Identification and characterization of ERRFI1 in chickens

Suzie E. Ahn,Whasun Lim,Wooyoung Jeong,Jihye Kim,Jinyoung Kim,Jin Won Choi,Jae-Wook Jeong,Jae Yong Han,Gwonhwa Song 한국가금학회 2010 한국가금학회 정기총회 및 학술발표회 Vol.27 No.-

ERBB receptor feedback inhibitor 1 (ERRFI1, also known as MIG-6) is a negative regulator of epidermal growth factor signaling pathway in various biological processes. However, the identification and characterization of ERRFI1 gene in chickens has not been reported. In the present study, we investigated ERRFI1 gene expression in each embryonic stage and adult organs by RT-PCR, quantitative RT-PCR, in situ hybridization and immunohistochemical analyses. Our current data showed that the ERRFI1 mRNA was expressed in brain, kidney, small intestine and muscle of male embryo, and weakly expressed in kidney, heart, liver and muscle of female embryo. In adult tissues, ERRFI1 is specifically expressed in seminiferous tubules in the testis and glandular epithelium in the oviduct after sexual maturation. Immunohistochemistry revealed that ERRFI1 protein was particularly abundant in the nuclei of luminal and glandular epithelia in the oviduct. In addition, treatment with synthetic estrogen analog diethylstilbestrol (DES) stimulated ERRFI1 expression in the oviduct. Collectively, the present studies validate ERRFI1 as a novel estrogen-stimulated gene in the oviductal epithelia in chickens.

자영업자와 임금근로자의 의료 이용 차이: 시간의 기회비용 관점에서

안수지 ( Suzie Ahn ) 한국경제학회 2020 經濟學硏究 Vol.68 No.4

본 연구는 우리나라의 자영업자가 정규직 및 상용직 임금근로자에 비하여 의료이용이 제한되고 있으며, 사회경제적 특성에 따른 기회비용의 차이가 그 주요한 원인임을 밝힌다. 일반적으로 소득이 높을수록 의료이용이 증가하는 양의 소득효과가, 정규직 및 상용직 임금근로자에 비해 자영업자에게서 유의하게 작은 것으로 나타났다. 이는 자영업자의 경우 근로 시간이 소득과 직결되는 특성으로 인해 의료이용에 따른 기회비용이 임금근로자에 비해 높다는 점에 기인한다. 이와 유사하게, 임금근로자라 하더라도 유급휴가가 적은, 즉 의료이용에 따른 기회비용이 상대적으로 큰 경우로 한정하면 자영업자와 비슷한 의료이용 패턴을 보였으며, 자영업자 중에서도 배우자가 함께 자영업에 종사하여 상호 보완적인 역할을 할 수 있는 경우에는 임금근로자와 다르지 않은 의료이용을 나타냄을 확인하여, 기회비용의 차이가 의료이용 격차를 발생시키고 있음을 일관되게 보여주었다. This study shows that the difference in opportunity costs between self-employed and permanent wage-and-salary workers is one of the main factors contributing to socioeconomic inequalities in health care utilization. The positive income effect on health care utilization is shown to be significantly smaller for self-employed than for wage-and-salary workers. Since working hours are directly related to the income for self-employed workers, it is more costly for them to use health care services at the expense of working hours than for wage-and-salary workers. For similar reasons, even among wage-and-salary workers, those who lack access to paid leave tend to reduce their health care utilization as self-employed workers do. Furthermore, I investigate the changes in patterns of health care utilization for self-employed workers whose spouses are also self-employed so that the loss of working hours can be somewhat mitigated, and find that there are no significant differences in the use of health care services between those and wage-and-salary workers.

Paclitaxel 매주 투여 및 방사선치료 동시요법을 받은 국소진행성 비소세포폐암 환자들의 치료 결과

김수지(Suzy Kim),김성환(Sung Whan Kim),심병용(Byoung Yong Shim),김치홍(Chi Hong Kim),송소향(So Hyang Song),안명임(Meyung Im Ahn),조덕곤(Deog Gon Cho),조규도(Kyu Do Cho),유진영(Jinyoung Yoo),김훈교(Hoon Kyo Kim) 대한방사선종양학회 2006 Radiation Oncology Journal Vol.24 No.4

