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신세포암종의 경부 림프절 전이 절제술 중 미주신경에 대한 수술 중 신경모니터링 증례 보고
Hyoung Shin Lee(Hyoung Shin Lee),Yeongjoon Kim(Yeongjoon Kim),Kang Dae Lee(Kang Dae Lee) 대한신경모니터링학회 2022 Journal of Neuromonitoring & Neurophysiology Vol.2 No.2
Intraoperative neuromonitoring (IONM) of recurrent laryngeal nerve has been widely applied worldwide. However, IONM of vagus nerve during lateral neck surgery has been rarely reported. In this case report, we introduce the application of IONM of vagus nerve during dissection of a metastatic lymph node of renal cell carcinoma of left level IV located deep to the vagus nerve.
Lee, Yeongjoon,Jeon, Dasom,Kim, Jin-Kyoung,Kim, Yangmee Korean Magnetic Resonance Society 2017 Journal of the Korean Magnetic Resonance Society Vol.21 No.3
Pseudin is a naturally occurring 24 amino-acid-residue antimicrobial peptide derived from the skin of paradoxical frog Pseud's paradoxa. It shows potency against the bacteria and antibiotic-resistant bacteria strain, but has high cytotoxicity against mammalian cell. In our previous study, substitution of $Pro^{11}$ for Gly (Ps-P) increased bacterial cell selectivity but decreased the antibacterial activity of pseudin. In this study, we designed pseudin analogue, Ps-4K-P with increased cationicity up to +7 in Ps-P by substituting Glu14, Gln10, Gln24, and Leu18 with Lys. Ps-4K-P showed improved potent antibacterial activity with high bacterial cell selectivity. We determined the tertiary structure of Ps-4K-P in the presence of DPC micelles by NMR spectroscopy and it has a hinge structure at $Pro^{11}$ followed by three turn helices from $Pro^{11}$ to $Val^{23}$ at the C-terminus. Amphipathicity with increased cationicity as well as helix-hinge-helix structural motif provided by introduction of a Pro at position $Gly^{11}$ are the crucial factors which confer antibacterial activity with bacterial cell selectivity to Ps-4K-P.
Lee, Yeongjoon,Kwak, Chulhee,Jeong, Ki-Woong,Durai, Prasannavenkatesh,Ryu, Kyoung-Seok,Kim, Eun-Hee,Cheong, Chaejoon,Ahn, Hee-Chul,Kim, Hak Jun,Kim, Yangmee American Chemical Society 2018 Biochemistry Vol.57 No.26
<P>Cold-shock proteins (Csps) are expressed at lower-than-optimum temperatures, and they function as RNA chaperones; however, no structural studies on psychrophilic Csps have been reported. Here, we aimed to investigate the structure and dynamics of the Csp of psychrophile <I>Colwellia psychrerythraea</I> 34H, (<I>Cp</I>-Csp). Although <I>Cp</I>-Csp shares sequence homology, common folding patterns, and motifs, including a five β-stranded barrel, with its thermophilic counterparts, its thermostability (37 °C) was markedly lower than those of other Csps. <I>Cp</I>-Csp binds heptathymidine with an affinity of 10<SUP>-7</SUP> M, thereby increasing its thermostability to 50 °C. Nuclear magnetic resonance spectroscopic analysis of the <I>Cp</I>-Csp structure and backbone dynamics revealed a flexible structure with only one salt bridge and 10 residues in the hydrophobic cavity. Notably, <I>Cp</I>-Csp contains Tyr51 instead of the conserved Phe in the hydrophobic core, and its phenolic hydroxyl group projects toward the surface. The Y51F mutation increased the stability of hydrophobic packing and may have allowed for the formation of a K3-E21 salt bridge, thereby increasing its thermostability to 43 °C. <I>Cp</I>-Csp exhibited conformational exchanges in its ribonucleoprotein motifs 1 and 2 (754 and 642 s<SUP>-1</SUP>), and heptathymidine binding markedly decreased these motions. <I>Cp-</I>Csp lacks salt bridges and has longer flexible loops and a less compact hydrophobic cavity resulting from Tyr51 compared to mesophilic and thermophilic Csps. These might explain the low thermostability of <I>Cp</I>-Csp. The conformational flexibility of <I>Cp-</I>Csp facilitates its accommodation of nucleic acids at low temperatures in polar oceans and its function as an RNA chaperone for cold adaptation.</P> [FIG OMISSION]</BR>
