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( Sunghun Na ),( Jong Yun Hwang ),( Hyang Ah Lee ),( Songyi Kook ),( Jung Hwa Ko ),( Dong Hun Lee ),( Seok Ho Hong2 ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: Recently, perivascular cells(PVCs) around blood vessels turn out to be the origin of all MSCs and can be isolated from various tissues based on the expression of CD146 and recent evidence indicates that PVCs possess the greater proliferation and differentiation potential than bone marrow- derived MSCs. Therefore, efficient isolation and expansion of PVCs from various fetal and adult tissues is an essential tool for providing sufficient amount of PVCs for more than one treatment. In this study, human umbilical cord blood vessel present in the PVC can be efficiently separated and cultured to develop methods, and further the efficiency of proliferation and for improving the reproduction performance evaluation of various growth factors through the limitations of the existing MSCs the foundation wants more appropriate and effective treatment of the cell material that propose new alternatives. 방법: This study was approved by the Institutional Review Board of Kangwon National University Hospital Authority and in 2013, Kangwon National University Hospital 13 peoples who visited a normal pregnancy after cesarean section consent discarded umbilical cord was used. In harvested human umbilical cord, the two of umbilical arteries were separated. One umbilical artery was isolated PVCs by non-enzymatic isolation( NE) method and another umbilical artery was isolated PVCs by collagenase(COL) method. To confirm that cultured PVCs were the stem cells was observed by adipogenic differentiation, osteogenic differentiation, chonrogenic differentiation. Furthermore, proliferation of PVCs were compared by treatment of growth factors, flow cytometry, immunocytochemistry, Fluorescence activated cell sorting(FACS) using the method of immune phenotype was compared with conventional enzyme treatment. 결과: While both methods displayed similar patterns or levels of phenotypic expression and growth rate, NE method had an increased frequency of CD146(+) population compared to COL method over passage. We also found that supplementation of basic fibroblast growth factor(bFGF) significantly enhanced PVC proliferation by driving the cells into S phase and increased a proportion of Stage-specific embryonic angtigen(SSEA)-4 population within PVC cultures without alteration of other immunophenotype. 결론: Our findings suggest that a combination of NE method and bFGF supplementation can be an epoch-making way to isolate more homogeneous PVC population from human umbilical cord, which will enhance the utility of PVCs for cell therapy for various diseases.
Clinical and histological significance of atypical glandular cells on cervical cytology(초)
최홍준 ( Hong Jun Choi ),( Woo Chul Kim ),( Ho Soap Hahn ),( Seok Geun Yoon ),( Yong Soon Kwon ),( In Ho Lee ),( Kyung Taek Lim ),( Ki Heon Lee ),( Jae UK Shim ),( Jung Eun Mok ),( Yi Kyeong Chun2 ),( Ta 대한산부인과학회 2009 대한산부인과학회 학술대회 Vol.95 No.-