산채류 추출물이 세균의 성장에 미치는 영향(Ⅰ)

지원대,이종만,정영건 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1

본 실험은 일반 가정의 식탁에서 흔히 볼 수 있는 산채류 20종에 대해 3주의 gram 양성균(Bacillus subtilis, Lactobacillus casei, Staphylococcus aureus)및 2주의 gram음성균(Klebsie-lla pneumoniae, Shigella sp.)의 성장에 미치는 영향을 조사하였다. 본 실험에 사용한 대부분의 산채류 추출액은 공시균의 성장을 다소 증가시켰다. 특히 표고버섯과 고사리는 그람양성균인 B.subtilis, L.casei, S.aureus균의 취, 더덕과 토란은 B.subtilis, S. aureus균의 성장을 대조군에 비해 2~6배로 크게 증가시켰다. 그러나 그람음성균인 K.pneumoniae와 Shigella sp.에 대해서는 성장을 크게 증가시키는 시료는 없었다. 한편, 죽순은 B.subtilis의, 부지깽이와 민들레는 L.casei의, 팽이버섯과 돌나물 및 두릅은 S. aureus의, 산초, 다래순, 두릅 및 마는 K. pneumoniae의, 죽순, 두릅 및 마는 Shigella sp.의 생육을 다소 저해하는 것으로 나타났으나 항균력은 인정할 수 없었다. This study was carried out to investigate how to influence mountainvegetables, which are commonly available in high mountains in Korea, on bacte-rial growth. Total 20 kinds of mountain vegetables and mushrooms were sampledand after boiling them in water, put water extracts of them into the media prepa-red for the smear of test strains, which were included three of Gram positivestrains (Bacillus subtilis, Lactobacillus casei and Staphylococcus aureus) andtwo of Gram negative strains(Klebsiella pneumoniae and Shigella sp.). The gro-wth of all strains were more or less increased in the media injected with waterextracted samples than that of the control. Especially the growth of B. subtilis,L. casei and S. aureus were increased by the water extracts of Pyogo mushroomand Bracke and the samples from Aster, Deodeok(Codonopsis lanceolata) andTaro were appeared with the increase in growth of B, subtilis and S. aureusby two to six times increase rate than that of control. However, the more growthincrease of K. pneumoniae and Shigella sp. were not found than that of control.There were also observed some of water extracted samples have slight inhibi-tions to the bacterial growth. The growth of strains inhibited by the sampleswere as follows .B. subtilis by water extracts of Bamboo shoot, L. casei bythem of Busiggaengi(Erysimum aurantiacum) and Dandelion, S. aureus by themof Pengi mushroom, Sedum and Fatsia, K. pneumoniae by of them of Chinesepepper, Darae(Actinidia arguta) , Fatsia and Yam and Shigella sp. by of themof Bamboo shoot. Fatsia and Yam. However. these antibiotic effects were soweak that scarecely proved their effects.

Analysis of acne-related judicial precedents from 1997 to 2018 in South Korea

( Ji Hoon Yang ),( Soo Ick Cho ),( Su Hwan Shin ),( Won Lee ),( So Yoon Kim ),( Dae Hun Suh ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2

Background: Medicolegal disputes are increasing in practical medicine. Acne is a common problem but is usually related to cosmetic problems such as acne scars. Medications or procedures related to acne could lead to medical malpractice. Objectives: To analyze medical litigation associated with acne or acne scar in South Korea. Methods: Acne-related judgements were searched using the Supreme Court of Korea’s Written Judgment Management System based on the keywords “acne” or “acne scar.” Results: Eleven cases were selected as litigated cases of acne or acne scar. Eight cases (72.7%) were related to acne scar and three (27.3%) were related to acne. Treatment modalities such as peeling (n = 6), laser treatment (n = 3), photodynamic therapy (n = 1), and antibiotic (n = 1) resulted in lawsuit. Claimed sequelae of the treatment were hyperpigmentation (n = 5), scar worsening (n = 5), erythema (n = 3), skin bumps (n = 1), and liver transplant (n = 1). Eight cases (72.7%) were awarded to the plaintiff, and the others were dismissed. The average awarded amount was 16,801,324± 24,452,486 (mean±standard deviation) Korean Won. Conclusion: Various treatments for acne or acne scar can cause medical disputes. Unnecessary litigation could be prevented if simple measures such as history taking, choosing proper procedure, and adequate management after the procedure along with sufficient informed consent were performed.