목 적: 국소진행성 비소세포폐암 환자에 대한 매주 paclitaxel 항암화학요법과 방사선치료 동시 요법의 안정성과 효 과를 알아보고 재발 양상 및 생존율을 분석하고자 하였다. 대상 및 방법: 1999년 10월부터 2004년 9월까지 국소진행성 비소세포폐암으로 진단받고 근치적 목적으로 항암화 학방사선 동시요법을 시행 받은 환자 23명을 대상으로 후향적 분석을 시행하였다. 방사선치료는 일일 1회 1.8 Gy 씩 주5회 분할 조사하여 7∼8주에 걸쳐 총 선량 55.8∼64.8 (median 64.8) Gy를 조사하였다. 항암화학요법은 매주 paclitaxel 50 또는 60 mg/m2 용량으로 방사선치료 1일, 8일, 15일, 22일, 29일 36일째에 투여하였다. 항암화학방사선동시요법을 마친 4주 후부터 paclitaxel 135 mg/m2와 cisplatin 75 mg/m2 용량으로 3주 간격으로 3주기의 공고 항암화학요법을 추가 시행하였다. 결 과: 동시 항암화학방사선요법을 시행받은 23명의 환자 중 3명이 도중에 환자 임의로 치료를 중단하였고 1명이 5,580 cGy까지 방사선치료를 시행 받고 세균성 폐렴으로 사망하였다. 주된 급성 부작용은 방사선 식도염으로 4명(17%)의 환자에서 2도의 식도염이 관찰되었으며 3도 이상의 부작용은 관찰되지 않았다. 16명의 환자가 추가 공고항암화학요법을 시행 받았으며 공고 항암화학요법 중의 급성 부작용으로 3도 이상의 호중구 감소증이 8명(50%)의 환자에서 관찰되었으며 그중 한 명은 패혈증으로 사망하였다. 동시 항암화학방사선요법을 끝까지 시행 받은 20명의 환자에서 치료에 대한 반응을 조사할 수 있었으며 완전 관해 4명(20%), 부분 관해 14명(70%)으로 전체 관해율은 90%이었다. 관해를 보인 환자들 중 추적 관찰이 가능했던 16명 중 14명에서 재발이 확인되었고 국소 재발이 9명(56%), 국소 재발과 원격 전이가 3명(19%), 원격 전이가 2명(13%)이었다. 동시 항암화학방사선요법을 끝까지 시행받은 환자들에서의 무진행 생존 기간의 중앙값은 9.5개월이었으며, 2년 무진행 생존율은 18%이었다. 재발된 환자중 11명에서 2차(second-line) 또는 3차(third-line) 항암화학요법이 시행되었다. 전체 환자 23명의 중앙 생존 기간은 21개월, 2년 및 5년 생존율은 각각 43%, 33%였다. 다변량 분석을 시행했을 때 환자의 나이, 수행 능력, 종양의 크기는 무진행 생존율에 영향을 주는 유의한 예후 인자로 나타났다. 결 론: 국소진행성 비소세포폐암 환자에서 paclitaxel 매주 투여 항암화학요법과 방사선치료 동시요법은 안전하고 종양의 관해율도 높았다. 그러나 국소 재발률이 높고 특히 종양의 크기가 큰 환자에서 예후가 나쁜 것을 알 수 있었다. 따라서 향후 부작용은 증가시키지 않으면서 국소제어율을 향상시키기 위한 노력이 필요하다. Purpose: To analyze the response, toxicity, patterns of failure and survival rate of patients with locally advanced non-small cell lung cancer who were treated with concurrent chemoradiotherapy with weekly paclitaxel. Materials and Methods: Twenty-three patients with locally advanced non-small cell lung cancer patients who received radical chemoradiotherapy from October 1999 to September 2004 were included in this retrospective study. Patients received total 55.4∼64.8 (median 64.8) Gy (daily 1.8 Gy per fraction, 5 days per weeks) over 7∼8 weeks. 50 or 60 mg/m2 of paclitaxel was administered on day 1, 8, 15, 22, 29 and 36 of radiotherapy. Four weeks after the concurrent chemoradiotherapy, three cycles of consolidation chemotherapy consisted of aclitaxel 135 mg/m2 and cisplatin 75 mg/m2 was administered every 3 weeks. Results: Of the 23 patients, 3 patients refused to receive the treatment during the concurrent chemoradiotherapy. One patient died of bacterial eumonia during the concurrent chemoradiotherapy. Grade 2 radiation esophagitis was observed in 4 patients (17%). Sixteen patients received consolidation chemotherapy. During the consolidation chemotherapy, 8 patients (50%) experienced grade 3 or 4 neutropenia and one of those patients died of neutropenic sepsis. Overall response rate for 20 evaluable patients was 90% including 4 complete responses (20%) and 14 partial responses (70%). Among 18 responders, 9 had local failure, 3 had local and distant failure and 2 had distant failure only. Median progression-free survival time was 9.5 months and 2-year progression-free survival rate was 19%. Eleven patients received second-line or third-line chemotherapy after the treatment failure. The median overall survival time was 21 months. 2-year and 5-year survival rate were 43% and 33%, respectively. Age, performance status, tumor size were significant prognostic factors for progression-free survival. Conclusion: Concurrent chemoradiotherapy with weekly paclitaxel revealed high response rate and low toxicity rate. But local failure occurred frequently after the remission and large tumor size was a poor prognostic factor. Further investigations are needed to improve the local control.

