca effect. Thus, the longest pollen tube was obtained with the highest pH, temperature adopted for the medium supplemented with Ca in the present experiment, and the shortest tube with the lowest temperature applied at the highest pH. Pectin synthesis in pollen tube was considered as a metabolic process, whereas Ca binding in pectin of the pollen tube wall as non-metabolic in nature. Disappearance of Ca effect at the low temperature was probably brought about by blocking the metabolic synthesis of pectin, and nonmetabolic Ca binding seems to take place more extensively with higher concentrations of Ca and at higher pH levels than the lower.

에틸렌 생합성 억제제가 양란 Cymbidium의 절화 수명에 미치는 영향

郭炳華(Kwack Beyoung Hwa),徐廷南(Suh Jung Nam),金翰均(Kim Han Kyun) 한국원예학회 1996 Horticulture, Environment, and Biotechnology Vol.37 No.1

葯帽除去와 除雄이 절화 심비디움의 노화에 미치는 영향과 AVG의 역전효과

郭炳華(Kwack Beyoung Hwa),徐廷南(Suh Jung Nam),金翰均(Kim Han Kyun) 한국원예학회 1996 Horticulture, Environment, and Biotechnology Vol.37 No.2

Ethrel 의 농도 , 처리시기 및 질소농도별 시비의 (施肥) 잎담배 성숙에 미치는 영향

곽병화 (Beyoung Hwa Kwack) 한국식물학회 1974 Journal of Plant Biology Vol.17 No.1

The most commonly grown economical and flue-cured tobacco cultivar Yellow Special A was used in pot-culture tests in order to study Ethrel(2-chloroethyl phosponic acid) effects on accelerating maturity of tobacco leaves in relation to the most adequate level of the chemical useful for field growing, nitrogen level in soil for the most pronounced response, and the most suitable spray period during the growth stages of pre-, post- and topping periods. The following conclusions, thus, were obtained from the present studies; 1. 500ppm Ethrel spray was reconfirmed to be adequate in the practical applications, although the extent of yellow-ripening of tobacco leaves was increased as the Ethrel level increased. The highest level treated resulted in causing chemically damaged lesions on leaves and early defoliation. 2. Ethrel-treated leaves showed deeper yellowish tinge to them than those without treatment, while different levels of the chemical had less influence on the tinge. 3. An adequate level of nitrogen supply to plants favored the Ethrel response, whereas either very low or high level of nitrogen in the soil lowered the chemical effect on accelerating the yellow-ripening. When carbohydrates versus total nitrogen ratio became relatively high, the condttion brought out some outstanding Ethrel effects. 4. Chlorophyll level of leaves increased as soil applications of nitrogen level increased, and that also increased carotenoid level of the tobacco leaves. Ethrel-treated leaves showed deeper orange tinge than those without treatment, while the highest level of nitrogen application showed the deepest orange tinge to tobacco leaves. 5. Pre-topping treatment(12 days before topping and flowering) resulted in almost no Ethrel response, and that treatment right on the day of topping, showed response of yellow-leaf ripening at nearly bottom-half leaves of a tobacco plant. The post-topping treatment(12 days after topping) made plants showing full response of Ethrel from bottom to the top leaves of tobacco plant in accelerating the leaf maturity. 6. The extent of Ethrel responses on accelerating yellow-ripening of tobacco leaves was discussed for the modifying influenees brought about by certain environmental factors. Discussions were also made about the possible practical applications(particularly for pre-rice planting) and quality difference that may be caused by such growth environments.

수도 (水稻) IR 667 의 적고현상에 미치는 질소의 영향에 관한 연구

곽병화(Beyoung Hwa Kwack),구영서(Yung Suh Koo) 한국식물학회 1971 Journal of Plant Biology Vol.14 No.4

