호밀(Secale cereale L .)Esterase 동위효소의 유전적 연구

방재욱,최혜운 한국유전학회 1990 Genes & Genomics Vol.12 No.2

Esterase isozymes (EST) in endosperm, root and shoot of rye seedlings were determined using polyacrylamide gel electrophoresis and the effect of B-chromosomes (Bs) on EST zymogram phenotypes was studied. The number and staining intensity of EST bands vary depending on the tissues applied. EST-A is present in all the tissues of the rye seedlings, whereas EST-B is revealed as the coleoptile-specific esterase. EST-C exhibits a two-banded phenotype. EST-D showed different band patterns among tissues. In leaf EST patterns of rye with Bs and without Bs, EST-D showed difference in pattern wtith the presence of Bs. Individuals without Bs have two specific EST bands, whereas plants with 2Bs and 4Bs have three and four specific EST bands respectively. The results reported in this paper lend evidence for the genetic effect of Bs on EST patterns in rye.

배추 유전체연구의 현황

임용표,김정희,방재욱 中央大學校 食糧資源硏究所 1996 食糧資源硏究所 論文集 Vol.8 No.1

ABSTRACTThe commercial value of Chinese cabbage and its usage in Kimchi are well known in Korea. The need to understand the genomic structure and its organization is required more than ever not only to be prepared to UR (making better variety) but to satisfy the basic human questions such as “How genes are organized in the genome”. Thus, in my lab, construction of the genomic library of Chinese cabbage is being performed. We are using pBAC which was originally made by Dr. M. Simon at Caltech, as a cloning vehicle. This vector is known to harbor up to 300 Kb size DNA insert and very stable in E. coli. Since the genomic size of chinese cabbage is 750Mb, only 20,000 clones will cover 5x of the entire genome. With the current method it will be able to finish the 5x library in one year.The contig of library will be generated using 3 different methods. First we are going to use restriction finger printing with a powerful computer program. Second, the AFLP and RAPD will be employed to sort each chromosomes. The AFLP and RAPD map will be generated very fast because we already have over 100 established recombinant inbred lines. Now, we already developed over 320 AFLP and RAPD markers, and constructed genetic map. These makers will be used as the sources for physical mapping. Third, over 5,000 RFLP probes which was generated from EST will be also employed to aid the construction of Chinese cabbage genetic map. Recently, chromosome analysis technologies were rapidly developed, such as FISH, GISH, and chromosome microdissection methods. These methods also will apply to genome analysis of Chinese cabbage. All of these methods and our progress so far will be presented briefly.

한국산 무릇(Scilla scilloides complex)의 세포유전학적 연구 : Ⅱ. 제주도 집단에서 게놈의 분포 Ⅱ. Distribution of Genomes in Chejudo Populations

方在旭,崔慧雲 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

Cytogenetic analyses were done in thirteen natural populations of Scilla scilloides complex collected from Chejudo. Six genotypes, BB (2n=18), ABB (2n=26), AABB (2n=34), ABBB (2n=35), AABBB (2n=43) and AAABBB (2n=51) were observed, where A and B denote genomes with χ=8 and χ=9, respectively. Genotypes BB, ABB and AAABBB were found for the first time in Chejudo populations. Genotypes BB and ABB were never reported from the Korean populations. Six of the populations investigated were mixed populations of two or three cytogenetic types, and others were pure populations consisting of BB (1), AABB (4) and AABBB (2). AABB genotypes were predominant over other genotypes and AABBB was the next. AA genotypes growing in the mainland of Korea was not found. It was suggested that the Chejudo populations of S. scilloides complex were closely related to the Japanese populations than mainland populations of Korea. The number of B-chromosome ranged from one to four. The B-chromosomes were all isochromosomes (F) and no chromosome fragment (f) was found. The frequencies of B-chromosome were 75% in BB and AABBB genotypes, respectively. The frequency of B-chromosome in AABB plants was similar to that of AABB plants reported in the mainland populations.

한국 무릇(Scilla scilloides Complex)의 세포유전학적 연구 : Ⅲ. BB 게놈의 핵형과 B염색체 조성 Ⅲ. Karyotype of Cytotype BB and B-Chromosome Composition

方在旭,崔惠雲 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

A karyotype of cytotype BB plant in Scilla Complex was established and the frequency of B-chromosomes were investigated. Chromosome complements of BB genome were composed of five pairs of subtelocentric and four pairs of metacentric chromosomes. Chromosome 1 has satellite with nucleolar organizer. Polymorphism was found in chromosome 2. The karyotype of cytotype BB will be available for analysis of genome composition in various cytotypes of S. scilloides Complex. The frequency of B-chromosome was 78.6%. Numbers of B-chromosome ranged from 1 to 4 and plants with 2B-chromosomes were predominant (57.2%). Two types of B-chromosomes, F and F', were found; F is a large iso-chromosome and F' a small one.