이하선 양성종양의 피막 외 절제술의 수술 결과 : 이하선 천엽 절제술과의 비교
김영준(Yeongjoon Kim),김창회(Chang Hoi Kim),이형신(Hyoung Shin Lee),이강대(Kang Dae Lee),김성원(Sungwon Kim) 대한두경부종양학회 2021 대한두경부 종양학회지 Vol.37 No.2
Background/Objectives: Extracapsular dissection has the advantage of reducing complications by minimizing tissue loss of the parotid without intentionally exposing the facial nerve in patients with benign parotid tumor. However, there has been controversy over the surgical results. Thus, the surgical outcomes of extracapsular dissection for benign parotid tumor was compared to those of superficial parotidectomy. Materials & Methods: A retrospective chart review was conducted with 132 patients who received surgery for benign parotid tumor in our center from January 2014 to December 2018 retrospectively. Results: A total of 132 people were enrolled, with 62 people receiving extracapsular dissection, 38 people receiving partial superficial parotidectomy and 32 people receiving superficial parotidectomy. No significant difference was found between the three groups regarding complications such as facial nerve palsy, Frey s syndrome, or first bite syndrome. Operation time and hospital stay was significantly short in extracapsular dissection group. Conclusion: For well-selected cases, extracapsular dissection can be considered as an option for surgery of benign parotid tumor.
Cheon, Dasom,Lee, Woo Cheol,Lee, Yeongjoon,Lee, Jee-Young,Kim, Yangmee Elsevier 2019 Biochemical and biophysical research communication Vol.509 No.1
<P><B>Abstract</B></P> <P> <I>Propionibacterium acnes</I> is an anaerobic gram-positive bacterium found in the niche of the sebaceous glands in the human skin, and is a causal pathogen of inflammatory skin diseases as well as periprosthetic joint infection. To gain effective control of <I>P. acnes</I>, a deeper understanding of the cellular metabolism mechanism involved in its ability to reside in this unique environment is needed. <I>P. acnes</I> exhibits typical cell membrane features of gram-positive bacteria, such as control of membrane fluidity by branched-chain fatty acids (BCFAs). Branching at the iso- or anteiso-position is achieved by incorporation of isobutyryl- or 2-methyl-butyryl-CoA via β-ketoacyl acyl carrier protein synthase (KAS III) from fatty acid synthesis. Here, we determined the crystal structure of <I>P. acnes</I> KAS III (<I>Pa</I>KAS III) at the resolution of 1.9 Å for the first time. Conformation-sensitive urea polyacrylamide gel electrophoresis and tryptophan fluorescence quenching experiments confirmed that <I>Pa</I>KAS III prefers isobutyryl-CoA as the acetyl-CoA, and the unique shape of the active site cavity complies with incorporation of branched-short chain CoAs. The determined structure clearly illustrates how BCFA synthesis is achieved in <I>P. acnes</I>. Moreover, the unique shape of the cavity required for the branched-chain primer can be invaluable in designing novel inhibitors of <I>Pa</I>KAS III and developing new specifically targeted antibiotics.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>P. acnes</I> β-ketoacyl acyl carrier protein synthase(KAS III) structure was determined. </LI> <LI> <I>P. acnes</I> KAS III prefers iosbutyryl-CoA for branched-chain fatty acid synthesis. </LI> <LI> <I>P. acnes</I> KAS III has a unique active-site cavity shape among related bacteria. </LI> <LI> This structure can help to develop novel targeted antibiotics against <I>P. acnes</I>. </LI> </UL> </P>
