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Wang, Weizhen,Zhang, Juan,Hu, Honghong,Yu, Baojun,He, Jintong,Yao, Tingting,Gu, Yaling,Cai, Zhengyun,Xin, Guosheng Asian Australasian Association of Animal Productio 2022 Animal Bioscience Vol.35 No.11
Objective: Inosine monophosphate (IMP) is a key factor that imparts of meat flavor. Differences in the IMP content in the muscles were evaluated to improve chicken meat quality. Methods: For this study, the IMP content was detected by high performance liquid chromatography. The gene expression profiles of Jingyuan chickens with different feeding patterns and different sexes were analyzed by RNA-sequencing (RNA-seq). Results: Breast muscle IMP content in free-range chickens was extremely significantly higher than that of caged chickens (p<0.01). Breast muscle IMP content in hens was also higher than that of cocks, but the difference was not significant. Correlation analysis showed that the breast muscle IMP content in caged hens and cocks was negatively correlated with the shear force, and the breast muscle IMP content in free-range hens was significantly negatively correlated with the shear force (p<0.05). The two key genes associated with IMP synthesis in chickens with different feeding patterns were glutamate-ammonia ligase (GLUL) and phosphodiesterase 10A (PDE10A). Bioinformatics analysis revealed that the GLUL and PDE10A genes are involved in glutamine biosynthesis and purine salvage pathways respectively. In addition, GLUL expression was positively correlated with the IMP content in caged and free-range chickens, and PDE10A expression was significantly positively correlated with the IMP content in caged and free-range chickens (p<0.05). Conclusion: These findings will facilitate the comprehension of the deposition of IMP in the muscles and thereby aid the process of selection and breeding of good quality local chickens.
( Milljae Shin ),( Doopyo Hong ),( Zhengyun Zhang ),( You Min Kim ),( Wookjong Lee ),( Jong Man Kim ),( Choon Hyuck David Kwon ),( Jae Won Joh ),( Sung Joo Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-
Purpose: Erythropoietin (Epo), through its specific erythropoietin receptor (Epo-R) may induce responses in a variety of non-hematopoietic tissues including malignant cells. In this study, we examined the expression of Epo-R in hepatocellular carcinoma (HCC) and tested the correlations of Epo-R expression with clinicopathological properties of HCC and with tumor recurrence. Methods: The study included 134 patients who underwent curative hepatectomy for HBV-related primary HCC. The medical records and laboratory or pathologic data from these patients were collected and analyzed. The expression of Epo-R mRNA and protein were evaluated by RT-PCR and western blot analysis, respectively. Results: We found intense expression of Epo-R mRNA in both non-tumor cirrhotic liver and HCC. Expression of Epo-R mRNA in the cirrhotic liver correlated significantly and negatively with tumor size, and positively with tumor cell differentiation and p53 staining; Epo-R mRNA expression in the HCC correlated with differentiation and p53 staining. The expression of Epo-R mRNA in cirrhotic liver or HCC was positively correlated with disease-free survival. Tumor recurrence showed stronger dependence on the expression of Epo-R protein in non-malignant cirrhotic liver than in the HCC. Conclusion: In HBV-related HCC, levels of Epo-R mRNA and protein in the non-malignant cirrhotic liver were positively correlated with tumor cell differentiation and p53 expression, which are favorable predictors of disease-specific survival.
Nianrong Zhou,Xiao He,Yuyang Yan,Xinliang Guo,Linshan Zhang,Yunhua He,Xiangyu Tan,Zhengyun Fang 한국전기전자재료학회 2022 Transactions on Electrical and Electronic Material Vol.23 No.2
The regeneration of transformer oil is a trending topic in the dielectric research field. However, the normal way of regeneration requires high cost and complicate process. In this paper, the non-woven polypropylene (PP) electret films were modifi ed by fluorination and corona method to achieve the regeneration function for transformer oil. The results show that the C-H bonds in polypropylene were replaced by C-F bonds during the fluorination process, resulting in the mean diameter of the fibers being decreased to a value of 1.7 μm. The increased roughness of the fi bers leads to super hydrophobicity. Further research revealed that the insulation properties of aging oil recovered after fi ltration by corona charged fl uorinated fi ber membranes. The result is especially signifi cant for breakdown voltage, the values of which reached half of the original insulating oil. In conclusion, the combination of fl uorinating and corona charging method on PP fi ber membrane shows great potential for insulation oil regeneration.