호밀 (Secale cereale L.)B染色體의 Transmission에 관한 硏究

方在旭 木浦大學校基礎科學硏究所 1984 基礎科學硏究誌 Vol.2 No.-

The transmission of B chromosomes in PALDANG rve(Secale cereale L.) was investigated. In the progeny of the cross in population of Bs plants average number of B chromosome per plant increased 2 to 2.5 in first generation. In the progeny of 2Bs×2Bs, the B chromosome number per plant was found to be 2.1 and in the case of the crosses between 4Bs( ♀) and 2Bs (♂), it appeared 3.5, 3.1 and 3.2 respectively.

Falsely increased bispectral index score during deep hypothermic circulatory arrest in cardiac surgery

방재욱,손효정,이은호,함경돈,최인철 대한마취통증의학회 2012 Korean Journal of Anesthesiology Vol.63 No.4

대학 총장론

방재욱,Bang, Jae-Uk 한국대학교육협의회 1994 大學敎育 Vol.71 No.-

The effect of positive-end expiratory pressure on oxygenation during high frequency jet ventilation and conventional mechanical ventilation in the rabbit model of acute lung injury

방재욱,하승일,최인철 대한마취통증의학회 2012 Korean Journal of Anesthesiology Vol.63 No.4

Background: The use of positive end expiratory pressure (PEEP) in patients with acute lung injury (ALI) improves arterial oxygenation by alleviating pulmonary shunting, helping the respiratory muscles to decrease the work of breathing, decreasing the rate of infiltrated and atelectatic tissues, and increasing functional residual capacity. In a rabbit model of saline lavage-induced ALI, we examined the effects of PEEP on gas exchange, hemodynamics, and oxygenation during high frequency jet ventilation (HFJV), and then compared these parameters with those during conventional mechanical ventilation (CMV). Methods: Twelve rabbits underwent repeated saline lavage to create ALI. The animals were divided in 2 groups: 1) Group CMV (n = 6), and 2) Group HFJV (n = 6). In both groups, we applied 2 levels of PEEP (5 cmH2O and 10 cmH2O) and then measured the arterial blood gas, mixed venous blood gas, and hemodynamic parameters. Results: With administration of PEEP of either 5 cmH2O or 10 cmH2O, the arterial oxygen content of both groups was increased, although without statistically significant differences between groups. On the contrary, the arterial carbon dioxide content was significantly decreased in the HFJV group, as compared with the CMV group, during the entire experiment. Furthermore, there was significant decreases in mean arterial pressures in both groups with a PEEP of 10 cmH2O. Conclusions: The application of PEEP in rabbits with ALI effectively improves oxygenation in either HFJV or CMV. Background: The use of positive end expiratory pressure (PEEP) in patients with acute lung injury (ALI) improves arterial oxygenation by alleviating pulmonary shunting, helping the respiratory muscles to decrease the work of breathing, decreasing the rate of infiltrated and atelectatic tissues, and increasing functional residual capacity. In a rabbit model of saline lavage-induced ALI, we examined the effects of PEEP on gas exchange, hemodynamics, and oxygenation during high frequency jet ventilation (HFJV), and then compared these parameters with those during conventional mechanical ventilation (CMV). Methods: Twelve rabbits underwent repeated saline lavage to create ALI. The animals were divided in 2 groups: 1) Group CMV (n = 6), and 2) Group HFJV (n = 6). In both groups, we applied 2 levels of PEEP (5 cmH2O and 10 cmH2O) and then measured the arterial blood gas, mixed venous blood gas, and hemodynamic parameters. Results: With administration of PEEP of either 5 cmH2O or 10 cmH2O, the arterial oxygen content of both groups was increased, although without statistically significant differences between groups. On the contrary, the arterial carbon dioxide content was significantly decreased in the HFJV group, as compared with the CMV group, during the entire experiment. Furthermore, there was significant decreases in mean arterial pressures in both groups with a PEEP of 10 cmH2O. Conclusions: The application of PEEP in rabbits with ALI effectively improves oxygenation in either HFJV or CMV.

생명 에세이(4) - 유전자 발현에 담긴 삶의 이야기

방재욱 본질과 현상사 2019 본질과 현상 Vol.57 No.-

호밀 (Secale cereale L.) Esterase 동위효소의 유전적 연구

방재욱,최혜운 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-

Esterase isozymes (EST) in endosperm, root and shoot of rye seedlings were determined using polyacrylamide gel electrophoresis and the effect of B-chromosomes (Bs) on EST zymogram phenotypes was studied. The number and staining intensity of EST bands vary depending on the tissues applied. EST-A is present in all the tissues of the rye seedlings, where as EST-B is revealed as the coleoptile-specific esterase. EST-C exhibits a two-banded phenotype. EST-D showed different band patterns among tissues. In leaf EST patterns of rye with Bs and without Bs, EST-D showed difference in pattern wtith the presence of Bs. Individuals without Bs have two specific EST bands, whereas plants with 2Bs and 4Bs have three and four specific EST bands respectively. The results reported in this paper lend evidence for the genetic effect of Bs on EST patterns in rye.