Vitamin E가 마우스 各臟器에 미치는 影響에 關한 病理組織學的 硏究 : 特히 조직비반세포에 關하여 Especially on the Tissue Mast Cells.

洪淑熹 고려대학교 의과대학 1966 고려대 의대 잡지 Vol.3 No.2

The study was undertaken to observe the influence of vitamin E (V.E) on the numerical changes of the tissue mast cells (T.M.C.) in the various tissues of mice. The mice were classified into non-treated group, vitamin E execessive group and vitamin E deficient group. The findings of the tissue mast cells in lung, liver, kidney, skin, skeletal muscle, esophagus, stomach, ileum, appendix, colon, testicle and ovary were studied and the following results were obtained. 1. The number of the T.M.C. in the aged mice (fed with normal diet for three months) was more decreased than that of the matured control one, especially in the skin and appendix. 2. The decreasing ratio of the T.M.C. was higher in the mice treated by the first three times of vitamin E injection than in the others. 3. In the vitamin E deficient group, amyloid degeneration of liver was observed. 4. In the vitamin E deficient group, the number of the T.M.C. in the esophagus, stomach, ileum and colon was more decreased than that of the aged control group. 5. In the vitamin E supplement group, after being fed with V.E deficient diet for three months, the number of the T.M.C. in the skin and esophagus was decreased than that of the vitamin E deficient group. 6. In the vitamin E excessive group (V.E excessively injected into the aged one), the number of the T.M.C. was decreased than that of the aged mice in the skin, esophagus, stomach, ileum and, colon and was decreased in the skin and esophagus than that of the V.E deficient animal. 7. The variations of the amount of vitamin E in the body made tissue mast cell counts decrease in various tissues.

How to start niraparib in real-world Asian ovarian cancer patients?

홍숙희 대한부인종양학회 2021 Journal of Gynecologic Oncology Vol.32 No.2

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용혈이 각종 생화학 검사결과에 미치는 영향

홍숙희,김용호 인제대학교 1981 仁濟醫學 Vol.2 No.3

화학검사의 정확성과 결과 해석에 영향을 미치는 요인은 에러가지가 있지만 그중 응혈은 여러가지 이유에서 중요한 의의를 가진다. 저자들은 현재 부산 백병원 임상병리과에서 일상 행해지고 있는 각종 화학검사법에 대해 용혈이 미치는 영향을 관찰하기위해 혈청내 유리 혈색소를 점차 증가시키면서 검사 결과치와의 상호관계를 정량적으로 관찰하였든 바, 대부분의 화학검사가 용혈의 심한 정도에 비례하여 영향을 받고 있고 그중에서도 효소종목이 가장 큰 변화를 보여 LDH, acid phosphatase, SGOT는 정상치를 벗어나 몇 배의 증가를 보이고 있다. 아주 소량의 용혈이 있어도 각종 검사는 비용혈 혈청에 비해 통계적으로도 유의한 변화를 보여 용혈에 의한 영향은 명백하므로 용혈이 있는 검체는 그 결과해석에 신중을 기해야 한다. Hemolysis is a common cause of unsatisfactory serum samples obtained for clinical chemistry analysis, because it affects the accuracy of the chemical determination and the interpretation of the laboratory results. We have studied the hemolytic effects on various chemistry values quantitavely, determined in Busan Paik Hospital Laboratory everday, by serially increasing serum flee hemoglobin concentration on nonhemolyzed control pooled serum. The results obtained are as fellows: 1.Most of the chemistry values are affected significantly by hemolysis, in direct proportion to the degree of hemolysis. 2.Among the chemical determinations, enzyme groups are most affected by hemolysis. Electolytes except for potassium are least affected and nonenzyme groups are noticeably affected by hemolysis. 3.Of the enzymes, LDH appears to be most sensitive to hemolysis and the total increase of 1485.4U/ml results in 4.6 folds higher than nonhemolyzed control serum. GOT, acid phosphatase, alkaline phosphatase, GPT, and Gamma-GTP are order of increasing rate by hemolysis. 4.Of the electolytes, potassium is most sensitive to hemolysis and shows an increase of 69.7% compared with nonhemolyzed control serum. Lesser effects are observed in inorganic phosphate, chloride and calcium. 5.Of the non-enzyme groups, BUN is markedly increased by hemolysis. Total protein and triglyceride are also significantly increased but glucose is rather decreased. 6.Several chemistry values, enzymes in particular, potassium, and non-enzymes, show significant changes even though in trace hemolysis, and we should dive careful attention in the interpretation of the results obtained from hemolyzed serum.

