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A-42 : Micropatterning for Olfactory Bulb and Hippocampus Neuronal Network Structures
안동준,최혜선 한국화학공학회 2007 화학공학의이론과응용 Vol.10 No.2
The control of neuronal cell adhesion and growth in artificially defined networks in vitro was developed for the study of neuronal signals propagation. Appropriate analysis is still greatly hindered by inherent morphological complexity of cultured neural network without any control in location of and/or synaptic connection between neurons. In order to build neural networks on a substrate, it is required to modify the substrate surface suitable for patterning neurons. Soft contact printing with a polydimethyl-siloxane (PDMS) stamp, which was fabricated by photolithography and molding, was used for controlling selective neuronal cell adhesion and growth. The experimental results indicate that the PDL(cytopilic) pattern stamping on PEG (cytophobic) background is effective in growing the hippocampal neuron cells and olfactory cells selectively along the PDL pattern. To optimize neuron growth and pattern forming neural networks, we counted cell body on node and neurits extended along track. And we traced how the neurons found PDL(cytophilic) tracks using dendrites to locate themselves on tracks.
안동준,윤태원,김한웅 안성산업대학교 1994 論文集 Vol.26 No.-
In this paper, the fuzzy LMS algorithm where the convergence coefficient is computed by a fuzzy logic controller was proposed. The proposed fuzzy LMS algorithm showed better convergence property and stability than conventional LMS algorithms. The estimation error and misadaptation degree were used for input of the fuzzy logic controller. In a airconditioning duct case, various conditions were investigated to design active noise controllers. A case with acoustic feedback, the proposed algorithm showed good performances through computer simulations.
Pseudorabies Virus의 Major Capsid Protein 유전자의 클론닝과 Baculovirus Vector System에 의한 발현
안동준,전무형,송재영,박종현,현방훈,장경수,안수환 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-
Pseudorabies is caused by Pseudorabies virus (PRV: Aujeszky's disease virus) of Herpesviridae that is characterized by 100 to 150nm in size with a linear double-stranded DNA molecule with of approximately 90 × 10 exp (6)Da. This disease affects most of domestic animals such as swine, cattle, dog, sheep, cat, chicken, etc. causing high mortality and economic losses. In swine, young piglets show high mortality and pregnant sows, reproductive failures. However the adult swine reveals no clinical signs in general. But they become a carrier state and play an importnat role for propagation of the disease. In this study, the nucleotide sequence of major casid protein gene of PRV, Yangsan strain isolated from the diseased swine in Korea was analyzed, and the recombinant MCP was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. As result, in BamHI digestion, MCP gene locus of PRV YS strain showed different from that of Indiana S strain. The patterns of enzyme mapping were also found to be unidentical each other. The sequence of the MCP gene partially analyzed showed 98.09% identity to Indiana S strain. The expression of MCP in Sf-9 cell cotransfected by pVLMCP-44 baculovirus expression vector was characterized by Southern blot hybridization, immunofluoresent and immunocytochemical tests, SDS-PAGE and Western blotting. The rMCP with M.W. 142kDa was most effectively expressed in Sf-9 cells at the 3-4th days post inoculation of the recombinant baculovirus by 2 moi.