Pot and paddy field tests were conducted to study red discoloration of rice var. IR 667 leaves with reference to the leading Korean native variety Jinhung and Paldal, with the following results: 1. Minor elements such as Mn, Fe, B, Al, Ca and Si had no influence on the discoloration, but a supply of various soluble nitrogen compounds completely restricted it. The more prosperous the growth of IR 667 with nitrogen, the more severe the discoloration appears when nitrogen absorption becomes limited. 2. Chlorotic pigments extracted from both IR 667 and Jinhung were compared spectrophotometrically, and found to have different spectral peaks. IR 667 had peak closer to red than Jinhung, indicating the characteristic of the variety. IR 667 was observed to be more sensitive to nitrogen deficiency than Jinhung or the other japonica variety. 3. It was concluded that all the factors limiting nitrogen supply for IR 667 growth, such as low nitrogen application, restriction of root respiration (low temperature, poor drainage, toxic gases or substances in the root zone, etc.) and pest injuries, would result in the appearance of the so-called red discoloration, because of the reduction in nitrogen uptake. Since, the discoloration of IR 667 is varietal characteristic when grown in Korea, control of it may be beneficial cultural practice in increasing grain yield, although the increased succeptibility to pests and a drop in the rate of maturity due to relatively high nitrogen level in the leaves may result in an unexpected drop in yield. It is anticipated that further exploration conducted from practical point of view will establish the relationships between the extent of red discoloration, nitrogen availability and grain yield in IR 667.

화분관 (花紛管) In Vitro 생장의 굴수성에 (屈水성) 대하여

곽병화 (Beyoung Hwa Kwack) 한국식물학회 1971 Journal of Plant Biology Vol.14 No.2

Zephyranthes candida, Narcissus pseudonarcissus and Crinum asiaticum pollen were placed near their pistil parts respectively on agar cultural media (microslides) containing 10% sucrose and 100㎎/1 boric acid plus 1% agar with or without calcium and some other calcium-supporting inorganic salts. If fresh pistils (100% moisture) were used pollen grew toward their pistil parts, showing positive tropism. This was also true when combinations among three different species were made. Pollen tubes grew away from the pistils if they were dried (below 10% moisture), showing negative tropism. Pollen could not show sny tropic growth and thus grew at random of all directions if the pistil parts were incompletely dried (approximately 50% moisture). The similar tropic responses of pollen-tube growth with the three species could be demonstrated with either wet or dried tooth-pick segments. Calcium ions in the basic medium merely promated pollen-tube growth and so eitherpositive or negative tropism became rather distinctive, but they were not tropically active. Pollen tubes grow toward pistil parts with more moisture content and seem to be hydrotropically sensitive. This was assumed due to the cohesive force existing in water molecules.

콩과식물화분의 Leucine Aminopeptidase 검출과 그 Isozyme 에 대하여

곽병화 (Beyoung Hwa Kwack) 한국식물학회 1970 Journal of Plant Biology Vol.13 No.2

Identification and observations of leucine aminopeptidase (LAP) and multiple molecular forms of the enzyme, isozymes, were made with a technique of starch-gel eletrophoresis for various legume pollen. Plants tested other tnan Leguminosae demonstrated either no indication of the presence or at least trace of the enzyme and the isozymes, although all legume pollen tested showed strong LAP patterns. The electrophoretic patterns of LAP failed to be shown if the extracts were heated or otherwise denatured. Extent of zymogrammatic appearance of LAP and the isozymes were characteristic of a species.

In Sem - Vitro 달맞이꽃화분 (花紛) 의 석회에 의한 생장촉진과 DNP 및 저온의 그 억제작용에 관하여

곽병화(Beyoung Hwa Kwack),윤경은(Kyung Eun Yoon) 한국식물학회 1969 Journal of Plant Biology Vol.12 No.2

The promotive effect of Ca in semi-vitro culture of Oenothera biennis pollen was by and large identical to that of in vitro systems. This promotive effect became additive when boron was supplemented to the media and even more so if K was further supplemented. No such Ca action waa observed when performed under the conditions of relatively low temperature. An uncoupling agent of ATP in respiration, DNP, inhibited the promotive action of B, but not that of Ca. The inhibitory effect of DNP was greater at low temperatures. IAA was rather inhibitory on pollen growth in semi-vitro culture, even much greater than it if DNP was supplemented to the IAA-containing media.

문주란 花粉의 in situ 生長極性에 關하여

郭炳華(Kwack Beyoung hwa) 한국원예학회 1970 Horticulture, Environment, and Biotechnology Vol.8 No.-

적외선형광장치현미경으로 본 화분관생장과 석회 및 aureomycin 의 상호작용에 대하여

곽병화 (Beyoung Hwa Kwack) 한국식물학회 1967 Journal of Plant Biology Vol.10 No.1-2

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