자기조절학습전략 훈련이 독해학습부진아의 독해력과 학업성취도에 미치는 영향

홍숙희,박남수 대구대학교 사범대학 부설 교육연구소 2009 敎育硏究 Vol.4 No.2

혈당 조절 지표로서 HbA1c와 Fructosamine Assay의 유용성에 관한 비교 연구

홍숙희,함건주 인제대학교 1989 仁濟醫學 Vol.10 No.2

당뇨병 환자의 혈당 조절 평가에 이용되는 검사 종목을 비교한 바 혈중 당화 단백의 일종인 fructosamine 측정이 HbAlc 측정보다 검사 방법이 비교적 간편하고 신빙성이 있어 유용한 검사로 사료된다. The non-enzymatic glycosylation of proteins, especially hemoglobin as the most abundant blood protein, and the relationship of such glycosylation to diabetes mellitus have been the subject of much investigation in the monitoring of diabetics. It is known that other proteins of blood and tissue can be glycated in the hyperglycemic status to the same extent as haemoglobin. Ordinarily, methods used in the clinical laboratory for the measurement of glycated proteins up to now have concentrated on the extimation of HbAlc, although the method is technically demanding, time consuming and high cost. The fructosamine assay in serum has recently been described by Baker and coworkers as a new parameter for the estimation of protein glycation. The results of the fructosamine assay which was compared to HbAlc assay are as follows: 1.There was a significant correlations between fructosamine assay and HbAlc assay (r=0.70. P< 0.001). 2.Fasting blood glucose levels and fructosamine concentrations were clearly correlated (r=0.71. P< 0.001). We find that the fructosamine assay provides simple reliable assessment of glucose control in diabetic patients and more usefulness in short term mornitoring than HbAlc assay.

간세포암과 간내담관암 조직에서 GLUT-1 당 수송 단백의 발현 양상

홍숙희 한국간담췌외과학회 2000 한국간담췌외과학회지 Vol.4 No.1

Background/Aims : Malignant cells exhibit increased glycolytic metabolism, and in many cases increased glucose transporter gene expression. We studied the expression of the glucose transporters in hepatocellular carcinoma(HCC) and intrahepatic cholangiocarcinoma(CC). also, examined the usefulness of Glut-1 glucose transporter in the discrimination of HCC from CC. Methods and Results : 23 HCC, 15 CC and 8 normal liver tissues were investigated immunohistochemically with Glut-1 glucose transporter. Immunostaning was regarded as positive when more than 5% of cells were stained. Among 38 liver tumor cases, Glut-1 was stained in 15(40%). in 2(9%) of 23 HCC and in 13(87%) of 15 CC were positive.(P=0.001) In HCC, underlying cirrhosis, 18(78%) were negative.(P=0.04) Other prognostic factors: histologic type, lymph node metastasis, distant metastasis did not show any significant correlation. Conclusion : Differential diagnosis between HCC and CC could be made by Glut-1 glucose transporter expression Background/Aims : Malignant cells exhibit increased glycolytic metabolism, and in many cases increased glucose transporter gene expression. We studied the expression of the glucose transporters in hepatocellular carcinoma(HCC) and intrahepatic cholangiocarcinoma(CC). also, examined the usefulness of Glut-1 glucose transporter in the discrimination of HCC from CC. Methods and Results : 23 HCC, 15 CC and 8 normal liver tissues were investigated immunohistochemically with Glut-1 glucose transporter. Immunostaning was regarded as positive when more than 5% of cells were stained. Among 38 liver tumor cases, Glut-1 was stained in 15(40%). in 2(9%) of 23 HCC and in 13(87%) of 15 CC were positive.(P=0.001) In HCC, underlying cirrhosis, 18(78%) were negative.(P=0.04) Other prognostic factors: histologic type, lymph node metastasis, distant metastasis did not show any significant correlation. Conclusion : Differential diagnosis between HCC and CC could be made by Glut-1 glucose transporter expression