항결핵 치료과정에서 호전되었다가 악화된 뇌 결핵종 1예 보고

이대훈,유진홍,강미자,김홍기,김선화,고동훈,천지성,유원종 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.3

In spite of nationmide tuberculosis control program, tuberculosis is still prevalent in Korea. Accordingly, tuberculous meningitis also poses one of the main trouble some problems. Recently, We experienced a perplexing case of tuberculous menin-gitis and tubercdoma in a 24-year old female patient. She was admitted to our hospital owing to meningeal irritation sign, aphasia, VI nerve palsy, and ,fever. CSF analysis showed a finding consistent with that of presumptive tuberculous meningitis.. Initial MRI of the brain revealed a large mass(tuhercu1oma) on left parieto-temporal area. Antitubeculous regimen(1soniazid (INH), rifampin(RMP), ethambutol(EMB), pyrazinamide(PZA)} of meningeal dose was given immediately, and her symptoms and signs were dramatically disappeared with a few days after treatment. She was discharged with improved condition and under maintenance treatment of tuberculosis. Follow-up one year after treatment. revealed that the previous lesion was nearly diminished. She showed no symptom at all until she recognized a total blindness of sudden onset on her left eye a t the 18th month of treatment. Brain MRI checked at that time showed multiple masses of tuberculoma scattered over the brain base and one of them invaded left optic nerve. In our opinion, the cause of relapse in this patient might he explained by the selection and ultimate replication of a minor population of resistant strain. Although such relapse is an extremely rare event, we think that we should always pay attention to this possible resistance problem every time we meet patients with tuberculous meningitis.

바이러스 감염 후 발생한 소아에서의 시선경염 1례

신지원,김영대,김우경,박용원,오인경 인제대학교 2009 仁濟醫學 Vol.30 No.-

Optic neuritis presents differently in children than in adults. Children typically have bilateral involvement with papillitis following an antecedent viral illness. The development of multiple sclerosis is less common in children. The diagnosis of optic neuritis is usually made on a clinical manifestations. The major symptoms of optic neuritis include one or more of the following: 1) sudden blurred, "foggy" vision and sudden loss of vision, 2) pain with eye movement in the affected eye, 3) sudden color blindness, and etc. This condition progresses over a few days to two weeks. A 12-year-old girl presented with a history of sudden visual loss and color blindness. Treatment with high doses of steroid resulted in visual function returning to normal.

연골막판으로 싼 정제된 산호의 조직학적 연구

김지수,손대구,한기환,최동원,박관규 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

Autogenous costal cartilage graft has been commonly used for reconstruction of auricular deformity. However, the risk of complication and discomfort at the donor site, as well as distortion of the graft due to morphological change in the cartilage have been serious drawbacks to this procedure. Previous studies examining the chondrogenic potential of perichondrium have suggested that perichondrium may be used as graft for cartilage reconstruction. When a perichondrial flap or a free perichondrium was used as graft, new cartilage formed appositional to the grafted perichondrium. However, the neocartilage was often irregular in shape and varied considerably in quantity. In this study, the feasibility of controlling the shape and the mass of neocartilage was investigated using coral, a porous biomaterial, as a template. A coral a template was wrapped with perichondrial flap from the ears of New Zealand white rabbits and placed into a subcutaneous pocket in the rabbits and placed into a subcutaneous pocket in the rabbit's back by incision. A total of 12 animals were used. Formation of new cartilage was later evaluated by gross and histological examination of the perichondrial flap and the coral template. New cartilage was formed in 11 animals. Immature chondrocytes were visible by 3 weeks after the surgery, and by 8 weeks the immature chondrocytes had formed a cartilage. New cartilage was formed only on the surface of the coral template. These results indicated that the shape and the mass of new cartilage may be controlled by using coral template. Therefore, the desired shape of cartilage may be achieved using a coral template of corresponding shape, and this may help in correcting subtle auricular contour defect and in correcting other structural defects that also require new cartilage formation.

Bifidobacterium angulatum KCTC3236의 α-galactosidase에 의한 Oligo당 생성의 최적조건

왕지원,김경민,강대중,강국희,정재록 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

Bifidobacterium angulatum KCTC3236 showed the highest α-galactosidase activity among the tested strains of Bifidobacteria( data not shown). Optimum culture conditions for the maximum production of α-galactosidase were as follows. The culture media was MRS broth containing 1% galactose as a carbon source and its initial pH was 7.0. The incubation temperature and time were 37℃, 21 hours respectively. Melibiose have been used as substrate. The formation of oligosaccharides was maximum at 40℃ when the substrate concentration was 30%. And the unit of α-galactosidase was 40 unit. Finally, we could observe the maximum production of oligosaccharides after 14 hours' enzyme reaction.

Flavobacterium meningosepticum에 의한 균혈증 1예

장성원,유진홍,진승원,김선우,김연식,박순민,이대훈,최민호,최주연,신완식,강문원,강지민 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.1

1996년 6월 카톨릭대학교 부천성가병원에 뇌경색으로 입원했던 61세 남자 환자의 혈액에서 Flavobacterium meningosepticum을 분리하였다. 환자는 폐렴을 동반한 패혈증의 예로 ceftriaxone과 metronidazole을 투여하였으나 입원 48시간만에 사망하였다. Flabovacterium meningosepticum is a rae cause of nosocomial infection which shows multi-drug resistance. It mainly invades patients with impaired immunity. Recently, we experienced a case of F. meningosepticum bacteremia in a patient with chronic debilitated state owing to stroke. The clinical progress was so rapid that he died within 48 hours. Blood culture revealed F. meningosepticum which showed characteristic yellow colony in blood agar plate.

