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Karyotypes of Three Somaclonal Variants and Wild Plants of Allium tuberosum by Bicolor FISH
Do, Geum-Sook,Seo, Bong-Bo,Pak, Jae-Hong,Kim, In-Sun,Song, Seung-Dal 한국식물학회 2000 Journal of Plant Biology Vol.43 No.3
Using the fluorescence in situ hybridization (FISH) technique, we conducted karyotype analyses to identify the lost chromosomes in three somaclonal variants obtained from tissue culture of wild Allium tuberosum (2n=4X=32). The three lost chromosomes of the At29 variant (2n=29) were all chromosome 2, the two for At30 (2n=30) were chromosomes 7 and 8, and At31 was missing chromosome 2. Chromosome compositions of these variants were confirmed as being fixed lines during two years of greenhouse cultivation. The bicolor FISH technique, involving both 5S and 18S-5.8S-26S ribosomal RNA genes as probes, was used to assign chromosomal locations and to confirm whether the lost chromosomes contained any rRNA markers. The 5S rRNA gene signals in all variants as well as the wild type were detected as two sets, one on the intercalary region of the short arm of chromosome 3, the other on the intercalary region of the long arm of chromosome 6. One 18S-5.8S-26S rRNA gene site on the secondary constriction included a flanking satellite and terminal region on the short arm of chromosome 8. Signals of the 18S-5.8S-26S rRNA gene in At30 showed in only three chromosomes, indicating that one of the lost chromosomes was chromosome 8. Overall, three marker chromosomes were established by FISH, using rRNA multigene families.
Do, Geum-Sook,Seo, Bong-Bo The Korean Society for Integrative Biology 2000 Korean journal of biological sciences Vol.4 No.1
This study has demonstrated the molecular variation of 5S rRNA genes in 15 Allium subgenus Rhizirideum and 1 Allium subg. Allium. For cloning of the 5S rRNA genes, PCR products were obtained from amplification with oligonucleotide primers which were derived from the conserved coding region of 5S rRNA genes. These amplified PCR products were cloned and identified by FISH and sequence analysis. The 5S rRNA loci were primarily located on chromosomes 5 and/or 7 in diploid species and various chromosomes in alloploid species. The size of the coding region of 5S rRNA genes was 120 bp in all the species and the sequences were highly conserved within Allium species. The sizes of nontranscribed spacer (NTS) region were varied from 194 bp (A. dektiude-fustykisum, 2n=16) to 483 bp (A. sativum). Two kinds of NTS regions were observed in A. victorialis var. platyphyllum a diploid, A. wakegi an amphihaploid, A. sacculiferum, A. grayi, A. deltoide-fistulosum and A. wenescens all allotetraploids, while most diploid species showed only one NTS region. The species containing two components of NTS region were grouped with different diploid species in a phylogenetic tree analysis using the sequences of 5S rRNA genes and adjacent non-coding regions.
Jung, Youngae,Lee, Jueun,Kim, Ho Kyoung,Moon, Byeong Cheol,Ji, Yunui,Ryu, Do Hyun,Hwang, Geum-Sook The Royal Society of Chemistry 2012 The Analyst Vol.137 No.23
<P><I>Curcuma</I> is used to treat skin diseases and colic inflammatory disorders, and in insect repellants and antimicrobial and antidiabetic medications. Two <I>Curcuma</I> species (<I>C. aromatica</I> and <I>C. longa</I>) grown in Jeju-do and Jin-do were used in this study. Methanolic extracts were analyzed by <SUP>1</SUP>H NMR spectroscopy, and metabolite profiling coupled with multivariate analysis was applied to characterize the differences between species or origin. PCA analysis showed significantly greater differences between species than origins, and the metabolites responsible for the differences were identified. The concentrations of sugars (glucose, fructose, and sucrose) and essential oils (eucalyptol, curdione, and germacrone) were significantly different between the two species. However, the samples from Jeju-do and Jin-do were different mainly in their concentrations of organic acids (fumarate, succinate, acetate, and formate) and sugars. This study demonstrates that NMR-based metabolomics is an efficient method for fingerprinting and determining differences between <I>Curcuma</I> species or those grown in different regions.</P> <P>Graphic Abstract</P><P>This paper has described metabolite profiling to characterize the differences between <I>Curcuma</I> species grown in different regions by <SUP>1</SUP>H NMR spectroscopy coupled with multivariate analysis. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2an35397k'> </P>