Vitamin E가 마우스 各臟器에 미치는 影響에 關한 病理組織學的 硏究

洪淑熹 首都醫科大學 1966 수도 의대 잡지 Vol.3 No.2

GAS CHROMATOGRAPHY를 利用한 STAPHYLOCOCCUS 菌屬의 脂肪酸 分布에 관한 硏究

金基東,金春元,咸健珠,洪淑姬 한양대학교 의과대학 1988 한양의대 학술지 Vol.8 No.1

Whole-cell fatty acid methylester profiles of the Genus Staphylococcus were determind by gas chromatogrphy with application of the flexible, fused-silica capillary column for increased resolution. The results of the study were as follows: 1. The major cellular fatty acids were C15:0 Iso, C15:0 Anteiso, C17:0 Iso, C17:0 Anteiso, C18:0, C20:0. 2. Staphylococcus aureus-GC subgroup A contained relatively large amount of C15:0 Anteiso and C17:0 Anteiso than subgroup B. 3. Staphylococcus aureus-GC subgroup B contained relatively large amount of C20:0 than subgroup A. 4. The cellular fatty acid of the Genus Staphylococcus were similar,but characterized by relatively large amounts and small amounts as follows: 1) Staphylococcus cohnii contained large amounts of C15:0 Iso, C15:0 Anteiso, C17:0 Iso, C17:0 Anteiso and contained small amounts of C20:0 than Staphylococcus aureus. 2) Staphylococcus haemolyticus contained large amounts of C15:0 Anteiso, C17:0 Iso, C17:0 Anteiso and contained small amounts of C20:0 than Staphylococcus aureus. 3) Staphylococcus simulans contained large amounts of C15:0 Iso, C15:0 Anteiso, C17:0 Iso, C17:0 Anteiso an contained small amounts of C20:0 than Staphylococcus aureus. 4) Staphylococcus epidermidis contained large amounts of C15:0 Anteiso than Staphylococcus sureus. 5) Staphylococcus warneri contained large amounts of C15:0 Anteiso than Staphylococcus aureus. 6) Staphylococcus caprae contained large amounts of C15:0 Anteiso and contained small amounts of C20:0 than Staphylococcus aureus. In conclusion, this study provided useful information for rapid detection and identification of several acterial groups or species.

Changes in expression of cell cycle regulators after G1 progression upon repetitive thioacetamide treatment in rat liver

홍숙희,이기등,정준영,조경숙,박석희,김인후,정진숙 생화학분자생물학회 2002 Experimental and molecular medicine Vol.34 No.5

Phospholipase Cγ1 (PLCγ1) plays an important role in controlling cellular proliferation and differ-entiation. PLCγ1 is overexpressed in some tu-mors, and its overexpresion induces solid tumors nisms underlying PLCγ1-induced cell proliferation are not fuly understood. Here we show that over-expression of PLCγ1 highly phosphorylated glyco-gen synthase kinase-3β (GSK-3β) at serine-9 in 3Y1 fibroblasts. Inhibition of protein kinase C (PKC)s with GF109203X abrogated GSK-3β phosphoryla-tion by PLCγ1. We also found that steady-state level of cyclin D1 protein, but not cyclin D1 mRNA, was highly elevated in response to serum stimu-lation in PLCγvector-transfected cels. Since GSK-3β is involved in cyclin D1 proteolysis in response to mitogenic stimulation, PLCγ1-mediated GSK-3β phosphoryla-tion may function as a regulation of cyclin D1 accumulation in PLCγ1-overexpressing cels.

한국인 일반 여성의 HPV 감염 유병율 -부산지역 일반 여성에서의 HPV DNA 및 항 VLPs 항체 양성 빈도 -

홍숙희,이덕희,신해림,Hong, Sook-Hee,Lee, Duk-Hee,Shin, Hai-Rim 대한세포병리학회 2004 대한세포병리학회지 Vol.15 No.1

To investigate a population-based survey of the prevalence of human papillomavirus (HPV) infection in South Korea, we performed Papanicolaou smears and tests for HPV DNA and anti-HPV antibody detection in 909 sexually active general women (age range; 20-74 years, median 44 years) who were randomly selected residents from S district of Busan City. The presence of DNA of 36 different HPV types was detected by means of a GP 5+/6+ primer-mediated PCR enzyme immunoassay in cervical exfoliated cells, and IgG antibodies against L1 virus-like particles (anti-VLPs) of 5 HPV types 16, 18, 31, 33, and 58 were tested by means of enzyme linked immunoassay. The incidence of cytologic abnormality was 5.2% in Pap smear. The positive rate of HPV DNA was 10.4%, high in young women younger than 35 years old and proportionally increased according to the cytologic grades. The most often found HPV type was HPV 70, followed by HPV 16 and 33, and high-risk HPV types were more frequent in women younger than 35 years old. The most common HPV type in abnormal cytologic smears was HPV 16, followed by HPV 58 and 66. Anti-VLPs was positive in 19.7% and the frequent anti-VLPs type was against HPV 18, followed by HPV 31 and 16. The concordance between the markers for each specific HPV type was noted in 10 women and HPV 16 was the most frequent one. The incidence of multiple HPV infection was 18.9% and that of multiple anti-VLPs antibodies was 31%. Among 103 self-reported virgins, 4.9% had anti-VLP antibodies.