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강진한,허재균,김종현,이경일,박수은,마상혁,이명숙,반상자,홍성화,조대현,이성호 대한감염학회 2001 감염 Vol.33 No.2
Background : The incidence of tetanus in adults and neonatal tetanus have been markedly reduced by world-wide use of DTP vaccines. But, tetanus is still one kind of major health problems in many developing countries, and several serosurvey stduies in developed ountries revealed that substantial proportions of adult opulation may lack immunity against tetanus and imunity level against tetanus is continuously decreasing by age. In Korea, tetanus outbreaks have been disappeared since the 1980s by high acceptant DTaP vaccination rates. Annually, few tetanus patient has been reported since 1990s. But, there knave been no seroepide-miological studies to tetanus, no trials to assess tetanuts immunizations. And we do not use Td vaccine in adults for maintaning tetanus immunity. In this aspect, we conducted age related survey of immunity to tetanus and indirectly assessed the immunogenecity of tetanus vaccines, used in Korea. Methods : For the evaluation of age related sero-survey of tetanus immunity in Korean population, study subjects were classified into 16 groups (A∼J group; below 10 years with one year interval, K∼O group; 11~60 yrs with 10 years interval, p group; over 60 yrs). The numbers of each group were 100, and sex distributions of each group were almostly equal. And far the indirect assessment of tetanus immunization in Korean children, children under 15 years old age were classified into 6 groups (I∼VI) according to the status of DTaP vaccination. The numbers of this each group were 50, and sex ratio was almostly equal. Specific IgG antibody to tetanus toxin were detected by ELISA. And the ANOVA repeated t-test was used to compare antibody levels in study groups. Results : In age related groups, the antibody levels to tetanus toxin were well maintained until 20 years old age group (L group), but thereafter the titers abruptly decreased below 0.1 IU/mL and over 75% populations among the groups over 30 years old age needed maintenance of protective immunity to tetanus. The antibody level of male was statistically higher than that of female in P group. In the groups related DTaP vaccination status, the antibody titer was very low in pre-vaccination group (I), but the titers after primary vaccinations were sharply increased and highly maintained until 15 years. Conclusion : The results of our study revealed that the immunity to tetanus was dramatically decreased in age groups over 30 years old. This result indicates that Td vaccination program in adults should be considered for maintenance of immunity to tetanus. And our study indicate that DtaP vaccination programs and vaccines, used in Korea, are effective for acquisition and maintenance of tetanus immunity in Korean children. (Korean J Infect 33:104~111, 2001)
Jung Sook Sung,Ho Cheol Ko,On Sook Hur,Sang Gyu Kim,Jung Ro Lee,Binod P. Luitel,Jae Gyun Gwag,Hyung Jin Baek,Kyoung Yul Ryu 한국국제농업개발학회 2015 韓國國際農業開發學會誌 Vol.27 No.3
사우디아라비아 북부지역에서 수집된 홍화 총 100자원에 대 한 총폴리페놀 함량 및 항산화 활성을 평가하여 항산화활성이 높은 홍화자원을 선발하고자 연구를 수행하였으며 그 결과는 다음과 같다. 1. 총 100자원의 홍화유전자원에 대한 총폴리페놀 함량은 14.2 ± 0.41 μg GAE mg−1dw에서 81.6 ± 1.56μg GAEmg−1dw 까지 분포하였다. DPPH 라디컬 소거능은 1.6 ± 0.07 μg ASC mg−1 dw에서 14.1 ± 0.23 μg ASCmg−1 dw까지 활성을 나 타냈다. ABTS 활성은 34.5 ± 0.70 μg Trolox mg−1 dw에서 156.5 ± 3.06 μg Trolox mg−1 dw까지 평가되었다. 항산화활성 을 측정한 DPPH와 ABTS 활성간에는 상당한 유의성(r = 0.954**)을 보였다. 2. 홍화자원 100자원의 총폴리페놀 함량 및 항산화활성 결 과값에 대한 상호관계를 분석하여 활성이 높은 그룹(Group1)을 얻었다. Group1에 포함된 8개의 홍화자원은 나머지 그룹에 속한 자원들에 비해 항산화활성이 높았으며, 이중 K185841, K185879, K185863은 Group1내의 다른 자원들보다도 항산화 활성이 상당히 높아 홍화육종이나 기능성물질연구에 좋은 소 재가 될 것으로 사료된다. This study was undertaken to select genetic resources of safflower with high antioxidant activities. A total of 100 accessions were obtained from the National Agrobiodiversity Center (NAC) of Korea. Total polyphenol contents (TPC) and antioxidant activities of 1,1-diphenyl-2-picryl-hydrazil (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) in 75 % ethanol seed extract of safflower germplasm were determined. TPC ranged from 14.2 ± 0.41 to 81.6 ± 1.56 μg GAE mg-1dry weight (dw). Safflower seed extracts showed variation in DPPH antioxidant activities ranging from 1.6 ± 0.07 to 14.1 ± 0.23 μg ASC mg-1 dw. Antioxidant activities of ABTS ranged from 34.5 ± 0.70 to 156.5 ± 3.06 μg Trolox mg-1 dw. ABTS values showed significant positive correlation (r=0.954**) with DPPH activity. Group I (8 accessions) having high antioxidant activities were obtained by the matrix of calculated distances of TPC and antioxidant activities. K185841, K185879, and K185863 among group I germplasm had higher TPC and antioxidant activities than other accessions. These genotypes are potential sources of natural antioxidants. Therefore, this study provides valuable information for safflower breeders and growers to develop new varieties and produce functional foods.