Identification and Regulation of AHCYL1 Gene by Estrogen in Chickens

Wooyoung Jeong,Suzie E. Ahn,Jinyoung Kim,Fuller W. Bazer,Jae Yong Han,Gwonhwa Song 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1), also known as IP3 receptor- binding protein released with IP3 (IRBIT), is a member of the AHCY-like protein family. AHCYL1 protein regulates IP3-induced Ca2+ release in the cytoplasm of cells and, therefore, is likely to be an important gene regulating various biological processes in the oviduct of chickens. Inmammals, expression is greatest during activation of dendritic cells which are antigen presenting cells associated with immunoregulatory processes in blood and skin. However, the identification of the AHCYL1 gene in chickens has not been investigated. In the present study, we first used RT-PCR to demonstrate AHCYL1 gene expression in adult chicken organs and oviducts of immature chickens treated with DES (diethylstilbesterol, a synthetic estrogen agonist). The results indicated that AHCYL1 mRNA is expressed in chicken reproductive organs (testis, ovary and oviduct). Inaddition, expression of AHCYL1 mRNA increased in response to DES-treated immature oviducts compared to the non-treated control immature oviducts of chickens. Interestingly, AHCYL1 was abundant in the cytoplasm of luminal and glandular epithelia, but not in other cell-types such as stroma and connective tissues, of the chicken oviduct. These results suggest that AHCYL1 is a novel estrogen-stimulated gene associated with development of the chicken oviduct, as well as functions of oviductal glandular and luminal epithelia that may include activation of resident immune cells, such as dendritic cells.

Matrix metalloproteinase 3 is a stromal marker for chicken ovarian cancer

CHOI, JIN WON,AHN, SUZIE E.,RENGARAJ, DEIVENDRAN,SEO, HEE WON,LIM, WHASUN,SONG, GWONHWA,HAN, JAE YONG Spandidos Publications 2011 Oncology letters Vol.2 No.6

<P>Matrix metalloproteinases (MMPs) are involved in the degradation of the extracellular matrix and basement membranes. Due to this, MMPs have been thought to promote invasion and metastasis of cancer cells and angiogenesis in tumors. Even though the chicken is a useful animal model for studying human ovarian cancer, no reports exist of the MMP expression pattern in chicken ovarian cancer. Therefore, we investigated the expression pattern of MMPs in chicken ovarian cancer. Results of RT-PCR and quantitative RT-PCR analyses showed MMP3 to be over-expressed in cancerous hen ovaries. In situ hybridization analysis of cancerous chicken ovaries showed that MMP3 mRNA was predominantly localized in the stroma, which is similar to MMP3 expression in human cancers. The results suggest that the expression pattern of MMP3 mRNA in chicken ovarian cancer is similar to that in various types of human cancer. Moreover, MMP3 potentially plays a significant role in developing ovarian cancer in chickens. The cell type-specific expression of MMP3 makes this gene a unique marker for ovarian cancer in chickens.</P>

Estrogen Regulates ERRFI1 Expression during Development of the Chicken Oviduct

Chul-Hong Lim(임철홍),Suzie E. Ahn,Whasun Lim,Wooyoung Jeong,Jinyoung Kim,Fuller W.Bazer,Jae Yong Han,Gwonhwa Song 한국가금학회 2011 한국가금학회 정기총회 및 학술발표회 Vol.28 No.-

S-adenosylhomocysteine Hydrolase-like Protein 1 (AHCYL1) is Mediated by Estrogen-induced ERK1/2 MAPK Cell Signaling and microRNA Regulation to Effect Functional aspects of the Avian Oviduct

Wooyoung Jeong,JinyoungKim,SuzieE.Ahn,WhasunLim,Chulhong Lim,Jinyoung Lee,Seungmin Bae,FullerW.Bazer,GwonhwaSong 한국동물번식학회 2012 Reproductive & developmental biology Vol.36 No.2

S-adenosylhomocysteine Hydrolase-like Protein 1 (AHCYL1) is Mediated by Estrogen-induced ERK1/2 MAPK Cell Signaling and microRNA Regulation to Effect Functional aspects of the Avian Oviduct

Wooyoung Jeong,JinyoungKim,SuzieE.Ahn,WhasunLim,Chulhong Lim,Jinyoung Lee,Seungmin Bae,FullerW.Bazer,GwonhwaSong 한국동물번식학회 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s