미생물을 이용한 염료의 생분해에 관한 연구 : 반응성염료 분해균에 관한 연구 Studies on the Reactive Dye-Degrading Microorganisms

정영건,지원대,정민선,백형진 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1

대구시의 염색폐수처리장 폐수 및 처리장 주변의 토양과 물에서 반응성 염료인 Cibacron Brilliant Red 3B-A(CBR), Reactive Orange 16(RO), Reactive Black 5(RB)에 탈색능을 보이는 12종의 균을 분리하고 이들 균 중에서 본 실험에 사용한 CBR, RO, RB5 염료 모두에 약 90% 이상의 높은 탈색율을 나타낸 CBR2균을 선별하였다. 선별된 CBR2균의 특성 및 염료분해특성을 조사하였다. CBR2균은 그람 음성, 무아포, 간균으로 운동성이 있으며, 염농도 5%, 온도 50℃에서도 생육이 가능하였다. CBR2균의 생육 및 염료의 탈색에 미치는 pH의 영향을 조사한 결과, pH 5.0에서 9.0까지 고른 균 성장과 탈색율을 나타내었으며 특히 pH 7.0에서 최고의 균 성장 및 염료 탈색율을 나타내었다. 온도별 균의 생육 및 염료의 탈색율을 조사한 결과 30℃에서 가장 높은 균 성장 및 탈색율을 나타내었다. 산소량에 따른 균의 생육 및 염료의 탈색율을 조사한 결과, 산소량이 많을수록 균의 생육이 좋았으며, 염료의 탈색율에 있어서도 CBR 염료의 경우는 산소량에 비례해서 탈색율이 좋았으나, RO와 RB5 염료의 경우는 산소량이 가장 많은 2ml양의 배양에서 보다 상대적으로 산소량이 적은 4ml양의 배양에서 가장 높은 탈색율을 보였다. Twelve strains, which were exhibited abilities of decolorizationto the reactive dyes, Cibacron Brilliant Red 3B-A(CBR) , Reactive Orange 16(RO) and Reactive Black 5(RB5) , were isolated from the samples of waste waterof the dye textile factories and the samples of soil and water around them. Amongthem one stain, CBR2 was selected because of the higest decolorization rateto the reactive dyes, CBR, RO and RB5 with more than 90% of decolorizationrate. The characteristics of reactive dye-degrading microorganism CBR2 andits decolorization abilities to reactive dyes were investigated. This CBR2 wasGram negative and non-spore forming rod. This organism has motility and ispossible to grow even in 5% of sodium chloride solution and at such temperatueof 50℃ In the pH 5.0 to 9.0, growth rate of the isolate and decolorizationrate to reactive dyes by CBR2 were exhibited almost similar rates. However,among those pH of media the most growth and decolorization rates were appearedat pH 7.0. On the temperature effects to growth rates of CBR2 and decolorizationrate by this organism, the most growth and decolorization rates were at tempera-ture of 30℃. More oxygen volumes in test tubes enhanced generally the growthrate of this organism. However, the decolorization rate of CBR dye by CBR2was higher according to oxygen volume increased in the media but the decoloriza-tion rates of RO and RB5 dyes in 2m1 volume of culture media were rather decrea-sed than that of 4ml volume of culture media where oxygen volume is lowerthan that of 2ml volum of culture media.

석면폐증 1례

이영수,장태원,유호대,정만홍,이용환,서지영,허 방,이재성 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.1

Asbestosis is the disease of pulmonary fibrosis caused by the inhaled asbestos fibers, and could be diagnosed clinically, in the case of exposure history to asbestos is proved, by clinical symptoms of dyspnea or dry cough, physical examonation findings, and the radiographic features. But many othder inorganic dusts would show similar findings in the chest radiogram and sometimes the exposure history is obscure, so for the exact diagnosis of asbestosis lung biopsy is needed. In Korea, there have been some reports of survey in the workplace where asbestos is handled or of asbestos related diseases. This is a case report of asbestosis with accompanying pleural plaques, who had the occupational exposure to asbestos for 30 years and the consistent clinical, radiographic and pathological findings in the lung tissue obtained by the videoscope assisted thoracoscopic biopsy(VATS).

볏짚으로 제조한 청국장의 특성

孫東和,梁成鎬,池元大 대구산업정보대학 1998 논문집 Vol.12 No.2

This study was conducted to investigate main odor components of Chungkook-jang fermented with organisms in rice straw. In order to search major aroma components, changes of aroma compounds and sensory score were compared for fermentation period. 30 aroma compounds were identified as a result of analysis using GC-MSD. Aroma of Chungkook-jang was leaded by 5 compounds, 2,5-dimethyl-pyrazine, 2,3-dimethyl-5- ethylpyrazine, trimethylpyrazine, tetramethylpyrazine and 3,5-diethyl-2- methyl-pyrazine. Major aroma components of Chungkook-jang on aging time were studied on the basic of sensory score. The desirable odor components were showed as 1-octen-3-ol and guaiacol. The undesirable odor components were appeared as butanoic acid and 3-methyl-butanoic acid