On-Sook Hur,Sang Gyu Kim,Ho-Cheol Ko,Su Ran Ahn,Jung-Sook Sung,Na-Young Ro,Sukyeung Lee,Yu-mi Choi,Do yoon Hyun,Kyoung-Yul Ryu,Hyung-Jin Baek 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
This study aimed to evaluate 105 tomato accessions conserved in National Agrobiodiversity Center regarding their resistance to Ralstonia solanacearum, a soil-borne vascular bacterium that causes lethal wilt diseases of a wide range of crops worldwide. All the accessions are Solanum lycopericum var. lycopersicum including cultivar or breeding lines. At the four leaf stage, the seedlings were inoculated by drenching the soil with the bacterial suspension concentrated of 108 CFU/ml. Plant roots were wounded before inoculation by cutting with the knife. Seven accessions including IT 32899 were rated as resistant, while other 98 accessions were rated as susceptible. IT 32899 scored 0.1 of disease rate and 0.7 of disease index. The selected accessions will be used as a material to reveal the mechanism of wilt tolerance and to identify the host gene involved in defense response.
Comparison of Glucosinolates Contents in the Germplasm of Different Cruciferous Vegetables
On-Sook Hur,Jung-Bong Kim,Jung-Sook Sung,Sang Gyu Kim,Jae-Gyun Gwak,Sukyeung Lee,Yu-mi Choi,Do yoon Hyun,Myung-chul Lee,Hyung-Jin Baek,Binod Prasad Luitel,Kyoung-Yul Ryu,Ho-Cheol Ko 한국원예학회 2015 한국원예학회 학술발표요지 Vol.2015 No.10
수집지가 다른 홍화자원의 형태적 및 생화학적 특성 변이
Jung Sook Sung,Ho Cheol Ko,On Sook Hur,Sang Gyu Kim,Jung Ro Lee,Binod P. Luitel,Yong Hwa Lee,Young Seok Jang,Jae Gyun Gwag,Hyung Jin Baek,Kyoung Yul Ryu 한국국제농업개발학회 2016 韓國國際農業開發學會誌 Vol.28 No.1
One hundred seventy two accessions of safflower, collected in four countries were investigated for their morphological and biochemical characters in 2014. The accessions were categorized into two groups; South-Central (S-C) Asia and South-West (S-W) Asia, and each group was represented the accessions of two countries. Variation in morphological and biochemical characters was observed between two groups of accessions. The average value of seed weight and range of variability were higher in S-C Asia accessions while S-W accessions exhibited the variation in plant height, leaf length and days to flowering. The average value of oleic and total oil content were higher in S-C Asia accessions, and the values were 19.8%, and 231.4 mg.g-1, respectively, while the range of variability for total oil content was higher in S-W accessions. Plant height exhibited a significant positive correlation with days to flowering (r = 0.625**). Palmitic acid had positively correlated with stearic acid (r = 0.282**) and linoleic acid (r = 0.444**). Oleic and linoleic acid showed a strong negative correlation (r = -0.977**). The first three principal components explained 57% of the total variation. Morphological and biochemical variation exist in different groups of accessions could be useful to breeder for developing new safflower cultivars with high oil quantity and quality.
Allium ampeloprasum L. 유전자원의 항산화 활성 비교
Jung Sook Sung,Yi Jin Jeong,Da Jeong Kim,Jung Ro Lee,On Sook Hur,Ho Cheol Ko,Ju Hee Rhee,Hyung Jin Baek,Kyoung Yul Ryu 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : Garlic is one of the important vegetables and a source of natural anti-oxidants. This study was carried out to investigate the antioxidant activities including 2,2-diphenyl-1-picr yl-hydrazil (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), ferric reducing antioxidant power (FRAP), reducing power (RP) and total polyphenol content (TPC) of A. ampel oprasum L. Methods and Results : DPPH, ABTS, FRAP, TPC, and RP were analyzed the extract of aerial plant part in 22 accessions of A. ampeloprasum by spectrophotometry mathod. A. tuberosum Rottler ex Spreng. was used as a control. DPPH was ranged from 0.35 to 3.06 ㎍ ASC ㎎-1 dw. ABTS and FRAP showed wide variation from 4.37 to 29.30 ㎍ Trolox ㎎-1 dw and 3.18 to 10.8 ㎍ ASC ㎎-1 dw, respectively. RP and TPC were ranged from 3.43 to 9.51 ㎍ GAE ㎎-1 dw, and 0.8 to 18.63 ㎍ ASC ㎎-1 dw, respectively. Cluster analysis of A. ampeloprasum germplasm was divided into two major groups. Group Ⅰ (7 accessions) characterized as higher antioxidant activities than the group Ⅱ (15 accessions) and had similar antioxidant activities with A. tuberosum as a control. FRAP value showed a significant strong positive correlation with DPPH (r = 0.868*) and ABTS activity (r = 0.826*). Principal component analysis showed that the first two principal components (PC1 and PC2) cumulatively explained 85.64 % of total variation. Conclusion : From the above results, we may suggest that A. ampeloprasum aerial parts might have useful as a new material for functional food.