S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1), also known as IP3 receptor- binding protein released with IP3 (IRBIT), regulates IP3-induced Ca2+ release in the cytoplasm of cells and, therefore, is likely to be an important gene regulating various biological processes in the oviduct of chickens. However, the identification of the AHCYL1 gene in chickens has not been investigated. Therefore, the objectives of this study were to examine the tissue- and cell-specific expression of AHCYL1 gene in chicken organs, especially in reproductive organ, and determine functional actions of AHCYL1 in chicken oviduct development via estrogen. The results indicated that AHCYL1 mRNA is expressed in chicken reproductive organs and DES(diethylstilbesterol, a synthetic estrogen agonist) stimulates the cell specific expression of AHCYL1 in immature chicken oviduct. These results suggest that AHCYL1 is a novel estrogen-stimulated gene associated with development of the chicken oviduct. Next, in the present study, we show that inhibition of Erk1/2 can block DES-induced AHCYL1 expression. Also, we found that knockdown of AHCYL1 expression down-regulates expression of oviduct specific genes and AHCYL1 expression is regulated at the post-transcriptional level by specific miRNAs. These results strongly suggest that estrogen-mediated AHCYL1 gene expression plays a crucial role in growth, differentiation and function of the hen oviduct. Also, our results will be useful for understanding the fundamental mechanism(s) of estrogen action responsible for development of hen oviduct. This research was funded by the World Class University (WCU) program (R31-10056), Basic Science Research Program (2010-0013078) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology and by the Next-Generation BioGreen 21 Program (No.PJ008142), Rural Development Administration, Republic of Korea.

Chicken SERPINBs: Characteristics, Tissue Specific Expression and Regulation of Expression by Estrogen

Whasun Lim,Jee-Hye Kim,Suzie E. Ahn,Wooyoung Jeong,Jinyoung Kim,Jae Yong Han,Fuller W. Bazer,Gwonhwa Song 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Serpins are a superfamily of related protease inhibitors with common structural features and inhibitory mechanisms. However, SERPINA 14 in mammals does not have inhibitory activity against most known proteases. Rather, it may have an immunoregulatory role in mammals to prevent rejection of the fetal allograft by inhibiting lymphocyte proliferation and natural killer cell function. In the pig, SERPINA14 is involved in iron transport to the fetus by binding to and stabilizing the iron-binding protein uteroferrin (ACP5). In chickens, these very little known about serpins in chickens. Therefore, we investigated the expression patterns of serpin genes in the oviduct of adult hens and in the oviduct of 37-day-old chicks treated with an estrogen analogue, diethylstilbestrol (DES). Results indicated that SERPINB3 and SERPINB11 genes were highly expressed in oviducts of DES-treated chicks, but not in oviducts of control chicks. Both SERPINB3 and SERPINB11 transcripts were localized specifically to the gland-like areas of oviducts of DES-treated chicks. Immunohistochemical analyses confirmed that SERPINB3 and SERPINB11 proteins were present in the gland-like area and luminal epithelium of the oviducts of DES-treated chicks. Collectively, the results suggest that SERPINB3 and SERPINB11 are expressed in response to estrogens and they have distinct functions related to development and differentiation of the mature oviduct in hens.

Cell-specific and temporal aspects of gene expression in the chicken oviduct at different stages of the laying cycle.

Jeong, Wooyoung,Lim, Whasun,Kim, Jinyoung,Ahn, Suzie E,Lee, Hyung Chul,Jeong, Jae-Wook,Han, Jae Yong,Song, Gwonhwa,Bazer, Fuller W Society for the Study of Reproduction [etc.] 2012 BIOLOGY OF REPRODUCTION Vol.86 No.6

<P>Egg formation and embryonic development occur as the yolk passes through the magnum, isthmus, and shell gland of the oviduct before oviposition in hens. The present study identified candidate genes associated with secretory function of the chicken oviduct after ovulation and contributing to egg formation and oviposition. Hens (n = 5 per time point) were euthanized to recover the reproductive tract when the egg was in the magnum (3 h after ovulation) and the shell gland (20 h after ovulation). Total RNA was extracted from each segment of the oviducts and subjected to Affymetrix chicken GeneChip analysis. Quantitative PCR and in situ hybridization analyses of selected genes confirmed the validity of the gene expression patterns detected using microarray analysis. In particular, ACP1, CALB1, CYP26A1, PENK, RCAN1 and SPP1 expression increased significantly in the shell gland between 3 h and 20 h postovulation, whereas only RCNA1 expression increased significantly in the magnum between 3 h and 20 h postovulation. Results of the high-throughput analysis revealed cell-specific and temporal changes in gene expression in the oviduct at 3 h and 20 h postovulation in laying hens provide novel insight into changes at the molecular and cellular levels of candidate genes related to formation of the egg and oviposition.</P>