지반 경계조건과 설하중을 고려한 비닐온실 서까래의 구조해석 모델 개발
이상익 ( Sangik Lee ),이종혁 ( Jonghyuk Lee ),정영준 ( Yeongjoon Jung ),이승재 ( Seungjae Lee ),최원 ( Won Choi ) 한국농공학회 2019 한국농공학회 학술대회초록집 Vol.2019 No.-
비닐온실은 우리나라에서 널리 이용되고 있는 시설재배방법이지만 겨울철 폭설로 인해 지속적인 피해가 발생하고 있다. 안전 적설심에 대한 설계 기준이 제시되고 있음에도, 기존의 설계 기준은 온실 서까래가 설치되는 경계조건을 충분히 반영하지 못하고 있다. 겨울철 낮과 밤의 온도 차로 인한 지반의 반복적인 융해 및 동결로 지반은 그 경계조건이 고정이 아닌 힌지에 가까운 상태로 변하게 되어 온실의 서까래 파이프를 충분히 지지하지 못하게 된다. 그뿐만 아니라 실제 온실의 설치에 있어 시공의 편의를 위해 설계도에 있는 콘크리트 기초 혹은 줄기초를 설치하지 않는 경우가 있다. 따라서 설하중이 작용할 때, 그 경계지점이 회전하거나 이동하여 경계조건이 변화하게 된다. 본 연구에서는 이를 반영하기 위해 적설심별 설하중에 따라 지반에서 발생하는 반력과 모멘트를 고려하여 지반에 관입된 파이프의 재하실험을 진행하고, 이를 통해 얻은 경계조건의 변화를 적용할 수 있는 수치해석 기반의 온실 구조해석 모델을 개발한다. 실험을 통해 얻은 경계지점의 변위와 회전은 수치해석 모델에서 지정된 변위 및 회전으로 적용되어 지반 경계조건을 고려한 온실의 구조해석을 진행한다. 또한, 기존의 해석방법인 고정 혹은 힌지 경계조건을 적용한 구조해석을 통해 산정된 최대 휨모멘트 및 안전 적설심과 개발된 구조해석 모델을 통해 산정된 결과를 비교한다. 이를 통해 설하중이 작용하는 온실의 지반 조건을 고려한 실제 거동과 구조적 안정성을 분석하고, 증가하는 이상기후에 따른 기상재해 예방에 기여할 수 있을 것으로 기대한다.
스마트폰을 이용한 실시간 혈압측정 시스템의 설계 및 구현
길영준(Yeongjoon Gil),이정태(Jungtae Lee) 한국정보과학회 2015 정보과학회 컴퓨팅의 실제 논문지 Vol.21 No.3
ECG, PTT 및 SpO₂와 같은 생체신호를 이용하여 실시간으로 혈압을 측정하고, IPv4/IPv6프로토콜을 이용하여 인터넷에 접속할 수 있는 시스템을 설계하고 구현하였다. 아울러 임상 실험을 통해 실시간 환경에서 수축기 혈압과 이완기 혈압을 계산할 수 있는 모델을 제시하였다. 이렇게 구현된 실시간 혈압측정 시스템을 28명의 피험자를 대상으로 임상 실험을 실시한 결과, 영국 고혈압협회(BHS)에서 인증하는 혈압계 인증 등급 Grade B 수준에서 정상으로 동작함을 확인하였다. Blood pressure may be measured using physiological signals, such as ECG, PTT, and SpO₂. In this study, we designed and implemented a portable real-time blood pressure measuring system, which can access the Internet simultaneously using the IPv4 / IPv6 protocol. In addition, we presented a model for the calculation of systolic and diastolic blood pressure in a real-time environment. Through clinical experiments on 28 subjects, the blood pressure measuring system was shown to satisfy the requirements of Grade B level of the BHS (British Hypertension Society) certification.
( Prasannavenkatesh Durai ),( Yeongjoon Lee ),( Jieun Kim ),( Dasom Jeon ),( Yangmee Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.5
Papiliocin, isolated from the swallowtail butterfly (Papilio xuthus), is an antimicrobial peptide with high selectivity against gram-negative bacteria. We previously showed that the N-terminal helix of papiliocin (PapN) plays a key role in the antibacterial and anti-inflammatory activity of papiliocin. In this study, we measured the selectivity of PapN against multidrug-resistant gram-negative bacteria, as well as its anti-inflammatory activity. Interactions between Trp2 of PapN and lipopolysaccharide (LPS), which is a major component of the outer membrane of gram-negative bacteria, were studied using the Trp fluorescence blue shift and quenching in LPS micelles. Furthermore, using circular dichroism, we investigated the interactions between PapN and LPS, showing that LPS plays critical roles in peptide folding. Our results demonstrated that Trp2 in PapN was buried deep in the negatively charged LPS, and Trp2 induced the α-helical structure of PapN. Importantly, docking studies determined that predominant electrostatic interactions of positively charged arginine residues in PapN with phosphate head groups of LPS were key factors for binding. Similarly, hydrophobic interactions by aromatic residues of PapN with fatty acid chains in LPS were also significant for binding. These results may facilitate the development of peptide antibiotics with anti-inflammatory activity.